FastQCFastQC Report
Thu 26 May 2016
SRR937310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937310_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82131
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.823075330873848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.7147118627558412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.4541525124496231No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.25812421619120673No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1960.23864314327111563No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.16437155276326842No Hit
GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC1010.12297427280807491No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC970.11810400457805212No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA950.11566887046304075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG407.4326344E-659.4432145
AGATTGA250.00500612456.9959876
TAGGATT352.7736503E-454.2818837
AGGTAAG451.4933592E-552.8384132
GGTAAGG405.32853E-447.5545733
GTAAGGT502.7849424E-547.5545734
AAGGTGT502.805106E-547.4966556
GGTATCA5050.043.3434641
CTATACT459.5188466E-442.270734
TAGCCAG459.5188466E-442.270735
CCCTGAG550.00256664334.543027
GTATCAA7850.033.944981
AAGGTAA804.4120522E-429.7397421
GGTGTGC650.005814933729.2287088
ATAGGAT650.005814933729.2287086
GTGTGCA650.005814933729.2287089
TATCAAC9150.029.1044392
GTGCACT504.9300135E-528.49799310-11
ATCAACG9700.027.9444393
TACAGAG700.00830037427.1740425