FastQCFastQC Report
Thu 26 May 2016
SRR937308_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937308_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183698
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14970.8149244956395824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11260.6129625798865529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.3854151923265359No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.15895654824766736No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2450.1333710764406798No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2340.12738298729436356No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2340.12738298729436356No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2020.10996309159598908No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT250.005010549457.011486
GGTATCA8000.043.3524781
GGACCAT459.594312E-442.230736
GTAGTCG250.00161494737.9972746-47
GTATCAA13350.037.7229541
CGGGAGT550.002571439834.5524184
GTCTTAT600.003938461731.6730461
TATCAAC15950.031.5651282
ATCAACG16300.030.5959593
ATGGGAT3500.029.863165
TCAACGC16550.029.8549564
CAACGCA16650.029.6756485
AACGCAG16800.029.4106866
TGGCCTA650.005825662529.236665
GGACAGT1205.3409203E-627.7139156
TAATAGA700.00836523427.1483236
TGGACCA700.00836523427.1483235
GCTCGAT350.0083871727.14090346-47
GTGTAGC908.908213E-426.3942051
ATAACAG908.908213E-426.3942055