FastQCFastQC Report
Thu 26 May 2016
SRR937306_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937306_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140895
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.5926399091522055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.46488519819723906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.28389935767770325No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2640.18737357606728414No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2100.14904716278079422No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1750.12420596898399516No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1640.11639873664785834No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1640.11639873664785834No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.10646225912913873No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCC250.005005460757.0192037
CTAAGGC608.237289E-539.596673
GGTATCA5400.036.0897981
GTATCAA8300.035.5065081
CTTATAT702.040171E-433.9400022
TATATCT600.003925933531.6886064
GACAGGT650.00581728929.2406167
TTATATC650.00581728929.2406163
TCAACGC10150.029.0348784
TATCAAC10000.028.984762
CAACGCA10200.028.892555
ATCAACG10250.028.7413883
AACGCAG10400.028.3268456
CAATGCA856.3664804E-427.950599
AATACAT700.00833875627.1616615
ATACCAT700.00835324627.1526
CTGTGCA1257.311577E-626.6089619
CCACACT908.872426E-426.407174
ACGCAGA11300.026.0707267
CTTGGAC1109.3972136E-525.917823