FastQCFastQC Report
Thu 26 May 2016
SRR937310_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937310_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82131
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8941.0885049494100887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.858384775541513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.5357295053025045No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.18994046097088796No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.1692418209932912No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1150.1400202116131546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.12297427280807491No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN940.11445130340553505No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT900.10958103517551229No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3400.055.903541
CGACCCG405.332637E-447.547045
TCCCGAC405.3487363E-447.518012
CCCGACC405.3487363E-447.518013
CAATGCA459.5548783E-442.238239
CCGACCC500.001599264838.0376324
CTCCCGA500.001604075938.0144121
GTATCAA5550.037.6719361
GTACAGA600.003922462531.6786751
GCTTTAT600.003922462531.6786751
ATGGGAG1252.0945481E-730.4301055
CTGGGAG650.005784780729.2597165
TATCAAC7950.026.8969862
TACATGG4600.024.7920062
GTACATG4650.024.5254251
CAACGCA8550.024.468655
ATCAACG8550.024.4537123
AACGCAG8650.024.1710136
GAGTACT6200.024.14221612-13
CATGGGA3550.024.1083564