##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937307_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 155844 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97335155668489 33.0 31.0 34.0 31.0 34.0 2 32.11178486178486 33.0 31.0 34.0 31.0 34.0 3 32.14012089012089 34.0 31.0 34.0 30.0 34.0 4 35.74559816226483 37.0 35.0 37.0 35.0 37.0 5 35.73521598521599 37.0 35.0 37.0 35.0 37.0 6 35.78834603834604 37.0 35.0 37.0 35.0 37.0 7 35.732739149405816 37.0 35.0 37.0 35.0 37.0 8 35.70933112599779 37.0 35.0 37.0 35.0 37.0 9 37.38074613074613 39.0 37.0 39.0 35.0 39.0 10-11 37.28332178332178 39.0 37.0 39.0 35.0 39.0 12-13 37.15725340725341 39.0 37.0 39.0 34.0 39.0 14-15 38.33562408562409 40.0 38.0 41.0 34.0 41.0 16-17 38.273103231436565 40.0 38.0 41.0 34.0 41.0 18-19 38.21848771848772 40.0 38.0 41.0 34.0 41.0 20-21 38.095274761941425 40.0 38.0 41.0 34.0 41.0 22-23 38.13542067708734 40.0 38.0 41.0 34.0 41.0 24-25 38.09505017838352 40.0 38.0 41.0 34.0 41.0 26-27 38.00617283950618 40.0 38.0 41.0 34.0 41.0 28-29 37.867899951233284 40.0 38.0 41.0 33.0 41.0 30-31 37.59720297220297 40.0 37.0 41.0 33.0 41.0 32-33 37.32914003747337 40.0 36.5 41.0 32.5 41.0 34-35 37.335977002643666 40.0 37.0 41.0 33.0 41.0 36-37 37.209684043017376 40.0 37.0 41.0 32.5 41.0 38-39 37.05153550986884 40.0 36.5 41.0 32.0 41.0 40-41 36.80569351402684 39.5 36.0 41.0 31.0 41.0 42-43 36.49815520648854 39.0 36.0 41.0 30.5 41.0 44-45 36.20930225096892 39.0 35.5 41.0 30.0 41.0 46-47 35.730907189240526 39.0 35.0 40.5 28.5 41.0 48-49 35.316540899874234 39.0 35.0 40.0 26.5 41.0 50-51 34.30344447011114 38.0 34.0 39.5 25.0 40.5 52-53 34.37951733785067 38.0 34.5 39.5 24.5 40.5 54-55 34.631060547727216 38.0 35.0 40.0 24.0 41.0 56-57 34.34360321860322 38.0 34.0 40.0 23.0 41.0 58-59 34.03765303765304 38.0 34.0 40.0 22.0 41.0 60-61 33.66638112471446 37.5 34.0 40.0 20.5 41.0 62-63 33.27846115346115 37.0 33.0 40.0 18.0 41.0 64-65 32.8443411360078 36.5 33.0 39.5 14.0 41.0 66-67 32.371611996611996 36.0 32.0 39.0 8.0 40.5 68-69 31.822235697235698 35.5 31.5 39.0 4.5 40.0 70-71 31.315029773363108 35.0 31.0 38.0 2.0 40.0 72-73 30.8409916326583 35.0 31.0 37.5 2.0 40.0 74-75 30.287367495700828 35.0 30.0 37.0 2.0 39.0 76-77 29.77728369395036 34.0 29.5 36.0 2.0 39.0 78-79 29.297383922383922 34.0 29.0 36.0 2.0 38.5 80-81 28.783594491927825 34.0 29.0 35.0 2.0 37.0 82-83 28.236390236390235 34.0 27.5 35.0 2.0 37.0 84-85 27.752913169579834 34.0 26.5 35.0 2.0 36.0 86-87 27.231856856856858 33.0 26.0 35.0 2.0 36.0 88-89 26.712991837991837 33.0 25.0 35.0 2.0 35.5 90-91 26.188014938014938 33.0 24.5 35.0 2.0 35.0 92-93 25.534094992428326 32.0 21.5 34.5 2.0 35.0 94-95 24.814198814198814 32.0 18.0 34.0 2.0 35.0 96-97 24.01742768409435 31.5 4.5 34.0 2.0 35.0 98-99 22.783478991812323 31.0 2.0 34.0 2.0 35.0 100-101 19.721801930135264 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12543766274826496 1101 2 0.3026588269392576 1101 3 0.26690486221572485 1101 4 0.2487866157523797 1101 5 0.13231689511316347 1101 6 0.29239985746997377 1101 7 0.24212837732789438 1101 8 0.5177115965885477 1101 9 0.11613041380445566 1101 10-11 0.3271764482315831 1101 12-13 0.5182156331774976 1101 14-15 0.6808621062645202 1101 16-17 0.3898003445603635 1101 18-19 0.6988662432416461 1101 20-21 0.32878326442697414 1101 22-23 0.49297605796451904 1101 24-25 0.5159113856803828 1101 26-27 0.3113272392713142 1101 28-29 0.2554749702825063 1101 30-31 0.3862254943863803 1101 32-33 0.4482400885094364 1101 34-35 0.06982077076951754 1101 36-37 0.29734861541298585 1101 38-39 0.46350616489031893 1101 40-41 0.6005884436402056 1101 42-43 0.5600285368188196 1101 44-45 0.628582961280955 1101 46-47 1.211179365029487 1101 48-49 1.112130372328771 1101 50-51 1.4709243275880155 1101 52-53 1.5132879501761636 1101 54-55 1.8426424587012704 1101 56-57 1.3373809208851029 1101 58-59 1.2509307599912773 1101 60-61 1.4194081247691557 1101 62-63 1.7764065876991992 1101 64-65 1.8049488337429977 1101 66-67 2.2783436640423815 1101 68-69 1.9860467213737465 1101 70-71 1.4414729925706204 1101 72-73 1.9512411242952474 1101 74-75 2.2322368193967 1101 76-77 2.054027850800356 1101 78-79 2.1259787021017296 1101 80-81 2.109713338305344 1101 82-83 1.863742175388282 1101 84-85 2.3081735973181843 1101 86-87 2.79855492868575 1101 88-89 1.7712138992857476 1101 90-91 1.3300587059876499 1101 92-93 2.061771798799178 1101 94-95 1.9855920396024302 1101 96-97 1.7743053351526683 1101 98-99 2.310769127400004 1101 100-101 1.0900719674632242 1102 1 0.34551122282865165 1102 2 0.9103257590288933 1102 3 0.6184765453765309 1102 4 0.5595969141636346 1102 5 0.35159418628215633 1102 6 0.49268044746732187 1102 7 0.4254218972156565 1102 8 0.5933571431702802 1102 9 0.9265804953272294 1102 10-11 0.6141745144356534 1102 12-13 0.6536951008148506 1102 14-15 0.4973221221898996 1102 16-17 0.4839307051564177 1102 18-19 0.7763318330494045 1102 20-21 0.3661699856949383 1102 22-23 0.43894352469185094 1102 24-25 0.2960084546524371 1102 26-27 0.3802613764570708 1102 28-29 0.2678360971926068 1102 30-31 0.24221457412701852 1102 32-33 0.2843603630326186 1102 34-35 0.5666925714747819 1102 36-37 0.5549416082465655 1102 38-39 0.16809039876587661 1102 40-41 0.451382816673231 1102 42-43 0.27504010167681514 1102 44-45 0.546794768811381 1102 46-47 0.7864267771555191 1102 48-49 1.1605131880873074 1102 50-51 0.8073100250816552 1102 52-53 0.9459653095967795 1102 54-55 1.1472911524410776 1102 56-57 1.1993230586775354 1102 58-59 1.1413489907575922 1102 60-61 1.2297444535903068 1102 62-63 1.3792200711860971 1102 64-65 1.1446333595838212 1102 66-67 1.492805424175856 1102 68-69 1.6836162053156585 1102 70-71 1.7718108380944493 1102 72-73 1.7097441387418364 1102 74-75 1.8657824911063976 1102 76-77 1.9195721574776385 1102 78-79 1.3705470105990578 1102 80-81 1.5368926504806737 1102 82-83 1.8148474724724188 1102 84-85 1.8216343336773413 1102 86-87 1.4370488429162158 1102 88-89 1.658181631436051 1102 90-91 1.4328532306909452 1102 92-93 1.431979966243155 1102 94-95 1.8917460607604326 1102 96-97 2.278135009440767 1102 98-99 2.2362800666181393 1102 100-101 2.333900883399977 1103 1 0.7004948603016068 1103 2 0.31162366795340546 1103 3 0.4798665281820149 1103 4 0.27045164820323464 1103 5 0.4657436122070564 1103 6 0.2732561865543488 1103 7 0.02412398829114437 1103 8 0.29078905841793556 1103 9 0.3269909451580588 1103 10-11 0.3149898674779763 1103 12-13 0.21964440471412416 1103 14-15 -0.03975702806802417 1103 16-17 0.16708007561512517 1103 18-19 0.4020459602888522 1103 20-21 0.11920675978112882 1103 22-23 0.24006948841696385 1103 24-25 0.404084332404949 1103 26-27 0.5513468498610266 1103 28-29 0.579306505978451 1103 30-31 0.4984961205244929 1103 32-33 0.37934621915331945 1103 34-35 0.7398025609362051 1103 36-37 1.0391892093336992 1103 38-39 1.1119863441871374 1103 40-41 0.9356026846636922 1103 42-43 1.0854289196922338 1103 44-45 1.0428566690831644 1103 46-47 1.0245986114225403 1103 48-49 0.6486434227093056 1103 50-51 1.1960725743079053 1103 52-53 1.1326090502368586 1103 54-55 0.35326486146542635 1103 56-57 -0.26295992984096017 1103 58-59 -0.2792788865511895 1103 60-61 0.5142860255022228 1103 62-63 0.48416210901404355 1103 64-65 0.7200396494501504 1103 66-67 0.500737089825634 1103 68-69 0.0965897479414295 1103 70-71 -0.39372801597399487 1103 72-73 -0.2674869888858744 1103 74-75 -0.6000226006901492 1103 76-77 -0.31843327820930867 1103 78-79 -0.15186133151642167 1103 80-81 0.08739461953054217 1103 82-83 0.2433238161409541 1103 84-85 0.258680204219246 1103 86-87 0.3586473786087083 1103 88-89 0.2407707933418699 1103 90-91 0.5336353040172526 1103 92-93 0.750383649198394 1103 94-95 1.0974424388180068 1103 96-97 0.1515056900109606 1103 98-99 -0.4098733528726761 1103 100-101 0.7029729339851478 1104 1 0.4835527115412752 1104 2 0.13393771754017791 1104 3 -0.10071198421467997 1104 4 0.45089241955309944 1104 5 0.4719419593144991 1104 6 -0.004980728046206195 1104 7 0.3912038780983096 1104 8 0.6075934661314335 1104 9 0.4819496228440059 1104 10-11 0.6651964790482197 1104 12-13 0.6425094184882383 1104 14-15 0.7105873245490599 1104 16-17 0.6870525274057613 1104 18-19 0.3824178611152931 1104 20-21 0.47182383967093955 1104 22-23 0.33786563166764694 1104 24-25 0.538381853066106 1104 26-27 0.6928757754808643 1104 28-29 0.5906701423420841 1104 30-31 0.34250438498730773 1104 32-33 0.202348973974253 1104 34-35 0.8007529741593444 1104 36-37 0.748555600518273 1104 38-39 0.6075097601651009 1104 40-41 0.9335365689612161 1104 42-43 0.9494096359456847 1104 44-45 1.038380085061128 1104 46-47 1.2312101816704697 1104 48-49 1.3297728992933244 1104 50-51 1.9508935109470187 1104 52-53 1.713531915250634 1104 54-55 1.62840260251226 1104 56-57 1.3875221638229505 1104 58-59 1.5488891575259416 1104 60-61 1.5618066866592457 1104 62-63 1.7455257453776767 1104 64-65 0.8739652692848594 1104 66-67 1.5840704231589697 1104 68-69 1.890322530310577 1104 70-71 1.796698981271188 1104 72-73 2.1140557108386453 1104 74-75 1.7061068758938696 1104 76-77 2.133701708016588 1104 78-79 2.082642800714982 1104 80-81 2.549171479034669 1104 82-83 1.8717673423117525 1104 84-85 1.7879501766710355 1104 86-87 2.120011014972345 1104 88-89 2.322382363589803 1104 90-91 1.6975471497472796 1104 92-93 1.919116431567538 1104 94-95 2.0158308685700703 1104 96-97 1.690073183123907 1104 98-99 2.6665729281190558 1104 100-101 2.01116852627165 1105 1 -0.12861737643526183 1105 2 0.037896661821704924 1105 3 -0.06478823054899152 1105 4 0.06257374897636225 1105 5 0.2534668860007159 1105 6 -0.08904719920945325 1105 7 -0.43601997331909104 1105 8 0.08291115919106318 1105 9 0.36098188090852545 1105 10-11 0.03726392772857423 1105 12-13 0.2943911389183427 1105 14-15 0.294653795712307 1105 16-17 0.1365882067801536 1105 18-19 0.07523311038215752 1105 20-21 0.2732656774030957 1105 22-23 -0.25463192453958783 1105 24-25 0.39175428708369964 1105 26-27 0.58217196316415 1105 28-29 0.5030601716675989 1105 30-31 0.47226978088173155 1105 32-33 0.5046461391746391 1105 34-35 0.9176884580303124 1105 36-37 0.9669084841937448 1105 38-39 0.3823142567437898 1105 40-41 0.07026677423980487 1105 42-43 -0.4038406084335975 1105 44-45 0.2636977781207577 1105 46-47 0.6817233781514105 1105 48-49 0.6282821857058352 1105 50-51 0.34043407790694147 1105 52-53 0.38997375298279024 1105 54-55 0.5555775780743275 1105 56-57 0.9130597982315578 1105 58-59 -0.0996934426695617 1105 60-61 0.19193865146863232 1105 62-63 0.45337032015773815 1105 64-65 0.16612069450479794 1105 66-67 0.4153265326408899 1105 68-69 -0.14767844720653045 1105 70-71 0.3598710242819365 1105 72-73 0.5969491614740328 1105 74-75 0.035407951162689244 1105 76-77 0.3634182280556857 1105 78-79 0.6160005719759809 1105 80-81 0.2570160538147306 1105 82-83 1.0023880656744133 1105 84-85 1.7088934806788565 1105 86-87 1.5892192261160396 1105 88-89 1.261531923707107 1105 90-91 0.774404432249721 1105 92-93 0.8976110552234573 1105 94-95 1.2845258832328277 1105 96-97 2.025239361032021 1105 98-99 1.915106652459233 1105 100-101 2.076539982772136 1106 1 -0.03222268952823981 1106 2 -0.2377200364705132 1106 3 0.2968815986730178 1106 4 0.20365534290425558 1106 5 0.14654088979578006 1106 6 -0.1607739544276683 1106 7 -0.20300289552022832 1106 8 0.08281628253072171 1106 9 0.2848907993145957 1106 10-11 -0.017290151967827683 1106 12-13 -0.5653337187666736 1106 14-15 -0.017442978481241767 1106 16-17 0.3243965559262634 1106 18-19 0.42907561512598846 1106 20-21 0.634034178351861 1106 22-23 0.4826071646444774 1106 24-25 0.6846385375580866 1106 26-27 0.7881966310958362 1106 28-29 0.7140658642672193 1106 30-31 0.834081925094253 1106 32-33 0.8093899721917239 1106 34-35 0.6434000329828748 1106 36-37 1.0104094329603441 1106 38-39 0.5149518279012852 1106 40-41 0.6370125047900927 1106 42-43 -0.2730056938225971 1106 44-45 -0.2584559220689968 1106 46-47 -0.7671855402546655 1106 48-49 -0.2831504518653176 1106 50-51 -0.9663970416376415 1106 52-53 -0.7316106872449097 1106 54-55 -0.3354565775233951 1106 56-57 -0.5090464636280245 1106 58-59 -0.22308053944375672 1106 60-61 -0.8662681796509162 1106 62-63 -1.3238592813602885 1106 64-65 -0.5831202922124703 1106 66-67 -0.32760515616366703 1106 68-69 -0.680221141703683 1106 70-71 -0.5214382736307748 1106 72-73 -0.4178515975392507 1106 74-75 -0.8532068495963223 1106 76-77 -1.0607278820012382 1106 78-79 -1.4645497126540015 1106 80-81 -1.2349194300562374 1106 82-83 -1.2918244580447542 1106 84-85 -1.1932886825089994 1106 86-87 -1.2424221401078697 1106 88-89 -0.6109064064636698 1106 90-91 -0.6106524937464819 1106 92-93 -1.397550235099125 1106 94-95 -1.3246771528203034 1106 96-97 -0.9910319862165551 1106 98-99 -0.17540568150661429 1106 100-101 0.09864624463171978 1107 1 0.24004621803478798 1107 2 0.4567577546459347 1107 3 0.3042045278446892 1107 4 0.29381140532922245 1107 5 0.42973416710670165 1107 6 0.2878454933514476 1107 7 0.3687858199659644 1107 8 0.23251616248270324 1107 9 -0.20682588736007546 1107 10-11 -0.04189999590539628 1107 12-13 -0.19762663593353835 1107 14-15 0.1907202868248774 1107 16-17 0.11083112975578757 1107 18-19 -0.014381767827188696 1107 20-21 -0.007922604361219499 1107 22-23 0.504996120226707 1107 24-25 0.372333615589298 1107 26-27 0.33225425414506304 1107 28-29 0.3988797898374443 1107 30-31 0.17807952413387795 1107 32-33 0.3152123011232959 1107 34-35 0.3594264435471004 1107 36-37 0.505667536201635 1107 38-39 0.4386012876852021 1107 40-41 0.6751877748981343 1107 42-43 0.5816761789265144 1107 44-45 0.844425230391991 1107 46-47 0.6041229831547028 1107 48-49 -0.48207001969245056 1107 50-51 -0.7808628279521699 1107 52-53 -0.23815330429172832 1107 54-55 -0.0412789064549699 1107 56-57 0.1179643406937032 1107 58-59 0.34426639933175807 1107 60-61 -0.004983665845394114 1107 62-63 -0.2579529188152705 1107 64-65 -0.2348009644813729 1107 66-67 0.44322317516886756 1107 68-69 0.2076540083563394 1107 70-71 0.17850170235961826 1107 72-73 0.8383908994595473 1107 74-75 1.332807556165978 1107 76-77 1.2082997290431798 1107 78-79 0.9265667339246306 1107 80-81 1.3263050597396813 1107 82-83 1.1775032730476873 1107 84-85 0.9559310053661498 1107 86-87 0.8498347626532343 1107 88-89 1.173727447077745 1107 90-91 1.211616413816543 1107 92-93 1.1197967036763856 1107 94-95 1.2962112025218318 1107 96-97 2.269232119425702 1107 98-99 2.6382797926830612 1107 100-101 2.835921154595706 1108 1 0.5754499447428607 1108 2 0.3019219072634982 1108 3 0.7066002687497459 1108 4 -0.24937108505823602 1108 5 0.43076936379407726 1108 6 0.45746986483916885 1108 7 0.7874489373868485 1108 8 0.24922719472168353 1108 9 0.369630782264295 1108 10-11 0.4878249375462431 1108 12-13 0.4541036213867855 1108 14-15 0.7753106447071616 1108 16-17 -0.0787577492740823 1108 18-19 0.18363656994269917 1108 20-21 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-1.1853084509319203 2316 80-81 -0.34723827063594825 2316 82-83 -0.4762234350268493 2316 84-85 -1.4730840789284372 2316 86-87 -1.592933649059276 2316 88-89 -1.7634213254747522 2316 90-91 -1.5942842073014667 2316 92-93 -0.9975502350991263 2316 94-95 -1.129920119572219 2316 96-97 -1.143589530973589 2316 98-99 -1.093308494805843 2316 100-101 -0.2692991944134633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 730.0 3 136.0 4 105.0 5 107.0 6 134.0 7 165.0 8 170.0 9 211.0 10 270.0 11 366.0 12 522.0 13 737.0 14 828.0 15 1118.0 16 1510.0 17 2071.0 18 1740.0 19 1233.0 20 1216.0 21 1265.0 22 1341.0 23 1512.0 24 1679.0 25 1775.0 26 2073.0 27 2363.0 28 2672.0 29 3121.0 30 3746.0 31 4826.0 32 6173.0 33 8322.0 34 11573.0 35 16363.0 36 23152.0 37 29267.0 38 19298.0 39 1954.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27518108250672 18.454333757896226 15.228942323774236 23.041542835822813 2 15.098392282958201 19.5524115755627 40.95948553054662 24.389710610932475 3 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21.569907060627592 26.86690477491698 29.432944451767007 22.130243712688426 48-49 21.654638098146687 26.850336746339188 29.024801890280166 22.470223265233955 50-51 21.264057406300033 27.030407078052765 29.394366106620883 22.31116940902632 52-53 21.403172602704494 27.14459326513159 28.930548678939548 22.521685453224364 54-55 21.232696153128998 27.039973252576686 28.852496961981366 22.874833632312942 56-57 21.329771674526103 26.779623968549394 29.013375507488163 22.87722884943634 58-59 21.51267257035406 26.457948281126953 29.140965268960638 22.888413879558346 60-61 20.98829322858741 26.206838914568216 30.181741036701787 22.623126820142588 62-63 20.766525658004706 26.006454682216194 30.286217581937176 22.940802077841923 64-65 20.834110121821865 25.85323519012568 30.29828677959564 23.014367908456816 66-67 20.309677336402036 25.805373226443113 30.231952632289506 23.65299680486535 68-69 20.61448060380852 25.648682704794723 30.353078831985393 23.383757859411364 70-71 20.728818081580563 25.58224759626976 30.00427544128662 23.68465888086306 72-73 20.308080028802138 25.281913799310807 30.21042277426323 24.199583397623822 74-75 20.449840559584427 25.05336110682508 30.275356169315437 24.22144216427506 76-77 20.497307723217872 24.91392750944306 30.257011974604197 24.33175279273487 78-79 19.836052398939163 24.289640761874146 30.39942136140802 25.47488547777867 80-81 20.168899261588116 24.124562401751355 30.89692773078988 24.809610605870645 82-83 20.132892715010385 24.44885205639743 31.17578227968548 24.242472948906705 84-85 19.580484763162886 24.29794195827347 30.226503514709712 25.895069763853932 86-87 19.549481209097806 24.237885235995215 30.439911541972563 25.772722012934413 88-89 19.701119275845887 23.034838344680388 30.732190270464876 26.531852109008852 90-91 19.507883190562673 21.83507176033815 32.181739284164514 26.47530576493467 92-93 19.248820254336447 21.865155392112744 32.00196731429453 26.884057039256277 94-95 17.85924366371482 22.675945420356474 32.375885954902685 27.088924961026017 96-97 17.499927672966084 22.873987019708313 33.55010013276029 26.075985174565314 98-99 18.435035858136146 25.632379366543763 30.67919906649908 25.253385708821003 100-101 18.319955510837804 25.855468084490834 28.286299331055297 27.53827707361606 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 211.0 1 123.0 2 43.5 3 49.0 4 36.5 5 31.5 6 48.0 7 87.5 8 156.0 9 210.5 10 203.5 11 196.5 12 223.5 13 229.5 14 255.5 15 291.5 16 319.5 17 332.5 18 347.5 19 383.5 20 433.5 21 509.0 22 586.5 23 688.5 24 813.0 25 941.5 26 1064.0 27 1249.0 28 1473.0 29 1644.5 30 1787.0 31 2042.0 32 2309.5 33 2513.5 34 2731.0 35 2912.0 36 3173.5 37 3480.5 38 3782.0 39 4088.0 40 4397.5 41 4618.0 42 4778.5 43 5128.0 44 5429.5 45 5564.0 46 5928.0 47 6508.5 48 6894.5 49 6946.5 50 6511.0 51 6041.0 52 5867.0 53 5775.5 54 5589.5 55 5066.5 56 4472.0 57 3893.0 58 3403.5 59 2875.0 60 2200.5 61 1701.5 62 1294.0 63 921.0 64 643.5 65 432.0 66 298.5 67 228.5 68 158.0 69 107.0 70 88.0 71 57.5 72 38.0 73 29.0 74 19.0 75 12.5 76 8.5 77 7.0 78 5.0 79 1.5 80 1.0 81 1.0 82 2.0 83 2.0 84 1.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2502502502502503 2 0.2207335540668874 3 0.22137522137522136 4 0.2181668848335515 5 0.21624188290854957 6 0.2027668694335361 7 0.20212520212520213 8 0.20661687328353992 9 0.21110854444187777 10-11 0.2210543877210544 12-13 0.21688355021688357 14-15 0.20886270886270888 16-17 0.2024460357793691 18-19 0.18961268961268962 20-21 0.20052103385436718 22-23 0.19442519442519443 24-25 0.19891686558353225 26-27 0.1925001925001925 28-29 0.19667103000436337 30-31 0.20854187520854187 32-33 0.2008418675085342 34-35 0.20116270116270116 36-37 0.19923769923769924 38-39 0.1918585251918585 40-41 0.1899335232668566 42-43 0.19827519827519827 44-45 0.19153769153769154 46-47 0.20052103385436718 48-49 0.19987936654603322 50-51 0.205975205975206 52-53 0.20854187520854187 54-55 0.20212520212520213 56-57 0.19153769153769154 58-59 0.18704602037935372 60-61 0.1880085213418547 62-63 0.19121685788352455 64-65 0.1854418521085188 66-67 0.18929185595852263 68-69 0.19121685788352455 70-71 0.19538769538769538 72-73 0.1925001925001925 74-75 0.1925001925001925 76-77 0.19667103000436337 78-79 0.19667103000436337 80-81 0.1973126973126973 82-83 0.1957085290418624 84-85 0.18191268191268192 86-87 0.18608351941685275 88-89 0.19057519057519057 90-91 0.18704602037935372 92-93 0.19378352711686045 94-95 0.18704602037935372 96-97 0.19282102615435948 98-99 0.19282102615435948 100-101 0.19282102615435948 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 155844.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.63273562487008 #Duplication Level Percentage of deduplicated Percentage of total 1 86.89500818931442 66.59002189692652 2 8.862258829809422 13.58278275887912 3 2.124336379398327 4.883811244221771 4 0.7932517469897369 2.4315620564412335 5 0.4262603768437041 1.6332749383010534 6 0.2379301255153639 1.0939941843486622 7 0.14433770089500497 0.7742695011371943 8 0.09954122571166255 0.6102493146989885 9 0.056738244322109506 0.3913206189259954 >10 0.3284841723331798 4.396058339218749 >50 0.021898943596097384 1.114461395612739 >100 0.007963252216762686 1.333387077023318 >500 0.0019908130541906716 1.1648066742646492 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 866 0.5556838890172224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 661 0.4241420908087575 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 426 0.27335027335027334 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 229 0.14694181360848027 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 228 0.1463001463001463 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 208 0.13346680013346682 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 181 0.11614178280844947 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 179 0.11485844819178154 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 164 0.10523343856677189 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.41667308333975E-4 0.0 24-25 0.0 0.0 0.0 6.41667308333975E-4 0.0 26-27 0.0 0.0 0.0 0.004170837504170837 0.0 28-29 0.0 0.0 0.0 0.010908344241677576 0.0 30-31 0.0 0.0 0.0 0.028875028875028873 0.0 32-33 0.0 0.0 0.0 0.04972921639588307 0.0 34-35 0.0 0.0 0.0 0.06545006545006544 0.0 36-37 0.0 0.0 0.0 0.09400426067092735 0.0 38-39 0.0 0.0 0.0 0.12448345781679115 0.0 40-41 0.0 0.0 0.0 0.15367932034598702 0.0 42-43 0.0 0.0 0.0 0.23035856369189706 0.0 44-45 0.0 0.0 0.0 0.31987115320448656 0.0 46-47 0.0 0.0 0.0 0.4029670696337363 0.0 48-49 0.0 0.0 0.0 0.5187880187880187 0.0 50-51 0.0 0.0 0.0 0.6599548266214933 0.0 52-53 0.0 0.0 0.0 0.949025949025949 0.0 54-55 0.0 0.0 0.0 1.184197017530351 0.0 56-57 0.0 0.0 0.0 1.670580837247504 0.0 58-59 0.0 0.0 0.0 2.32379815713149 0.0 60-61 0.0 0.0 0.0 2.965144631811299 0.0 62-63 0.0 0.0 0.0 4.001437334770668 0.0 64-65 0.0 0.0 0.0 4.622892122892123 0.0 66-67 0.0 0.0 0.0 5.7057057057057055 0.0 68-69 0.0 0.0 0.0 6.821565154898488 0.0 70-71 0.0 0.0 0.0 7.890903724237058 0.0 72-73 0.0 0.0 0.0 9.76938476938477 0.0 74-75 0.0 0.0 0.0 11.515361515361516 0.0 76-77 0.0 0.0 0.0 12.78650445317112 0.0 78-79 0.0 0.0 0.0 14.110905777572444 0.0 80-81 0.0 0.0 0.0 16.41481224814558 0.0 82-83 0.0 0.0 0.0 17.759105259105258 0.0 84-85 0.0 0.0 0.0 19.664536331202996 0.0 86-87 0.0 0.0 0.0 23.055748055748055 0.0 88-89 0.0 0.0 0.0 27.389248222581557 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACA 40 5.362876E-4 47.519466 1 GTATATA 60 1.4797188E-6 47.519463 1 AGGTAAG 50 0.0016108229 38.003345 2 AAGGTAA 70 2.0402834E-4 33.942474 1 TAAGGTG 105 1.886423E-6 31.669455 5 GTATCAA 925 0.0 29.795988 1 AATTGAA 80 4.473674E-4 29.690115 2 GTCATAC 65 0.005817077 29.24275 1 ACTGGAC 65 0.0058262344 29.233341 3 GGTAAGG 100 4.923021E-5 28.50251 3 CTACTCC 70 0.008366063 27.145247 4 CCCTGTA 70 0.008366063 27.145247 2 TTATACT 110 9.419406E-5 25.911371 4 GTATTTA 95 0.0012188717 25.010244 1 TTTATGG 95 0.0012211615 25.0022 3 GTACAAA 115 1.2712546E-4 24.792765 1 GTAAGGT 115 1.2740368E-4 24.78479 4 CAACGCA 1085 0.0 24.518288 5 ATCAACG 1090 0.0 24.405817 3 AACGCAG 1095 0.0 24.294376 6 >>END_MODULE