##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937308_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 183698 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.361408398567214 33.0 31.0 34.0 31.0 34.0 2 32.56338664547246 34.0 31.0 34.0 31.0 34.0 3 32.60745353787194 34.0 31.0 34.0 31.0 34.0 4 36.234112510751345 37.0 35.0 37.0 35.0 37.0 5 36.181221352437156 37.0 35.0 37.0 35.0 37.0 6 36.2440255201472 37.0 36.0 37.0 35.0 37.0 7 36.188172979564285 37.0 36.0 37.0 35.0 37.0 8 36.166773726442315 37.0 36.0 37.0 35.0 37.0 9 37.89298195952052 39.0 38.0 39.0 35.0 39.0 10-11 37.832303563457415 39.0 38.0 39.0 35.0 39.0 12-13 37.74406090431033 39.0 37.0 39.0 35.0 39.0 14-15 38.93934337880652 40.0 38.0 41.0 36.0 41.0 16-17 38.9277537044497 40.0 38.0 41.0 36.0 41.0 18-19 38.954972291478406 40.0 38.0 41.0 36.0 41.0 20-21 38.90018399764831 40.0 38.0 41.0 35.0 41.0 22-23 38.757607595074525 40.0 38.0 41.0 35.0 41.0 24-25 38.66063865692604 40.0 38.0 41.0 34.5 41.0 26-27 38.589366786791366 40.0 38.0 41.0 34.5 41.0 28-29 38.4471904974469 40.0 38.0 41.0 34.0 41.0 30-31 38.496695663534716 40.0 38.0 41.0 35.0 41.0 32-33 38.38995253078422 40.0 38.0 41.0 35.0 41.0 34-35 38.22732419514638 40.0 38.0 41.0 34.0 41.0 36-37 38.03762153099108 40.0 37.0 41.0 34.0 41.0 38-39 37.86665342028765 40.0 37.0 41.0 33.5 41.0 40-41 37.64887206175353 40.0 37.0 41.0 33.0 41.0 42-43 37.366378512558654 40.0 37.0 41.0 33.0 41.0 44-45 36.94088939454975 40.0 36.5 41.0 31.5 41.0 46-47 36.406466047534536 40.0 36.0 41.0 30.5 41.0 48-49 35.93008089364065 40.0 36.0 41.0 29.5 41.0 50-51 35.61233110866749 39.0 35.0 41.0 27.5 41.0 52-53 35.34092641182811 39.0 35.0 41.0 27.0 41.0 54-55 35.08802491045085 39.0 35.0 40.5 26.0 41.0 56-57 34.796505677797256 39.0 35.0 40.0 25.5 41.0 58-59 34.50238162636501 38.0 34.5 40.0 24.0 41.0 60-61 34.123888120719876 38.0 34.0 40.0 22.5 41.0 62-63 33.744907402366934 37.5 34.0 40.0 21.0 41.0 64-65 33.36874652963016 37.0 33.5 40.0 19.5 41.0 66-67 32.93070419928361 36.5 33.0 39.0 16.0 41.0 68-69 32.451768663785124 36.0 32.5 39.0 10.0 40.0 70-71 32.03246088689044 35.5 32.0 39.0 7.0 40.0 72-73 31.558013696392994 35.0 31.5 38.0 3.5 40.0 74-75 31.075610512907055 35.0 31.0 37.0 2.0 39.5 76-77 29.828664438371675 34.0 29.5 36.0 2.0 38.5 78-79 30.408670753083868 34.5 31.0 36.0 2.0 39.0 80-81 30.182849023941472 34.5 31.0 36.0 2.0 38.0 82-83 29.843093011355595 34.0 30.5 35.5 2.0 37.0 84-85 29.510421996973292 34.0 30.0 35.0 2.0 37.0 86-87 29.11399416433494 34.0 30.0 35.0 2.0 36.0 88-89 28.7079064551601 34.0 29.0 35.0 2.0 36.0 90-91 28.263881479384644 34.0 29.0 35.0 2.0 35.5 92-93 27.93462911953315 34.0 29.0 35.0 2.0 35.0 94-95 27.54163899443652 34.0 27.5 35.0 2.0 35.0 96-97 27.111601650535118 33.0 27.0 35.0 2.0 35.0 98-99 26.397824146152924 33.0 25.0 35.0 2.0 35.0 100-101 24.10036309595096 31.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.05766482304676401 1101 2 -0.15649082459906793 1101 3 0.025576471082892738 1101 4 -0.059735570182930076 1101 5 -0.01686665081675187 1101 6 -0.02274939910832785 1101 7 -0.13284348945969526 1101 8 -0.17578895734926903 1101 9 -0.08211085453913824 1101 10-11 -0.11987267410049895 1101 12-13 -0.10197752009906935 1101 14-15 -0.1933562326014524 1101 16-17 -0.13658151548032293 1101 18-19 -0.10846596836507416 1101 20-21 -0.09530731136780446 1101 22-23 -0.30927818608287794 1101 24-25 -0.38374134800695714 1101 26-27 -0.21633552084004037 1101 28-29 -0.5898999773607727 1101 30-31 -0.4782830758900687 1101 32-33 -0.14819285463507015 1101 34-35 -0.07490878213214813 1101 36-37 -0.09596171872140502 1101 38-39 -0.15544832519706375 1101 40-41 0.03911057633096959 1101 42-43 0.1711427134615633 1101 44-45 0.2872456664333001 1101 46-47 0.21527025094265895 1101 48-49 0.6574856411537411 1101 50-51 0.47055418207602173 1101 52-53 0.6110887646655883 1101 54-55 0.5675030752577257 1101 56-57 0.8470650854205175 1101 58-59 0.8650232635057691 1101 60-61 0.7869292354822477 1101 62-63 0.47694677831055543 1101 64-65 0.4929979459694209 1101 66-67 0.6096487677052025 1101 68-69 0.48701809814664543 1101 70-71 0.9549824847980481 1101 72-73 0.6122247357489599 1101 74-75 0.5979396702874773 1101 76-77 0.6513619947289406 1101 78-79 0.6577616140526743 1101 80-81 0.858716176468878 1101 82-83 0.6624665658880602 1101 84-85 0.5951384049144863 1101 86-87 0.5634645870274895 1101 88-89 0.795197639368098 1101 90-91 0.9188844343250189 1101 92-93 0.837191197846213 1101 94-95 0.7384666630983574 1101 96-97 0.4160085955506503 1101 98-99 0.4721870313292058 1101 100-101 0.7598526841638211 1102 1 0.16086188185596484 1102 2 0.10616613696797828 1102 3 0.10907335566969323 1102 4 0.1143941148459362 1102 5 0.006865747575048431 1102 6 0.03631007003288289 1102 7 -0.06259902003266404 1102 8 -0.007185081660921355 1102 9 0.1517166258823508 1102 10-11 0.014447365532895162 1102 12-13 0.03539124192909071 1102 14-15 0.10613310639742224 1102 16-17 0.006384510319492165 1102 18-19 -0.1465083074973279 1102 20-21 -0.12993889232090794 1102 22-23 -0.30138008962892116 1102 24-25 -0.1279583027143687 1102 26-27 -0.19700947903443478 1102 28-29 -0.5208273606042368 1102 30-31 -0.3871691489450839 1102 32-33 -0.4941847374116861 1102 34-35 -0.5086038336746199 1102 36-37 -0.4312544913438714 1102 38-39 -0.5259157324492065 1102 40-41 -0.3722668745560185 1102 42-43 -0.24145899001207738 1102 44-45 0.19156341952490408 1102 46-47 0.34866614660013084 1102 48-49 0.6522837585676626 1102 50-51 0.5850051262272373 1102 52-53 0.5489853367583493 1102 54-55 0.6444928429831904 1102 56-57 0.6701963138650981 1102 58-59 0.4877248126378291 1102 60-61 0.14164332248515876 1102 62-63 0.3558553356932208 1102 64-65 0.25113422386046835 1102 66-67 0.1299894624328246 1102 68-69 0.14206945006447924 1102 70-71 0.14951002809943859 1102 72-73 0.036140057484445265 1102 74-75 0.13312859580337744 1102 76-77 0.31067203074197636 1102 78-79 0.6008314869590592 1102 80-81 0.42936079071021993 1102 82-83 0.39962858642882537 1102 84-85 0.29768789901711656 1102 86-87 0.5758786035286967 1102 88-89 0.6204010463153722 1102 90-91 0.7844357767156005 1102 92-93 0.9211930270796493 1102 94-95 0.3781981849824696 1102 96-97 0.6532992340674468 1102 98-99 0.9322154225565065 1102 100-101 1.4762643379051745 1103 1 -0.16993309004330115 1103 2 -0.1357790344250489 1103 3 -0.1116997362690455 1103 4 -0.2423135946225088 1103 5 -0.32212734522172326 1103 6 -0.1269101453345698 1103 7 -0.14645075112365902 1103 8 -0.17237878366444193 1103 9 -0.23901227711649398 1103 10-11 -0.3320389702172477 1103 12-13 -0.207851711436561 1103 14-15 -0.3751402215016668 1103 16-17 -0.5879781822027113 1103 18-19 -0.44664988371252434 1103 20-21 -0.4819273946161573 1103 22-23 -0.5549945733047537 1103 24-25 -0.08393024488263734 1103 26-27 -0.30225852446753265 1103 28-29 -0.40861748459067115 1103 30-31 -0.6170052637505208 1103 32-33 -0.9691085865686659 1103 34-35 -0.8699556720203176 1103 36-37 -1.1396503899238226 1103 38-39 -1.2831125233883327 1103 40-41 -1.1993508335418142 1103 42-43 -1.0030854004114929 1103 44-45 -1.573656023608656 1103 46-47 -1.0928907627481905 1103 48-49 -1.0682962748958076 1103 50-51 -0.9205292167304151 1103 52-53 -0.763436903578004 1103 54-55 -0.8944835180790633 1103 56-57 -0.4049785113097286 1103 58-59 -0.35963028625101146 1103 60-61 -0.6510440514905511 1103 62-63 -0.5603143841574791 1103 64-65 -0.6362376067628972 1103 66-67 -0.8760168012421552 1103 68-69 -0.7440796768821976 1103 70-71 -0.6390480395997358 1103 72-73 -0.49665843236925866 1103 74-75 -0.1407892041880423 1103 76-77 -0.018653551651091504 1103 78-79 0.004119916321108974 1103 80-81 -0.518835185093117 1103 82-83 -0.47720010635078935 1103 84-85 -0.11090963841477475 1103 86-87 -0.22432732551158452 1103 88-89 -0.2704565623336457 1103 90-91 -0.5781065888942507 1103 92-93 -0.507010671062698 1103 94-95 -0.38173360102293685 1103 96-97 -0.27523695367119316 1103 98-99 -0.7242155697640555 1103 100-101 -0.09523165076872786 1104 1 -0.1696409486468653 1104 2 -0.13252374457904637 1104 3 -0.2977103368282883 1104 4 -0.14974650643754472 1104 5 -0.12459802674587195 1104 6 -0.13730203215064307 1104 7 -0.5451402882882164 1104 8 -0.3621872223773508 1104 9 -0.39071713083711757 1104 10-11 -0.08960334566067019 1104 12-13 -0.16889256949757225 1104 14-15 -0.5264485976128483 1104 16-17 -0.784610209246658 1104 18-19 -0.7273560312021985 1104 20-21 -0.6345295397686925 1104 22-23 -0.7446986758046421 1104 24-25 -0.3358813320659735 1104 26-27 -0.14007831496041234 1104 28-29 0.05711850305591781 1104 30-31 -0.3061668179427528 1104 32-33 -0.48386172708868 1104 34-35 -0.4656111121104729 1104 36-37 -0.6067218810969806 1104 38-39 -0.9332940683989719 1104 40-41 -1.103987534848109 1104 42-43 -0.647778860617656 1104 44-45 -0.3414036134421323 1104 46-47 -0.45742062377235726 1104 48-49 -0.3245251468126682 1104 50-51 -1.2250657552722188 1104 52-53 -0.9903681668808701 1104 54-55 -1.2559876289258582 1104 56-57 -1.3675635453233284 1104 58-59 -1.2586954337824139 1104 60-61 -1.2436737414033345 1104 62-63 -1.058206792654616 1104 64-65 -0.9524598011621279 1104 66-67 -1.3611384572665273 1104 68-69 -1.2680561386325415 1104 70-71 -1.2042790399753436 1104 72-73 -1.0371200157756277 1104 74-75 -0.6868014741266926 1104 76-77 -0.7861716018159406 1104 78-79 -0.7151739361892204 1104 80-81 -0.7632323304543291 1104 82-83 -0.8512871644869264 1104 84-85 -0.42149767731131504 1104 86-87 -0.18457522835370455 1104 88-89 -0.26423812403808355 1104 90-91 -0.3966450472223677 1104 92-93 -0.20846303114783993 1104 94-95 0.3579684147526976 1104 96-97 -0.5709883830773101 1104 98-99 -0.5718638315227444 1104 100-101 0.45428631592715263 1105 1 -0.2447325237384419 1105 2 0.11315914396098492 1105 3 -0.19599657440024032 1105 4 0.2149185903704094 1105 5 0.18518742589672854 1105 6 0.0022191609419763836 1105 7 -0.1517598591935041 1105 8 -0.13043682991267502 1105 9 -0.10964701048128944 1105 10-11 -0.16037780929228518 1105 12-13 -0.23646190492405594 1105 14-15 -0.396052208287891 1105 16-17 -0.15401758758261508 1105 18-19 0.065030154041132 1105 20-21 0.21577789089587895 1105 22-23 -0.17506890081773463 1105 24-25 -0.49509116984723534 1105 26-27 -0.47017381469876796 1105 28-29 -0.5697784095552834 1105 30-31 -0.2612950230709501 1105 32-33 -0.5763525695795195 1105 34-35 -0.35169823926901955 1105 36-37 -0.6200656801550579 1105 38-39 -0.7855311170645862 1105 40-41 -1.162039601828745 1105 42-43 -0.7314065424596237 1105 44-45 -0.40939811893664313 1105 46-47 0.00863118156516407 1105 48-49 -0.05313582185191734 1105 50-51 -0.7524074611853493 1105 52-53 -0.5030251527521372 1105 54-55 -0.3952798842895433 1105 56-57 -1.0636323574635753 1105 58-59 -0.2989884740754505 1105 60-61 -0.5476049292630876 1105 62-63 0.1150336573715478 1105 64-65 0.04309226581851533 1105 66-67 -0.06800004805669602 1105 68-69 -0.04062285762782736 1105 70-71 -0.44839206980266155 1105 72-73 0.6178708267152118 1105 74-75 0.22666006433484753 1105 76-77 0.42474545731540303 1105 78-79 -0.13736781374024076 1105 80-81 0.43810204945148 1105 82-83 0.21693627873651877 1105 84-85 -0.03622818489896673 1105 86-87 0.3167002818503768 1105 88-89 -0.08720384878952459 1105 90-91 0.00952318930301388 1105 92-93 0.18998673337335603 1105 94-95 0.7405233598076428 1105 96-97 1.0693831501513635 1105 98-99 1.0226874505285366 1105 100-101 1.543572030212868 1106 1 -0.34848923214631355 1106 2 -0.40115212616424145 1106 3 -0.4855761808403116 1106 4 -0.4852155777870166 1106 5 -0.14128842383493634 1106 6 -0.07939980149094339 1106 7 -0.04308365168008521 1106 8 0.1703234563127296 1106 9 -0.024003003325653083 1106 10-11 -0.1161023173423743 1106 12-13 -0.07523650241957824 1106 14-15 -0.31857009737554876 1106 16-17 -0.344648177922501 1106 18-19 -0.23103424667443306 1106 20-21 -0.17364518602720125 1106 22-23 -1.0235706897264976 1106 24-25 -0.2531412592926827 1106 26-27 -0.1149636179188036 1106 28-29 -0.36852617341931904 1106 30-31 -0.4146939497256952 1106 32-33 -0.09155829408756944 1106 34-35 -0.15972596735489475 1106 36-37 -0.0511926926774251 1106 38-39 -0.30230213674258977 1106 40-41 0.19920816203529768 1106 42-43 0.09518111406988794 1106 44-45 0.31723426031201996 1106 46-47 0.2177098935508539 1106 48-49 0.3446727649101575 1106 50-51 0.1492025567037416 1106 52-53 0.15652314778453302 1106 54-55 0.13904480265141927 1106 56-57 -1.2770715345655432 1106 58-59 -1.1961262200504095 1106 60-61 -0.3864913335564708 1106 62-63 -0.05860229969464115 1106 64-65 -0.714957823626932 1106 66-67 -0.8084964702391559 1106 68-69 -0.30649942292300025 1106 70-71 -0.3406103166720733 1106 72-73 -0.702679262730225 1106 74-75 -1.285638683429017 1106 76-77 -0.9765291401801264 1106 78-79 -0.7173096742053566 1106 80-81 -0.3315759469707018 1106 82-83 -0.44071130623665056 1106 84-85 -0.0974983101225817 1106 86-87 -0.4783578576845038 1106 88-89 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0.4866900300766517 2316 94-95 0.7954684147526976 2316 96-97 1.3303721611403745 2316 98-99 1.9876599780010622 2316 100-101 2.0078577444985832 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 2.0 6 4.0 7 20.0 8 48.0 9 88.0 10 142.0 11 252.0 12 456.0 13 667.0 14 867.0 15 1405.0 16 2008.0 17 3114.0 18 2640.0 19 1416.0 20 1159.0 21 1156.0 22 1151.0 23 1235.0 24 1401.0 25 1532.0 26 1606.0 27 1937.0 28 2180.0 29 2753.0 30 3251.0 31 4132.0 32 5610.0 33 7506.0 34 10733.0 35 16197.0 36 25870.0 37 40848.0 38 36469.0 39 3842.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88439835290529 20.45305997952025 15.32440793917079 20.33813372840367 2 15.652320656730067 21.96648847565025 39.855632614399724 22.52555825321996 3 19.452035405938005 26.544654813879305 30.927391697242214 23.07591808294048 4 12.677329094492046 18.089472939280775 38.11473178804342 31.118466178183755 5 12.71346525490623 38.898173602983206 33.74887721494869 14.639483927161873 6 30.077083038465307 37.91984670491785 17.387777765680628 14.615292490936211 7 27.25941490925323 35.209419808598895 20.588683600256942 16.942481681890932 8 27.01444762599484 34.430968219577785 19.39650948839944 19.15807466602794 9 27.087937810972356 17.057344119152088 20.749273263726334 35.10544480614922 10-11 24.53864494986336 28.101285806051234 26.86882818539124 20.49124105869416 12-13 26.118626997305167 25.82682309388355 27.94920652203501 20.105343386776276 14-15 22.759910287537153 26.555814434561075 27.349780618188547 23.334494659713226 16-17 20.762338185500116 30.258358828076513 27.615706213459045 21.36359677296432 18-19 20.459939683611143 27.556097507866173 31.966869535868653 20.01709327265403 20-21 21.694574791233435 26.627943690187156 31.841936221406876 19.835545297172533 22-23 21.26943773799576 26.878448301673146 31.699470046898266 20.152643913432826 24-25 21.290436723417574 26.892581553913363 31.956069080961907 19.860912641707156 26-27 20.947266422423883 27.220096136572725 32.01739821553972 19.81523922546367 28-29 20.353363238464386 27.77938070248024 31.9080308342225 19.95922522483287 30-31 20.57268062983982 27.344411328406753 31.940554444126896 20.142353597626535 32-33 20.591133273089525 27.657895023353547 31.543348321701924 20.207623381855 34-35 20.840183344402224 27.43361386623698 31.771440081002517 19.954762708358285 36-37 21.510577142919356 27.040849655412693 31.464414419318665 19.98415878234929 38-39 20.789613358918874 27.62558009771499 31.45606227629663 20.128744267069504 40-41 20.692930788576902 27.96111008285338 31.24693790895927 20.099021219610446 42-43 20.88668931573675 28.18720327540398 30.65763970556209 20.268467703297183 44-45 21.06718636022167 27.89088612831931 30.486450587377107 20.555476924081916 46-47 21.1825931692234 28.485067883156052 29.93962917397032 20.392709773650232 48-49 21.49533473418328 28.610273383488117 29.277673137432092 20.616718744896513 50-51 21.342094089211642 28.688390728260515 29.251271108014244 20.718244074513603 52-53 21.314875502182932 29.240383673202757 28.70934904027262 20.73539178434169 54-55 21.033707498176355 28.93471894087034 28.843808860194446 21.187764700758855 56-57 21.253633681368335 28.8617731276334 28.94070703001666 20.943886160981613 58-59 21.529902339709743 28.24037278576795 28.96465938660192 21.26506548792039 60-61 20.836372742218206 28.220503217236985 29.74147786040131 21.201646180143495 62-63 20.65754662543958 28.031878409128026 30.05775784167492 21.252817123757474 64-65 20.475726464087796 27.950222648041894 29.9358185717863 21.638232316084007 66-67 20.44551383248593 27.77765680627987 29.804897168178208 21.97193219305599 68-69 20.427821750917268 27.710426896318957 29.502226480418948 22.359524872344828 70-71 20.399786606277694 27.887075526135284 29.52998943918823 22.183148428398784 72-73 20.27430892007534 27.402584677024244 29.92248146414223 22.400624938758178 74-75 19.83010157976679 27.738734226828814 29.181863711091026 23.249300482313362 76-77 19.74191335779377 27.03241189343379 29.838920401964092 23.386754346808345 78-79 19.926727563718714 27.334810395322755 29.38654748554693 23.3519145554116 80-81 19.594932987838735 27.238184411370835 28.991061416019768 24.17582118477066 82-83 19.50511165064399 26.88298185064617 29.040871430282312 24.571035068427527 84-85 19.14092695619985 26.843242713584253 29.349802393058173 24.666027937157725 86-87 19.299883504447518 26.754510119870655 28.712070898975494 25.233535476706333 88-89 18.58457903733301 26.557719735653084 28.7646027719409 26.093098455073 90-91 18.708999548144398 26.69377800279824 28.861323882473993 25.73589856658337 92-93 18.426962010501075 27.052105250264702 28.503964441153972 26.016968298080243 94-95 18.024148330411872 27.453483434767932 27.670143387516465 26.85222484730373 96-97 18.198347287395617 28.31141329791288 27.14373591438121 26.34650350031029 98-99 18.4988908395596 28.485145415696998 26.411627812027927 26.60433593271547 100-101 17.849569494690478 27.50579133762886 25.96234201421498 28.682297153465687 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 107.0 1 70.0 2 42.0 3 58.5 4 57.0 5 49.5 6 79.0 7 150.5 8 240.5 9 321.5 10 331.0 11 315.0 12 355.5 13 406.0 14 432.0 15 444.0 16 490.5 17 533.5 18 545.0 19 555.0 20 586.0 21 665.5 22 750.5 23 824.5 24 927.0 25 1109.0 26 1341.0 27 1626.5 28 1868.0 29 2203.5 30 2636.5 31 2990.5 32 3341.5 33 3732.0 34 4083.5 35 4365.0 36 4753.5 37 5121.5 38 5433.5 39 5575.5 40 5638.0 41 5988.5 42 6375.5 43 6705.0 44 7050.5 45 7170.5 46 7068.0 47 7391.5 48 7896.0 49 7535.5 50 6810.5 51 6248.5 52 5833.5 53 5528.5 54 5119.5 55 4604.5 56 3972.0 57 3437.0 58 3068.5 59 2667.0 60 2146.0 61 1639.0 62 1237.0 63 898.5 64 609.5 65 421.0 66 313.0 67 249.0 68 177.5 69 112.0 70 81.5 71 49.0 72 41.5 73 37.5 74 23.5 75 20.0 76 16.0 77 12.0 78 12.5 79 7.5 80 6.5 81 5.0 82 2.0 83 3.5 84 3.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05552591753856874 2 0.0 3 0.0 4 0.0 5 0.0016331152217226098 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007076832627464643 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013609293514355084 24-25 2.721858702871017E-4 26-27 5.443717405742034E-4 28-29 0.0032662304434452195 30-31 2.721858702871017E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.721858702871017E-4 40-41 0.0 42-43 0.014153665254929285 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.005988089146316236 92-93 0.0013609293514355084 94-95 0.0 96-97 0.0 98-99 2.721858702871017E-4 100-101 0.01007087720062276 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 183698.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.41116717689292 #Duplication Level Percentage of deduplicated Percentage of total 1 83.96136873988496 56.59933864525137 2 9.726566074214414 13.113583433719258 3 2.696638283785784 5.453506024916792 4 1.139757146300736 3.073294381213493 5 0.6276932159360291 2.11567661576326 6 0.4264744531641382 1.7249484395353027 7 0.2914728225188184 1.3753966216435833 8 0.18140611183881186 0.9783038185661024 9 0.12886347673712142 0.781815363598957 >10 0.7622675043039399 8.690067160677296 >50 0.03964977332084341 1.855617432887419 >100 0.01387742066229519 1.5168352800340084 >500 0.0019824886660421703 0.9020826555268332 >1k 0.0019824886660421703 1.8195341266663456 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1490 0.811113893455563 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1233 0.6712103561279926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 805 0.43821925116223365 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 545 0.2966825986129408 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 376 0.20468377445590044 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 280 0.15242408736077692 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 5.443717405742033E-4 0.0 26-27 0.0 0.0 0.0 0.003810602184019423 0.0 28-29 0.0 0.0 0.0 0.00816557610861305 0.0 30-31 0.0 0.0 0.0 0.015786780476651895 0.0 32-33 0.0 0.0 0.0 0.02803514463957147 0.0 34-35 0.0 0.0 0.0 0.03756165009962003 0.0 36-37 0.0 0.0 0.0 0.0498100142625396 0.0 38-39 0.0 0.0 0.0 0.0827445045672789 0.0 40-41 0.0 0.0 0.0 0.14235321016015418 0.0 42-43 0.0 0.0 0.0 0.2593931343836079 0.0 44-45 0.0 0.0 0.0 0.37507212925562605 0.0 46-47 0.0 0.0 0.0 0.6355540071203823 0.0 48-49 0.0 0.0 0.0 0.9338697209550457 0.0 50-51 0.0 0.0 0.0 1.1369203801892236 0.0 52-53 0.0 0.0 0.0 1.4012128602379994 0.0 54-55 0.0 0.0 0.0 1.6630556674541912 0.0 56-57 0.0 0.0 0.0 2.281461964746486 0.0 58-59 0.0 0.0 0.0 2.986423368790079 0.0 60-61 0.0 0.0 0.0 3.7289464229332925 0.0 62-63 0.0 0.0 0.0 4.625526679659005 0.0 64-65 0.0 0.0 0.0 5.5520473821162994 0.0 66-67 0.0 0.0 0.0 6.6884233905649495 0.0 68-69 0.0 0.0 0.0 7.8492961273394375 0.0 70-71 0.0 0.0 0.0 9.03248810547747 0.0 72-73 0.0 0.0 0.0 10.183834336791909 0.0 74-75 0.0 0.0 0.0 11.41574758571133 0.0 76-77 0.0 0.0 0.0 13.01701706061035 0.0 78-79 0.0 0.0 0.0 14.368964278326384 0.0 80-81 0.0 0.0 0.0 15.929950244422912 0.0 82-83 0.0 0.0 0.0 17.782991649337497 0.0 84-85 0.0 0.0 0.0 20.121884832714564 0.0 86-87 0.0 0.0 0.0 22.017115047523653 0.0 88-89 0.0 0.0 0.0 24.041633550719116 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGACTCG 25 0.005016171 56.99543 6 GTATAGA 40 5.3711975E-4 47.509125 1 ACAGTCG 40 5.378417E-4 47.49619 5 TAAGGGT 40 5.378417E-4 47.49619 3 GGACCGA 40 5.378417E-4 47.49619 6 GGTATCA 945 0.0 41.72759 1 GTAAGGT 100 3.009154E-8 37.996952 4 AGGTAAG 65 1.324468E-4 36.53553 2 GGGGCGC 65 1.324468E-4 36.53553 6 GTATCAA 1530 0.0 36.01999 1 TGCTGTA 80 1.0712771E-5 35.631844 9 GGTAAGG 95 8.6525506E-7 34.997192 3 GTGTAAG 90 2.3988656E-5 31.672749 1 TAAGGTG 110 2.7186925E-6 30.224848 5 TATAGTT 80 4.481218E-4 29.68512 5 GCCTAGG 65 0.005825987 29.236383 1 ATCAACG 1920 0.0 28.942987 3 TCAACGC 1955 0.0 28.424831 4 TATCAAC 1945 0.0 28.326778 2 CAACGCA 1995 0.0 27.854908 5 >>END_MODULE