FastQCFastQC Report
Thu 26 May 2016
SRR937307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937307_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155844
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.49665049665049665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.41130874464207795No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5470.3509920176586843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.2778419445086112No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC2700.17325017325017325No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.10972510972510972No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1680.10780010780010779No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT250.00500805757.014641
ACGCAGT250.00501442756.9963468
CAGTACC554.9453683E-543.1790474
GTATTGG459.587303E-442.2330671
AGTACCA500.001612038937.9975625
CAGGGGT550.002573625434.5432364
TAGAAGG600.00394180631.6646354
TAAAAGC600.00394180631.6646353
TATACCA902.4007497E-531.6646355
CTCTACC650.005830610629.2288919
TACACTC650.005830610629.2288915
TCCTATG700.00837233327.1411152
CCCACAC908.916075E-426.3871963
GTATCAA12250.025.2105541
AGAACAG1201.7023268E-423.7484765
TCCAGAC1401.767584E-523.7484763
ACCTGTG1050.002188588322.6175947
CAACGCA13500.022.5170735
TCAACGC13500.022.5170734
ATCAACG13550.022.4339853