FastQCFastQC Report
Thu 26 May 2016
SRR937308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937308_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183698
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14900.811113893455563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.6712103561279926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.43821925116223365No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5450.2966825986129408No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.20468377445590044No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.15242408736077692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTCG250.00501617156.995436
GTATAGA405.3711975E-447.5091251
ACAGTCG405.378417E-447.496195
TAAGGGT405.378417E-447.496193
GGACCGA405.378417E-447.496196
GGTATCA9450.041.727591
GTAAGGT1003.009154E-837.9969524
AGGTAAG651.324468E-436.535532
GGGGCGC651.324468E-436.535536
GTATCAA15300.036.019991
TGCTGTA801.0712771E-535.6318449
GGTAAGG958.6525506E-734.9971923
GTGTAAG902.3988656E-531.6727491
TAAGGTG1102.7186925E-630.2248485
TATAGTT804.481218E-429.685125
GCCTAGG650.00582598729.2363831
ATCAACG19200.028.9429873
TCAACGC19550.028.4248314
TATCAAC19450.028.3267782
CAACGCA19950.027.8549085
Produced by FastQC (version 0.11.5)