##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937309_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 104812 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28995725680266 33.0 31.0 34.0 31.0 34.0 2 32.50356829370683 34.0 31.0 34.0 31.0 34.0 3 32.56977254512842 34.0 31.0 34.0 31.0 34.0 4 36.168597107201464 37.0 35.0 37.0 35.0 37.0 5 36.115463878181885 37.0 35.0 37.0 35.0 37.0 6 36.20620730450712 37.0 36.0 37.0 35.0 37.0 7 36.16680341945579 37.0 36.0 37.0 35.0 37.0 8 36.14972522230279 37.0 36.0 37.0 35.0 37.0 9 37.868745945120786 39.0 38.0 39.0 35.0 39.0 10-11 37.80661088424989 39.0 37.5 39.0 35.0 39.0 12-13 37.762240964775025 39.0 37.0 39.0 35.0 39.0 14-15 38.94751078120826 40.0 38.0 41.0 36.0 41.0 16-17 38.948545968018934 40.0 38.0 41.0 36.0 41.0 18-19 38.998616570621685 40.0 38.0 41.0 36.0 41.0 20-21 38.93414399114605 40.0 38.0 41.0 35.0 41.0 22-23 38.824271075831014 40.0 38.0 41.0 35.0 41.0 24-25 38.68546540472465 40.0 38.0 41.0 35.0 41.0 26-27 38.59330992634432 40.0 38.0 41.0 34.5 41.0 28-29 38.489834179292444 40.0 38.0 41.0 34.0 41.0 30-31 38.497924855932524 40.0 38.0 41.0 35.0 41.0 32-33 38.35157233904515 40.0 38.0 41.0 34.5 41.0 34-35 38.180694958592525 40.0 37.5 41.0 34.0 41.0 36-37 37.98654734190741 40.0 37.0 41.0 34.0 41.0 38-39 37.810880433538145 40.0 37.0 41.0 33.5 41.0 40-41 37.570344998664275 40.0 37.0 41.0 33.0 41.0 42-43 37.26342880586192 40.0 37.0 41.0 33.0 41.0 44-45 36.79891615463878 40.0 36.0 41.0 31.5 41.0 46-47 36.236246803801095 40.0 36.0 41.0 30.5 41.0 48-49 35.73668568484525 40.0 35.5 41.0 28.5 41.0 50-51 35.398117581956264 39.0 35.0 41.0 26.5 41.0 52-53 35.107077433881614 39.0 35.0 41.0 25.5 41.0 54-55 34.83669331755905 39.0 35.0 40.5 24.0 41.0 56-57 34.53649868335687 39.0 35.0 40.0 22.5 41.0 58-59 34.243865206274094 38.0 35.0 40.0 21.0 41.0 60-61 33.85988722665344 38.0 34.0 40.0 18.0 41.0 62-63 33.493879517612484 38.0 34.0 40.0 13.0 41.0 64-65 33.133787161775366 37.0 33.5 40.0 7.5 41.0 66-67 32.65972407739572 36.5 33.0 39.5 2.0 41.0 68-69 32.21394019768729 36.0 33.0 39.0 2.0 40.5 70-71 31.77056062282945 35.5 32.0 39.0 2.0 40.0 72-73 31.317396862954624 35.0 32.0 38.0 2.0 40.0 74-75 30.880571881082318 35.0 31.0 37.5 2.0 39.5 76-77 29.58269568370034 34.0 29.5 36.0 2.0 39.0 78-79 30.134302369957638 35.0 31.0 36.0 2.0 39.0 80-81 29.96118287982292 35.0 31.0 36.0 2.0 38.5 82-83 29.669269740106095 35.0 31.0 36.0 2.0 37.0 84-85 29.31545529137885 34.0 30.5 35.0 2.0 37.0 86-87 28.952386177155287 34.0 30.0 35.0 2.0 36.0 88-89 28.605584284242262 34.0 29.5 35.0 2.0 36.0 90-91 28.234810899515324 34.0 29.0 35.0 2.0 35.5 92-93 27.971420257222455 34.0 29.0 35.0 2.0 35.0 94-95 27.642135442506582 34.0 29.0 35.0 2.0 35.0 96-97 27.25348719612258 34.0 27.0 35.0 2.0 35.0 98-99 26.85814601381521 33.5 27.0 35.0 2.0 35.0 100-101 25.22578998587948 32.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1264612764231785 1101 2 0.05125584963753482 1101 3 -0.04811994875944947 1101 4 0.058737010386437305 1101 5 -0.08708038847908028 1101 6 0.03947343837707251 1101 7 0.12311681412098352 1101 8 -0.034034783221336795 1101 9 -0.09296373981806738 1101 10-11 -0.14172395324671072 1101 12-13 -0.18011764827861754 1101 14-15 -0.3629526213328944 1101 16-17 -0.1184172912092194 1101 18-19 -0.13379511743828232 1101 20-21 -0.23133217610578782 1101 22-23 -0.5271066919843577 1101 24-25 -0.330663289486786 1101 26-27 -0.21190797681015994 1101 28-29 -0.33661379138816017 1101 30-31 -0.12800218503980432 1101 32-33 -0.32118849968991725 1101 34-35 -0.055377760372934404 1101 36-37 -0.08956830303287688 1101 38-39 -0.3856096979355641 1101 40-41 -0.012428325534315832 1101 42-43 0.28992193252557286 1101 44-45 0.7457468052714802 1101 46-47 1.0646155443858802 1101 48-49 0.7551557705831229 1101 50-51 0.576654968038703 1101 52-53 0.897150767442966 1101 54-55 0.737562498219205 1101 56-57 1.0550085060499086 1101 58-59 0.8857224450854275 1101 60-61 0.7901180809046409 1101 62-63 0.8878854464220467 1101 64-65 0.727334138640984 1101 66-67 1.3345427543439783 1101 68-69 1.328141330100852 1101 70-71 1.8465992408407246 1101 72-73 1.574281049828727 1101 74-75 1.4370662693918526 1101 76-77 1.4775515818268516 1101 78-79 1.825776820217392 1101 80-81 1.696319743048516 1101 82-83 1.8332648400532854 1101 84-85 2.168557995388653 1101 86-87 2.065480331924171 1101 88-89 2.147579288025536 1101 90-91 2.143448921169206 1101 92-93 2.2731880355825282 1101 94-95 2.1806710337219677 1101 96-97 1.635920548909997 1101 98-99 0.8961813085658399 1101 100-101 1.2394479381887074 1102 1 -0.013796076517998301 1102 2 0.12138452610812323 1102 3 0.12164108065231716 1102 4 -0.13363431314297713 1102 5 0.10795637622680232 1102 6 -0.0846993557405753 1102 7 0.15712416706215748 1102 8 -0.1810936067507498 1102 9 -0.15270638687689342 1102 10-11 -0.3934702767761209 1102 12-13 -0.056542280631553865 1102 14-15 0.04288377572592594 1102 16-17 0.11260292937901539 1102 18-19 0.03656333844406845 1102 20-21 -0.07494430845873268 1102 22-23 -0.09957911845494749 1102 24-25 -0.07422946595737301 1102 26-27 -0.053176359163096265 1102 28-29 0.052356796847135456 1102 30-31 -0.12455549386333331 1102 32-33 -0.7196259996899173 1102 34-35 -0.3818023927258736 1102 36-37 -0.7156252883269971 1102 38-39 -0.5574846979355641 1102 40-41 -0.27759376671078684 1102 42-43 0.15793663840793215 1102 44-45 0.1772265846832397 1102 46-47 -0.456432249731769 1102 48-49 -0.9433276852992307 1102 50-51 -0.5328578996083522 1102 52-53 -0.8218290119688021 1102 54-55 -1.0616562517807893 1102 56-57 -0.6602028910089146 1102 58-59 -0.5277885843263377 1102 60-61 -1.1753230955659504 1102 62-63 -1.1445594065191287 1102 64-65 -0.8050647584178421 1102 66-67 -0.8683065103619043 1102 68-69 -0.4881730081344422 1102 70-71 -0.19549634739456678 1102 72-73 -0.5038899060536295 1102 74-75 -0.28945027472579454 1102 76-77 -0.13940613876138386 1102 78-79 0.09985770257033266 1102 80-81 0.11047415481322176 1102 82-83 0.13156447240622882 1102 84-85 0.4531168189180619 1102 86-87 0.23648217015946216 1102 88-89 0.1909156850843594 1102 90-91 -0.22553085824256058 1102 92-93 -0.11100314088805874 1102 94-95 0.06348353372196769 1102 96-97 0.07695915185117386 1102 98-99 0.4561077791540775 1102 100-101 0.2611391146592972 1103 1 -0.021356560388966273 1103 2 -0.02268469432198117 1103 3 0.15263906452328513 1103 4 0.026731950297886442 1103 5 -0.05014174205276589 1103 6 -0.23800916756853496 1103 7 -0.2247642737980584 1103 8 -0.07356672503031803 1103 9 0.026897107746762572 1103 10-11 -0.15061073376536882 1103 12-13 -0.3055762188036013 1103 14-15 -0.4204478774998819 1103 16-17 -0.6008967345994805 1103 18-19 -5.250352118437718E-4 1103 20-21 -0.0643176283512048 1103 22-23 0.1699521315450525 1103 24-25 -0.13521736918318084 1103 26-27 -0.13037730002331216 1103 28-29 -0.5692829880991042 1103 30-31 -1.0494126847235492 1103 32-33 -0.9609734459264772 1103 34-35 -1.0501911696075936 1103 36-37 -0.8494038501549568 1103 38-39 -1.5280828162151323 1103 40-41 -0.8678995462806753 1103 42-43 -0.44454992073185906 1103 44-45 -0.5561823534888077 1103 46-47 -0.6139423303769362 1103 48-49 -0.8281143116433185 1103 50-51 -1.1038004399309287 1103 52-53 -0.9842534071300975 1103 54-55 -0.2656633082324049 1103 56-57 -0.7326575281056904 1103 58-59 -1.373638718734938 1103 60-61 -1.2983069665336942 1103 62-63 -0.6061689495298808 1103 64-65 -1.2173211428264459 1103 66-67 -1.3260518060608284 1103 68-69 -1.271985172112938 1103 70-71 -0.7605165086848906 1103 72-73 -0.6606019356235215 1103 74-75 -1.0162227881666546 1103 76-77 -0.8023345661807362 1103 78-79 -1.5471517060318192 1103 80-81 -1.5220863290577462 1103 82-83 -1.6165454066260274 1103 84-85 -1.944060600436778 1103 86-87 -1.7897695099480657 1103 88-89 -1.8577654305070403 1103 90-91 -1.8395681566296567 1103 92-93 -1.6200757215332189 1103 94-95 -1.8273934824070643 1103 96-97 -2.0791648400843066 1103 98-99 -2.3486637262222665 1103 100-101 -1.514062498243927 1104 1 0.49689471295568666 1104 2 0.12261807873970554 1104 3 0.2001772648628446 1104 4 0.3643097658043928 1104 5 0.38180506043732976 1104 6 0.15913288110152735 1104 7 0.09215706179899996 1104 8 0.06890639324925019 1104 9 -0.07129191319268102 1104 10-11 0.20726985480282423 1104 12-13 0.4157027851579187 1104 14-15 0.16932292046276132 1104 16-17 0.36486444253690564 1104 18-19 0.41875906212827374 1104 20-21 0.3302777310149523 1104 22-23 0.5844258157555728 1104 24-25 0.0071850077268393875 1104 26-27 0.5023335092579586 1104 28-29 0.3451199547418753 1104 30-31 0.7609297035050915 1104 32-33 0.5143378160995553 1104 34-35 0.43912688358992114 1104 36-37 0.810896093251948 1104 38-39 0.4762324073275934 1104 40-41 0.35521873328921316 1104 42-43 0.02800242788161711 1104 44-45 0.5861492820516645 1104 46-47 0.15006446079454605 1104 48-49 0.061812117332344485 1104 50-51 0.33083453460217527 1104 52-53 0.6278009222417182 1104 54-55 -0.4545427649386795 1104 56-57 -0.21016177258785973 1104 58-59 0.4676883893578676 1104 60-61 0.2687558518024744 1104 62-63 0.17410835663876156 1104 64-65 -0.13257298210204738 1104 66-67 0.1485520422696709 1104 68-69 0.3056181102866091 1104 70-71 0.05697075786859074 1104 72-73 0.4967268702621581 1104 74-75 0.26462044895841785 1104 76-77 0.5890066901859861 1104 78-79 0.6615353341492778 1104 80-81 0.7724807337605917 1104 82-83 0.4479707224062288 1104 84-85 0.2721957662864831 1104 86-87 0.3945824991068321 1104 88-89 0.6298548298212019 1104 90-91 0.40830960228375446 1104 92-93 0.8215626485856262 1104 94-95 0.4833026126693376 1104 96-97 0.5878555334301225 1104 98-99 1.0663051475751324 1104 100-101 1.6805470093961397 1105 1 0.012245590148665997 1105 2 0.2541970261081232 1105 3 0.5148702473189815 1105 4 0.39501152019035857 1105 5 0.19649804289346662 1105 6 0.3345714775927604 1105 7 0.09983250039549318 1105 8 0.4022397265825859 1105 9 0.19625194645644228 1105 10-11 0.2966338898905505 1105 12-13 0.47600980270177473 1105 14-15 0.6249150257259259 1105 16-17 0.7831758460456797 1105 18-19 0.9988029217774042 1105 20-21 0.3143786082079387 1105 22-23 -0.06963120178827609 1105 24-25 0.4830622007092984 1105 26-27 -0.07661385916309627 1105 28-29 -0.6663932031528645 1105 30-31 0.11893408946999529 1105 32-33 -0.050355166356588654 1105 34-35 -0.08883364272587357 1105 36-37 -1.3184898716603257 1105 38-39 -1.1564430312688998 1105 40-41 -0.9989479333774582 1105 42-43 -0.7066466949253964 1105 44-45 -1.0766796653167603 1105 46-47 -0.8027864163984333 1105 48-49 0.07880773136744068 1105 50-51 0.4371941837249764 1105 52-53 0.0779105713645265 1105 54-55 0.16881249821921074 1105 56-57 0.5546408589910854 1105 58-59 0.3706489156736623 1105 60-61 0.2934269044340496 1105 62-63 0.7590864268141999 1105 64-65 0.5269664915821579 1105 66-67 0.5197143229714243 1105 68-69 -0.1691625914677779 1105 70-71 -0.2527880140612311 1105 72-73 -0.11456698938696164 1105 74-75 -0.8845023580591302 1105 76-77 -0.3490415554280517 1105 78-79 -0.37410063076300304 1105 80-81 -0.37390084518677824 1105 82-83 -0.23952927759377118 1105 84-85 0.16145015225139758 1105 86-87 -0.0017990798405378428 1105 88-89 -0.1333030649156406 1105 90-91 0.10129205842410371 1105 92-93 0.2223301924452734 1105 94-95 0.42025436705529984 1105 96-97 0.5209695685178417 1105 98-99 0.8206911124874132 1105 100-101 -0.16594421867403852 1106 1 -0.25310528704431334 1106 2 0.10946018400286306 1106 3 -0.27350694566347045 1106 4 -0.05674286577455945 1106 5 0.26338400780574744 1106 6 -0.380340803108993 1106 7 -0.5394218855694248 1106 8 -0.1810936067507498 1106 9 -0.2818182289821536 1106 10-11 -0.0229933030919085 1106 12-13 0.20517646936844613 1106 14-15 -0.04778234269512893 1106 16-17 -0.1088197679894094 1106 18-19 0.0700748516019587 1106 20-21 0.014488257330739884 1106 22-23 -0.014258394770735094 1106 24-25 0.3821850077268394 1106 26-27 -0.08977175389993874 1106 28-29 -0.2206695189423371 1106 30-31 -0.0614387175475386 1106 32-33 -0.6238200786372872 1106 34-35 -0.883241537462716 1106 36-37 0.35036977746248255 1106 38-39 -0.09613601372503666 1106 40-41 0.4473239964471034 1106 42-43 -0.419365993171013 1106 44-45 -1.8875349284746505 1106 46-47 -2.0078302760475566 1106 48-49 -1.2539773563518608 1106 50-51 -2.1296917811872973 1106 52-53 -0.4774622356530074 1106 54-55 -1.5334901333597344 1106 56-57 -2.183845983114182 1106 58-59 -1.8941537159052864 1106 60-61 -1.4680862534606867 1106 62-63 -2.1022074328349163 1106 64-65 -2.0799414031546846 1106 66-67 -2.489605852467168 1106 68-69 -2.700960837081812 1106 70-71 -1.535134505289303 1106 72-73 -1.2137994455273144 1106 74-75 -1.6301163931468459 1106 76-77 -1.608361730866644 1106 78-79 -1.7595172974296673 1106 80-81 -2.6222561083446756 1106 82-83 -1.5651871723306137 1106 84-85 -1.1817516021345682 1106 86-87 -0.9343648693142228 1106 88-89 -1.4293556964945857 1106 90-91 -3.328532502979403 1106 92-93 -2.5797531408880587 1106 94-95 -2.6219605452254022 1106 96-97 -2.4713549928856686 1106 98-99 -4.24948432610908 1106 100-101 -4.49708456955123 1107 1 -0.0999338970308159 1107 2 0.427273949185043 1107 3 0.30813947808821496 1107 4 0.19629357147240967 1107 5 0.15002368391910892 1107 6 0.3409817340030159 1107 7 -0.4530521149891271 1107 8 -0.4887859144430564 1107 9 0.11291861312310658 1107 10-11 -0.1721161101094566 1107 12-13 0.30774057193254833 1107 14-15 0.12090861546951714 1107 16-17 -0.6951895385697 1107 18-19 -0.5220304115559316 1107 20-21 0.2847311723104937 1107 22-23 0.1314905930835124 1107 24-25 0.790754508401605 1107 26-27 -0.19199847454770946 1107 28-29 0.5667798737702157 1107 30-31 0.4017866535725645 1107 32-33 0.26855509005367395 1107 34-35 1.1491471265049 1107 36-37 0.9347152565447985 1107 38-39 0.6464415841157205 1107 40-41 0.5923982204687022 1107 42-43 0.5145071512284431 1107 44-45 0.6596985398114441 1107 46-47 0.4071494810374574 1107 48-49 -0.5501986788889752 1107 50-51 -0.9073570983263011 1107 52-53 -0.9950060953021378 1107 54-55 -1.1701298094730959 1107 56-57 -0.705775807675586 1107 58-59 -1.1589985202237685 1107 60-61 -1.132053864796724 1107 62-63 -0.7128687013909243 1107 64-65 -1.4898604314947654 1107 66-67 -0.6076895231824153 1107 68-69 -1.1226882324934166 1107 70-71 -1.013204680727899 1107 72-73 -1.3285092970792718 1107 74-75 -0.9037331272898967 1107 76-77 -0.9636248887613839 1107 78-79 -1.5095172974296673 1107 80-81 -0.8570739221098549 1107 82-83 -0.6930549186194135 1107 84-85 -0.3882293349280914 1107 86-87 -0.3311260029174612 1107 88-89 -0.4570210136335895 1107 90-91 0.3961638532958993 1107 92-93 0.054862243727328064 1107 94-95 0.2383633414142743 1107 96-97 -0.11845350840523494 1107 98-99 -0.12161657982028018 1107 100-101 -0.18196985969967727 1108 1 -0.044126958870940314 1108 2 -0.054626503303644824 1108 3 -0.11870818405356687 1108 4 -0.05596887196650613 1108 5 0.004096082109157351 1108 6 -0.04287950279940134 1108 7 -0.20041259764372654 1108 8 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1.537423842517633 2316 52-53 1.6217918049603313 2316 54-55 1.4761451753845591 2316 56-57 1.8900247172588038 2316 58-59 1.6351666322090992 2316 60-61 1.4245784792371978 2316 62-63 1.1607432509611826 2316 64-65 0.5080196411884543 2316 66-67 1.0232412258585697 2316 68-69 0.4855290095820912 2316 70-71 0.8909947943377148 2316 72-73 0.9699966589070002 2316 74-75 0.7972561721245981 2316 76-77 0.8867688120260162 2316 78-79 0.6715850647750585 2316 80-81 0.6332349815848737 2316 82-83 0.7826163916975659 2316 84-85 0.7577428031700322 2316 86-87 0.46596666819095844 2316 88-89 0.6609882736670336 2316 90-91 0.4503734232535024 2316 92-93 0.6190657567497375 2316 94-95 0.5605922935644863 2316 96-97 1.0516454215362145 2316 98-99 0.6389325823036849 2316 100-101 -0.18792584597062145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 0.0 5 1.0 6 4.0 7 9.0 8 21.0 9 50.0 10 100.0 11 179.0 12 286.0 13 438.0 14 579.0 15 885.0 16 1344.0 17 1879.0 18 1656.0 19 959.0 20 788.0 21 730.0 22 792.0 23 762.0 24 806.0 25 876.0 26 959.0 27 1011.0 28 1155.0 29 1395.0 30 1646.0 31 2100.0 32 2789.0 33 3596.0 34 5421.0 35 8467.0 36 14146.0 37 23626.0 38 22703.0 39 2653.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.806141833004 20.75088060941035 15.681052340177745 19.761925217407907 2 15.687135060870894 22.187344960500706 39.82368431095676 22.30183566767164 3 19.13425943594245 26.61718123878945 31.79502347059497 22.453535854673127 4 12.641682250124031 18.67248025035301 38.319085600885394 30.366751898637563 5 12.84434308832931 38.94142231467257 33.961508000877856 14.252726596120265 6 29.417433118345226 38.32003969011182 17.533297714002213 14.72922947754074 7 26.588558561996717 35.624737625462735 21.029080639621416 16.757623172919132 8 26.44830744571232 34.76319505400145 19.7448765408541 19.043620959432126 9 26.637217112544363 17.165019272602375 20.94989123382819 35.247872381025076 10-11 24.384135404343013 28.269663778956613 26.981643323283595 20.364557493416786 12-13 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31.66908369270694 19.535453955653935 38-39 20.41512422241728 28.3669808800519 31.71344884173568 19.50444605579514 40-41 20.001526542762278 29.21850551463573 31.256440102278365 19.523527840323627 42-43 20.207461554243945 29.2583774292517 30.84153620795778 19.692624808546576 44-45 20.614528870739992 29.01957791092623 30.238426897683475 20.12746632065031 46-47 20.7514406747319 29.500438881044154 29.91642178376522 19.831698660458727 48-49 20.756688165477236 29.67122085257413 29.177002633286264 20.395088348662366 50-51 20.552990115635613 29.700320573980076 29.174140365606995 20.572548944777317 52-53 20.629794298362782 29.961263977407164 28.792027630424 20.616914093806052 54-55 20.347860931954358 29.995134144945233 28.663702629469906 20.9933022936305 56-57 20.63361065526848 29.686963324810133 29.085410067549518 20.594015952371866 58-59 20.786741976109603 29.082070755257032 28.844502537877343 21.28668473075602 60-61 20.19234438804717 28.938003282066937 29.72369576002748 21.145956569858413 62-63 19.834560928137996 28.61313590046941 30.23031713925886 21.321986032133726 64-65 19.582681372361943 28.2186200053429 30.49555394420486 21.703144678090293 66-67 19.582681372361943 28.01015150936916 30.26084799450445 22.146319123764453 68-69 19.444338434530398 27.83793840399954 29.909743159180245 22.807980002289817 70-71 19.359901537991835 28.123211082700454 29.926439720642673 22.59044765866504 72-73 18.933423653780103 27.5359691638362 30.281837957485784 23.24876922489791 74-75 18.357630805632944 27.441991375033393 29.95744762050147 24.242930198832195 76-77 18.433480899133688 26.3967866274854 30.59334808991337 24.57638438346754 78-79 18.629546235163915 26.996908750906385 29.813380147311374 24.560164866618326 80-81 18.06138610082815 26.526542762279128 29.599187115979085 25.812884020913636 82-83 17.902138601203113 25.81873172314107 29.818769886892184 26.460359788763636 84-85 17.318875494344553 25.939195023399375 30.190200409310137 26.551729072945935 86-87 17.4898866541999 26.046635881387626 29.146948822653897 27.316528641758573 88-89 16.50478952791665 25.2614204480403 29.384517040033586 28.849272984009467 90-91 16.79398054268633 25.35605738905562 29.78858421562406 28.06137785263399 92-93 16.102394321179652 25.82995024353476 29.309086398786384 28.758569036499203 94-95 15.202457733847268 26.840438117772774 27.904724649849257 30.0523794985307 96-97 15.701923443880473 28.43567530435446 26.94920428958516 28.913196962179903 98-99 15.755025712949976 28.949728559025296 25.92428275658089 29.370962971443838 100-101 15.25567680276361 27.047299134940666 24.67828667675028 33.01873738554544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 74.0 1 46.5 2 28.0 3 39.0 4 42.5 5 39.5 6 49.0 7 85.5 8 129.0 9 159.0 10 182.0 11 185.0 12 175.5 13 192.5 14 230.0 15 279.0 16 291.5 17 304.5 18 329.0 19 347.0 20 381.0 21 418.5 22 507.5 23 587.5 24 630.0 25 749.0 26 869.5 27 1020.0 28 1204.0 29 1367.5 30 1575.0 31 1789.5 32 1983.5 33 2180.0 34 2348.5 35 2499.5 36 2802.5 37 3056.0 38 3145.0 39 3233.0 40 3341.0 41 3526.0 42 3668.0 43 3850.5 44 4048.5 45 4069.0 46 4031.5 47 4242.0 48 4439.0 49 4185.0 50 3872.5 51 3557.5 52 3298.5 53 3187.0 54 2897.0 55 2543.5 56 2210.5 57 1867.5 58 1582.0 59 1267.0 60 1018.0 61 772.5 62 534.5 63 399.5 64 262.0 65 178.5 66 127.5 67 93.0 68 72.5 69 43.5 70 26.5 71 17.5 72 16.0 73 13.5 74 9.5 75 9.0 76 6.5 77 3.5 78 2.5 79 1.5 80 1.5 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05247490745334504 2 0.0 3 0.0 4 0.0 5 0.010494981490669008 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002385223066061138 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.540892264244552E-4 24-25 0.0 26-27 0.0019081784528489105 28-29 0.002385223066061138 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.020512918368125785 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.770446132122276E-4 84-85 0.001431133839636683 86-87 0.0 88-89 0.0 90-91 0.01765065068885242 92-93 0.001431133839636683 94-95 0.0 96-97 0.0 98-99 9.540892264244552E-4 100-101 0.020512918368125785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 104812.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.13609128725719 #Duplication Level Percentage of deduplicated Percentage of total 1 90.0959753834131 70.39747357172843 2 6.1761258181107745 9.65156661450979 3 1.5580736543909348 3.6522535587528147 4 0.7326365146038879 2.2898141434186927 5 0.3931815961707531 1.536083654543373 6 0.24543323239230244 1.150631607067893 7 0.16972745921656737 0.9283288173109949 8 0.11844290319429521 0.7403732397053773 9 0.09035850346781284 0.6354234247986872 >10 0.39196053531308 5.287562492844331 >50 0.01709485200742405 0.8233790024043048 >100 0.008547426003712026 1.4721596763729343 >500 0.002442121715346293 1.4349501965423805 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 823 0.7852154333473267 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 681 0.649734763195054 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 443 0.4226615273060337 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 302 0.28813494638018544 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 235 0.22421096820974695 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 205 0.1955882914170133 No Hit GCTAAGGGTAGTTTATTTTGGACACAGACATATTAGGGCATTGTCACCAC 133 0.12689386711445255 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 118 0.11258252871808572 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 107 0.10208754722741671 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 9.540892264244552E-4 0.0 24-25 0.0 0.0 0.0 0.002862267679273366 0.0 26-27 0.0 0.0 0.0 0.003816356905697821 0.0 28-29 0.0 0.0 0.0 0.011449070717093463 0.0 30-31 0.0 0.0 0.0 0.02814563217952143 0.0 32-33 0.0 0.0 0.0 0.04341105980231272 0.0 34-35 0.0 0.0 0.0 0.06010762126474068 0.0 36-37 0.0 0.0 0.0 0.07680418272716864 0.0 38-39 0.0 0.0 0.0 0.11067435026523681 0.0 40-41 0.0 0.0 0.0 0.20322100522840897 0.0 42-43 0.0 0.0 0.0 0.38497500286226766 0.0 44-45 0.0 0.0 0.0 0.544784948288364 0.0 46-47 0.0 0.0 0.0 0.949318780292333 0.0 48-49 0.0 0.0 0.0 1.3481280769377553 0.0 50-51 0.0 0.0 0.0 1.6124107926573292 0.0 52-53 0.0 0.0 0.0 1.9840285463496548 0.0 54-55 0.0 0.0 0.0 2.3613708354005265 0.0 56-57 0.0 0.0 0.0 3.286837385032248 0.0 58-59 0.0 0.0 0.0 4.416001984505591 0.0 60-61 0.0 0.0 0.0 5.619108499026829 0.0 62-63 0.0 0.0 0.0 6.998244475823379 0.0 64-65 0.0 0.0 0.0 8.441304430790368 0.0 66-67 0.0 0.0 0.0 10.190627027439607 0.0 68-69 0.0 0.0 0.0 11.948536427126665 0.0 70-71 0.0 0.0 0.0 13.719326031370453 0.0 72-73 0.0 0.0 0.0 15.522554669312674 0.0 74-75 0.0 0.0 0.0 17.44933786207686 0.0 76-77 0.0 0.0 0.0 19.989600427431974 0.0 78-79 0.0 0.0 0.0 21.98316986604587 0.0 80-81 0.0 0.0 0.0 24.42420715185284 0.0 82-83 0.0 0.0 0.0 27.362801969240163 0.0 84-85 0.0 0.0 0.0 31.055127275502805 0.0 86-87 0.0 0.0 0.0 34.16116475212762 0.0 88-89 0.0 0.0 0.0 37.75426477884211 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCGC 20 0.0020696293 71.24284 9 AGGGTAG 35 2.7776163E-4 54.280262 5 CTAAGGG 35 2.7776163E-4 54.280262 2 TATATCA 40 5.368316E-4 47.495228 5 AAGGGTA 40 5.368316E-4 47.495228 4 TAAGGGT 45 9.589761E-4 42.21798 3 TACTCAC 25 0.0016098182 38.01432 90-91 GTACCTT 50 0.0016099161 37.996185 6 TAGTTTA 50 0.0016099161 37.996185 9 GCTAAGG 55 0.002546265 34.60803 1 ATGGGGA 55 0.0025702526 34.541985 5 GGGTAGT 55 0.0025702526 34.541985 6 GGTATCA 700 0.0 30.591026 1 CATGGGG 140 1.6765625E-8 30.532646 4 GGTAGTT 65 0.0058230436 29.227833 7 CTGGACT 65 0.0058230436 29.227833 4 GTATATG 85 6.3009834E-4 27.991789 1 CTCCTGG 70 0.008283899 27.192022 1 TACATGG 585 0.0 25.980295 2 GTACATG 600 0.0 25.379223 1 >>END_MODULE