##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937310_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82131 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03092620326064 33.0 31.0 34.0 31.0 34.0 2 32.17205440089613 33.0 31.0 34.0 31.0 34.0 3 32.18248895057895 34.0 31.0 34.0 30.0 34.0 4 35.790639344461894 37.0 35.0 37.0 35.0 37.0 5 35.79247787071873 37.0 35.0 37.0 35.0 37.0 6 35.86817401468386 37.0 36.0 37.0 35.0 37.0 7 35.83237754319319 37.0 36.0 37.0 35.0 37.0 8 35.83353423189782 37.0 36.0 37.0 35.0 37.0 9 37.50418234284253 39.0 38.0 39.0 35.0 39.0 10-11 37.42623369982102 39.0 37.0 39.0 35.0 39.0 12-13 37.3067477566327 39.0 37.0 39.0 35.0 39.0 14-15 38.50659312561639 40.0 38.0 41.0 34.5 41.0 16-17 38.49947035832999 40.0 38.0 41.0 34.5 41.0 18-19 38.550723843615685 40.0 38.0 41.0 35.0 41.0 20-21 38.5509125665096 40.0 38.0 41.0 35.0 41.0 22-23 38.39191048446993 40.0 38.0 41.0 34.0 41.0 24-25 38.25562211588803 40.0 38.0 41.0 34.0 41.0 26-27 38.089600759761844 40.0 38.0 41.0 34.0 41.0 28-29 38.06110360278092 40.0 38.0 41.0 34.0 41.0 30-31 37.81806504243221 40.0 37.5 41.0 34.0 41.0 32-33 37.48454907404025 40.0 36.5 41.0 33.0 41.0 34-35 37.2999111176048 40.0 36.0 41.0 33.0 41.0 36-37 37.24261241187858 40.0 36.0 41.0 33.0 41.0 38-39 37.09869598568142 40.0 36.0 41.0 33.0 41.0 40-41 36.79438336316373 40.0 36.0 41.0 32.0 41.0 42-43 36.387113270263356 40.0 35.0 41.0 31.0 41.0 44-45 35.94038791686452 40.0 35.0 41.0 29.5 41.0 46-47 35.31516723283535 39.5 35.0 41.0 25.5 41.0 48-49 34.74599724829845 39.0 35.0 41.0 20.5 41.0 50-51 33.75982881007172 38.0 34.0 40.0 13.5 40.5 52-53 33.72668663476641 38.5 34.5 40.0 8.0 40.5 54-55 33.95687986265844 39.0 35.0 40.0 3.5 41.0 56-57 33.74874894984842 39.0 35.0 41.0 2.0 41.0 58-59 33.452667080639465 38.5 34.0 40.0 2.0 41.0 60-61 33.14875016741547 38.0 34.0 40.0 2.0 41.0 62-63 32.774421351255924 38.0 34.0 40.0 2.0 41.0 64-65 32.36295065200716 37.5 33.0 40.0 2.0 41.0 66-67 31.942098598580316 37.0 33.0 40.0 2.0 41.0 68-69 31.475374706261945 36.5 32.0 39.0 2.0 41.0 70-71 30.991933618244026 36.0 31.5 39.0 2.0 41.0 72-73 30.56714273538615 35.5 31.0 39.0 2.0 40.0 74-75 30.139527096954865 35.0 31.0 38.0 2.0 40.0 76-77 29.714918849155616 35.0 30.0 37.0 2.0 39.5 78-79 29.254800258124217 35.0 30.0 37.0 2.0 39.0 80-81 28.80417869014136 35.0 29.0 36.0 2.0 39.0 82-83 28.37233200618524 34.0 29.0 36.0 2.0 37.5 84-85 27.95463345143734 34.0 28.0 35.0 2.0 37.0 86-87 27.520503829248398 34.0 26.5 35.0 2.0 36.5 88-89 27.12187846245632 34.0 26.0 35.0 2.0 36.0 90-91 26.688552434525334 34.0 25.0 35.0 2.0 36.0 92-93 26.228926958152222 34.0 24.5 35.0 2.0 35.0 94-95 25.663695803046352 33.0 20.5 35.0 2.0 35.0 96-97 25.04753381792502 33.0 12.5 35.0 2.0 35.0 98-99 24.28351657717549 32.0 2.0 35.0 2.0 35.0 100-101 22.564695425600565 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.14146457825165726 1101 2 0.07416552619705641 1101 3 -0.15247003079309707 1101 4 -0.10374659089134752 1101 5 0.13318761874657525 1101 6 -0.17174712882003007 1101 7 -0.0578992444540134 1101 8 -0.04676655725338463 1101 9 0.08406479772725817 1101 10-11 -0.25798096874859766 1101 12-13 -0.10415807374211283 1101 14-15 -0.2612821664935012 1101 16-17 -0.0031439260296011184 1101 18-19 0.16930527692431951 1101 20-21 -0.14023192375479 1101 22-23 0.08044129395161548 1101 24-25 -0.18417824326951404 1101 26-27 -0.05324628951785115 1101 28-29 -0.23790632683098067 1101 30-31 -0.016568440683521146 1101 32-33 -0.015206577990127812 1101 34-35 -0.019221560904249202 1101 36-37 0.33689927999830616 1101 38-39 0.03445644021920202 1101 40-41 -0.15808020077246 1101 42-43 0.23749902421020863 1101 44-45 0.6344424013481671 1101 46-47 0.4476311593461588 1101 48-49 0.7342384721290642 1101 50-51 0.9844229675061626 1101 52-53 1.5628366939861067 1101 54-55 1.3674436568887742 1101 56-57 0.8995115898738462 1101 58-59 1.0314918362089358 1101 60-61 1.1565721185681 1101 62-63 1.4980091190373699 1101 64-65 1.7894197603464015 1101 66-67 1.9682401332088482 1101 68-69 1.2782478272910858 1101 70-71 1.5443321308779687 1101 72-73 0.890756256982165 1101 74-75 0.6701686813829717 1101 76-77 0.7836141679456716 1101 78-79 0.9675693882630441 1101 80-81 0.9932614129225747 1101 82-83 0.7329692285408349 1101 84-85 1.0542113757311675 1101 86-87 1.4847340323223612 1101 88-89 1.3945399777771819 1101 90-91 1.014313728933299 1101 92-93 0.8856770712419824 1101 94-95 0.6886711757789428 1101 96-97 0.6245328909652166 1101 98-99 0.588965578566782 1101 100-101 0.7130746221761015 1102 1 0.27695647437992577 1102 2 0.17416552619705783 1102 3 0.4514773376279564 1102 4 0.20151656700338805 1102 5 0.18318761874657952 1102 6 0.33877918696944676 1102 7 0.4710481239670372 1102 8 0.2953387059045127 1102 9 0.5853805872009445 1102 10-11 0.34662429440929543 1102 12-13 0.5932103473105172 1102 14-15 0.8683230966643976 1102 16-17 0.7725139687072442 1102 18-19 0.81338422429274 1102 20-21 0.5979259709820539 1102 22-23 0.4982044518463482 1102 24-25 0.4414796514673256 1102 26-27 0.41451686837688584 1102 28-29 0.5232778836953287 1102 30-31 0.3893526119480626 1102 32-33 0.7874250009572421 1102 34-35 0.48669949172733595 1102 36-37 0.7178203326298842 1102 38-39 1.2449827560086746 1102 40-41 1.0182355887012235 1102 42-43 1.0802621821049456 1102 44-45 1.6844424013481643 1102 46-47 2.4140785277672023 1102 48-49 2.6467384721290657 1102 50-51 2.625212441190371 1102 52-53 2.233889325565052 1102 54-55 2.250996288467725 1102 56-57 2.0560905372422695 1102 58-59 2.7097813098931454 1102 60-61 2.9578879080417764 1102 62-63 2.8032722769321055 1102 64-65 2.8519197603464015 1102 66-67 3.6202138174193728 1102 68-69 3.5848267746595077 1102 70-71 3.9364373940358632 1102 72-73 3.144703625403224 1102 74-75 3.280037102435603 1102 76-77 3.225719431103567 1102 78-79 2.806385177736729 1102 80-81 2.804445623448892 1102 82-83 2.6336271232776767 1102 84-85 2.8871061125732744 1102 86-87 2.525523506006575 1102 88-89 2.77546103040876 1102 90-91 2.691945307880669 1102 92-93 2.8639665449261926 1102 94-95 3.066302754726312 1102 96-97 3.266638154123111 1102 98-99 3.10475505225099 1102 100-101 2.7058377800708406 1103 1 0.2110644716458161 1103 2 0.21394679755727708 1103 3 0.23056141144887476 1103 4 0.4198350905850745 1103 5 -0.08639542978384185 1103 6 -0.16798773032378733 1103 7 -0.26278646249912896 1103 8 0.3466032308532476 1103 9 -0.22856336358510987 1103 10-11 0.5293994140265141 1103 12-13 0.22444753117927974 1103 14-15 0.4474071704853131 1103 16-17 0.27347090650628303 1103 18-19 -0.030839972563029505 1103 20-21 -0.0020740290179475096 1103 22-23 -0.22052418793491313 1103 24-25 0.13054321947826253 1103 26-27 0.2676269162237688 1103 28-29 0.32553352279308 1103 30-31 0.3970081143404087 1103 32-33 0.387219942857449 1103 34-35 0.019098671494930386 1103 36-37 0.4316959307160104 1103 38-39 0.5412917102123629 1103 40-41 0.7187140576007423 1103 42-43 -0.09636119451842262 1103 44-45 0.9415169057910902 1103 46-47 0.9895399084917429 1103 48-49 1.332452757843356 1103 50-51 1.6801680119354145 1103 52-53 1.1815995101173442 1103 54-55 2.113128892705589 1103 56-57 1.5655232098328398 1103 58-59 1.3140875299888393 1103 60-61 1.9837252286159455 1103 62-63 2.592335844943044 1103 64-65 2.635925228562396 1103 66-67 2.5980675426415196 1103 68-69 3.1928923932514444 1103 70-71 2.73349822793881 1103 72-73 2.8928922788550295 1103 74-75 2.6062161865094247 1103 76-77 2.1099983101192876 1103 78-79 2.0596319309834854 1103 80-81 2.324131201029207 1103 82-83 2.8393003973720035 1103 84-85 2.503715818656662 1103 86-87 1.4152706010168252 1103 88-89 1.6971288362870922 1103 90-91 1.8758140706967978 1103 92-93 1.8500909468400657 1103 94-95 1.6400553179525588 1103 96-97 1.2995158027902356 1103 98-99 1.0397516346159925 1103 100-101 1.7720715463046055 1104 1 0.3585354217483463 1104 2 0.42942868409179624 1104 3 0.7620036534174304 1104 4 0.5673060406876047 1104 5 0.4226613029571027 1104 6 0.13088445012733985 1104 7 0.534206018703884 1104 8 0.04533870590451272 1104 9 0.27485427141147056 1104 10-11 0.3242558733566625 1104 12-13 0.724789294678942 1104 14-15 0.7814809914012386 1104 16-17 0.480408705549344 1104 18-19 0.5278579085032646 1104 20-21 0.5637154446662578 1104 22-23 0.4324149781621429 1104 24-25 0.6217428093620612 1104 26-27 0.7987273946926692 1104 28-29 0.7719620942216494 1104 30-31 1.2630368224743762 1104 32-33 1.0768986851677695 1104 34-35 1.2340679127799632 1104 36-37 1.1493992799983062 1104 38-39 1.4160353875876268 1104 40-41 1.1313934834380674 1104 42-43 1.392104287368113 1104 44-45 0.812073980295537 1104 46-47 1.2009206330303641 1104 48-49 2.0138437352869545 1104 50-51 1.8199492832956352 1104 52-53 2.0628366939861067 1104 54-55 2.318101551625624 1104 56-57 2.3403010635580657 1104 58-59 2.291360257261566 1104 60-61 2.9592036975154627 1104 62-63 2.80064069798474 1104 64-65 3.088761865609552 1104 66-67 3.1031085542614782 1104 68-69 3.5282478272910858 1104 70-71 3.1430163414042838 1104 72-73 3.6275983622453225 1104 74-75 3.5155634182250743 1104 76-77 3.7770352205772504 1104 78-79 4.2932272829998865 1104 80-81 3.0557614129225747 1104 82-83 3.21652186011978 1104 84-85 3.613421902046955 1104 86-87 3.063681400743416 1104 88-89 2.957039977777182 1104 90-91 3.185366360512244 1104 92-93 2.290282334399876 1104 94-95 2.8136711757789463 1104 96-97 3.3219013120178467 1104 98-99 3.6284392627773094 1104 100-101 3.611100937965574 1105 1 0.07470083528217586 1105 2 0.3730377066481836 1105 3 -0.44476326387579945 1105 4 -0.4843856886357045 1105 5 -0.328278546666958 1105 6 -0.9179877303237873 1105 7 -0.47707217678484426 1105 8 -0.4894357301857113 1105 9 0.20812494810319748 1105 10-11 -0.48683435220724647 1105 12-13 0.037759219490972384 1105 14-15 -0.19220321912507643 1105 16-17 -0.712730392195013 1105 18-19 -0.7061646478877108 1105 20-21 -0.1985026004465169 1105 22-23 -0.34390081131154204 1105 24-25 -0.19169704026199952 1105 26-27 -0.533509447412591 1105 28-29 -0.9423236200640659 1105 30-31 -0.6801022752699808 1105 32-33 0.04306409870159911 1105 34-35 0.279650619546878 1105 36-37 -0.5959014718813975 1105 38-39 -1.2427991988785436 1105 40-41 -1.0199223060356175 1105 42-43 -0.9697378178950515 1105 44-45 -0.685106470832288 1105 46-47 -0.5843237278718902 1105 48-49 -0.20326152787093577 1105 50-51 0.01214853141593153 1105 52-53 -0.05135503533720254 1105 54-55 -1.3755074709307706 1105 56-57 -1.6568793875697594 1105 58-59 -0.7711397427384341 1105 60-61 0.017004449395166432 1105 62-63 0.046881299488497064 1105 64-65 -0.761802044164881 1105 66-67 -2.0975493404753642 1105 68-69 -1.526263450904402 1105 70-71 -1.2380926811521036 1105 72-73 -1.4269129159501652 1105 74-75 -1.6916734238801858 1105 76-77 -1.7365925989716224 1105 78-79 -1.8900433936918404 1105 80-81 -1.5386934742954672 1105 82-83 -0.7759593428877345 1105 84-85 -0.36073223329139026 1105 86-87 -1.3014501782039538 1105 88-89 -2.707903631245376 1105 90-91 -1.5519456695629437 1105 92-93 -1.0419545077053876 1105 94-95 -1.41189273399549 1105 96-97 -1.812497184222753 1105 98-99 -1.2183652485008878 1105 100-101 -2.529876505643447 1106 1 0.33019534077668666 1106 2 0.4142464978569791 1106 3 0.7063356372230984 1106 4 0.5197351904851715 1106 5 0.3983698049813924 1106 6 0.15618809385203747 1106 7 0.49270804299538185 1106 8 0.2042455884956027 1106 9 0.2616963766746281 1106 10-11 0.21646235108945433 1106 12-13 -0.041911110179356115 1106 14-15 -0.16335706527892313 1106 16-17 -0.2807248976895238 1106 18-19 -0.21303277975584223 1106 20-21 0.6229259709820525 1106 22-23 0.5695607271499981 1106 24-25 0.2897590036940443 1106 26-27 0.2693751679720222 1106 28-29 0.4450390172985692 1106 30-31 -0.7577121653798784 1106 32-33 -0.1437490881115835 1106 34-35 0.52759017998644 1106 36-37 -1.1426047685846896 1106 38-39 -1.0161508472301932 1106 40-41 -1.2410761521894642 1106 42-43 -0.42166089481813174 1106 44-45 -0.31697460270041944 1106 46-47 0.29960484355667916 1106 48-49 0.556353856744451 1106 50-51 0.9894836962510993 1106 52-53 0.871035074552907 1106 54-55 0.09289912247582066 1106 56-57 0.11509863440826251 1106 58-59 -0.11248589658458741 1106 60-61 0.3185154384061448 1106 62-63 0.11693624454343876 1106 64-65 0.12418696682413 1106 66-67 0.40725835183232917 1106 68-69 0.10011017546922218 1106 70-71 0.10050621994679787 1106 72-73 1.285999171961926 1106 74-75 0.8419804222736609 1106 76-77 1.1171161922371695 1106 78-79 0.7583082546598057 1106 80-81 0.6336966355946423 1106 82-83 0.31469999777160496 1106 84-85 -0.32433113439028816 1106 86-87 0.05912674487296954 1106 88-89 -0.44073330157504387 1106 90-91 -0.7174676475849218 1106 92-93 -0.31599296924384745 1106 94-95 0.14717320007044066 1106 96-97 0.07898633226076157 1106 98-99 -1.0466619517975921 1106 100-101 -0.8499314506983957 1107 1 -0.5184668665811749 1107 2 -0.4452852747182767 1107 3 -0.6373098477267334 1107 4 -1.1575223345984398 1107 5 -0.752967987660746 1107 6 0.03077003365136477 1107 7 -1.2472585121885658 1107 8 -1.4964233078254665 1107 9 -0.9448253624558092 1107 10-11 -0.5209958428904784 1107 12-13 -0.3123960600121336 1107 14-15 0.014318520005360824 1107 16-17 -0.6168452990273181 1107 18-19 -0.1005745857759095 1107 20-21 -0.4912044638005568 1107 22-23 -0.08303124609415136 1107 24-25 -0.42888648125578754 1107 26-27 -0.19383553436910717 1107 28-29 -0.7552118188218344 1107 30-31 -0.3124780516674974 1107 32-33 -0.47401664664001686 1107 34-35 -1.2424767096456648 1107 36-37 -1.4289760060428804 1107 38-39 -1.221836465959285 1107 40-41 -1.4384005669051803 1107 42-43 -0.6762595570254888 1107 44-45 -0.5903859739378774 1107 46-47 -0.8688734173128836 1107 48-49 -1.192391962653545 1107 50-51 -0.7258173070933793 1107 52-53 -0.8467742899955866 1107 54-55 -1.3343584026077906 1107 56-57 -0.7415067167623022 1107 58-59 -0.33907452534712945 1107 60-61 -1.641768842530304 1107 62-63 -1.5757895079649202 1107 64-65 -1.2361809261524606 1107 66-67 -1.6220058622144968 1107 68-69 -1.1528162459354583 1107 70-71 -1.2268349171769515 1107 72-73 -0.7382483196768703 1107 74-75 -0.8302603804019242 1107 76-77 -0.7152416672946025 1107 78-79 -0.21535395269805235 1107 80-81 -0.029764902866897813 1107 82-83 0.2682953154973582 1107 84-85 0.024749133168239013 1107 86-87 0.5490933000569136 1107 88-89 0.4873603439099057 1107 90-91 0.04549221863581465 1107 92-93 0.29771940533808916 1107 94-95 0.9017718622777977 1107 96-97 2.011260579752399 1107 98-99 0.7886223291388639 1107 100-101 0.6145334322447518 1108 1 -1.3925610694797257 1108 2 -1.3710099123994333 1108 3 -0.8465489781615148 1108 4 -1.2462904505404708 1108 5 -1.2747071180955274 1108 6 -1.048940111276167 1108 7 -0.5008817005943627 1108 8 -0.4239595397095215 1108 9 -0.8633036233253719 1108 10-11 -0.24427482839772097 1108 12-13 -0.4265264947947429 1108 14-15 -0.22345321912507643 1108 16-17 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-2.1808852945158783 2316 86-87 -2.027215484326401 2316 88-89 -2.061219957775986 2316 90-91 -1.828731383848659 2316 92-93 -1.4769034872972249 2316 94-95 -2.192504978893453 2316 96-97 -2.0344359597329564 2316 98-99 -2.0270387178886438 2316 100-101 -2.0757948729903895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 383.0 3 76.0 4 45.0 5 48.0 6 53.0 7 69.0 8 92.0 9 87.0 10 145.0 11 186.0 12 334.0 13 494.0 14 594.0 15 912.0 16 1243.0 17 1711.0 18 1476.0 19 810.0 20 811.0 21 747.0 22 774.0 23 796.0 24 851.0 25 874.0 26 883.0 27 854.0 28 945.0 29 1099.0 30 1345.0 31 1634.0 32 2118.0 33 2963.0 34 4185.0 35 6482.0 36 10282.0 37 16384.0 38 16482.0 39 2864.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.396364612961904 20.132573214351112 15.881929269870723 20.589132902816264 2 14.707605193790881 21.21570828858733 41.27452894659768 22.802157571024114 3 18.262026212383763 26.633392722036465 31.858785053571864 23.245796012007908 4 11.649193892868912 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31.331524224776658 20.722627069549045 50-51 19.29477914585582 28.80281990766998 31.308659141221025 20.593741805253178 52-53 19.293655585438888 28.6867799793866 31.1237962810497 20.895768154124813 54-55 18.93671905014544 28.57003469871877 31.137983815395497 21.35526243574029 56-57 19.172469299903664 28.23128422130898 31.187273636330374 21.408972842456983 58-59 19.505740748644843 27.595836661524487 31.38662097644555 21.51180161338512 60-61 19.059116183786948 26.982733391864212 32.39318115305824 21.564969271290607 62-63 18.768484020659418 26.35600300012805 32.99957925033385 21.87593372887867 64-65 18.57242153013194 26.202351047484328 33.32439089822696 21.90083652415677 66-67 18.465675245568786 26.02571841445792 33.14614620107678 22.362460138896513 68-69 18.47873138195719 25.338218886605983 33.234159040610656 22.948890690826175 70-71 18.24407139329118 25.438280902233636 33.199587787283605 23.118059917191587 72-73 17.84300932334128 25.303511649603344 33.29756032122539 23.555918705829985 74-75 17.908973389594866 25.089026561623456 32.966657723359106 24.03534232542257 76-77 17.720623654733814 24.78521472430046 33.29126041914379 24.20290120182194 78-79 17.68199362206789 24.76722986774632 33.34999969512753 24.200776815058262 80-81 17.26534917895841 24.23856243025872 33.17764132708126 25.318447063701612 82-83 17.50243902439024 23.276829268292683 33.4640243902439 25.756707317073168 84-85 17.615784210911396 23.122698339145916 33.48230617271907 25.779211277223617 86-87 17.882684323157683 22.80650412452216 33.12725964674824 26.183551905571917 88-89 16.90617435997488 22.20196686928794 33.07401062085028 27.817848149886903 90-91 17.291921981794125 22.790266625206538 33.93877314603111 25.979038246968226 92-93 16.507938443528523 23.822647123381216 33.21403311952784 26.45538131356242 94-95 16.283054185390007 24.934912902183388 31.493009615208734 27.289023297217867 96-97 16.980349837518823 25.70494881690536 30.46781165596669 26.84688968960913 98-99 17.72051560346825 25.379568541847053 29.768539408056 27.131376446628703 100-101 18.92783329167251 24.225261091432298 29.164812251940276 27.682093364954913 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 106.0 1 65.5 2 29.0 3 29.0 4 20.5 5 19.5 6 48.0 7 111.0 8 191.5 9 252.0 10 232.5 11 191.5 12 185.5 13 196.5 14 218.0 15 243.5 16 262.5 17 302.0 18 324.5 19 353.5 20 404.5 21 431.5 22 459.0 23 507.0 24 598.5 25 701.0 26 779.0 27 890.5 28 1040.5 29 1151.5 30 1307.5 31 1488.5 32 1615.5 33 1758.0 34 1936.0 35 2083.5 36 2260.0 37 2475.5 38 2586.5 39 2660.0 40 2695.0 41 2776.5 42 2909.5 43 2967.5 44 3098.0 45 3191.5 46 3168.5 47 3250.0 48 3400.5 49 3289.0 50 2978.0 51 2714.5 52 2492.5 53 2288.0 54 2044.5 55 1748.5 56 1450.0 57 1223.5 58 1019.5 59 781.0 60 621.5 61 478.0 62 327.5 63 224.0 64 146.0 65 109.0 66 78.5 67 54.0 68 41.5 69 25.5 70 13.0 71 13.0 72 12.0 73 9.0 74 8.0 75 5.5 76 4.5 77 1.5 78 0.0 79 0.5 80 1.0 81 0.5 82 0.5 83 0.5 84 1.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26055935030621813 2 0.2276850397535644 3 0.22524990563855307 4 0.23620800915610424 5 0.2337728750410929 6 0.17167695510830258 7 0.16558911982077412 8 0.17045938805079688 9 0.16558911982077412 10-11 0.1838526256833595 12-13 0.1783735739245839 14-15 0.17472087275206682 16-17 0.16741547040703267 18-19 0.16315398570576276 20-21 0.16558911982077412 22-23 0.16558911982077412 24-25 0.15828371747573997 26-27 0.1558485833607286 28-29 0.16863303746453834 30-31 0.17106817157954973 32-33 0.16802425393578552 34-35 0.17593843980957252 36-37 0.15950128453324566 38-39 0.15645736688948145 40-41 0.15950128453324566 42-43 0.16437155276326842 44-45 0.16498033629202127 46-47 0.16376276923451558 48-49 0.16863303746453834 50-51 0.17350330569456113 52-53 0.17715600686707822 54-55 0.16985060452204404 56-57 0.1558485833607286 58-59 0.15767493394698714 60-61 0.14976074807320014 62-63 0.16376276923451558 64-65 0.15219588218821153 66-67 0.15523979983197575 68-69 0.1467168304294359 70-71 0.16376276923451558 72-73 0.1613276351195042 74-75 0.16193641864825706 76-77 0.15889250100449281 78-79 0.15767493394698714 80-81 0.1601100680619985 82-83 0.15950128453324566 84-85 0.15219588218821153 86-87 0.1479343974869416 88-89 0.1491519645444473 90-91 0.15158709865945869 92-93 0.15219588218821153 94-95 0.15280466571696438 96-97 0.1479343974869416 98-99 0.15828371747573997 100-101 0.1454992633719302 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 82131.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.35417808135783 #Duplication Level Percentage of deduplicated Percentage of total 1 91.76107876737956 74.651471429789 2 5.161860005687175 8.398777562674265 3 1.3050570962479608 3.1851554224348906 4 0.6121196701438257 1.9919397060793123 5 0.3202777736204858 1.3027967515310905 6 0.2379633925498002 1.1615589728604303 7 0.146669260816858 0.8352510014489047 8 0.09877725728482273 0.6428754063630054 9 0.073334630408429 0.5369470723600102 >10 0.25891614409506564 3.4371918033385693 >50 0.013469625993384917 0.7512388744810121 >100 0.007483125551880509 1.1579062716879132 >500 0.002993250220752204 1.9468897249516017 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 894 1.0885049494100887 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 705 0.858384775541513 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 440 0.5357295053025045 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 156 0.18994046097088796 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.1692418209932912 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 115 0.1400202116131546 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.12297427280807491 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 94 0.11445130340553505 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 90 0.10958103517551229 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0012175670575056922 0.0 20-21 0.0 0.0 0.0 0.0012175670575056922 0.0 22-23 0.0 0.0 0.0 0.0018263505862585383 0.0 24-25 0.0 0.0 0.0 0.004870268230022769 0.0 26-27 0.0 0.0 0.0 0.005479051758775615 0.0 28-29 0.0 0.0 0.0 0.019481072920091075 0.0 30-31 0.0 0.0 0.0 0.03409187761015938 0.0 32-33 0.0 0.0 0.0 0.05235538347274476 0.0 34-35 0.0 0.0 0.0 0.06940132227782445 0.0 36-37 0.0 0.0 0.0 0.09009996225542122 0.0 38-39 0.0 0.0 0.0 0.12845332456685052 0.0 40-41 0.0 0.0 0.0 0.2368167926848571 0.0 42-43 0.0 0.0 0.0 0.4054498301493955 0.0 44-45 0.0 0.0 0.0 0.5740828676139338 0.0 46-47 0.0 0.0 0.0 1.0191036271322642 0.0 48-49 0.0 0.0 0.0 1.4300325090404353 0.0 50-51 0.0 0.0 0.0 1.72407495342806 0.0 52-53 0.0 0.0 0.0 2.1289160000487026 0.0 54-55 0.0 0.0 0.0 2.5130584066917487 0.0 56-57 0.0 0.0 0.0 3.6015633561018374 0.0 58-59 0.0 0.0 0.0 4.79356150539991 0.0 60-61 0.0 0.0 0.0 6.123753515724879 0.0 62-63 0.0 0.0 0.0 7.782079848047632 0.0 64-65 0.0 0.0 0.0 9.374657559265076 0.0 66-67 0.0 0.0 0.0 11.263712848985158 0.0 68-69 0.0 0.0 0.0 13.20634108923549 0.0 70-71 0.0 0.0 0.0 15.10574569894437 0.0 72-73 0.0 0.0 0.0 17.081248249747354 0.0 74-75 0.0 0.0 0.0 19.25156152975125 0.0 76-77 0.0 0.0 0.0 22.100059660785817 0.0 78-79 0.0 0.0 0.0 24.268546590203457 0.0 80-81 0.0 0.0 0.0 26.963631271992305 0.0 82-83 0.0 0.0 0.0 30.297938658971646 0.0 84-85 0.0 0.0 0.0 34.40600991099585 0.0 86-87 0.0 0.0 0.0 37.63499774750095 0.0 88-89 0.0 0.0 0.0 41.19394625659008 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 340 0.0 55.90354 1 CGACCCG 40 5.332637E-4 47.54704 5 TCCCGAC 40 5.3487363E-4 47.51801 2 CCCGACC 40 5.3487363E-4 47.51801 3 CAATGCA 45 9.5548783E-4 42.23823 9 CCGACCC 50 0.0015992648 38.037632 4 CTCCCGA 50 0.0016040759 38.014412 1 GTATCAA 555 0.0 37.671936 1 GTACAGA 60 0.0039224625 31.678675 1 GCTTTAT 60 0.0039224625 31.678675 1 ATGGGAG 125 2.0945481E-7 30.430105 5 CTGGGAG 65 0.0057847807 29.259716 5 TATCAAC 795 0.0 26.896986 2 TACATGG 460 0.0 24.792006 2 GTACATG 465 0.0 24.525425 1 CAACGCA 855 0.0 24.46865 5 ATCAACG 855 0.0 24.453712 3 AACGCAG 865 0.0 24.171013 6 GAGTACT 620 0.0 24.142216 12-13 CATGGGA 355 0.0 24.108356 4 >>END_MODULE