##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937307_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 155844 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44618977952311 33.0 31.0 34.0 31.0 34.0 2 32.646062729396064 34.0 31.0 34.0 31.0 34.0 3 32.70747029080363 34.0 31.0 34.0 31.0 34.0 4 36.27675111008444 37.0 35.0 37.0 35.0 37.0 5 36.231834398501064 37.0 35.0 37.0 35.0 37.0 6 36.287203870537205 37.0 37.0 37.0 35.0 37.0 7 36.2333615666949 37.0 37.0 37.0 35.0 37.0 8 36.21213521213521 37.0 37.0 37.0 35.0 37.0 9 37.9635276301943 39.0 38.0 39.0 35.0 39.0 10-11 37.89348322681656 39.0 38.0 39.0 35.0 39.0 12-13 37.79515092015092 39.0 38.0 39.0 35.0 39.0 14-15 39.08280395780396 40.0 38.0 41.0 36.0 41.0 16-17 39.05246913580247 40.0 38.0 41.0 36.0 41.0 18-19 39.020048895048895 40.0 38.0 41.0 36.0 41.0 20-21 38.92338813172147 40.0 38.0 41.0 35.5 41.0 22-23 38.87035432868766 40.0 38.0 41.0 35.0 41.0 24-25 38.87953017119683 40.0 38.0 41.0 35.0 41.0 26-27 38.72970085470085 40.0 38.0 41.0 35.0 41.0 28-29 38.58735017068351 40.0 38.0 41.0 35.0 41.0 30-31 38.630117296783965 40.0 38.0 41.0 35.0 41.0 32-33 38.513799055465725 40.0 38.0 41.0 35.0 41.0 34-35 38.382238007238 40.0 38.0 41.0 34.5 41.0 36-37 38.218651343651345 40.0 38.0 41.0 34.0 41.0 38-39 38.0851941685275 40.0 38.0 41.0 34.0 41.0 40-41 37.87114357947691 40.0 37.5 41.0 33.0 41.0 42-43 37.66371820538487 40.0 37.0 41.0 33.0 41.0 44-45 37.31614306614307 40.0 37.0 41.0 33.0 41.0 46-47 36.89014655681322 40.0 36.5 41.0 31.5 41.0 48-49 36.50842188342188 40.0 36.0 41.0 31.0 41.0 50-51 36.186439644772975 39.0 36.0 41.0 30.0 41.0 52-53 35.92829688663022 39.0 35.0 41.0 29.5 41.0 54-55 35.677661635994966 39.0 35.0 40.0 29.0 41.0 56-57 35.32410936577603 38.5 35.0 40.0 28.0 41.0 58-59 35.01951951951952 38.0 35.0 40.0 27.5 41.0 60-61 34.61598136598137 38.0 34.0 40.0 26.5 41.0 62-63 34.22762185262185 37.0 34.0 40.0 26.0 41.0 64-65 33.78891070557737 36.5 34.0 39.5 25.5 41.0 66-67 33.346073637740304 36.0 33.0 39.0 23.5 40.5 68-69 32.861149611149614 35.5 33.0 39.0 22.0 40.0 70-71 32.37817304483971 35.0 32.0 38.0 20.5 40.0 72-73 31.900239983573314 35.0 32.0 37.0 18.5 39.5 74-75 31.449045199045198 35.0 31.5 37.0 17.5 39.0 76-77 30.17657400990734 33.5 30.0 35.5 14.0 38.0 78-79 30.794759503092838 34.0 31.0 36.0 12.5 38.0 80-81 30.646460563127228 34.0 31.0 35.5 8.5 37.0 82-83 30.30871897538564 34.0 31.0 35.0 7.0 37.0 84-85 29.964490131156797 34.0 31.0 35.0 2.0 36.0 86-87 29.688717563717564 34.0 30.5 35.0 2.0 36.0 88-89 29.34965414132081 34.0 30.0 35.0 2.0 35.5 90-91 28.933725392058726 34.0 29.5 35.0 2.0 35.0 92-93 28.521197479530812 34.0 29.0 35.0 2.0 35.0 94-95 28.14934164934165 34.0 29.0 35.0 2.0 35.0 96-97 27.607370190703524 33.5 28.0 35.0 2.0 35.0 98-99 26.814198814198814 33.0 26.0 35.0 2.0 35.0 100-101 24.098297014963684 31.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17986341043057763 1101 2 0.04831767168126788 1101 3 0.23607368963348563 1101 4 0.1513873465685478 1101 5 0.16422607184782834 1101 6 0.2153860856916623 1101 7 0.09659541112090864 1101 8 0.20248059779629557 1101 9 0.28246419635170383 1101 10-11 0.14745382586101385 1101 12-13 0.08453808172325239 1101 14-15 0.3307534704938533 1101 16-17 0.2246638196983497 1101 18-19 -0.021978731273570418 1101 20-21 0.2937243668780809 1101 22-23 0.45573736046554103 1101 24-25 0.3804633056989033 1101 26-27 -0.04286730888432544 1101 28-29 -0.08065363151631999 1101 30-31 0.17006213543589865 1101 32-33 0.16958721774028618 1101 34-35 0.33317923980008146 1101 36-37 0.23181530613010892 1101 38-39 0.324573072777838 1101 40-41 0.15916184501659814 1101 42-43 0.1085985851770701 1101 44-45 0.21999643943915004 1101 46-47 0.7458772270680853 1101 48-49 0.38543504279247287 1101 50-51 0.7102493780667558 1101 52-53 0.7064534960800231 1101 54-55 0.6125348166403484 1101 56-57 0.6484092830361874 1101 58-59 1.0456468690695147 1101 60-61 1.0246624743534198 1101 62-63 1.240019468443549 1101 64-65 1.185560195685376 1101 66-67 1.1349554704222982 1101 68-69 1.406557683219681 1101 70-71 1.0051851512922383 1101 72-73 1.4268524185507339 1101 74-75 1.4009091477792204 1101 76-77 0.8204558418664831 1101 78-79 0.9682273263129169 1101 80-81 1.0749482008180138 1101 82-83 1.0823487331300115 1101 84-85 0.9675260187874493 1101 86-87 1.1138808957258135 1101 88-89 0.9290749134268275 1101 90-91 0.5451606785067966 1101 92-93 0.6355239267293058 1101 94-95 0.4534417825453154 1101 96-97 0.5306968741666118 1101 98-99 0.622791549990044 1101 100-101 1.4442351080930003 1102 1 0.09845439363309794 1102 2 0.46745845960831645 1102 3 0.17278926996185362 1102 4 0.13739576426846867 1102 5 0.27524870863545203 1102 6 0.2678260811415498 1102 7 0.2305581760699127 1102 8 0.1888302736010914 1102 9 0.15642620294935483 1102 10-11 0.2878436184519231 1102 12-13 0.3734130675041598 1102 14-15 0.38243511459956636 1102 16-17 0.47287221464772955 1102 18-19 0.655304854211586 1102 20-21 0.3787165937768009 1102 22-23 0.46235397594349337 1102 24-25 0.46424217044692995 1102 26-27 0.5664869549461073 1102 28-29 0.43572675751791934 1102 30-31 0.2609126264683894 1102 32-33 0.35858733149299127 1102 34-35 0.380879539348868 1102 36-37 0.4231852678333681 1102 38-39 0.5680228130425036 1102 40-41 0.5125156538362319 1102 42-43 0.26474312694326585 1102 44-45 0.2796218138751243 1102 46-47 0.5204762487948571 1102 48-49 0.2932953544748713 1102 50-51 -0.05375168362512994 1102 52-53 -0.09650786591899418 1102 54-55 -0.01763619158775498 1102 56-57 -0.0832480938265121 1102 58-59 0.10703541038070341 1102 60-61 0.2612870525204869 1102 62-63 -0.43828789135624646 1102 64-65 0.005811968331634887 1102 66-67 0.36619575404570526 1102 68-69 0.4647990664525352 1102 70-71 0.43845023300460184 1102 72-73 0.7857421921828625 1102 74-75 1.1868455221014464 1102 76-77 0.9594995608214738 1102 78-79 0.19690817414972273 1102 80-81 0.6100409092698413 1102 82-83 0.700158614448025 1102 84-85 1.037844147176333 1102 86-87 1.0580472780107257 1102 88-89 0.9391230687371817 1102 90-91 1.3467190905190805 1102 92-93 1.3502511098332377 1102 94-95 1.4759837762509989 1102 96-97 1.9872433421452271 1102 98-99 1.306236740146641 1102 100-101 1.8772916431857105 1103 1 0.20250897236736165 1103 2 0.3037222568239102 1103 3 0.3529434659793793 1103 4 0.1747799785898394 1103 5 0.1649264500520573 1103 6 0.20403995878312742 1103 7 0.0463549479374592 1103 8 0.1853913696130718 1103 9 0.26957723739386097 1103 10-11 -0.04398288329526423 1103 12-13 0.2835855673654919 1103 14-15 0.3299363625889171 1103 16-17 0.29049937089599354 1103 18-19 0.2080254709956577 1103 20-21 0.3956060496534377 1103 22-23 0.3191402647004864 1103 24-25 0.19010050978911153 1103 26-27 0.0611622003840111 1103 28-29 0.30679559106386733 1103 30-31 0.009512105086173506 1103 32-33 0.1102651838419817 1103 34-35 -0.15264977453431783 1103 36-37 0.12047851759763972 1103 38-39 0.31257325954535986 1103 40-41 -0.1854709233449796 1103 42-43 0.24935125828721283 1103 44-45 -0.414149332611089 1103 46-47 -0.29271361198500045 1103 48-49 -0.5191734457913597 1103 50-51 -0.46059621188515365 1103 52-53 -0.15125299876052622 1103 54-55 -0.24110948461566295 1103 56-57 -0.4717055789893081 1103 58-59 -0.49737969861035936 1103 60-61 -0.39257616784669835 1103 62-63 -0.22391105403858802 1103 64-65 -0.47613378573125686 1103 66-67 -0.10546102114981437 1103 68-69 -0.10702965394238362 1103 70-71 0.00611898889788165 1103 72-73 -0.019848800107336473 1103 74-75 -0.047776475790843875 1103 76-77 -0.2875191312856842 1103 78-79 -0.0016330649650377893 1103 80-81 -0.03284000481302485 1103 82-83 -0.14403684841736109 1103 84-85 0.26880537630717427 1103 86-87 0.21604272978287042 1103 88-89 0.4331604529515012 1103 90-91 0.7341227180081269 1103 92-93 0.304852030572043 1103 94-95 -0.13980190237787937 1103 96-97 0.6096294977833665 1103 98-99 0.5580532579790223 1103 100-101 2.1719280622882664 1104 1 0.20939602470895835 1104 2 0.2940803835456762 1104 3 0.015477901241091274 1104 4 0.18579926233639554 1104 5 0.018232235176022016 1104 6 0.06836502765365537 1104 7 0.18891693140853505 1104 8 0.03594233380039924 1104 9 0.3005689729310532 1104 10-11 0.14334494039619727 1104 12-13 -0.3569103004031007 1104 14-15 0.03895840115640681 1104 16-17 0.18684923315495183 1104 18-19 0.04273621479730849 1104 20-21 -0.14123968037411316 1104 22-23 -0.24559802731052827 1104 24-25 -0.07023006872329063 1104 26-27 0.06081784776693411 1104 28-29 0.06919228527874566 1104 30-31 0.11247353759306122 1104 32-33 0.05344700202379471 1104 34-35 -0.09652029795029904 1104 36-37 -0.23730385154836853 1104 38-39 -0.11270222254830742 1104 40-41 0.19710483423077818 1104 42-43 0.30961296627619816 1104 44-45 0.9016220172511709 1104 46-47 0.7234709610177603 1104 48-49 0.4184987305171788 1104 50-51 0.41543684596608443 1104 52-53 0.12870567892542084 1104 54-55 0.4296894134559608 1104 56-57 0.8302916661897584 1104 58-59 0.9213530837587882 1104 60-61 0.9397241076353922 1104 62-63 0.7781550616638881 1104 64-65 0.9391554704670924 1104 66-67 1.0126519265085818 1104 68-69 0.4948987207132589 1104 70-71 0.7158297326995324 1104 72-73 0.3758123569174572 1104 74-75 0.888518290049376 1104 76-77 1.180111722708805 1104 78-79 0.7924440700211868 1104 80-81 0.8865814827902803 1104 82-83 0.44515047940633323 1104 84-85 0.8173590953154424 1104 86-87 0.9932465865321802 1104 88-89 1.1418381389019174 1104 90-91 0.821932635363499 1104 92-93 0.4966564382855445 1104 94-95 1.2203909350876891 1104 96-97 1.494615723678681 1104 98-99 1.8614279136264038 1104 100-101 2.029366078817194 1105 1 -0.01250035847775166 1105 2 0.27526317924459676 1105 3 0.10394319938380647 1105 4 0.09220199938429374 1105 5 0.11720584221414754 1105 6 -0.07288619424272724 1105 7 -0.0552179659521741 1105 8 0.18525807182581389 1105 9 0.22505577645011954 1105 10-11 0.11120906551838772 1105 12-13 0.1504210778960271 1105 14-15 0.20147062012024008 1105 16-17 -0.22346357231914027 1105 18-19 0.28980366347766306 1105 20-21 0.06379453272462143 1105 22-23 0.3087763617412804 1105 24-25 -0.1886318282540813 1105 26-27 -0.2860785354197688 1105 28-29 -0.159180148738848 1105 30-31 0.27877363534476984 1105 32-33 -0.1518315903515699 1105 34-35 -0.14185265376652723 1105 36-37 -0.2099333725649828 1105 38-39 0.15061644802842977 1105 40-41 0.17340004439695633 1105 42-43 0.05533632893504148 1105 44-45 0.19475495957765787 1105 46-47 0.07378865407738289 1105 48-49 0.8336991215240133 1105 50-51 0.4843518019778159 1105 52-53 0.8053918959342283 1105 54-55 1.0621429813934071 1105 56-57 0.4502818910186974 1105 58-59 -0.24934584097239565 1105 60-61 -0.13774412305864558 1105 62-63 0.237832481018728 1105 64-65 0.2019853824905482 1105 66-67 -0.010319725495328669 1105 68-69 -0.34576599091919746 1105 70-71 -0.3505192409075022 1105 72-73 -0.521548346894857 1105 74-75 -0.9519020423064397 1105 76-77 -0.5703770358444693 1105 78-79 -0.3828980609661059 1105 80-81 -0.5749287811393415 1105 82-83 -0.2156510846210402 1105 84-85 0.14690454986089563 1105 86-87 0.4409494213317906 1105 88-89 0.37387626206907143 1105 90-91 -0.053800502466412325 1105 92-93 0.27109436594928127 1105 94-95 0.2217350211091933 1105 96-97 0.8782911879993094 1105 98-99 0.6322001521405802 1105 100-101 1.602313292893438 1106 1 0.037025258220545254 1106 2 0.0725117560946913 1106 3 0.11191283885249703 1106 4 0.20861566162338363 1106 5 0.08267073784982415 1106 6 0.21658249645935967 1106 7 0.29506666402885173 1106 8 0.24654839440646015 1106 9 0.08425881250324352 1106 10-11 -0.10781370488009401 1106 12-13 -0.3970172522747504 1106 14-15 0.04943077192289991 1106 16-17 0.20552124741519862 1106 18-19 -0.23533865151284772 1106 20-21 0.057200604830882185 1106 22-23 0.20056953062173477 1106 24-25 0.1672030863569276 1106 26-27 0.16344708127851248 1106 28-29 0.18175913019853596 1106 30-31 0.49585143420625144 1106 32-33 0.29261812501845696 1106 34-35 -0.21225113573996168 1106 36-37 -0.08360509932020221 1106 38-39 -0.3463474988406432 1106 40-41 -0.2325297468743912 1106 42-43 -0.3365991549359322 1106 44-45 -0.39708564763296295 1106 46-47 -0.6204238696417832 1106 48-49 -1.3259308595006516 1106 50-51 -0.9733332075098602 1106 52-53 -1.8805189200050592 1106 54-55 -1.502752654280222 1106 56-57 -1.6413215245410768 1106 58-59 -1.621784171143176 1106 60-61 -1.9418712577834967 1106 62-63 -1.9541978795125843 1106 64-65 -1.688384636484784 1106 66-67 -1.623934526254338 1106 68-69 -1.3806806019248867 1106 70-71 -1.6388494116854915 1106 72-73 -1.2665673222269263 1106 74-75 -0.9673194996119427 1106 76-77 -1.1417242844213185 1106 78-79 -1.9919587820287212 1106 80-81 -1.237215308653571 1106 82-83 -1.037425278169426 1106 84-85 -0.7501542736685174 1106 86-87 -1.0623238234499937 1106 88-89 -1.2525468878360506 1106 90-91 -1.2843507870963933 1106 92-93 -1.039484381678804 1106 94-95 -1.080589457069177 1106 96-97 -0.5935304438792492 1106 98-99 0.24239939312729675 1106 100-101 0.23177249592949067 1107 1 0.046749147776367295 1107 2 0.23625725429397448 1107 3 0.07793924941672259 1107 4 0.19012826666540406 1107 5 -0.17147091881284382 1107 6 0.043473252761877745 1107 7 0.143926207846377 1107 8 0.06083410869217687 1107 9 0.320513314303966 1107 10-11 0.2600734499818529 1107 12-13 0.28455537677686493 1107 14-15 -0.11263405400747217 1107 16-17 -0.193098200363913 1107 18-19 -0.11589087240120932 1107 20-21 0.33379124108538605 1107 22-23 -0.22998269026661688 1107 24-25 -0.2770946327307158 1107 26-27 -0.3645841312064775 1107 28-29 -0.10659621193832436 1107 30-31 -0.3593106306196745 1107 32-33 -0.04789808146414032 1107 34-35 0.007916931486931844 1107 36-37 0.02467821400512804 1107 38-39 -0.004090596079535658 1107 40-41 -0.0630219437531423 1107 42-43 -0.05904813452776381 1107 44-45 0.2930103907823991 1107 46-47 0.539720187981267 1107 48-49 0.9165661392988724 1107 50-51 0.8727652318658912 1107 52-53 0.7676203356972238 1107 54-55 0.5450264573644432 1107 56-57 0.45513705889229783 1107 58-59 0.0965231517859948 1107 60-61 -0.37482443905600604 1107 62-63 0.22203261268429486 1107 64-65 0.11401632389937078 1107 66-67 -0.21547114090019903 1107 68-69 -0.04112477959595395 1107 70-71 0.06949392564943935 1107 72-73 -0.14111714215489712 1107 74-75 0.08850282931963349 1107 76-77 0.4529936027335424 1107 78-79 0.026828732977278946 1107 80-81 0.2836130226789635 1107 82-83 0.6033910483611855 1107 84-85 0.539761692718038 1107 86-87 0.17885264713824256 1107 88-89 0.4011145767497837 1107 90-91 1.0986023941761154 1107 92-93 1.314297130926235 1107 94-95 1.1421596425706788 1107 96-97 0.8537684756885788 1107 98-99 1.651703114615895 1107 100-101 2.736694464717008 1108 1 0.09806532642173948 1108 2 -0.032006572976065684 1108 3 0.04643969307244333 1108 4 -0.12250113292051878 1108 5 -0.11187316666850933 1108 6 0.0476140395113589 1108 7 0.18134117383277726 1108 8 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-0.37844481930503804 2316 76-77 -0.3811530994255854 2316 78-79 -0.2316007257627355 2316 80-81 -0.23627754224266084 2316 82-83 0.03721240298999717 2316 84-85 -0.2443997979651904 2316 86-87 -0.32477054126755434 2316 88-89 0.14941389647767167 2316 90-91 0.00720931520539736 2316 92-93 0.318955516019404 2316 94-95 0.7819851379862364 2316 96-97 0.707066316564056 2316 98-99 0.4923567673813487 2316 100-101 1.6242277388962414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 1.0 5 6.0 6 5.0 7 12.0 8 35.0 9 79.0 10 102.0 11 173.0 12 331.0 13 431.0 14 612.0 15 837.0 16 1241.0 17 1906.0 18 1727.0 19 1038.0 20 877.0 21 913.0 22 932.0 23 903.0 24 1009.0 25 1184.0 26 1318.0 27 1621.0 28 1790.0 29 2172.0 30 2723.0 31 3513.0 32 4774.0 33 6726.0 34 9749.0 35 15305.0 36 25110.0 37 37439.0 38 27073.0 39 2175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73964846508403 19.672089052088282 14.840730802316177 21.74753168051151 2 16.005107671774336 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20-21 22.78688945355612 26.17136367136367 29.595942095942096 21.44580477913811 22-23 22.500850214639094 26.03228890614272 29.745320611127866 21.721540268090322 24-25 22.778941694712966 26.305235701200242 29.547591012778845 21.368231591307946 26-27 22.162510748065348 26.3661272314267 29.813529087152375 21.65783293335558 28-29 21.864552518945835 27.310557690950276 29.4467366962057 21.378153093898188 30-31 22.27804727804728 26.95483945483945 29.028387361720696 21.738725905392574 32-33 22.350876517543185 26.70298503631837 28.981545648212315 21.96459279792613 34-35 22.529580862914198 26.94938528271862 29.180783347450014 21.340250506917176 36-37 22.879289545956212 26.530376530376532 29.119824953158286 21.47050897050897 38-39 22.222863889530554 27.019647852981187 29.27061677061677 21.486871486871486 40-41 22.117951284617952 27.22915222915223 28.917378917378915 21.735517568850902 42-43 22.285683115516296 27.43050764792895 28.403063663754242 21.88074557280052 44-45 22.595030928364263 27.283052283052285 28.28854495521162 21.833371833371835 46-47 22.944739611406277 27.863761197094533 27.837773671107 21.353725520392185 48-49 22.72849772849773 27.97797797797798 27.245835579168915 22.04768871435538 50-51 22.5674392341059 28.145453145453146 27.55800672467339 21.729100895767562 52-53 22.514501681168348 28.532057698724365 26.901901901901905 22.051538718205386 54-55 22.383280716614053 28.569274402607736 26.875914375914377 22.17153050486384 56-57 22.717268550601887 28.158607325273994 27.068414568414568 22.055709555709555 58-59 22.52669336002669 27.70815687482354 27.374810708144043 22.390339057005722 60-61 22.089076255742924 28.046315546315547 27.83520700187367 22.029401196067862 62-63 21.65370498703832 27.90739457406124 28.162778162778164 22.276122276122276 64-65 21.754767588100922 28.235286568619898 28.065886399219732 21.944059444059445 66-67 21.32035882035882 27.603565103565103 28.054015554015553 23.02206052206052 68-69 21.641834141834142 27.31770231770232 28.11946561946562 22.92099792099792 70-71 21.816046816046818 27.749865249865252 27.760452760452758 22.673635173635173 72-73 21.209029542362874 27.029272862606195 27.889427889427886 23.87226970560304 74-75 21.294371294371295 27.164343831010495 27.80633197299864 23.734952901619568 76-77 21.15320448653782 26.652614152614152 28.049203049203047 24.14497831164498 78-79 20.70788737455404 26.27563460896794 27.9632196298863 25.05325838659172 80-81 20.95621262287929 26.008059341392674 28.48008264674931 24.55564538897872 82-83 20.912325506036506 26.59174107357702 28.3826402769445 24.11329314344198 84-85 19.80698647365314 27.36005236005236 27.713290213290215 25.119670953004285 86-87 19.855432355432356 26.357126357126358 27.516298349631686 26.271142937809604 88-89 19.765919765919765 25.9679551346218 28.08962808962809 26.176497009830342 90-91 19.827884948596513 24.322641921117143 28.835046782950215 27.01442634733613 92-93 19.8139782600326 24.489867943173213 28.700222019737943 26.99593177705625 94-95 18.432855932855933 25.96121762788429 28.750866250866252 26.85506018839352 96-97 18.044005544005547 26.893881060547724 29.49038782372116 25.571725571725572 98-99 17.97969103421724 29.23592729839421 26.506889969039253 26.27749169834929 100-101 18.048152795114767 30.386383983724446 24.378034418691215 27.187428802469572 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34.0 1 26.5 2 16.0 3 22.0 4 32.0 5 38.5 6 43.0 7 77.5 8 125.0 9 140.0 10 154.5 11 175.5 12 204.0 13 218.0 14 231.0 15 273.0 16 303.5 17 332.0 18 352.5 19 379.5 20 434.0 21 485.5 22 540.0 23 628.0 24 722.5 25 806.0 26 968.0 27 1177.0 28 1343.0 29 1525.0 30 1699.5 31 1933.0 32 2228.0 33 2321.5 34 2434.5 35 2735.5 36 3068.5 37 3412.5 38 3669.0 39 3901.5 40 4144.0 41 4475.0 42 4652.5 43 4940.5 44 5342.0 45 5514.0 46 5867.0 47 6445.0 48 6984.0 49 6950.0 50 6561.0 51 6251.5 52 6089.0 53 6108.5 54 6009.0 55 5455.0 56 4741.0 57 4205.0 58 3743.5 59 3145.5 60 2384.5 61 1818.0 62 1437.0 63 1024.5 64 668.5 65 449.0 66 331.5 67 268.5 68 208.5 69 163.5 70 108.0 71 55.5 72 34.0 73 25.5 74 19.0 75 12.5 76 9.0 77 4.0 78 2.0 79 2.0 80 1.0 81 0.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04491671158337825 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0038500038500038497 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.41667308333975E-4 24-25 3.208336541669875E-4 26-27 0.00128333461666795 28-29 0.0032083365416698748 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011229177895844562 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.208336541669875E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.011550011550011549 92-93 0.00128333461666795 94-95 0.0 96-97 0.0 98-99 9.625009625009624E-4 100-101 0.01764585097918431 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 155844.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.8586340978222 #Duplication Level Percentage of deduplicated Percentage of total 1 85.73283092841832 63.321097897125036 2 9.13393746550906 13.492402902748463 3 2.4330840872412005 5.391128019863445 4 1.0417356912938511 3.0776470099965767 5 0.5173380795506891 1.910494196120219 6 0.30097404016684565 1.3337718903375775 7 0.18934752549124217 0.9789464721810004 8 0.131545782424045 0.7772633448935417 9 0.07872805365972795 0.5233271857638803 >10 0.4046022822035584 5.172178657951383 >50 0.020927704024187015 1.0473900929641042 >100 0.011958688013821151 1.5302347176896507 >500 0.0029896720034552878 1.444117612365125 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 774 0.49665049665049665 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 641 0.41130874464207795 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 547 0.3509920176586843 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 433 0.2778419445086112 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 270 0.17325017325017325 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 171 0.10972510972510972 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 168 0.10780010780010779 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.41667308333975E-4 0.0 24-25 0.0 0.0 0.0 6.41667308333975E-4 0.0 26-27 0.0 0.0 0.0 0.004170837504170837 0.0 28-29 0.0 0.0 0.0 0.010908344241677576 0.0 30-31 0.0 0.0 0.0 0.028233361566694898 0.0 32-33 0.0 0.0 0.0 0.04844588177921512 0.0 34-35 0.0 0.0 0.0 0.0641667308333975 0.0 36-37 0.0 0.0 0.0 0.09304175970842637 0.0 38-39 0.0 0.0 0.0 0.12255845589178924 0.0 40-41 0.0 0.0 0.0 0.15207515207515207 0.0 42-43 0.0 0.0 0.0 0.22971689638356307 0.0 44-45 0.0 0.0 0.0 0.32243782243782243 0.0 46-47 0.0 0.0 0.0 0.4081004081004081 0.0 48-49 0.0 0.0 0.0 0.5271296937963605 0.0 50-51 0.0 0.0 0.0 0.6663714997048331 0.0 52-53 0.0 0.0 0.0 0.9560842894176227 0.0 54-55 0.0 0.0 0.0 1.1947845281178615 0.0 56-57 0.0 0.0 0.0 1.6824516824516824 0.0 58-59 0.0 0.0 0.0 2.33663150329817 0.0 60-61 0.0 0.0 0.0 2.9863196529863196 0.0 62-63 0.0 0.0 0.0 4.032879032879033 0.0 64-65 0.0 0.0 0.0 4.656900490233824 0.0 66-67 0.0 0.0 0.0 5.743884910551577 0.0 68-69 0.0 0.0 0.0 6.867123533790201 0.0 70-71 0.0 0.0 0.0 7.944162110828778 0.0 72-73 0.0 0.0 0.0 9.844459844459845 0.0 74-75 0.0 0.0 0.0 11.607761607761606 0.0 76-77 0.0 0.0 0.0 12.894304560971229 0.0 78-79 0.0 0.0 0.0 14.243730910397577 0.0 80-81 0.0 0.0 0.0 16.59383742717076 0.0 82-83 0.0 0.0 0.0 17.95481378814712 0.0 84-85 0.0 0.0 0.0 19.88879905546572 0.0 86-87 0.0 0.0 0.0 23.350915017581684 0.0 88-89 0.0 0.0 0.0 27.79574446241113 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGT 25 0.005008057 57.01464 1 ACGCAGT 25 0.005014427 56.996346 8 CAGTACC 55 4.9453683E-5 43.179047 4 GTATTGG 45 9.587303E-4 42.233067 1 AGTACCA 50 0.0016120389 37.997562 5 CAGGGGT 55 0.0025736254 34.543236 4 TAGAAGG 60 0.003941806 31.664635 4 TAAAAGC 60 0.003941806 31.664635 3 TATACCA 90 2.4007497E-5 31.664635 5 CTCTACC 65 0.0058306106 29.228891 9 TACACTC 65 0.0058306106 29.228891 5 TCCTATG 70 0.008372333 27.141115 2 CCCACAC 90 8.916075E-4 26.387196 3 GTATCAA 1225 0.0 25.210554 1 AGAACAG 120 1.7023268E-4 23.748476 5 TCCAGAC 140 1.767584E-5 23.748476 3 ACCTGTG 105 0.0021885883 22.617594 7 CAACGCA 1350 0.0 22.517073 5 TCAACGC 1350 0.0 22.517073 4 ATCAACG 1355 0.0 22.433985 3 >>END_MODULE