##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937310_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82131 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.369044575129976 33.0 31.0 34.0 31.0 34.0 2 32.58424955254411 34.0 31.0 34.0 31.0 34.0 3 32.65477103651484 34.0 31.0 34.0 31.0 34.0 4 36.24267329023146 37.0 35.0 37.0 35.0 37.0 5 36.14829966760419 37.0 35.0 37.0 35.0 37.0 6 36.260340188235865 37.0 37.0 37.0 35.0 37.0 7 36.22688144549561 37.0 37.0 37.0 35.0 37.0 8 36.21505886936723 37.0 37.0 37.0 35.0 37.0 9 37.95118773666459 39.0 38.0 39.0 35.0 39.0 10-11 37.875418538676016 39.0 38.0 39.0 35.0 39.0 12-13 37.81318259853161 39.0 38.0 39.0 35.0 39.0 14-15 39.03128538554261 40.0 38.0 41.0 36.0 41.0 16-17 39.0329473645761 40.0 38.0 41.0 36.0 41.0 18-19 39.08088906746539 40.0 38.5 41.0 36.0 41.0 20-21 39.04082502343817 40.0 38.0 41.0 36.0 41.0 22-23 38.919031790675874 40.0 38.0 41.0 35.0 41.0 24-25 38.75811812835592 40.0 38.0 41.0 35.0 41.0 26-27 38.54406375181113 40.0 38.0 41.0 34.5 41.0 28-29 38.51702158746393 40.0 38.0 41.0 35.0 41.0 30-31 38.54874529714724 40.0 38.0 41.0 35.0 41.0 32-33 38.41817340590033 40.0 38.0 41.0 35.0 41.0 34-35 38.27324031121014 40.0 38.0 41.0 34.5 41.0 36-37 38.08081601344194 40.0 37.5 41.0 34.0 41.0 38-39 37.84550900390839 40.0 37.0 41.0 34.0 41.0 40-41 37.63605703084097 40.0 37.0 41.0 33.0 41.0 42-43 37.324232019578474 40.0 37.0 41.0 33.0 41.0 44-45 36.83741218297598 40.0 36.5 41.0 32.0 41.0 46-47 36.2854646844675 40.0 36.0 41.0 30.5 41.0 48-49 35.75674227758094 40.0 35.5 41.0 28.5 41.0 50-51 35.41778987227721 39.5 35.0 41.0 27.0 41.0 52-53 35.11029939973944 39.0 35.0 41.0 25.0 41.0 54-55 34.879442597801074 39.0 35.0 41.0 24.0 41.0 56-57 34.60134419403148 39.0 35.0 40.0 22.5 41.0 58-59 34.275864168219066 38.5 35.0 40.0 20.0 41.0 60-61 33.89990381220245 38.0 34.0 40.0 16.0 41.0 62-63 33.51886620155605 38.0 34.0 40.0 7.5 41.0 64-65 33.13354884270251 37.0 34.0 40.0 2.0 41.0 66-67 32.68372478114232 37.0 33.5 39.5 2.0 41.0 68-69 32.19382449988433 36.0 33.0 39.0 2.0 41.0 70-71 31.77575458718389 36.0 32.5 39.0 2.0 40.0 72-73 31.304890966869998 35.0 32.0 38.5 2.0 40.0 74-75 30.888282134638565 35.0 31.5 37.5 2.0 40.0 76-77 29.61328243903033 34.0 30.0 36.0 2.0 39.0 78-79 30.133025288867785 35.0 31.0 36.0 2.0 39.0 80-81 29.930428218334125 35.0 31.0 36.0 2.0 38.5 82-83 29.6146643776406 35.0 31.0 36.0 2.0 37.0 84-85 29.292161303283777 34.5 31.0 35.0 2.0 37.0 86-87 28.972525599347385 34.0 30.0 35.0 2.0 36.5 88-89 28.66695279492518 34.0 30.0 35.0 2.0 36.0 90-91 28.301627887155885 34.0 29.0 35.0 2.0 35.5 92-93 28.011177265587904 34.0 29.0 35.0 2.0 35.0 94-95 27.706834203893777 34.0 29.0 35.0 2.0 35.0 96-97 27.359249248152345 34.0 28.0 35.0 2.0 35.0 98-99 26.838313182598533 33.5 26.5 35.0 2.0 35.0 100-101 25.480926812044174 32.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11718233641983744 1101 2 -0.02020196437262456 1101 3 0.13816976857672358 1101 4 0.18024111208038818 1101 5 -0.13231765625155845 1101 6 -0.05843858366068844 1101 7 -0.0071047568635194125 1101 8 -0.058102524143592404 1101 9 0.054616708267161584 1101 10-11 -0.13046087226865666 1101 12-13 -0.2015927685373029 1101 14-15 -0.4152075297206821 1101 16-17 -0.24422122836065085 1101 18-19 0.15801639183497684 1101 20-21 0.009064876806966993 1101 22-23 -0.06996089961999985 1101 24-25 -0.11956065870602117 1101 26-27 0.03659790738442581 1101 28-29 -0.0881920401767573 1101 30-31 0.12750297603859906 1101 32-33 0.15518099169929656 1101 34-35 0.6260023758208533 1101 36-37 0.554722789903245 1101 38-39 0.2856028391787646 1101 40-41 0.3715522711475643 1101 42-43 -0.008347604706678169 1101 44-45 -0.8342954234918096 1101 46-47 -0.3269946520730258 1101 48-49 0.024219543395595622 1101 50-51 -0.9114798290358479 1101 52-53 -0.7834810977853977 1101 54-55 -0.5038590820234035 1101 56-57 -0.17212242542039036 1101 58-59 -0.512776778636642 1101 60-61 -0.3172430629002818 1101 62-63 -0.42533767195422456 1101 64-65 0.0162983654904707 1101 66-67 -0.5424539327963238 1101 68-69 0.24590148324525885 1101 70-71 0.11806954448077889 1101 72-73 0.00458517626222843 1101 74-75 0.1585101536440483 1101 76-77 -0.4123146071009707 1101 78-79 -0.4925277151986265 1101 80-81 -0.11490016462924046 1101 82-83 -0.7361793535692627 1101 84-85 -0.5193578275192685 1101 86-87 -0.8012743277174081 1101 88-89 -0.3888608010808525 1101 90-91 -0.3606020486939592 1101 92-93 -0.4603084512010618 1101 94-95 -0.5625873888109574 1101 96-97 -0.9683171554303875 1101 98-99 -1.5227827011604163 1101 100-101 -0.9965839128738345 1102 1 0.39612970484088805 1102 2 0.125850667206322 1102 3 0.16974871594514696 1102 4 0.30787269102775383 1102 5 0.21241918585370456 1102 6 -0.1610701626080555 1102 7 0.13894787471542713 1102 8 0.09847642322483097 1102 9 0.34145881353032337 1102 10-11 0.3873022856260775 1102 12-13 0.1701177577784918 1102 14-15 0.45058194396353457 1102 16-17 0.22880508742882455 1102 18-19 0.26130586551919066 1102 20-21 0.45445961364907816 1102 22-23 0.20898646880105787 1102 24-25 0.2429393412939831 1102 26-27 0.40699264422652703 1102 28-29 0.5789132229811358 1102 30-31 0.3748713970912334 1102 32-33 0.5295230969624569 1102 34-35 0.9174497442418996 1102 36-37 0.8441964741137724 1102 38-39 1.0500765233892935 1102 40-41 1.2202364816738793 1102 42-43 1.8508629216091137 1102 44-45 2.0610993133502973 1102 46-47 2.104584295295396 1102 48-49 2.824219543395607 1102 50-51 3.126678065700986 1102 52-53 2.827703112740913 1102 54-55 3.0264040758713335 1102 56-57 3.1127459956322454 1102 58-59 3.4977495371528278 1102 60-61 3.4439411476260275 1102 62-63 3.4601886438352523 1102 64-65 3.4287983654904792 1102 66-67 3.6332039619405165 1102 68-69 3.9445856937715753 1102 70-71 3.5522800707965665 1102 72-73 3.580900965735914 1102 74-75 3.806536469433521 1102 76-77 2.8863696034253437 1102 78-79 3.3364196532224213 1102 80-81 3.2653629932654944 1102 82-83 3.401320646430733 1102 84-85 3.539852698796519 1102 86-87 3.314515145966805 1102 88-89 3.0131128831296756 1102 90-91 2.1183453197270943 1102 92-93 2.368638917219993 1102 94-95 2.680833663820625 1102 96-97 3.492209160359085 1102 98-99 2.713401509365898 1102 100-101 1.0876266134419552 1103 1 0.20228144783474278 1103 2 0.2647433534674306 1103 3 -0.157113895127992 1103 4 -0.12999470473437924 1103 5 -0.2357011148981769 1103 6 -0.09996284886916129 1103 7 0.20470317204966904 1103 8 -0.02742925004926633 1103 9 0.055471117016310245 1103 10-11 -0.09102990849558523 1103 12-13 -0.06330671248807818 1103 14-15 0.16801188244610188 1103 16-17 -0.21179641633057855 1103 18-19 0.2494808484310198 1103 20-21 0.2068177817967154 1103 22-23 0.5947691072152779 1103 24-25 0.2976898539392252 1103 26-27 0.2144430885190758 1103 28-29 0.19231035216773762 1103 30-31 0.6647551975013499 1103 32-33 0.8274725159645087 1103 34-35 0.5540184387053344 1103 36-37 1.0287487639292223 1103 38-39 0.6103636047290095 1103 40-41 0.9071811159869299 1103 42-43 1.334868389825111 1103 44-45 1.5437377275676596 1103 46-47 1.5236546985763226 1103 48-49 1.9753473629444755 1103 50-51 1.5824538688452137 1103 52-53 1.5989949787696247 1103 54-55 1.9136904736840492 1103 56-57 1.8193762075256146 1103 58-59 2.238419393612155 1103 60-61 1.9915829794783875 1103 62-63 2.262717693732725 1103 64-65 1.6093861987098848 1103 66-67 1.9727118225010116 1103 68-69 2.0205938960955656 1103 70-71 2.4066888199421577 1103 72-73 2.503047240513766 1103 74-75 1.8779650408620903 1103 76-77 2.061284162550429 1103 78-79 2.0848817174739622 1103 80-81 1.8103390698205182 1103 82-83 2.5034395801286173 1103 84-85 2.2185950091177773 1103 86-87 2.022033942959286 1103 88-89 1.963283764879506 1103 90-91 2.409049352536389 1103 92-93 1.9369916171516408 1103 94-95 2.2293640807720934 1103 96-97 1.5667136032845796 1103 98-99 1.171296246208005 1103 100-101 2.1719054924576753 1104 1 -0.014396610948580246 1104 2 0.3811138251010604 1104 3 0.16448555805040854 1104 4 -0.10265362476171447 1104 5 -0.36915976151471597 1104 6 -0.24264910997647604 1104 7 -0.06631528317931412 1104 8 0.04058168638272264 1104 9 0.1072482872145315 1104 10-11 0.2083549172050283 1104 12-13 0.40695986304165643 1104 14-15 0.22952931238458518 1104 16-17 0.4761735084814518 1104 18-19 0.4902532339402441 1104 20-21 0.2557754031227546 1104 22-23 0.31819699511684973 1104 24-25 0.265307762346616 1104 26-27 0.17804527580547358 1104 28-29 0.043386907191660384 1104 30-31 0.4827661339333389 1104 32-33 0.17162836012035143 1104 34-35 0.25429184950505856 1104 36-37 0.8507754214821901 1104 38-39 0.8513923128629841 1104 40-41 0.8057627974633519 1104 42-43 0.9719155531880617 1104 44-45 1.208467734402923 1104 46-47 1.7322158742427618 1104 48-49 2.2347458591850753 1104 50-51 2.3319412235957273 1104 52-53 2.5487557443198696 1104 54-55 2.2527198653450213 1104 56-57 1.9627459956322468 1104 58-59 2.1714337476791385 1104 60-61 1.699204305520766 1104 62-63 1.3246623280457754 1104 64-65 1.2893246812799504 1104 66-67 1.335835540887885 1104 68-69 1.989322535876834 1104 70-71 1.4272800707965736 1104 72-73 1.5703746499464444 1104 74-75 1.188115416801942 1104 76-77 0.9034748665832382 1104 78-79 0.9153670216434762 1104 80-81 1.5298366774760233 1104 82-83 1.4447416990623196 1104 84-85 1.691168488270204 1104 86-87 1.8368835670194343 1104 88-89 1.6473234094454625 1104 90-91 1.645976898674462 1104 92-93 1.7107441803778904 1104 94-95 1.503202084873255 1104 96-97 1.564577581411715 1104 98-99 2.3028751935764262 1104 100-101 3.1744687187051106 1105 1 -0.02661465606136204 1105 2 -0.38298391925984276 1105 3 -0.24152947954357984 1105 4 -0.1543453540850237 1105 5 -0.5749868291838851 1105 6 0.012050138143827382 1105 7 -0.3586085162620165 1105 8 -0.17840327602328898 1105 9 -0.5581652466200566 1105 10-11 -0.4514195188852028 1105 12-13 -0.883923595604962 1105 14-15 -0.22159850716428053 1105 16-17 -0.7564392734734326 1105 18-19 -0.7635061645559986 1105 20-21 -0.21039001041107497 1105 22-23 -0.12682180187563574 1105 24-25 -0.17877118502180878 1105 26-27 -0.1086088595328718 1105 28-29 -0.13236497250758106 1105 30-31 -0.15586168561552682 1105 32-33 0.42162836012035143 1105 34-35 0.11895350364039814 1105 36-37 -0.2934914958110397 1105 38-39 -0.03817535630994939 1105 40-41 -0.9564552476494299 1105 42-43 -0.7057160257593083 1105 44-45 -1.0025285061985727 1105 46-47 -0.5144946520730258 1105 48-49 -1.7389383513412398 1105 50-51 -2.432156520765176 1105 52-53 -2.2613946316199858 1105 54-55 -1.8168290068354338 1105 56-57 -1.0945848314354194 1105 58-59 -1.2331715154787517 1105 60-61 -1.8501377997423916 1105 62-63 -1.7843602283452 1105 64-65 -1.8873670480433589 1105 66-67 -2.3121907749015875 1105 68-69 -2.7545684415667715 1105 70-71 -2.658246244992906 1105 72-73 -2.8102644477979197 1105 74-75 -2.3095349591379097 1105 76-77 -2.1505664868002192 1105 78-79 -2.540929970837727 1105 80-81 -2.5946933977119464 1105 82-83 -2.8123071731181355 1105 84-85 -2.438060835038069 1105 86-87 -1.7815374856121444 1105 88-89 -2.113014936419198 1105 90-91 -1.5438727253856896 1105 92-93 -1.5199889023288797 1105 94-95 -1.3233956594876446 1105 96-97 -1.5566630200920422 1105 98-99 -0.8376323252205644 1105 100-101 -1.1836139880618042 1106 1 0.07366006921336066 1106 2 -0.0024894137653319603 1106 3 -0.17559541360951414 1106 4 0.195929371189699 1106 5 0.09946372026666239 1106 6 0.16726991836360128 1106 7 0.186721154067655 1106 8 0.4575857349657184 1106 9 0.3511754127206075 1106 10-11 0.13649256902688478 1106 12-13 -0.14010491428629024 1106 14-15 0.26191797635220126 1106 16-17 0.47364314410898345 1106 18-19 0.13347185742202328 1106 20-21 0.3995000994790274 1106 22-23 -0.02998114253497164 1106 24-25 -8.865696371955778E-4 1106 26-27 0.0479845470605369 1106 28-29 0.29895370881109073 1106 30-31 0.36130864405479457 1106 32-33 0.15239759088957783 1106 34-35 0.03516229484919364 1106 36-37 0.21818432836477797 1106 38-39 0.4267971711625762 1106 40-41 0.4707425545483659 1106 42-43 0.7750532050099253 1106 44-45 0.755026438856369 1106 46-47 0.8653130402346676 1106 48-49 1.2995231871203003 1106 50-51 1.275261061652408 1106 52-53 0.782561412336058 1106 54-55 0.5128413228348947 1106 56-57 0.7405800037294128 1106 58-59 1.300482330675095 1106 60-61 1.6162083541037617 1106 62-63 1.314540870555902 1106 64-65 1.2802153695390572 1106 66-67 1.4618476866368795 1106 68-69 1.676063426565097 1106 70-71 2.1975229857763203 1106 72-73 1.9053948928614233 1106 74-75 1.9507672386642874 1106 76-77 1.766329117595383 1106 78-79 0.8910755236677694 1106 80-81 0.5131362726177251 1106 82-83 0.29140161809065646 1106 84-85 0.620318285841055 1106 86-87 1.0261548220801622 1106 88-89 1.1878092394049773 1106 90-91 0.5756327691198067 1106 92-93 0.17025835041837567 1106 94-95 0.6772911536991693 1106 96-97 1.671358957929936 1106 98-99 1.834757784669545 1106 100-101 1.6790233745755572 1107 1 -0.5258382585458357 1107 2 0.07276142235506455 1107 3 -0.05519407581687119 1107 4 0.40077886951745967 1107 5 0.037590810567664334 1107 6 0.2224538648519001 1107 7 -0.5891985783738178 1107 8 -0.08756476670652091 1107 9 -0.20568077457036793 1107 10-11 -0.09427666174234162 1107 12-13 -0.3144360179652139 1107 14-15 -1.0399214885307444 1107 16-17 -0.2692498324796446 1107 18-19 -0.19052479809636935 1107 20-21 -0.36178752593902175 1107 22-23 -0.320532981999861 1107 24-25 -0.06735814154354358 1107 26-27 -1.0628014061167192 1107 28-29 0.18440521382781583 1107 30-31 0.26766315910496274 1107 32-33 0.1933674905551328 1107 34-35 -0.3950216516391052 1107 36-37 -0.1300598187924109 1107 38-39 -0.4686877786702013 1107 40-41 -0.28720058926434433 1107 42-43 0.11493614815373832 1107 44-45 0.1981702515654007 1107 46-47 -0.4030816085947606 1107 48-49 -0.8585035687325444 1107 50-51 -0.2481503095850499 1107 52-53 -0.039344942178992426 1107 54-55 -1.0373259012453744 1107 56-57 -0.7615102972739365 1107 58-59 -1.5293671676526657 1107 60-61 -1.7550291040902195 1107 62-63 -0.8146397314507894 1107 64-65 -0.6963732592234848 1107 66-67 -0.8094733835972399 1107 68-69 -0.41800011858540387 1107 70-71 -1.875637549340734 1107 72-73 -1.8964445720215224 1107 74-75 -1.9434635305664791 1107 76-77 -0.9560789091604676 1107 78-79 -1.0972187907135051 1107 80-81 -1.001913894606357 1107 82-83 -0.7311736327454632 1107 84-85 -0.783945928205771 1107 86-87 -0.39566792039475374 1107 88-89 -0.7085118308291349 1107 90-91 -0.4996180670005934 1107 92-93 -0.6725510141301179 1107 94-95 -0.9736285787423036 1107 96-97 -0.9992096039429761 1107 98-99 -1.8721820146615613 1107 100-101 -2.1315953545214334 1108 1 -0.05935275129945694 1108 2 0.06642084264492354 1108 3 -0.18200567001976964 1108 4 -0.22875011598979 1108 5 -0.3636773053743667 1108 6 0.05669299528668148 1108 7 -0.1353942305477318 1108 8 0.19957291445290082 1108 9 0.4120728486180383 1108 10-11 -0.11510999507567732 1108 12-13 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sequence quality scores pass #Quality Count 5 2.0 6 3.0 7 5.0 8 17.0 9 46.0 10 90.0 11 122.0 12 225.0 13 324.0 14 475.0 15 673.0 16 1051.0 17 1551.0 18 1373.0 19 766.0 20 650.0 21 566.0 22 641.0 23 608.0 24 660.0 25 733.0 26 766.0 27 782.0 28 822.0 29 993.0 30 1176.0 31 1477.0 32 1966.0 33 2590.0 34 4066.0 35 6294.0 36 10746.0 37 18877.0 38 18634.0 39 2361.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03518347059111 21.22459919107256 15.741191949710053 19.999025388626286 2 15.494758373817438 22.387405486357164 40.299034469323395 21.818801670502 3 19.289914892062683 26.905796836760786 31.32191255433393 22.482375716842608 4 12.497108278238423 18.99891636531882 38.061146217627936 30.44282913881482 5 12.443069728926666 39.0194598017487 34.03638666309457 14.501083806230058 6 29.42859577991258 38.523821699480095 17.46478187286165 14.582800647745675 7 26.625756413534475 35.65523371199669 20.805785878657268 16.91322399581157 8 26.640367218224544 34.94295698335586 19.66370797871693 18.752967819702672 9 26.93258331202591 17.055679341539733 21.18323166648403 34.828505679950325 10-11 24.28011347724976 28.542206962048432 26.97763329315362 20.200046267548185 12-13 25.864210308424756 26.46997942211453 28.068722831711863 19.597087437748854 14-15 22.46532977803753 27.2942007281051 27.658862061828053 22.58160743202932 16-17 20.025325394796116 31.158149785099415 28.05944162374743 20.75708319635704 18-19 19.96079434074832 28.210419938878132 32.48529787778062 19.34348784259293 20-21 21.115047911263712 27.282633841058797 32.26126553919958 19.341052708477918 22-23 20.793128009691895 27.741825509402716 32.02342613280085 19.44162034810454 24-25 20.799089259840986 27.841497120453905 32.12428924523018 19.235124374474925 26-27 20.27468814494183 28.189627356796276 32.3781345314412 19.157549966820692 28-29 19.87702422987946 28.78485328138317 32.09971995616705 19.238402532570316 30-31 19.833558583238972 28.56960222084231 32.04027711826229 19.556562077656427 32-33 19.906612606689315 28.754672413583176 31.833290718486317 19.50542426124119 34-35 20.157431420535485 28.625001521958822 32.030536581802245 19.187030475703448 36-37 20.65419877999781 28.204940887119356 31.795546139703646 19.34531419317919 38-39 20.044197684187456 28.854512912298645 31.8284204502563 19.272868953257603 40-41 19.72884781629348 29.336060683542147 31.46497668359085 19.47011481657352 42-43 20.25255569559367 29.481676317119355 30.692701578777527 19.573066408509447 44-45 20.36015633561018 29.342148518829674 30.39412645651459 19.90356868904555 46-47 20.370505655598983 29.917448953501115 29.938756377007465 19.77328901389244 48-49 20.69742241053926 29.87848680766093 29.263615443620555 20.16047533817925 50-51 20.389986728519073 30.030682689849144 29.325711363553346 20.253619218078438 52-53 20.32728202505753 30.342988640099357 28.813724415872205 20.51600491897091 54-55 20.111163872350268 30.319854866006747 28.781458888848306 20.78752237279468 56-57 20.344936747391362 30.017289452216584 29.04932364149956 20.588450158892503 58-59 20.5561846318686 29.335451900013393 28.855121695827396 21.25324177229061 60-61 19.768418745662416 29.044453373269537 30.103736713299483 21.083391167768564 62-63 19.677709999878243 28.341308397559995 30.571891246910422 21.409090355651337 64-65 19.182160207473427 28.364442171652605 30.8665424748268 21.586855146047167 66-67 19.203467630979777 27.934641000353093 30.513448028150155 22.34844334051698 68-69 18.99465488061755 27.733742435864656 30.343597423628104 22.928005259889687 70-71 18.94534341478857 27.9139423603755 30.30098257661541 22.839731648220525 72-73 18.465013210602574 27.289939243403833 30.78496548197392 23.460082064019677 74-75 17.990162058175354 27.170008888239522 30.201750861428696 24.63807819215643 76-77 17.89214791004615 26.424249065517287 30.68634255031596 24.997260474120612 78-79 18.202018726181343 26.738381366353753 30.35090282597314 24.708697081491763 80-81 17.5183548233919 26.13751202347469 29.77864630894547 26.56548684418794 82-83 17.20483130608418 25.641962231069876 30.16644141676103 26.986765046084916 84-85 16.82799430178617 25.63222169460983 30.479356150539992 27.060427853064006 86-87 17.02463138157334 25.446542718340215 29.4864302151441 28.04239568494235 88-89 15.789409601733814 25.095274622249818 29.42555186226881 29.68976391374755 90-91 16.049292516012763 25.301380872360635 30.029711892063027 28.619614719563575 92-93 15.328604913092448 26.364494231530244 29.024991628869746 29.281909226507562 94-95 14.450694622006308 27.453093229109594 27.25280344814991 30.843408700734194 96-97 15.283510489340198 28.9312196369215 26.11559581643959 29.669674057298707 98-99 15.502861317423596 29.535492511871425 24.773529769877022 30.18811640082796 100-101 14.886841736737317 26.884601099617022 23.71361247937457 34.51494468427109 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 49.0 1 32.5 2 17.5 3 21.5 4 23.0 5 25.0 6 40.5 7 62.0 8 86.0 9 124.5 10 138.0 11 146.0 12 164.5 13 175.0 14 192.5 15 221.5 16 246.5 17 255.5 18 274.0 19 294.0 20 308.5 21 346.0 22 405.5 23 463.0 24 503.5 25 588.5 26 703.5 27 795.5 28 935.5 29 1082.5 30 1247.0 31 1440.0 32 1603.0 33 1747.5 34 1876.0 35 2013.0 36 2223.0 37 2426.5 38 2508.0 39 2577.5 40 2702.5 41 2816.5 42 2890.0 43 3012.0 44 3178.0 45 3225.0 46 3191.5 47 3315.0 48 3422.5 49 3221.0 50 2999.0 51 2847.0 52 2647.0 53 2422.0 54 2180.5 55 1931.0 56 1670.0 57 1429.5 58 1194.5 59 968.0 60 723.5 61 537.0 62 410.0 63 271.5 64 172.5 65 117.5 66 79.5 67 56.0 68 42.5 69 30.5 70 21.0 71 12.0 72 9.0 73 8.0 74 5.5 75 4.0 76 3.0 77 2.5 78 2.0 79 2.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05722565170276753 2 0.0 3 0.0 4 0.0 5 0.015828371747573997 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004870268230022769 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.087835287528461E-4 24-25 0.0 26-27 0.0018263505862585383 28-29 0.0012175670575056922 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012784454103809769 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.01095810351755123 92-93 0.004261484701269923 94-95 0.0 96-97 0.0 98-99 0.0012175670575056922 100-101 0.014002021161315461 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 82131.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.71046255372515 #Duplication Level Percentage of deduplicated Percentage of total 1 90.79689003620145 72.37462103225334 2 5.746406586524508 9.160974540672829 3 1.509157285349871 3.6088687584468717 4 0.6766767989979684 2.157528825900086 5 0.352849527242733 1.4062899514190743 6 0.2291230696381383 1.095810351755123 7 0.13441886752104112 0.7500213074235064 8 0.10539661203354363 0.6720970157431421 9 0.0840117922006507 0.6026956934653176 >10 0.34368460445720744 4.803302041859955 >50 0.010692409916446455 0.5917375899477664 >100 0.007637435654604611 1.2382656974832889 >500 0.003054974261841844 1.5377871936296892 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 676 0.823075330873848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 587 0.7147118627558412 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 373 0.4541525124496231 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 212 0.25812421619120673 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 196 0.23864314327111563 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 135 0.16437155276326842 No Hit GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC 101 0.12297427280807491 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 97 0.11810400457805212 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 95 0.11566887046304075 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0012175670575056922 0.0 20-21 0.0 0.0 0.0 0.0012175670575056922 0.0 22-23 0.0 0.0 0.0 0.0018263505862585383 0.0 24-25 0.0 0.0 0.0 0.004870268230022769 0.0 26-27 0.0 0.0 0.0 0.005479051758775615 0.0 28-29 0.0 0.0 0.0 0.018872289391338226 0.0 30-31 0.0 0.0 0.0 0.03348309408140653 0.0 32-33 0.0 0.0 0.0 0.05235538347274476 0.0 34-35 0.0 0.0 0.0 0.06940132227782445 0.0 36-37 0.0 0.0 0.0 0.09009996225542122 0.0 38-39 0.0 0.0 0.0 0.12723575750934482 0.0 40-41 0.0 0.0 0.0 0.2325553079835872 0.0 42-43 0.0012175670575056922 0.0 0.0 0.4017971289768784 0.0 44-45 0.0024351341150113844 0.0 0.0 0.5704301664414169 0.0 46-47 0.0024351341150113844 0.0 0.0 1.0154509259597473 0.0 48-49 0.0024351341150113844 0.0 0.0 1.4233358902241542 0.0 50-51 0.0024351341150113844 0.0 0.0 1.71981346872679 0.0 52-53 0.0024351341150113844 0.0 0.0 2.130742350634961 0.0 54-55 0.0024351341150113844 0.0 0.0 2.5215813760942885 0.0 56-57 0.0024351341150113844 0.0 0.0 3.6216532125506813 0.0 58-59 0.0024351341150113844 0.0 0.0 4.818521630078776 0.0 60-61 0.0024351341150113844 0.0 0.0 6.154192692162521 0.0 62-63 0.0024351341150113844 0.0 0.0 7.822259560945319 0.0 64-65 0.0024351341150113844 0.0 0.0 9.425795375680316 0.0 66-67 0.0024351341150113844 0.0 0.0 11.327635119504208 0.0 68-69 0.0024351341150113844 0.0 0.0 13.280612679743337 0.0 70-71 0.0030439176437642304 0.0 0.0 15.194628094142285 0.0 72-73 0.0036527011725170765 0.0 0.0 17.175000913175296 0.0 74-75 0.0036527011725170765 0.0 0.0 19.366012833156788 0.0 76-77 0.0036527011725170765 0.0 0.0 22.226077851237655 0.0 78-79 0.0036527011725170765 0.0 0.0 24.42013368886291 0.0 80-81 0.0036527011725170765 0.0 0.0 27.156006867078204 0.0 82-83 0.0036527011725170765 0.0 0.0 30.537799369300267 0.0 84-85 0.0036527011725170765 0.0 0.0 34.6957908706822 0.0 86-87 0.0036527011725170765 0.0 0.0 37.98261314241882 0.0 88-89 0.0036527011725170765 0.0 0.0 41.57382717853186 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 40 7.4326344E-6 59.443214 5 AGATTGA 25 0.005006124 56.995987 6 TAGGATT 35 2.7736503E-4 54.281883 7 AGGTAAG 45 1.4933592E-5 52.838413 2 GGTAAGG 40 5.32853E-4 47.554573 3 GTAAGGT 50 2.7849424E-5 47.554573 4 AAGGTGT 50 2.805106E-5 47.496655 6 GGTATCA 505 0.0 43.343464 1 CTATACT 45 9.5188466E-4 42.27073 4 TAGCCAG 45 9.5188466E-4 42.27073 5 CCCTGAG 55 0.002566643 34.54302 7 GTATCAA 785 0.0 33.94498 1 AAGGTAA 80 4.4120522E-4 29.739742 1 GGTGTGC 65 0.0058149337 29.228708 8 ATAGGAT 65 0.0058149337 29.228708 6 GTGTGCA 65 0.0058149337 29.228708 9 TATCAAC 915 0.0 29.104439 2 GTGCACT 50 4.9300135E-5 28.497993 10-11 ATCAACG 970 0.0 27.944439 3 TACAGAG 70 0.008300374 27.174042 5 >>END_MODULE