##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937308_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 183698 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04537338457686 33.0 31.0 34.0 31.0 34.0 2 32.15213012662087 33.0 31.0 34.0 31.0 34.0 3 32.174095526353035 34.0 31.0 34.0 30.0 34.0 4 35.76688913325131 37.0 35.0 37.0 35.0 37.0 5 35.77555008764385 37.0 35.0 37.0 35.0 37.0 6 35.8358011518906 37.0 36.0 37.0 35.0 37.0 7 35.69866846672256 37.0 35.0 37.0 35.0 37.0 8 35.72287667802589 37.0 35.0 37.0 35.0 37.0 9 37.41807205304358 39.0 37.0 39.0 35.0 39.0 10-11 37.33357467147165 39.0 37.0 39.0 35.0 39.0 12-13 37.20550577578417 39.0 37.0 39.0 34.0 39.0 14-15 38.42038018922362 40.0 38.0 41.0 34.0 41.0 16-17 38.38523827151085 40.0 38.0 41.0 34.0 41.0 18-19 38.395643937331926 40.0 38.0 41.0 34.0 41.0 20-21 38.33021589783231 40.0 38.0 41.0 34.0 41.0 22-23 38.16908186262235 40.0 38.0 41.0 34.0 41.0 24-25 38.07159304946161 40.0 38.0 41.0 34.0 41.0 26-27 38.05922492351577 40.0 38.0 41.0 34.0 41.0 28-29 37.95845354875938 40.0 38.0 41.0 34.0 41.0 30-31 37.69841533386319 40.0 37.5 41.0 33.0 41.0 32-33 37.40961523805376 40.0 36.5 41.0 33.0 41.0 34-35 37.43980609478601 40.0 37.0 41.0 33.0 41.0 36-37 37.3345735936156 40.0 37.0 41.0 33.0 41.0 38-39 37.18516532569761 40.0 36.5 41.0 32.5 41.0 40-41 36.92238075537023 40.0 36.0 41.0 31.5 41.0 42-43 36.582992193709245 40.0 36.0 41.0 31.0 41.0 44-45 36.242776187002576 39.5 36.0 41.0 30.0 41.0 46-47 35.77004376748794 39.0 35.0 41.0 28.0 41.0 48-49 35.314750296682604 39.0 35.0 41.0 26.0 41.0 50-51 34.28522085161515 38.0 34.0 40.0 23.5 40.5 52-53 34.354701738723335 38.5 34.5 39.5 23.0 40.5 54-55 34.69123234874631 39.0 35.0 40.0 23.0 41.0 56-57 34.46112097028819 39.0 35.0 40.0 21.5 41.0 58-59 34.18281636163704 38.0 34.0 40.0 19.5 41.0 60-61 33.84599723459156 38.0 34.0 40.0 16.0 41.0 62-63 33.54498688064105 38.0 34.0 40.0 11.5 41.0 64-65 33.11144922644776 37.0 33.0 40.0 7.5 41.0 66-67 32.742237258979415 37.0 33.0 40.0 3.5 41.0 68-69 32.25860923907718 36.0 32.5 39.0 2.0 41.0 70-71 31.777564263083974 36.0 32.0 39.0 2.0 40.5 72-73 31.305871593593835 35.0 31.0 38.5 2.0 40.0 74-75 30.84207231434202 35.0 31.0 37.5 2.0 40.0 76-77 30.351645091400016 35.0 30.5 37.0 2.0 39.0 78-79 29.833234439133797 35.0 30.0 36.5 2.0 39.0 80-81 29.359911376280635 34.0 29.0 36.0 2.0 38.5 82-83 28.885575781990006 34.0 29.0 35.5 2.0 37.0 84-85 28.363882568128123 34.0 29.0 35.0 2.0 37.0 86-87 27.917094905769254 34.0 27.5 35.0 2.0 36.5 88-89 27.44467277814674 34.0 26.5 35.0 2.0 36.0 90-91 26.929754270596305 33.5 25.5 35.0 2.0 35.5 92-93 26.41888316693704 33.0 25.0 35.0 2.0 35.0 94-95 25.875845137127243 33.0 23.5 35.0 2.0 35.0 96-97 25.1693213861882 32.5 19.0 35.0 2.0 35.0 98-99 24.249275985585037 32.0 2.0 34.0 2.0 35.0 100-101 21.92033391762567 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18886705732256104 1101 2 0.24803099495152026 1101 3 -0.07251803231863363 1101 4 0.014479803496250554 1101 5 -0.03289522124821076 1101 6 -0.1128100304930868 1101 7 -0.1055401312487021 1101 8 -0.021174603802236902 1101 9 -0.2592470130379354 1101 10-11 -0.12166275581601127 1101 12-13 0.06813754010811124 1101 14-15 0.09828133948980167 1101 16-17 -0.05116541356144921 1101 18-19 0.17901593868472787 1101 20-21 0.1399719865232001 1101 22-23 0.061759799165400864 1101 24-25 -0.014985114948423472 1101 26-27 -9.179004126949053E-4 1101 28-29 0.04481325823385873 1101 30-31 0.40975177954555164 1101 32-33 0.25077454400256727 1101 34-35 -0.03837881776962604 1101 36-37 0.4591690976699283 1101 38-39 0.6446134773994459 1101 40-41 0.6290309977883197 1101 42-43 0.7792594226008305 1101 44-45 1.1204700167588513 1101 46-47 1.4762420127167175 1101 48-49 1.7288924212075116 1101 50-51 1.9236838400529166 1101 52-53 1.82057622387714 1101 54-55 1.8714766874537574 1101 56-57 1.8652960496167381 1101 58-59 2.1822755746154243 1101 60-61 2.2920610447403647 1101 62-63 2.132757932310305 1101 64-65 2.2672295687519153 1101 66-67 2.4460233886519553 1101 68-69 2.3808566767330284 1101 70-71 2.6440119295907216 1101 72-73 2.3618313813755414 1101 74-75 2.123051942964512 1101 76-77 2.2417593604561397 1101 78-79 2.4690681118474913 1101 80-81 2.3690996689487847 1101 82-83 2.403493907355717 1101 84-85 1.797397082397442 1101 86-87 1.733912786593777 1101 88-89 1.6973707421559467 1101 90-91 1.925684410595487 1101 92-93 2.010560207025911 1101 94-95 2.077712717197457 1101 96-97 2.413756271313524 1101 98-99 1.9088545714626513 1101 100-101 1.511947866034408 1102 1 0.052941674803172134 1102 2 -0.07286609161313606 1102 3 -0.0990895534405638 1102 4 -0.004527075183652585 1102 5 -0.14903248997403296 1102 6 -0.10143448505759523 1102 7 0.02018155850569059 1102 8 0.05431938449829232 1102 9 -0.3694678548664072 1102 10-11 -0.06639037152455529 1102 12-13 0.024293101168304077 1102 14-15 0.022358624602503596 1102 16-17 -0.1453709355598889 1102 18-19 -0.008394743276369354 1102 20-21 -0.10630285580068488 1102 22-23 -0.4951417461557668 1102 24-25 -0.18225993821778985 1102 26-27 -0.36009074391796503 1102 28-29 -0.11352976114457647 1102 30-31 -0.0684355644456005 1102 32-33 0.16271410308109324 1102 34-35 0.46790917216959826 1102 36-37 0.41252364502992833 1102 38-39 0.32434995345744255 1102 40-41 0.2735880755879023 1102 42-43 0.25507447947855155 1102 44-45 1.0716380585263465 1102 46-47 1.186399021920053 1102 48-49 0.9153503774202321 1102 50-51 0.8579267089006919 1102 52-53 1.1864800747946163 1102 54-55 1.2204124927928248 1102 56-57 1.4241840281232427 1102 58-59 1.3782988973417432 1102 60-61 1.8321469806028645 1102 62-63 1.609835350804616 1102 64-65 1.9975176730182227 1102 66-67 1.544668612637782 1102 68-69 1.36559877387295 1102 70-71 1.1051788280873218 1102 72-73 1.1035093219635677 1102 74-75 1.4713684765671466 1102 76-77 1.1258174005087263 1102 78-79 1.2854206965924497 1102 80-81 1.301413268156118 1102 82-83 1.1781885587533694 1102 84-85 0.8498208167012855 1102 86-87 0.9415212544202305 1102 88-89 0.6730762355726121 1102 90-91 1.0659494588986789 1102 92-93 1.4693691092953038 1102 94-95 1.2368226808033356 1102 96-97 1.4487689425659802 1102 98-99 1.1221555756355848 1102 100-101 1.0206176703445387 1103 1 -0.20024379003873705 1103 2 -0.03322475135830416 1103 3 0.17361626834501465 1103 4 0.045689579327479635 1103 5 -0.2068642287873672 1103 6 -0.34134181698946264 1103 7 -0.16438234384962414 1103 8 -0.28447678865371273 1103 9 -0.08191798142696172 1103 10-11 0.13133368356147201 1103 12-13 -0.12015754021485492 1103 14-15 -0.44194700841478607 1103 16-17 -0.18559145124052634 1103 18-19 -0.5472423986884394 1103 20-21 -0.26562972525025685 1103 22-23 -0.11459131499184139 1103 24-25 -0.026837140156963812 1103 26-27 -0.29021430134213944 1103 28-29 -0.40882342453640774 1103 30-31 -0.3083321709066311 1103 32-33 -0.5085073135390914 1103 34-35 0.1588011023253273 1103 36-37 -0.23673200729320598 1103 38-39 -0.23257012033379 1103 40-41 -0.09777062705914119 1103 42-43 0.2717954885308558 1103 44-45 0.522268716212217 1103 46-47 0.49513170028463094 1103 48-49 -0.18255172048186097 1103 50-51 -0.8274737801807817 1103 52-53 -0.7213237777945096 1103 54-55 -0.576125325217383 1103 56-57 -0.535123535478796 1103 58-59 -0.6871264748320982 1103 60-61 -1.05329426526783 1103 62-63 -0.8567440820324919 1103 64-65 -0.9184911647697547 1103 66-67 -0.767120824918532 1103 68-69 -0.41685650091361737 1103 70-71 -0.6129128961393739 1103 72-73 -1.0530396505363235 1103 74-75 -0.9961118958211976 1103 76-77 -0.9764499640285784 1103 78-79 -1.0867316908973628 1103 80-81 -0.8507060848834769 1103 82-83 -0.8616136635641922 1103 84-85 -0.7578092832601016 1103 86-87 -0.5653215959809046 1103 88-89 -0.46895044939886077 1103 90-91 -0.8597337535943481 1103 92-93 -0.6075003402735284 1103 94-95 -0.7339504111354032 1103 96-97 -0.9635610587210799 1103 98-99 -0.008941854541600236 1103 100-101 0.09479502673048401 1104 1 0.08931006831441834 1104 2 -0.2790408692164874 1104 3 0.018530921322764016 1104 4 -0.10359467425125501 1104 5 0.07079530128233102 1104 6 0.04808266445955667 1104 7 0.052720448187436375 1104 8 0.05265438283328905 1104 9 0.20450579053580498 1104 10-11 -0.206214832777583 1104 12-13 -0.06352384379150067 1104 14-15 -0.10782071986255204 1104 16-17 -0.47401847849314294 1104 18-19 -0.2775541552929184 1104 20-21 -0.5259903112024276 1104 22-23 -0.47945505189764503 1104 24-25 -0.6416695761988578 1104 26-27 -0.23575079814944644 1104 28-29 0.09445273255219888 1104 30-31 -0.3209568526811779 1104 32-33 -0.6684547280877382 1104 34-35 -0.6822739780135549 1104 36-37 -0.6573112819478553 1104 38-39 -0.3943955867166693 1104 40-41 0.1463343769056351 1104 42-43 -0.3459792287180079 1104 44-45 0.11034934723763712 1104 46-47 -0.12608611199365072 1104 48-49 0.1915979393820848 1104 50-51 -0.15869952201125415 1104 52-53 -0.07606690203807887 1104 54-55 -0.6188197035822256 1104 56-57 -0.3314175131925836 1104 58-59 -0.47974781784783005 1104 60-61 -0.027481486168454694 1104 62-63 -0.11132444178375067 1104 64-65 0.0256680940257894 1104 66-67 -0.25236768439851787 1104 68-69 -0.06098654556951644 1104 70-71 -0.15135035701329258 1104 72-73 -0.3693035794207624 1104 74-75 -0.2846849937720357 1104 76-77 -0.7029847497219954 1104 78-79 -0.625002213830463 1104 80-81 -0.4445669421098053 1104 82-83 -0.5102301653796388 1104 84-85 -0.14003456601124142 1104 86-87 0.19714658147412933 1104 88-89 -0.21080179116255948 1104 90-91 0.1511380798015871 1104 92-93 -0.03271214278595025 1104 94-95 -0.22268210013001521 1104 96-97 0.1083474592873479 1104 98-99 0.7413815877187453 1104 100-101 0.9275440772709409 1105 1 -0.22590447904298472 1105 2 -0.3517122454592929 1105 3 0.1080782787272696 1105 4 -0.15487672553330611 1105 5 -0.03189962284116632 1105 6 -0.13552539414850173 1105 7 0.17752421584834366 1105 8 -0.19917711899821455 1105 9 0.10867899828044614 1105 10-11 -0.35397778411196157 1105 12-13 -0.8135565491813423 1105 14-15 -0.9059630537191765 1105 16-17 -0.4347065998955557 1105 18-19 -0.2028877502693689 1105 20-21 -0.5682783802762117 1105 22-23 -0.4859634244774469 1105 24-25 -0.4886410570989099 1105 26-27 -0.4588669676941919 1105 28-29 -0.2826731177879367 1105 30-31 -0.5601313686414144 1105 32-33 -0.1567789039119205 1105 34-35 -0.15709082783040174 1105 36-37 -0.47602530601902515 1105 38-39 -0.17127941717192385 1105 40-41 -0.7635622740624441 1105 42-43 -0.6749954505913749 1105 44-45 -0.6691836197953336 1105 46-47 -0.18685272633170058 1105 48-49 0.09148674105659893 1105 50-51 0.3911434921174717 1105 52-53 0.8648017531162964 1105 54-55 1.0613215837019183 1105 56-57 0.13178892322814306 1105 58-59 0.33065903720187606 1105 60-61 0.4859931344490178 1105 62-63 0.9197129731822358 1105 64-65 0.618584106584656 1105 66-67 0.7824308504000221 1105 68-69 0.8039728997470696 1105 70-71 1.1296543525628415 1105 72-73 0.9584044268586744 1105 74-75 1.1960188262175002 1105 76-77 1.3644537641450896 1105 78-79 0.6494941231658764 1105 80-81 0.5885636178064679 1105 82-83 0.22495429301910264 1105 84-85 0.5976355020159723 1105 86-87 1.0184443313433036 1105 88-89 1.3732510607474353 1105 90-91 1.5812466616958858 1105 92-93 1.2438446337708307 1105 94-95 2.176945058425712 1105 96-97 1.5344332782303134 1105 98-99 1.8017884427684514 1105 100-101 2.1722785095053787 1106 1 0.22378246192302953 1106 2 -0.12653335458272608 1106 3 0.30195126620490953 1106 4 -0.22374971301094604 1106 5 0.051731861953108194 1106 6 -0.6241211007674679 1106 7 -0.8305258735970895 1106 8 -0.12896244994633577 1106 9 -0.6102387119163382 1106 10-11 -1.1918579272246674 1106 12-13 -0.6157703237788397 1106 14-15 -0.3111508891395687 1106 16-17 0.21578535001500398 1106 18-19 0.08255500465013199 1106 20-21 0.19256687911556014 1106 22-23 0.11186752364420016 1106 24-25 0.4533312148152149 1106 26-27 -0.5333302950644949 1106 28-29 -0.0922661410437513 1106 30-31 0.11797686033891353 1106 32-33 0.3207479297195661 1106 34-35 -0.00480996915419496 1106 36-37 0.13678775301496415 1106 38-39 -0.2654207409644158 1106 40-41 -0.7053109323808755 1106 42-43 -0.5851027851173143 1106 44-45 -0.5624081278454227 1106 46-47 -0.6767901145248985 1106 48-49 0.27093665161115865 1106 50-51 0.005633653119261339 1106 52-53 -0.15979574241143268 1106 54-55 -0.6475335146880568 1106 56-57 -0.7889400570938605 1106 58-59 -1.3642202114564341 1106 60-61 -0.8100712662665472 1106 62-63 -1.1398800500735788 1106 64-65 -0.6351055177445026 1106 66-67 -1.4935083267019529 1106 68-69 -1.1587954365677753 1106 70-71 -0.8891291894765097 1106 72-73 -0.7316671294919566 1106 74-75 -0.07522446537462812 1106 76-77 -0.11027789954006195 1106 78-79 -0.27727242424020915 1106 80-81 -0.5479524823724233 1106 82-83 -0.4802111810417209 1106 84-85 -0.0346319398444912 1106 86-87 0.4491241166742519 1106 88-89 -0.1001389213634738 1106 90-91 0.2736661608014259 1106 92-93 0.2577086767046417 1106 94-95 0.10728942336309899 1106 96-97 -0.21869731210957966 1106 98-99 0.15923477550548526 1106 100-101 -0.16437175883093857 1107 1 -0.254750632889138 1107 2 -0.4190199377669863 1107 3 -0.3566653110163216 1107 4 -0.2446203152768902 1107 5 -0.5511303920719399 1107 6 0.04075665713354937 1107 7 0.08778062610475246 1107 8 -0.09020276002385685 1107 9 -0.17016715556570716 1107 10-11 -0.2866700918042753 1107 12-13 -0.5940052671300649 1107 14-15 -0.8226297203858408 1107 16-17 -0.6622707024596508 1107 18-19 -0.4064133912950183 1107 20-21 -1.060265559763387 1107 22-23 -0.3144890655030892 1107 24-25 -0.24985900581686593 1107 26-27 -0.660790044617265 1107 28-29 -0.4124808100956301 1107 30-31 -0.31493906094910074 1107 32-33 -0.0750481346811469 1107 34-35 -0.32055236629194184 1107 36-37 -0.6667304342241493 1107 38-39 0.017823146930638245 1107 40-41 0.39829670029652675 1107 42-43 -0.1621749377708639 1107 44-45 -0.03617079928250888 1107 46-47 0.8227626582836862 1107 48-49 0.6059098179796791 1107 50-51 -0.467830866856886 1107 52-53 -0.10955722124268163 1107 54-55 -0.18867841629808169 1107 56-57 0.12057097451019416 1107 58-59 -0.48825121920837233 1107 60-61 0.030864929320813417 1107 62-63 -0.26778702681776423 1107 64-65 -0.344556919056366 1107 66-67 -0.8009024829333171 1107 68-69 -1.1944245361503647 1107 70-71 -0.35752513461664037 1107 72-73 -0.6890314705772234 1107 74-75 -0.3135965583978866 1107 76-77 -0.07464879995747253 1107 78-79 -0.3008263896546417 1107 80-81 -0.329705612962762 1107 82-83 0.35476198532679604 1107 84-85 0.5351355020159723 1107 86-87 -0.1930941301951563 1107 88-89 -0.7773899648935867 1107 90-91 -0.8081764152271944 1107 92-93 -1.2112835713573773 1107 94-95 -0.5586318646512112 1107 96-97 -0.1899256961286646 1107 98-99 0.5918525453325572 1107 100-101 -0.1594522597253949 1108 1 0.014480136341632033 1108 2 0.24457702864688002 1108 3 -0.09519907771910141 1108 4 0.14523161682856056 1108 5 0.3345684161620852 1108 6 -0.27921445156995617 1108 7 0.5117604021972042 1108 8 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2316 68-69 -0.8917688584946859 2316 70-71 -0.7329830100745163 2316 72-73 -0.5759362324819897 2316 74-75 -0.635025129826456 2316 76-77 -0.5023044776131513 2316 78-79 -0.22619269002093745 2316 80-81 -0.9883594591166087 2316 82-83 -0.5586995531347441 2316 84-85 -0.5630787836983124 2316 86-87 -0.2178194049204336 2316 88-89 -0.157655532659156 2316 90-91 0.30377413422335664 2316 92-93 0.39288309530929055 2316 94-95 0.3589505529312085 2316 96-97 0.32220800350503964 2316 98-99 0.771568662548674 2316 100-101 1.037663124889992 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 794.0 3 148.0 4 115.0 5 111.0 6 136.0 7 168.0 8 170.0 9 238.0 10 310.0 11 420.0 12 617.0 13 898.0 14 1050.0 15 1502.0 16 2104.0 17 2938.0 18 2421.0 19 1529.0 20 1436.0 21 1476.0 22 1452.0 23 1524.0 24 1640.0 25 1879.0 26 2019.0 27 2246.0 28 2575.0 29 3077.0 30 3718.0 31 4774.0 32 6044.0 33 7942.0 34 11141.0 35 16609.0 36 24970.0 37 36575.0 38 32278.0 39 4653.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.523508236064146 19.722373731864295 15.224719101123597 21.529398930947966 2 14.797342989899873 20.626186165223274 41.543596343884296 23.032874500992563 3 18.381358520198933 26.094472559113512 31.145842422127213 24.378326498560334 4 11.930343157573246 17.09243220838151 38.82392722354327 32.15329741050198 5 12.498500201791032 37.70601773579555 35.089278897021124 14.706203165392292 6 29.253244450748173 37.305592074757385 18.50823454965005 14.93292892484439 7 26.533818467463465 34.21559375289799 21.80048767981147 17.450100099827075 8 25.54711876240972 33.922999716349196 21.011978791647575 19.517902729593505 9 26.039001772807858 16.60930042274649 21.714441565525703 35.63725623891995 10-11 23.703317804423012 27.228962326936294 28.163424506584327 20.904295362056363 12-13 25.265271163046975 25.23144738493096 28.7914000316416 20.71188142038046 14-15 22.013789642494793 25.978311859747116 28.284858667073955 23.723039830684137 16-17 20.131903379955922 29.762541185712106 28.511422898164916 21.594132536167056 18-19 20.01336387934326 27.332678775977744 32.25195003545519 20.402007309223805 20-21 21.009627185207407 26.29339733275152 32.34516049854092 20.351814983500148 22-23 20.52335887855565 26.480759265824855 32.47061390350997 20.525267952109527 24-25 20.237913430491318 26.71235491753207 32.439294441050706 20.610437210925912 26-27 20.03823664172103 26.85046800340366 32.455872406344774 20.655422948530536 28-29 19.811495707381994 27.18012632405721 32.52953561182079 20.47884235674001 30-31 19.79971200418903 26.8793363005629 32.530435920932064 20.79051577431601 32-33 19.727820656175854 27.240297815474406 32.291979163825786 20.73990236452396 34-35 20.181807272290893 27.069537326947625 32.03419045852743 20.714464942234052 36-37 20.2603862705421 26.881309894569196 32.18830279857971 20.670001036308992 38-39 19.916929840350388 27.288520173012838 32.11400738522627 20.6805426014105 40-41 20.152998609103552 27.42929609730821 32.027436114217146 20.390269179371096 42-43 20.130087952546532 27.464648530715213 31.372468807527103 21.032794709211156 44-45 20.065622596429215 27.61849433507345 31.147549926085937 21.1683331424114 46-47 20.333565165214306 27.689999863628305 30.804183883593122 21.172251087564263 48-49 20.275754848212095 27.817145350898727 30.39358481302676 21.513514987862422 50-51 20.26760559538972 27.86540082201719 30.456636820588066 21.410356762005023 52-53 20.45739690159284 28.202323805367662 29.933722452542007 21.40655684049749 54-55 20.099279947632557 27.949487235435306 30.126009164302857 21.825223652629283 56-57 20.307906291760982 27.921291624621595 29.96400032726975 21.806801756347667 58-59 20.534776858151872 27.597049318916266 30.103492002890686 21.76468182004118 60-61 20.10395305205288 27.257951917622936 30.8728565818753 21.765238448448883 62-63 20.004363418192728 26.873747221729822 31.06726481857725 22.054624541500196 64-65 19.84756139130909 26.833285611049757 31.1746499229626 22.144503074678557 66-67 19.76836777546891 26.78005574008323 31.07973231670403 22.371844167743834 68-69 19.66876472641592 26.48573173924426 30.950290164935858 22.89521336940396 70-71 19.640602287076355 26.514376726693555 31.023348033239788 22.821672952990298 72-73 19.503654412566814 26.286953201701756 31.21113777680812 22.998254608923315 74-75 19.44644434990346 26.406388061655267 30.88981248159178 23.257355106849495 76-77 19.33783323106293 26.19622281311788 31.053385150337494 23.41255880548169 78-79 19.36571224422451 26.14330245255387 30.935504896653477 23.555480406568144 80-81 19.087473002159825 26.015282413770535 30.79526365163514 24.101980932434497 82-83 19.2884901209485 25.482907672798177 31.00017726386408 24.228424942389243 84-85 19.195976884539967 25.27510288234187 31.09051191635236 24.4384083167658 86-87 19.420044016221098 25.058974645674876 30.882806129545138 24.63817520855889 88-89 19.09919933678033 24.718022558195344 30.835351353709882 25.34742675131444 90-91 19.12970674782713 25.07465617253144 31.24977841775276 24.545858661888673 92-93 18.76300467691133 25.498575110105126 30.99687751401028 24.741542698973262 94-95 18.769736734460135 25.881505963970746 30.156094049118902 25.19266325245022 96-97 18.849646821392533 26.111489895546402 29.81427441568713 25.22458886737393 98-99 19.151239786623613 26.188787921761993 29.160348645670837 25.499623645943558 100-101 19.79046227011933 25.321233011191342 29.419817186238795 25.468487532450535 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 259.0 1 153.5 2 47.0 3 56.5 4 57.0 5 52.0 6 83.0 7 171.5 8 304.0 9 374.5 10 358.5 11 340.0 12 349.0 13 352.0 14 386.0 15 422.0 16 441.0 17 472.0 18 523.5 19 574.0 20 611.0 21 696.5 22 775.0 23 874.5 24 1049.5 25 1245.0 26 1493.0 27 1830.0 28 2049.0 29 2335.5 30 2715.0 31 3080.0 32 3466.0 33 3791.0 34 4221.5 35 4520.5 36 4860.0 37 5312.0 38 5580.5 39 5680.0 40 5832.5 41 6101.5 42 6360.5 43 6731.5 44 7071.5 45 7249.5 46 7154.5 47 7250.5 48 7705.0 49 7494.0 50 6699.5 51 6065.5 52 5605.0 53 5285.0 54 4952.5 55 4433.5 56 3865.0 57 3305.5 58 2831.5 59 2414.0 60 1917.5 61 1491.0 62 1125.5 63 804.5 64 571.5 65 415.5 66 301.0 67 223.0 68 172.5 69 105.5 70 72.5 71 61.5 72 48.0 73 38.0 74 26.0 75 22.0 76 21.0 77 10.0 78 6.0 79 6.0 80 4.0 81 3.5 82 3.5 83 2.0 84 1.5 85 1.0 86 0.5 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1948850831255648 2 0.18182016135178392 3 0.17528770046489345 4 0.18617513527637752 5 0.18508639179522912 6 0.20958312012106828 7 0.20740563315877147 8 0.20359503097475204 9 0.20305065923417787 10-11 0.20903874838049405 12-13 0.2150268375268103 14-15 0.2025062874936036 16-17 0.20795000489934568 18-19 0.20032880053130683 20-21 0.1976069418284358 22-23 0.1976069418284358 24-25 0.19270759616326796 26-27 0.1992400570501584 28-29 0.19597382660671317 30-31 0.19706257008786163 32-33 0.1976069418284358 34-35 0.2025062874936036 36-37 0.1932519679038422 38-39 0.195429454866139 40-41 0.1976069418284358 42-43 0.1962460124770003 44-45 0.20522814619647464 46-47 0.2044115885856133 48-49 0.20849437663991985 50-51 0.2006009864015939 52-53 0.20577251793704884 54-55 0.20577251793704884 56-57 0.1976069418284358 58-59 0.19080229507125826 60-61 0.18835262223867436 62-63 0.19352415377412927 64-65 0.18808043636838725 66-67 0.18889699397924856 68-69 0.19270759616326796 70-71 0.1978791276987229 72-73 0.1948850831255648 74-75 0.19379633964441637 76-77 0.1962460124770003 78-79 0.1951572689958519 80-81 0.19053010920097116 82-83 0.19352415377412927 84-85 0.1946128972552777 86-87 0.19406852551470347 88-89 0.19053010920097116 90-91 0.1946128972552777 92-93 0.19080229507125826 94-95 0.18889699397924856 96-97 0.1976069418284358 98-99 0.19597382660671317 100-101 0.18617513527637752 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 183698.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.79066959526679 #Duplication Level Percentage of deduplicated Percentage of total 1 84.96722431058544 59.29919478286988 2 9.168893262841083 12.798064005224195 3 2.4377543587546437 5.103975270188005 4 1.1367585572461576 3.17340563513435 5 0.5938759720418648 2.072350087267085 6 0.40532866502181775 1.6972895362816955 7 0.2456123636570703 1.1999015924352174 8 0.18417128096564064 1.028274961504806 9 0.1663374841012729 1.044792395479789 >10 0.6584010540617347 7.849540157359608 >50 0.02277171907320599 1.0869112130583476 >100 0.009900747423133039 1.3446466755318145 >500 9.900747423133038E-4 0.48921369284507954 >1k 0.0019801494846266076 1.812439994820118 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1497 0.8149244956395824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1126 0.6129625798865529 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 708 0.3854151923265359 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 292 0.15895654824766736 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 245 0.1333710764406798 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 234 0.12738298729436356 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 234 0.12738298729436356 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 202 0.10996309159598908 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 5.443717405742033E-4 0.0 0.0 0.0 0.0 24-25 0.0010887434811484066 0.0 0.0 5.443717405742033E-4 0.0 26-27 0.0010887434811484066 0.0 0.0 0.003810602184019423 0.0 28-29 0.0010887434811484066 0.0 0.0 0.00816557610861305 0.0 30-31 0.0010887434811484066 0.0 0.0 0.015514594606364795 0.0 32-33 0.0010887434811484066 0.0 0.0 0.02749077289899727 0.0 34-35 0.0010887434811484066 0.0 0.0 0.03756165009962003 0.0 36-37 0.0010887434811484066 0.0 0.0 0.0498100142625396 0.0 38-39 0.0010887434811484066 0.0 0.0 0.0819279469564176 0.0 40-41 0.0010887434811484066 0.0 0.0 0.14289758190072838 0.0 42-43 0.0010887434811484066 0.0 0.0 0.2585765767727466 0.0 44-45 0.0010887434811484066 0.0 0.0 0.37316682816361635 0.0 46-47 0.0010887434811484066 0.0 0.0 0.6341930777689468 0.0 48-49 0.0010887434811484066 0.0 0.0 0.9300591187710263 0.0 50-51 0.0010887434811484066 0.0 0.0 1.132293220394343 0.0 52-53 0.0010887434811484066 0.0 0.0 1.3957691428322574 0.0 54-55 0.0010887434811484066 0.0 0.0 1.6581563217890234 0.0 56-57 0.0010887434811484066 0.0 0.0 2.273296388637873 0.0 58-59 0.0010887434811484066 0.0 0.0 2.9755359339785956 0.0 60-61 0.0010887434811484066 0.0 0.0 3.7145205718080763 0.0 62-63 0.0010887434811484066 0.0 0.0 4.608923341571492 0.0 64-65 0.0010887434811484066 0.0 0.0 5.5291837690121834 0.0 66-67 0.00163311522172261 0.0 0.0 6.6620213611471 0.0 68-69 0.00163311522172261 0.0 0.0 7.812823220720967 0.0 70-71 0.00163311522172261 0.0 0.0 8.981317161863494 0.0 72-73 0.00163311522172261 0.0 0.0 10.125858746420757 0.0 74-75 0.00163311522172261 0.0 0.0 11.353417021415584 0.0 76-77 0.00163311522172261 0.0 0.0 12.937538786486517 0.0 78-79 0.00163311522172261 0.0 0.0 14.275060153077334 0.0 80-81 0.002177486962296813 0.0 0.0 15.819987152826922 0.0 82-83 0.002177486962296813 0.0 0.0 17.65070931637797 0.0 84-85 0.002177486962296813 0.0 0.0 19.96537795729948 0.0 86-87 0.002177486962296813 0.0 0.0 21.836383629653017 0.0 88-89 0.002177486962296813 0.0 0.0 23.83286698820891 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCGT 25 0.0050105494 57.01148 6 GGTATCA 800 0.0 43.352478 1 GGACCAT 45 9.594312E-4 42.23073 6 GTAGTCG 25 0.001614947 37.99727 46-47 GTATCAA 1335 0.0 37.722954 1 CGGGAGT 55 0.0025714398 34.552418 4 GTCTTAT 60 0.0039384617 31.673046 1 TATCAAC 1595 0.0 31.565128 2 ATCAACG 1630 0.0 30.595959 3 ATGGGAT 350 0.0 29.86316 5 TCAACGC 1655 0.0 29.854956 4 CAACGCA 1665 0.0 29.675648 5 AACGCAG 1680 0.0 29.410686 6 TGGCCTA 65 0.0058256625 29.23666 5 GGACAGT 120 5.3409203E-6 27.713915 6 TAATAGA 70 0.008365234 27.148323 6 TGGACCA 70 0.008365234 27.148323 5 GCTCGAT 35 0.00838717 27.140903 46-47 GTGTAGC 90 8.908213E-4 26.394205 1 ATAACAG 90 8.908213E-4 26.394205 5 >>END_MODULE