##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937309_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 104812 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.977445330687328 33.0 31.0 34.0 31.0 34.0 2 32.084207915124225 33.0 31.0 34.0 30.0 34.0 3 32.11605541350227 34.0 31.0 34.0 30.0 34.0 4 35.75623974354082 37.0 35.0 37.0 35.0 37.0 5 35.700272869518756 37.0 35.0 37.0 35.0 37.0 6 35.80387741861619 37.0 35.0 37.0 35.0 37.0 7 35.769902301263215 37.0 35.0 37.0 35.0 37.0 8 35.764931496393544 37.0 35.0 37.0 35.0 37.0 9 37.424979964126244 39.0 37.0 39.0 35.0 39.0 10-11 37.36389917185055 39.0 37.0 39.0 35.0 39.0 12-13 37.27608956989658 39.0 37.0 39.0 35.0 39.0 14-15 38.449333091630734 40.0 38.0 41.0 34.0 41.0 16-17 38.40998645193299 40.0 38.0 41.0 34.0 41.0 18-19 38.42979334427356 40.0 38.0 41.0 34.0 41.0 20-21 38.392092508491395 40.0 38.0 41.0 34.0 41.0 22-23 38.28766744265924 40.0 38.0 41.0 34.0 41.0 24-25 38.15244914704424 40.0 38.0 41.0 34.0 41.0 26-27 37.976901499828266 40.0 38.0 41.0 33.5 41.0 28-29 37.77479677899477 40.0 37.5 41.0 33.0 41.0 30-31 37.64765484868145 40.0 37.0 41.0 33.0 41.0 32-33 37.386792542838606 40.0 36.5 41.0 33.0 41.0 34-35 37.13086287829638 39.5 36.0 41.0 33.0 41.0 36-37 37.074118421554786 40.0 36.0 41.0 32.5 41.0 38-39 36.97467370148456 40.0 36.0 41.0 32.0 41.0 40-41 36.73719612258138 40.0 35.5 41.0 31.5 41.0 42-43 36.376521772316146 40.0 35.0 41.0 30.5 41.0 44-45 35.95211903217189 39.0 35.0 41.0 29.5 41.0 46-47 35.38754627332748 39.0 35.0 41.0 26.0 41.0 48-49 34.85686276380567 39.0 35.0 41.0 22.5 41.0 50-51 33.83342556195855 38.0 34.0 40.0 19.0 40.5 52-53 33.87763805671106 38.5 34.5 40.0 15.5 40.5 54-55 34.14703469068427 39.0 35.0 40.0 10.0 41.0 56-57 33.914613784681144 39.0 34.5 40.5 6.0 41.0 58-59 33.629388810441554 38.0 34.0 40.0 2.0 41.0 60-61 33.306973438155936 38.0 34.0 40.0 2.0 41.0 62-63 32.98855569972904 38.0 34.0 40.0 2.0 41.0 64-65 32.600188909666834 37.5 33.0 40.0 2.0 41.0 66-67 32.18919112315383 37.0 33.0 40.0 2.0 41.0 68-69 31.69335095218105 36.0 32.0 39.0 2.0 41.0 70-71 31.263610082814942 36.0 32.0 39.0 2.0 41.0 72-73 30.83964145326871 35.0 31.0 39.0 2.0 40.0 74-75 30.388633935045604 35.0 31.0 38.0 2.0 40.0 76-77 29.932908445597832 35.0 30.5 37.0 2.0 39.0 78-79 29.469335572262718 35.0 30.0 37.0 2.0 39.0 80-81 29.04259054306759 35.0 29.0 36.0 2.0 39.0 82-83 28.607683280540396 34.0 29.0 36.0 2.0 37.5 84-85 28.17660191581117 34.0 29.0 35.0 2.0 37.0 86-87 27.738899171850548 34.0 27.5 35.0 2.0 36.5 88-89 27.340848376140137 34.0 26.5 35.0 2.0 36.0 90-91 26.90790653741938 34.0 25.5 35.0 2.0 36.0 92-93 26.459446437430827 34.0 25.0 35.0 2.0 35.0 94-95 25.94191504789528 33.5 23.5 35.0 2.0 35.0 96-97 25.34274701370072 33.0 19.0 35.0 2.0 35.0 98-99 24.627289814143417 32.5 4.5 35.0 2.0 35.0 100-101 22.935179177956723 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.044118509620652446 1101 2 0.27399826477984845 1101 3 0.20213461844410574 1101 4 0.002762903644999426 1101 5 0.08634050366910628 1101 6 0.1750557828890038 1101 7 0.02140530756125969 1101 8 0.20185231807528936 1101 9 -0.07140815078631846 1101 10-11 -6.540433409085722E-4 1101 12-13 0.20922323794037823 1101 14-15 0.18431386832627794 1101 16-17 0.10553335574274314 1101 18-19 -0.1965500105549225 1101 20-21 0.020228545709656487 1101 22-23 -0.32876258711664974 1101 24-25 -0.34849303463876424 1101 26-27 0.25034625799019494 1101 28-29 0.567685313996904 1101 30-31 0.17925263021204074 1101 32-33 -0.05566866155651695 1101 34-35 0.5668880934197418 1101 36-37 0.9990038054784023 1101 38-39 0.7195174265347859 1101 40-41 0.46425322823463233 1101 42-43 1.0401379022610513 1101 44-45 0.2817063678995524 1101 46-47 0.5011687477272275 1101 48-49 0.9556171134503657 1101 50-51 1.1341354617461477 1101 52-53 1.1499797819122008 1101 54-55 1.349099881889245 1101 56-57 1.351636074660199 1101 58-59 1.7056180256513827 1101 60-61 1.331828936625996 1101 62-63 1.619151301463809 1101 64-65 1.606026523844399 1101 66-67 1.643919190512019 1101 68-69 1.795395306481236 1101 70-71 1.4724981681386815 1101 72-73 1.2846412895132957 1101 74-75 1.211809053034056 1101 76-77 1.9132841804577971 1101 78-79 2.3365016575134945 1101 80-81 2.2593190961749094 1101 82-83 1.5980151032412486 1101 84-85 1.652885778991923 1101 86-87 1.286046364932183 1101 88-89 0.9208128614738094 1101 90-91 1.1033710242050674 1101 92-93 0.7800483879759597 1101 94-95 0.6501251373288177 1101 96-97 1.0027842039775159 1101 98-99 1.271416127824228 1101 100-101 1.3655242227760418 1102 1 -0.24117733315006262 1102 2 0.0012041471327890463 1102 3 -0.7170749403794261 1102 4 -0.6838179787079426 1102 5 -0.43626979044854153 1102 6 -0.46942951122863974 1102 7 -0.5090174865563881 1102 8 -0.07342341721882661 1102 9 -0.1862059449039677 1102 10-11 -0.01972573451737958 1102 12-13 0.007890517352144855 1102 14-15 -0.3885721610854844 1102 16-17 0.09165467927215332 1102 18-19 0.32684153356272105 1102 20-21 0.2850263398273043 1102 22-23 0.3693991775892371 1102 24-25 0.16676431830241256 1102 26-27 0.31463853740195447 1102 28-29 0.6626761228204288 1102 30-31 0.7872489537414467 1102 32-33 0.28481847079642364 1102 34-35 0.31426860812562296 1102 36-37 0.5642152025372269 1102 38-39 0.3262729412406671 1102 40-41 0.8182513899993396 1102 42-43 0.8632537110845817 1102 44-45 1.1848313678995552 1102 46-47 0.48016690949193475 1102 48-49 0.8442200546268381 1102 50-51 1.0436942852755564 1102 52-53 0.8281966936769081 1102 54-55 1.3514895877715958 1102 56-57 1.3179963687778482 1102 58-59 1.570323908004326 1102 60-61 1.4822884954495237 1102 62-63 1.4035722573461626 1102 64-65 1.3374603473738134 1102 66-67 1.0916673522767262 1102 68-69 0.997601188834178 1102 70-71 0.8497500063739771 1102 72-73 0.8204868777485927 1102 74-75 0.5931969206811125 1102 76-77 1.1745433716342681 1102 78-79 1.1216579075134945 1102 80-81 1.2368926255866732 1102 82-83 1.7144673091236022 1102 84-85 1.072462984874278 1102 86-87 1.3132522472851242 1102 88-89 1.141584920297337 1102 90-91 1.1710180830285992 1102 92-93 1.115342505623019 1102 94-95 0.9458971961523481 1102 96-97 1.0632161892716319 1102 98-99 1.2704510542948135 1102 100-101 1.1219120904230984 1103 1 0.3421560001832731 1103 2 -0.08280122921129873 1103 3 0.1640574520936937 1103 4 -0.25656663462191887 1103 5 -0.34705608077112515 1103 6 -0.07124402735767177 1103 7 0.351449583336084 1103 8 -0.05905849786398676 1103 9 0.2607866626229125 1103 10-11 0.4180682842998209 1103 12-13 0.07664891788977712 1103 14-15 0.3426946399897801 1103 16-17 0.31065669540118535 1103 18-19 0.9986745308745455 1103 20-21 0.3971735172466637 1103 22-23 0.3710792851161173 1103 24-25 -0.19798702578361116 1103 26-27 -0.09996999754428515 1103 28-29 -0.11735579922257955 1103 30-31 -0.0016455355058653254 1103 32-33 -0.1544036394186321 1103 34-35 0.08984824522239165 1103 36-37 0.475379517053355 1103 38-39 -0.08547941090987621 1103 40-41 -0.38802381161355726 1103 42-43 0.2792903374286624 1103 44-45 0.027405292630739098 1103 46-47 0.8370637508897829 1103 48-49 1.2121636030139342 1103 50-51 1.5940975110820048 1103 52-53 1.5020038173328203 1103 54-55 1.517316200674827 1103 56-57 1.52750577738 1103 58-59 1.7934421875742217 1103 60-61 2.008078145987156 1103 62-63 1.2373676202493868 1103 64-65 1.6057735194168217 1103 66-67 1.0093840861476941 1103 68-69 0.4445097909847142 1103 70-71 0.003269831642793264 1103 72-73 -0.2620064018212993 1103 74-75 -0.02117639921135961 1103 76-77 0.16391669152674027 1103 78-79 0.36069520590059057 1103 80-81 -0.6216423206498867 1103 82-83 -0.56871481453231 1103 84-85 -0.28750005276013013 1103 86-87 -0.044946677446059624 1103 88-89 -0.08573362808975915 1103 90-91 -0.29672385245527266 1103 92-93 -0.4545499674952609 1103 94-95 -0.9091095242777563 1103 96-97 -1.31426196933052 1103 98-99 -1.4218792548449706 1103 100-101 -1.4266010144156134 1104 1 0.19303319316572498 1104 2 0.18870414713278905 1104 3 0.16080334909425886 1104 4 -0.0645745576553125 1104 5 0.16652626218304079 1104 6 -0.0894953007023247 1104 7 0.07527527660150923 1104 8 0.13915881962327603 1104 9 0.02719866035918983 1104 10-11 0.3077824891668328 1104 12-13 -0.05563744317417019 1104 14-15 0.16364823365135805 1104 16-17 0.16443428453531084 1104 18-19 0.10089580987851576 1104 20-21 -0.3889045812253258 1104 22-23 -0.10592976977918767 1104 24-25 0.2810735288287276 1104 26-27 0.10390662950721463 1104 28-29 0.19454289913622347 1104 30-31 0.2342061905835564 1104 32-33 -0.0028048844667338813 1104 34-35 0.2581419633887805 1104 36-37 0.7523319788530216 1104 38-39 0.8186660333459272 1104 40-41 0.16015106105196963 1104 42-43 0.06041654003195163 1104 44-45 -0.6169778426267598 1104 46-47 -1.283196577350168 1104 48-49 -0.5225562611626344 1104 50-51 -0.9990688726191834 1104 52-53 -0.35149080632309193 1104 54-55 -0.19703014907051397 1104 56-57 -0.5693391575379465 1104 58-59 -0.627044513048304 1104 60-61 -0.5057871624452162 1104 62-63 -0.3519787294959471 1104 64-65 -0.5153357052577654 1104 66-67 -0.19307771351274639 1104 68-69 0.1390485572552329 1104 70-71 0.2660740195318745 1104 72-73 0.14532240406438035 1104 74-75 0.33394527594427004 1104 76-77 0.5653739637395319 1104 78-79 0.8036069206713883 1104 80-81 0.8470899940077281 1104 82-83 0.7979377038604447 1104 84-85 0.7093873269795417 1104 86-87 1.0336469841272269 1104 88-89 0.885828341349967 1104 90-91 1.3289128198707019 1104 92-93 0.9969214529914403 1104 94-95 0.8151406172049782 1104 96-97 1.8878461234821593 1104 98-99 1.9454099358737587 1104 100-101 1.0995025509494134 1105 1 0.3004893335166017 1105 2 0.5845374804661247 1105 3 0.5094875596205739 1105 4 0.4463903546253931 1105 5 0.7147718762181228 1105 6 0.45765382210469596 1105 7 0.4623366801102762 1105 8 0.6270974161145091 1105 9 0.8528565550960323 1105 10-11 0.5857430154826204 1105 12-13 0.6784634340188092 1105 14-15 0.473407005581187 1105 16-17 1.0838421792721533 1105 18-19 0.9375186168960497 1105 20-21 0.9647138398273043 1105 22-23 0.8277325109225657 1105 24-25 0.6511393183024126 1105 26-27 0.9695864540686259 1105 28-29 1.3332490394871002 1105 30-31 1.617978120408118 1105 32-33 1.869453887463088 1105 34-35 0.7504665247922944 1105 36-37 0.8181214525372269 1105 38-39 0.7364291912406671 1105 40-41 1.098199306666011 1105 42-43 1.4244516277512531 1105 44-45 1.2942063678995552 1105 46-47 1.5205314928252704 1105 48-49 2.344220054626838 1105 50-51 2.179110951942228 1105 52-53 2.7318425270102367 1105 54-55 3.054614587771596 1105 56-57 2.833621368777848 1105 58-59 3.0286572413376547 1105 60-61 2.9953093287828594 1105 62-63 2.0142493406794912 1105 64-65 2.342668680707142 1105 66-67 2.9966152689433976 1105 68-69 2.747601188834178 1105 70-71 2.173968756373977 1105 72-73 1.7944452110819284 1105 74-75 1.2116865040144482 1105 76-77 1.2331371216342681 1105 78-79 1.3677516575134945 1105 80-81 2.1848092922533375 1105 82-83 2.0868631424569344 1105 84-85 1.3979838182076136 1105 86-87 2.1153355806184564 1105 88-89 2.771793253630669 1105 90-91 2.3481014163619314 1105 92-93 2.2507591722896834 1105 94-95 2.492772196152348 1105 96-97 2.410872439271632 1105 98-99 1.0243573042948135 1105 100-101 0.33415167375643406 1106 1 0.35092793000783473 1106 2 0.38607256818541913 1106 3 0.30554019119951903 1106 4 0.422267547607845 1106 5 -0.04400005360643888 1106 6 0.44997838350820274 1106 7 0.10159106607519419 1106 8 -0.08452539090303901 1106 9 0.5666723445697173 1106 10-11 -0.1856385634647495 1106 12-13 0.16804676735214485 1106 14-15 0.6965429704934607 1106 16-17 0.4999606003247834 1106 18-19 0.6469484414574609 1106 20-21 0.15056910298519455 1106 22-23 0.6901228617997646 1106 24-25 0.8337051077760975 1106 26-27 0.7749592610861598 1106 28-29 0.45112184650464116 1106 30-31 0.14210092742565195 1106 32-33 0.18798458921747851 1106 34-35 0.33051038444141767 1106 36-37 -0.8463522316733005 1106 38-39 -0.8458076508645931 1106 40-41 -0.14905946526381797 1106 42-43 0.2577849610845817 1106 44-45 -0.8472410005214996 1106 46-47 -0.8753018405080653 1106 48-49 -0.5225562611626344 1106 50-51 -1.0714372936718135 1106 52-53 -0.5159644905336194 1106 54-55 -0.38124067538629447 1106 56-57 0.08197663193573845 1106 58-59 -0.877044513048304 1106 60-61 -1.1636818992873188 1106 62-63 -1.3190839926538374 1106 64-65 -0.7258620210472415 1106 66-67 -0.11413034509169506 1106 68-69 -0.37410933748160957 1106 70-71 -0.8589259804681291 1106 72-73 0.13216450932753787 1106 74-75 -0.0476336714241512 1106 76-77 0.17721606900268938 1106 78-79 0.15229113119770687 1106 80-81 0.136563678218252 1106 82-83 -0.02443071719218537 1106 84-85 -0.0011389888099344603 1106 86-87 0.2770680367588092 1106 88-89 -0.587855869176348 1106 90-91 -0.28950823276087334 1106 92-93 0.4969214529914403 1106 94-95 0.19671956457339945 1106 96-97 0.4338987550611044 1106 98-99 -0.43616901149465903 1106 100-101 -0.10444481747164147 1107 1 0.4190790771063462 1107 2 0.14383235226099345 1107 3 0.422949098082114 1107 4 -0.3004045171694827 1107 5 0.3237462351924876 1107 6 0.007333309284177858 1107 7 0.3870161672897652 1107 8 0.4107512622683558 1107 9 0.3095873243267988 1107 10-11 -0.3958274973378906 1107 12-13 0.38599548530086025 1107 14-15 0.038310851735026574 1107 16-17 0.4243870510670291 1107 18-19 0.7211724630499035 1107 20-21 0.8276946090580708 1107 22-23 0.6762902032302591 1107 24-25 0.9099534208665148 1107 26-27 0.36942619765836326 1107 28-29 0.6737939112819618 1107 30-31 0.5699011973311912 1107 32-33 0.5705756823348835 1107 34-35 1.0886075504333164 1107 36-37 0.0841470935628621 1107 38-39 0.5104676527791341 1107 40-41 0.9531672553839527 1107 42-43 1.1680413713409905 1107 44-45 -0.31957568338249587 1107 46-47 -0.38171209691832075 1107 48-49 -0.07485045819367286 1107 50-51 -0.711113407032137 1107 52-53 -1.6303369601692452 1107 54-55 -1.1096482327412218 1107 56-57 -0.3691029901965095 1107 58-59 -0.7053171176366959 1107 60-61 -0.4261650301914983 1107 62-63 -1.9793404029102462 1107 64-65 -2.4802479859595223 1107 66-67 -4.373577038748916 1107 68-69 -3.5745141957812088 1107 70-71 -3.0471850897798696 1107 72-73 -2.9811958145591007 1107 74-75 -2.095204521626581 1107 76-77 -2.056926980929834 1107 78-79 -1.5288829578711187 1107 80-81 -1.9562163487723012 1107 82-83 -2.0902201908763978 1107 84-85 -1.9545802843564886 1107 86-87 -1.5184785219456458 1107 88-89 -1.0679503361129186 1107 90-91 -1.0212896092790942 1107 92-93 -0.8590164687359554 1107 94-95 -0.48479190641175407 1107 96-97 -1.0714993556001637 1107 98-99 -0.7833350033974931 1107 100-101 -1.723540633935876 1108 1 0.4205873727322924 1108 2 0.3651747353680861 1108 3 0.17860520667939994 1108 4 -0.011942978707942586 1108 5 0.29810520955145847 1108 6 0.5397616652419472 1108 7 0.6743464840318438 1108 8 0.4665582004282314 1108 9 0.06976831980191633 1108 10-11 0.37434595665909143 1108 12-13 0.4768702967639129 1108 14-15 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0.4998874842851464 2316 80-81 0.9315284523583252 2316 82-83 0.9176538248716319 2316 84-85 1.0863040281813667 2316 86-87 0.7507522472851242 2316 88-89 1.1609008651792259 2316 90-91 0.3599944609813548 2316 92-93 0.7255787260954598 2316 94-95 0.6610792827665222 2316 96-97 1.1721913369094281 2316 98-99 0.6660895877593802 2316 100-101 0.7232592853049873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 421.0 3 87.0 4 74.0 5 80.0 6 68.0 7 67.0 8 114.0 9 130.0 10 208.0 11 264.0 12 405.0 13 585.0 14 747.0 15 994.0 16 1532.0 17 2061.0 18 1758.0 19 1002.0 20 881.0 21 906.0 22 925.0 23 1002.0 24 1036.0 25 1100.0 26 1114.0 27 1154.0 28 1303.0 29 1501.0 30 1826.0 31 2304.0 32 2966.0 33 3952.0 34 5802.0 35 8635.0 36 13565.0 37 20647.0 38 20021.0 39 3575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28570882105424 19.892318873842857 15.816502180399358 21.005470124703542 2 14.885215179706849 20.972969871972616 41.40476349833153 22.737051449989004 3 18.28082420997275 26.462685853611895 31.56475594014438 23.691733996270976 4 11.67449109353935 17.46010498436708 39.13392677866274 31.731477143430826 5 12.180173633686465 37.4373733124259 35.49642406394615 14.886028989941485 6 28.758744600328757 37.82350242746283 18.483122443518482 14.934630528689935 7 25.416941441828904 35.40346551213312 21.917022679702956 17.262570366335027 8 25.225776240216362 33.9710815279198 21.214843414023452 19.588298817840386 9 25.82377675840978 16.630351681957187 21.668577981651378 35.87729357798165 10-11 23.367183579842777 27.506152780100834 28.04903108647344 21.077632553582948 12-13 25.217195997285646 25.65875617658584 28.93891750853014 20.18513031759837 14-15 21.844298329446847 26.122462632363625 28.95896249856646 23.074276539623074 16-17 19.406002102647424 30.122335850138583 29.068144891522508 21.403517155691485 18-19 19.35055378763988 27.37736876809724 33.456609615550015 19.815467828712862 20-21 20.379024627999655 26.044802507717158 33.81308714890526 19.763085715377926 22-23 19.801606452568556 26.596776582680704 33.76321787453232 19.838399090218413 24-25 19.556678962558056 26.635576537145695 33.88553353338048 19.92221096691577 26-27 19.377652050919377 26.85213502045185 33.84160327229634 19.92860965633243 28-29 19.084888621312462 27.183375858872548 33.90049979453953 19.831235725275462 30-31 19.00978082728502 27.012189003617042 33.72784740571559 20.25018276338234 32-33 19.032191784094763 27.318816687930354 33.37490503017532 20.27408649779957 34-35 19.45539421565395 27.433835509749677 33.120708685119624 19.990061589476753 36-37 19.4000611597416 27.41389855127862 33.15001337869348 20.036026910286303 38-39 19.129416823948205 27.91695534797047 33.04966911149867 19.90395871658265 40-41 19.17221739088885 27.881040892193308 33.010005638325325 19.93673607859252 42-43 19.296502099886766 27.994190074391895 32.38651294571829 20.32279488000306 44-45 19.23876424629059 27.921057032948653 32.25957517979595 20.580603540964805 46-47 19.59406191320302 28.046805196542646 31.663266329977592 20.69586656027674 48-49 19.653684617920675 28.45538943757197 31.117258909928374 20.77366703457898 50-51 19.496858351052392 28.463578373987623 31.069116276847364 20.97044699811262 52-53 19.665449889152207 28.578663710725476 30.622181025915452 21.133705374206865 54-55 19.285611888947294 28.29645911979739 30.92894347015817 21.48898552109715 56-57 19.396916255357375 28.262084848369017 30.79778872478989 21.54321017148372 58-59 19.604283031759607 27.580736868761672 31.138940518225823 21.676039581252894 60-61 19.486451748539103 26.976573064040632 31.891308191065942 21.645666996354322 62-63 19.157699585252573 26.681925039659028 32.270503239616986 21.889872135471418 64-65 18.812264380832247 26.50593198878106 32.66583528040174 22.01596834998495 66-67 18.886659021406725 26.1845374617737 32.70307721712538 22.22572629969419 68-69 18.75895877529958 25.793150239856278 32.28933738508877 23.158553599755365 70-71 18.394934894277863 25.863098793453588 32.56337355154701 23.17859276072154 72-73 18.352670209105852 25.406647807637906 32.80610879620781 23.434573187048436 74-75 18.183077863996026 25.49357822713199 32.404151217461106 23.919192691410878 76-77 17.949208017774804 25.19387438182383 32.87144324724658 23.98547435315479 78-79 18.028964456187797 25.060800512205496 32.6210873855063 24.289147646100407 80-81 17.740733308488377 24.922837294192966 32.1608424351881 25.175586962130552 82-83 18.02916778164303 23.54830077602355 32.94802935892045 25.474502083412975 84-85 18.033961795369194 23.674352824256786 32.834672756983004 25.457012623391016 86-87 18.192851102054078 23.38084294116804 32.4925582084275 25.933747748350385 88-89 17.424626116871327 22.792297768646375 32.67762434898944 27.105451765492855 90-91 17.667182692123923 22.93780937637368 33.46616210844179 25.928845823060602 92-93 17.01922295860589 23.7221725813619 32.91173111749275 26.346873342539457 94-95 16.727668886956355 24.534838160950297 31.59373476935427 27.14375818273908 96-97 17.15781829300921 25.41661888927111 31.017085196651763 26.40847762106792 98-99 17.676654259817575 25.345807074292974 30.339762916103265 26.637775749786186 100-101 18.573694671661187 24.64834492775782 29.64844048620136 27.129519914379635 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 131.0 1 81.5 2 40.0 3 50.5 4 36.5 5 34.0 6 68.0 7 130.5 8 213.5 9 276.0 10 269.0 11 235.5 12 228.5 13 242.0 14 284.5 15 324.0 16 339.5 17 347.0 18 374.0 19 419.0 20 452.0 21 473.0 22 549.5 23 613.5 24 672.5 25 802.5 26 952.0 27 1119.5 28 1272.5 29 1424.0 30 1647.5 31 1843.0 32 2035.0 33 2220.5 34 2410.5 35 2663.5 36 2854.5 37 3055.0 38 3193.5 39 3248.5 40 3374.0 41 3528.0 42 3691.5 43 3886.5 44 4052.0 45 4062.0 46 3998.5 47 4113.0 48 4336.5 49 4209.0 50 3785.0 51 3471.5 52 3213.5 53 2979.0 54 2735.5 55 2385.0 56 2028.0 57 1682.0 58 1389.5 59 1126.0 60 836.5 61 639.0 62 508.0 63 365.5 64 235.0 65 167.0 66 126.0 67 91.0 68 65.0 69 47.5 70 33.0 71 20.5 72 17.0 73 12.5 74 9.0 75 8.5 76 8.0 77 4.5 78 1.5 79 4.5 80 4.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23279777124756706 2 0.21467007594550241 3 0.21657825439835135 4 0.21467007594550241 5 0.21371598671907796 6 0.16791970385070412 7 0.1726901499828264 8 0.16505743617143076 9 0.16410334694500628 10-11 0.176029462275312 12-13 0.1745983284356753 14-15 0.16791970385070412 16-17 0.17364423920925084 18-19 0.16124107926573295 20-21 0.16696561462427967 22-23 0.16362630233179407 24-25 0.16219516849215737 26-27 0.16791970385070412 28-29 0.16124107926573295 30-31 0.1607640346525207 32-33 0.16553448078464297 34-35 0.15980994542609625 36-37 0.1602869900393085 38-39 0.16171812387894516 40-41 0.16314925771858185 42-43 0.15599358852039844 44-45 0.1712590161431897 46-47 0.15790176697324734 48-49 0.15980994542609625 50-51 0.16171812387894516 52-53 0.15647063313361065 54-55 0.16887379307712858 56-57 0.1588558561996718 58-59 0.1588558561996718 60-61 0.15980994542609625 62-63 0.16219516849215737 64-65 0.15980994542609625 66-67 0.16410334694500628 68-69 0.15837881158645958 70-71 0.16648857001106743 72-73 0.16791970385070412 74-75 0.1602869900393085 76-77 0.16171812387894516 78-79 0.1588558561996718 80-81 0.1555165439071862 82-83 0.16791970385070412 84-85 0.1574247223600351 86-87 0.1588558561996718 88-89 0.159332900812884 90-91 0.15837881158645958 92-93 0.16362630233179407 94-95 0.16314925771858185 96-97 0.15265427622791283 98-99 0.15790176697324734 100-101 0.15647063313361065 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 104812.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.94218219287868 #Duplication Level Percentage of deduplicated Percentage of total 1 91.077587750182 72.80941113612946 2 5.519817637160009 8.82532534442621 3 1.5061642936423636 3.612181811242988 4 0.626573893947893 2.003587375491356 5 0.37117044003389466 1.4836087470900279 6 0.2315339722397928 1.110559859558066 7 0.13486257145926075 0.754684578101744 8 0.08473665994342933 0.5419226806090905 9 0.07996276360858824 0.5753158035339465 >10 0.3449140101922687 4.53574018242186 >50 0.01074126675339245 0.5762698927603709 >100 0.009547792669682179 1.3796130214097622 >500 0.0011934740837102724 0.7813990764416289 >1k 0.0011934740837102724 1.010380490783498 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1059 1.010380490783498 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 819 0.7813990764416289 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 484 0.46177918558943637 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 244 0.23279777124756706 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 134 0.127847956340877 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 134 0.127847956340877 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 119 0.11353661794451017 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 112 0.10685799335953898 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 111 0.10590390413311453 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 108 0.10304163645384115 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 9.540892264244552E-4 0.0 0.0 0.0 0.0 16-17 9.540892264244552E-4 0.0 0.0 0.0 0.0 18-19 9.540892264244552E-4 0.0 0.0 0.0 0.0 20-21 9.540892264244552E-4 0.0 0.0 0.0 0.0 22-23 9.540892264244552E-4 0.0 0.0 9.540892264244552E-4 0.0 24-25 9.540892264244552E-4 0.0 0.0 0.002862267679273366 0.0 26-27 9.540892264244552E-4 0.0 0.0 0.003816356905697821 0.0 28-29 0.0019081784528489105 0.0 0.0 0.011449070717093463 0.0 30-31 0.0019081784528489105 0.0 0.0 0.027191542953096975 0.0 32-33 0.0019081784528489105 0.0 0.0 0.0415028813494638 0.0 34-35 0.0019081784528489105 0.0 0.0 0.058199442811891766 0.0 36-37 0.0019081784528489105 0.0 0.0 0.07298782582147081 0.0 38-39 0.0019081784528489105 0.0 0.0 0.10494981490669007 0.0 40-41 0.0019081784528489105 0.0 0.0 0.19368011296416443 0.0 42-43 0.002385223066061138 0.0 0.0 0.37495706598481093 0.0 44-45 0.002862267679273366 0.0 0.0 0.5328588329580582 0.0 46-47 0.002862267679273366 0.0 0.0 0.9378697095752394 0.0 48-49 0.002862267679273366 0.0 0.0 1.3323856047017517 0.0 50-51 0.002862267679273366 0.0 0.0 1.5957142311949013 0.0 52-53 0.002862267679273366 0.0 0.0 1.963515627981529 0.0 54-55 0.002862267679273366 0.0 0.0 2.335610426287066 0.0 56-57 0.002862267679273366 0.0 0.0 3.25296721749418 0.0 58-59 0.002862267679273366 0.0 0.0 4.373545013929703 0.0 60-61 0.002862267679273366 0.0 0.0 5.569495859252758 0.0 62-63 0.002862267679273366 0.0 0.0 6.944815479143609 0.0 64-65 0.002862267679273366 0.0 0.0 8.37308705110102 0.0 66-67 0.002862267679273366 0.0 0.0 10.11429988932565 0.0 68-69 0.002862267679273366 0.0 0.0 11.860283173682404 0.0 70-71 0.002862267679273366 0.0 0.0 13.621531885661948 0.0 72-73 0.002862267679273366 0.0 0.0 15.410926229821012 0.0 74-75 0.002862267679273366 0.0 0.0 17.321012861122774 0.0 76-77 0.002862267679273366 0.0 0.0 19.834560928138 0.0 78-79 0.002862267679273366 0.0 0.0 21.797122466893107 0.0 80-81 0.002862267679273366 0.0 0.0 24.214784566652675 0.0 82-83 0.002862267679273366 0.0 0.0 27.121417394954776 0.0 84-85 0.002862267679273366 0.0 0.0 30.756974392245162 0.0 86-87 0.002862267679273366 0.0 0.0 33.80862878296378 0.0 88-89 0.002862267679273366 0.0 0.0 37.34209823302675 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGACA 20 0.0020693254 71.24534 6 GCGACAG 25 0.0050098435 56.99627 7 GAGCGCT 25 0.0050098435 56.99627 9 ACATAGA 35 2.7705368E-4 54.308144 1 GGTATCA 465 0.0 53.14023 1 TAGACAG 45 9.56541E-4 42.23967 5 GTATCAA 825 0.0 39.743687 1 GTATTAA 55 0.0025637483 34.55973 1 GTTATAC 55 0.0025637483 34.55973 1 ATCAACG 960 0.0 33.659737 3 TCAACGC 960 0.0 33.659737 4 CAACGCA 985 0.0 33.287865 5 AACGCAG 990 0.0 33.103893 6 TATCAAC 1005 0.0 33.098248 2 ATGGGAG 125 2.114648E-7 30.41256 5 ACGCAGA 1120 0.0 29.261477 7 ATAGATC 65 0.005808359 29.242847 3 TGGGAGC 65 0.005821971 29.228857 6 CGCAGAG 1130 0.0 29.002525 8 GCAGAGT 1200 0.0 27.310709 9 >>END_MODULE