FastQCFastQC Report
Thu 26 May 2016
SRR937307_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937307_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155844
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.5556838890172224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.4241420908087575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.27335027335027334No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2290.14694181360848027No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2280.1463001463001463No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2080.13346680013346682No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1810.11614178280844947No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1790.11485844819178154No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.10523343856677189No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACA405.362876E-447.5194661
GTATATA601.4797188E-647.5194631
AGGTAAG500.001610822938.0033452
AAGGTAA702.0402834E-433.9424741
TAAGGTG1051.886423E-631.6694555
GTATCAA9250.029.7959881
AATTGAA804.473674E-429.6901152
GTCATAC650.00581707729.242751
ACTGGAC650.005826234429.2333413
GGTAAGG1004.923021E-528.502513
CTACTCC700.00836606327.1452474
CCCTGTA700.00836606327.1452472
TTATACT1109.419406E-525.9113714
GTATTTA950.001218871725.0102441
TTTATGG950.001221161525.00223
GTACAAA1151.2712546E-424.7927651
GTAAGGT1151.2740368E-424.784794
CAACGCA10850.024.5182885
ATCAACG10900.024.4058173
AACGCAG10950.024.2943766