FastQCFastQC Report
Thu 26 May 2016
SRR937309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937309_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104812
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8230.7852154333473267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.649734763195054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.4226615273060337No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3020.28813494638018544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.22421096820974695No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.1955882914170133No Hit
GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC1330.12689386711445255No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1180.11258252871808572No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.10208754722741671No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGC200.002069629371.242849
AGGGTAG352.7776163E-454.2802625
CTAAGGG352.7776163E-454.2802622
TATATCA405.368316E-447.4952285
AAGGGTA405.368316E-447.4952284
TAAGGGT459.589761E-442.217983
TACTCAC250.001609818238.0143290-91
GTACCTT500.001609916137.9961856
TAGTTTA500.001609916137.9961859
GCTAAGG550.00254626534.608031
ATGGGGA550.002570252634.5419855
GGGTAGT550.002570252634.5419856
GGTATCA7000.030.5910261
CATGGGG1401.6765625E-830.5326464
GGTAGTT650.005823043629.2278337
CTGGACT650.005823043629.2278334
GTATATG856.3009834E-427.9917891
CTCCTGG700.00828389927.1920221
TACATGG5850.025.9802952
GTACATG6000.025.3792231