##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937306_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 140895 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9943645977501 33.0 31.0 34.0 31.0 34.0 2 32.11179246956954 33.0 31.0 34.0 31.0 34.0 3 32.148500656517264 34.0 31.0 34.0 30.0 34.0 4 35.75365342985911 37.0 35.0 37.0 35.0 37.0 5 35.73611554703857 37.0 35.0 37.0 35.0 37.0 6 35.789900280350615 37.0 35.0 37.0 35.0 37.0 7 35.73295006920047 37.0 35.0 37.0 35.0 37.0 8 35.736626565882396 37.0 35.0 37.0 35.0 37.0 9 37.40917704673693 39.0 37.0 39.0 35.0 39.0 10-11 37.3007097483942 39.0 37.0 39.0 35.0 39.0 12-13 37.18398452748501 39.0 37.0 39.0 34.0 39.0 14-15 38.41634195677632 40.0 38.0 41.0 34.0 41.0 16-17 38.390404201710496 40.0 38.0 41.0 34.0 41.0 18-19 38.38106036410093 40.0 38.0 41.0 34.0 41.0 20-21 38.296862912097666 40.0 38.0 41.0 34.0 41.0 22-23 38.171663295361796 40.0 38.0 41.0 34.0 41.0 24-25 38.03227225948402 40.0 38.0 41.0 34.0 41.0 26-27 37.867394158770715 40.0 38.0 41.0 33.0 41.0 28-29 37.8874942332943 40.0 38.0 41.0 33.0 41.0 30-31 37.68893502253451 40.0 37.5 41.0 33.0 41.0 32-33 37.46439902054722 40.0 37.0 41.0 33.0 41.0 34-35 37.3289612832251 40.0 37.0 41.0 33.0 41.0 36-37 37.353188544660924 40.0 37.0 41.0 33.0 41.0 38-39 37.234039532985555 40.0 37.0 41.0 32.5 41.0 40-41 37.03065758188722 40.0 36.5 41.0 31.5 41.0 42-43 36.74044856098513 40.0 36.0 41.0 31.0 41.0 44-45 36.44286170552539 40.0 36.0 41.0 30.5 41.0 46-47 35.98626281983037 39.0 35.5 41.0 29.5 41.0 48-49 35.568320380425135 39.0 35.0 41.0 27.5 41.0 50-51 34.648213208417616 38.0 34.0 40.0 25.0 40.5 52-53 34.677628730615 38.5 35.0 40.0 26.0 40.5 54-55 34.93862805635402 39.0 35.0 40.0 25.5 41.0 56-57 34.700251960679935 39.0 35.0 40.0 24.0 41.0 58-59 34.4220660775755 38.0 34.5 40.0 23.0 41.0 60-61 34.08186947727031 38.0 34.0 40.0 22.0 41.0 62-63 33.72897547819298 37.5 34.0 40.0 19.5 41.0 64-65 33.31113595230491 37.0 33.5 40.0 18.0 41.0 66-67 32.87598211434047 36.5 33.0 39.0 12.0 41.0 68-69 32.39141914191419 36.0 32.5 39.0 7.5 41.0 70-71 31.90653323396856 35.0 32.0 39.0 2.0 40.0 72-73 31.52013201320132 35.0 32.0 38.0 2.0 40.0 74-75 31.03837609567408 35.0 31.0 37.0 2.0 39.5 76-77 30.620000709748396 35.0 31.0 37.0 2.0 39.0 78-79 30.19437169523404 35.0 31.0 36.0 2.0 39.0 80-81 29.751918095035307 34.5 30.0 36.0 2.0 37.5 82-83 29.296153163703465 34.0 29.5 35.0 2.0 37.0 84-85 28.91126015827389 34.0 29.0 35.0 2.0 37.0 86-87 28.518943184641046 34.0 29.0 35.0 2.0 36.0 88-89 28.168973348947798 34.0 29.0 35.0 2.0 36.0 90-91 27.75810000354874 34.0 27.5 35.0 2.0 35.5 92-93 27.342155505873166 34.0 26.5 35.0 2.0 35.0 94-95 26.879012739983676 34.0 25.5 35.0 2.0 35.0 96-97 26.33128216047411 33.0 25.0 35.0 2.0 35.0 98-99 25.58673480251251 33.0 22.0 35.0 2.0 35.0 100-101 23.727815039568473 30.5 8.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.09139404114085536 1101 2 -0.16612617706493182 1101 3 -0.27356175561146756 1101 4 0.021161295209232378 1101 5 -0.27131122916978256 1101 6 -0.5924788365464124 1101 7 -0.3078668213075204 1101 8 -0.09761736226730733 1101 9 -0.1956157477143492 1101 10-11 -0.3597444093961286 1101 12-13 -0.46679913292203423 1101 14-15 -0.38923238045404673 1101 16-17 -0.31377875045681236 1101 18-19 -0.01760002032749952 1101 20-21 -0.3863114255195583 1101 22-23 -0.6724788986297696 1101 24-25 -0.8159370418203125 1101 26-27 -0.47390962699574857 1101 28-29 -0.578796589709448 1101 30-31 -0.570051937869799 1101 32-33 -0.46164236989864804 1101 34-35 -0.20635643980234875 1101 36-37 -0.03267082061816495 1101 38-39 -0.25079837589760956 1101 40-41 -0.1263593760745394 1101 42-43 0.16899011953621113 1101 44-45 0.4642350377894573 1101 46-47 0.5107909034156464 1101 48-49 0.4044998492843206 1101 50-51 0.48303711724704357 1101 52-53 0.9060416499104988 1101 54-55 1.1393333375777601 1101 56-57 0.7378482648265745 1101 58-59 1.1508756059418346 1101 60-61 0.8410261266825216 1101 62-63 0.7492541772026726 1101 64-65 0.8340525463842496 1101 66-67 0.6212633072914429 1101 68-69 0.9032104014392957 1101 70-71 0.7286793979929946 1101 72-73 0.8029737776488943 1101 74-75 1.0332613769101542 1101 76-77 0.8438798752869943 1101 78-79 0.3680783270236354 1101 80-81 0.37620672985896775 1101 82-83 0.3138850832538296 1101 84-85 0.4836140428249749 1101 86-87 0.583644776833939 1101 88-89 0.6247068136930736 1101 90-91 0.6271064797657289 1101 92-93 0.32521026939166475 1101 94-95 0.08443573894048129 1101 96-97 -0.07719051899905338 1101 98-99 0.8508213858520044 1101 100-101 1.476002898986561 1102 1 0.06191874776515505 1102 2 0.1594563041415853 1102 3 0.09140532661292866 1102 4 0.1056735985196866 1102 5 -0.011377531639780614 1102 6 -0.30495023776973795 1102 7 0.15571351207250217 1102 8 -0.007782184604700149 1102 9 -0.3652940406592009 1102 10-11 0.07642779025835011 1102 12-13 0.1738368104874084 1102 14-15 0.12266237604779917 1102 16-17 0.48510764819846486 1102 18-19 0.2782630790795153 1102 20-21 0.2911994303426084 1102 22-23 0.29623754158128435 1102 24-25 0.15149537168297655 1102 26-27 0.4209052396989392 1102 28-29 0.3584028313112455 1102 30-31 0.397392148603565 1102 32-33 0.6056523025578073 1102 34-35 0.5210213442010172 1102 36-37 -0.13285992273330294 1102 38-39 -0.0657818002801136 1102 40-41 0.8470962899851386 1102 42-43 0.6873517014571107 1102 44-45 0.14509603606186516 1102 46-47 0.5318956333031153 1102 48-49 0.8757259780605224 1102 50-51 0.6812091301340004 1102 52-53 1.164679647762668 1102 54-55 0.7955176771211114 1102 56-57 0.716965321370445 1102 58-59 0.8341762549589689 1102 60-61 0.8542982936526897 1102 62-63 0.8129068829971331 1102 64-65 0.9222768541771202 1102 66-67 0.6172478055871835 1102 68-69 0.5799625142468727 1102 70-71 1.3354170363176898 1102 72-73 0.9659401128032137 1102 74-75 0.7844987117376299 1102 76-77 1.3171487738255365 1102 78-79 0.9056769636207314 1102 80-81 1.151046810169003 1102 82-83 0.9781354451159032 1102 84-85 0.7841463302601923 1102 86-87 0.9981636170076307 1102 88-89 0.6323059172089707 1102 90-91 0.7106732725004719 1102 92-93 0.4015048017724858 1102 94-95 0.6424854191011029 1102 96-97 0.9295681306717718 1102 98-99 1.0021380968759566 1102 100-101 1.5081852774709432 1103 1 -0.34110635414430845 1103 2 -0.47588661225689677 1103 3 -0.20488302352652354 1103 4 -0.3676869106700451 1103 5 -0.1859466512850645 1103 6 -0.01234493132620429 1103 7 0.07611685899376397 1103 8 0.15015960185027666 1103 9 -0.030744945329161055 1103 10-11 -0.17021098539780155 1103 12-13 -0.3486905441575203 1103 14-15 -0.4558973035631553 1103 16-17 -0.37693912288070663 1103 18-19 -0.2821674436981496 1103 20-21 -0.4907679586297178 1103 22-23 -0.9237081067059236 1103 24-25 -0.5950718885616126 1103 26-27 -0.28529157071371003 1103 28-29 -0.43212852819673486 1103 30-31 -0.44809880469687613 1103 32-33 -0.8951343669694722 1103 34-35 -0.6287855641877442 1103 36-37 -0.23219482940568525 1103 38-39 -0.08543065960929397 1103 40-41 0.2615996154517788 1103 42-43 -0.06518709565366265 1103 44-45 -0.11024974095308693 1103 46-47 -0.37277432307248404 1103 48-49 -0.04635798103266353 1103 50-51 -0.10075825883832579 1103 52-53 0.05608349529180856 1103 54-55 -0.07248275197135712 1103 56-57 -0.45411742196073845 1103 58-59 0.09365204699215468 1103 60-61 0.31476814990671187 1103 62-63 -0.04837109740764589 1103 64-65 0.04814397854242003 1103 66-67 0.19446657123118882 1103 68-69 0.6963896042847821 1103 70-71 1.0081803918207974 1103 72-73 0.7973246511712304 1103 74-75 1.3840302857918338 1103 76-77 1.4125324540086197 1103 78-79 0.9686641623620602 1103 80-81 0.5243393797177411 1103 82-83 0.516521342276743 1103 84-85 0.5814537750145377 1103 86-87 0.5714311561623191 1103 88-89 0.33741211759515366 1103 90-91 0.6140559514677904 1103 92-93 0.535614077321366 1103 94-95 0.9133142827212133 1103 96-97 0.9407245348768782 1103 98-99 0.8979758999225105 1103 100-101 1.316702762989074 1104 1 0.18421010155189776 1104 2 0.03677161559120634 1104 3 0.04195241951145334 1104 4 -0.04490210054346022 1104 5 0.12417993099341373 1104 6 0.08892089145860638 1104 7 0.22801559317097997 1104 8 0.23243808286294154 1104 9 0.1527993584683074 1104 10-11 0.5038396475135869 1104 12-13 0.2747271773614699 1104 14-15 0.5567609242849372 1104 16-17 0.37306087711929337 1104 18-19 0.18618698668159794 1104 20-21 0.3320168514968671 1104 22-23 0.4148994882307804 1104 24-25 0.265687605109278 1104 26-27 0.22420210017236286 1104 28-29 0.3843271680057967 1104 30-31 0.6594961320119879 1104 32-33 0.31056183556217576 1104 34-35 0.37121443581225577 1104 36-37 0.17919757565759653 1104 38-39 0.18355668216285181 1104 40-41 0.11919455216062858 1104 42-43 -0.0873389943878422 1104 44-45 0.32962367676843485 1104 46-47 0.9753269427503 1104 48-49 1.1530091075749311 1104 50-51 1.1935455386300262 1104 52-53 0.891526533266493 1104 54-55 1.041441298661553 1104 56-57 1.014237008419002 1104 58-59 1.001879895093417 1104 60-61 1.2578061245902603 1104 62-63 1.2459327000607132 1104 64-65 0.8867515734791311 1104 66-67 1.460289356041315 1104 68-69 1.3008199840316195 1104 70-71 1.3436234297954819 1104 72-73 1.5188436385130082 1104 74-75 1.15934674148804 1104 76-77 1.0581020742617824 1104 78-79 1.35474011172915 1104 80-81 1.1952254556671065 1104 82-83 0.896268177719783 1104 84-85 0.980187952229727 1104 86-87 1.0207982447699138 1104 88-89 0.919690598607815 1104 90-91 1.1900053185563984 1104 92-93 1.3805507861821233 1104 94-95 0.8278712447465288 1104 96-97 1.0261675728515627 1104 98-99 1.4770898239731451 1104 100-101 1.9812597250143895 1105 1 0.2046334607690028 1105 2 0.07618231658046426 1105 3 0.013923486419230358 1105 4 0.2189528085087673 1105 5 0.013500216069473936 1105 6 -0.051330145665104965 1105 7 0.4677773206467819 1105 8 0.46783857217258884 1105 9 0.6147183032614194 1105 10-11 0.33242171325127856 1105 12-13 0.2550218268668374 1105 14-15 -0.20746628558634228 1105 16-17 -0.03006113117768905 1105 18-19 0.08087904071840768 1105 20-21 -0.20103814265269193 1105 22-23 -0.004550570273629262 1105 24-25 0.11631519791855283 1105 26-27 0.49273736237852006 1105 28-29 0.44644821895782627 1105 30-31 0.244327532926782 1105 32-33 0.5732732492415664 1105 34-35 0.29619582077130246 1105 36-37 0.5096411454905976 1105 38-39 0.6128727123724076 1105 40-41 0.332283585242223 1105 42-43 0.6584859178555362 1105 44-45 0.332628676236574 1105 46-47 0.6951439834906452 1105 48-49 1.1583542836200351 1105 50-51 0.9620808008361834 1105 52-53 0.36296574186132347 1105 54-55 0.7369790074159397 1105 56-57 0.45676971770524943 1105 58-59 0.8754571741062946 1105 60-61 0.6936374191333954 1105 62-63 1.5226585802862118 1105 64-65 1.2247741242715975 1105 66-67 1.2997479242787335 1105 68-69 1.1913901361430916 1105 70-71 1.0429373325717783 1105 72-73 1.4039356500011415 1105 74-75 1.6584957681873256 1105 76-77 1.6758820976635462 1105 78-79 2.262914773999121 1105 80-81 2.0954754290741917 1105 82-83 2.226153296324185 1105 84-85 2.2060947706533014 1105 86-87 2.1776964470888167 1105 88-89 2.053399778482298 1105 90-91 0.9230124454577933 1105 92-93 1.732321874364235 1105 94-95 1.9997944444061382 1105 96-97 1.4811989524920257 1105 98-99 1.4627562424392657 1105 100-101 1.9881738830826805 1106 1 -0.18579963554644863 1106 2 -0.2757892412734506 1106 3 -0.7731644256686856 1106 4 -0.5358952845746217 1106 5 -0.881053759366857 1106 6 -1.04082594398443 1106 7 -1.1413855752808217 1106 8 -1.1653223845177791 1106 9 -0.6604917807721975 1106 10-11 -1.2503594761475654 1106 12-13 -1.9872616249754387 1106 14-15 -1.459122717389839 1106 16-17 -1.2382463283334744 1106 18-19 -0.9059503064050602 1106 20-21 -1.1937256022519165 1106 22-23 -1.3228682819736903 1106 24-25 -1.1887914601292806 1106 26-27 -1.6384439563028081 1106 28-29 -1.6962473207965374 1106 30-31 -1.1842842964203442 1106 32-33 -1.1833524779723987 1106 34-35 -2.079369790088421 1106 36-37 -1.492540496396927 1106 38-39 -1.4709588971944925 1106 40-41 -1.9204232667293368 1106 42-43 -2.4992791112330153 1106 44-45 -1.9984556805830707 1106 46-47 -1.2851404379702487 1106 48-49 -1.108492839844736 1106 50-51 -1.4142928255374514 1106 52-53 -1.848249513471579 1106 54-55 -1.319622479332601 1106 56-57 -1.437016642992873 1106 58-59 -1.322304622920207 1106 60-61 -1.2787688510669994 1106 62-63 -1.61446165242225 1106 64-65 -1.5388813379132742 1106 66-67 -0.9553021726831297 1106 68-69 -1.6905991980521264 1106 70-71 -2.0658215555988697 1106 72-73 -1.5311807042328631 1106 74-75 -1.365300288696968 1106 76-77 -1.0304446638102718 1106 78-79 -1.1727671910946107 1106 80-81 -1.7472039503698937 1106 82-83 -1.6631889790475043 1106 84-85 -1.5664892629601468 1106 86-87 -1.7383911417928921 1106 88-89 -1.7959861297271438 1106 90-91 -1.6042984789119572 1106 92-93 -1.7969686880145623 1106 94-95 -1.6486380054968954 1106 96-97 -0.830210226564212 1106 98-99 0.5560820342944659 1106 100-101 1.2325985760367821 1107 1 0.4021124523656354 1107 2 0.24304906327913756 1107 3 0.06494389458249117 1107 4 0.06889278449916247 1107 5 -0.07473507804817103 1107 6 0.3010107907094479 1107 7 -0.07604020636402709 1107 8 0.3189790283550593 1107 9 0.4772633212686159 1107 10-11 0.36453455838933735 1107 12-13 0.41648641270116826 1107 14-15 0.6553788524688784 1107 16-17 0.4198188208031013 1107 18-19 0.21473258213497104 1107 20-21 0.005744570432540286 1107 22-23 0.44803046213933584 1107 24-25 0.6334220406556454 1107 26-27 0.8294720562560656 1107 28-29 0.4038311721390997 1107 30-31 0.5942675089171772 1107 32-33 0.5297558422787816 1107 34-35 1.0678044642286864 1107 36-37 0.7329304612168883 1107 38-39 0.9168943210158673 1107 40-41 1.405812997006926 1107 42-43 0.6740921603525365 1107 44-45 0.850935999165749 1107 46-47 1.0315785573201808 1107 48-49 0.41495692467645995 1107 50-51 0.3549379436933222 1107 52-53 0.21650715842795165 1107 54-55 0.4008445536344283 1107 56-57 0.5308993695659936 1107 58-59 0.47959883076895693 1107 60-61 0.3121848381009755 1107 62-63 0.7648554590377188 1107 64-65 0.7544860090255057 1107 66-67 1.1442857394047792 1107 68-69 1.0932508804408059 1107 70-71 0.9538016783100822 1107 72-73 0.3289056379963391 1107 74-75 0.06395795306127283 1107 76-77 0.5234211132697908 1107 78-79 0.5921464666761906 1107 80-81 1.092474228594 1107 82-83 0.8425998749556385 1107 84-85 0.8531535941827109 1107 86-87 1.153386723199258 1107 88-89 1.3139039801629657 1107 90-91 1.2834566231288598 1107 92-93 1.3061514061769586 1107 94-95 1.7762050086318304 1107 96-97 1.9052685803431686 1107 98-99 2.1983504801343408 1107 100-101 2.193155875879796 1108 1 -0.6152245795345017 1108 2 -0.36503494702401085 1108 3 -0.9541763748845185 1108 4 -0.8445211247538609 1108 5 -0.9117669781868685 1108 6 -0.5675249731106433 1108 7 -0.6601495782681255 1108 8 -0.3733134226059107 1108 9 -0.5852490623255946 1108 10-11 -0.4586772506061152 1108 12-13 -0.06320449278724283 1108 14-15 -0.1254315299365132 1108 16-17 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-1.5385486711102487 2316 50-51 -1.810384636951838 2316 52-53 -1.5067482727767896 2316 54-55 -1.7068743403747817 2316 56-57 -2.013784633067317 2316 58-59 -1.9899535062949028 2316 60-61 -1.9014425483374673 2316 62-63 -1.4013723223974353 2316 64-65 -1.6275910153326265 2316 66-67 -1.6961520486136479 2316 68-69 -1.44912897472706 2316 70-71 -1.7962806126708308 2316 72-73 -2.3816769821484947 2316 74-75 -2.1843139363396453 2316 76-77 -1.752994291601837 2316 78-79 -1.5521096228563955 2316 80-81 -1.2509260347371374 2316 82-83 -1.9096219815288933 2316 84-85 -2.9717311984440187 2316 86-87 -2.113236054944256 2316 88-89 -2.2292987848139916 2316 90-91 -2.680911382137765 2316 92-93 -2.692285065185782 2316 94-95 -2.576987881427417 2316 96-97 -2.7126543952987063 2316 98-99 -2.8294638714127274 2316 100-101 -3.90139646118406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 634.0 3 104.0 4 112.0 5 113.0 6 113.0 7 142.0 8 148.0 9 197.0 10 220.0 11 288.0 12 426.0 13 651.0 14 722.0 15 966.0 16 1379.0 17 1892.0 18 1552.0 19 966.0 20 1035.0 21 989.0 22 1087.0 23 1220.0 24 1306.0 25 1319.0 26 1522.0 27 1637.0 28 1926.0 29 2247.0 30 2696.0 31 3321.0 32 4308.0 33 5891.0 34 8507.0 35 12815.0 36 20283.0 37 29763.0 38 24701.0 39 3697.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04711624663811 18.49785835242554 15.294636631423163 23.160388769513183 2 14.915999260281376 19.93257180249513 40.73217918261092 24.419249754612572 3 18.829663138727664 24.07818368043701 30.956242175941735 26.135911004893593 4 12.51191441537564 15.909834549670665 37.655954362454295 33.9222966724994 5 13.425945684492055 36.26961432859602 35.2830296046548 15.021410382257125 6 31.643274604325754 35.55166872858098 17.567509918518834 15.237546748574434 7 26.98156534597857 32.85392331809553 21.911146816058697 18.253364519867198 8 25.913023695604267 33.59045635188648 20.797105055488807 19.699414897020453 9 25.316707662121622 16.283252527263162 21.082564372342997 37.31747543827222 10-11 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25.898012001933964 30.667500924319558 21.957211114587185 36-37 21.159191565896762 25.731337129532868 31.078362373548877 22.03110893102149 38-39 20.991042229489548 26.27825963315797 30.81437508886677 21.91632304848571 40-41 20.980246483504374 26.46445113519624 30.711261672774842 21.84404070852455 42-43 20.767439793832757 26.517728605705145 30.323647027459344 22.391184573002754 44-45 21.04709361847344 26.448569559777045 30.054174724149696 22.45016209759982 46-47 21.610726803500715 26.70005758689578 29.683556452931597 22.005659156671904 48-49 21.47072206580781 26.93100702442909 29.160978869834768 22.437292039928334 50-51 21.13628365143635 27.050191430592633 29.577715132792754 22.235809785178258 52-53 21.515846353472334 27.140546401464654 28.89742085710731 22.446186387955706 54-55 21.18204536648312 27.020465040222675 29.006181814303844 22.79130777899036 56-57 21.313124491254012 26.91972743656004 29.004475187414023 22.762672884771924 58-59 21.559455299666936 26.39187570424663 29.31798712539411 22.730681870692322 60-61 20.81902649519788 26.36510340022891 30.099099291233905 22.716770813339306 62-63 20.824550240476047 26.203180042443226 30.247727652434797 22.724542064645934 64-65 20.95503161756081 26.182326156733527 30.13780919983933 22.724833025866328 66-67 20.344784687294506 26.045106367853272 30.0723336947056 23.537775250146623 68-69 20.63745388109676 25.612963766003883 30.540844109220938 23.20873824367842 70-71 20.756413948048532 25.69702916093437 30.015534960771557 23.531021930245537 72-73 20.24534861400428 25.073405518388704 30.46951093795545 24.211734929651563 74-75 20.558249321028907 25.172408889900037 30.418971376569452 23.8503704125016 76-77 20.594946967327324 25.021682258935936 30.219381806807515 24.163988966929224 78-79 19.90644472169793 24.346055002825853 30.477977897920955 25.269522377555266 80-81 20.13770647974037 24.173281246112122 31.109791947334557 24.57922032681295 82-83 19.990971039787855 24.528556752702467 31.249444576758606 24.231027630751075 84-85 19.49625174616377 24.424965609904348 30.348592938567027 25.730189705364854 86-87 19.639150339807205 24.345622316376147 30.33241391076862 25.68281343304803 88-89 19.50063087558423 22.90273853316984 30.894777061008334 26.701853530237603 90-91 19.486464968152866 21.608066992720655 32.582532415832574 26.3229356232939 92-93 19.12255009987986 21.777363882589626 32.1119080963112 26.988177921219314 94-95 17.544003070973613 22.4966588944495 32.88109929481347 27.07823873976342 96-97 17.22124283825474 23.005729396209784 34.00069662633816 25.772331139197313 98-99 18.090018233970635 25.958349772341943 31.13601546866281 24.815616525024613 100-101 17.706689414942776 26.607663325513613 27.954432359422764 27.731214900120847 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 167.0 1 95.5 2 31.0 3 38.0 4 32.0 5 32.5 6 48.5 7 94.0 8 159.0 9 203.0 10 192.5 11 184.0 12 207.5 13 248.0 14 276.5 15 291.5 16 306.0 17 346.0 18 395.5 19 406.0 20 428.0 21 487.0 22 553.0 23 622.0 24 724.5 25 839.5 26 1018.5 27 1183.0 28 1334.5 29 1470.0 30 1636.0 31 1909.5 32 2173.0 33 2310.5 34 2410.0 35 2606.5 36 2843.0 37 3112.5 38 3378.0 39 3633.5 40 3877.0 41 4094.0 42 4346.0 43 4620.0 44 4878.5 45 5086.5 46 5398.0 47 5899.5 48 6299.5 49 6150.5 50 5766.5 51 5476.0 52 5266.0 53 5266.5 54 5043.5 55 4517.0 56 4000.0 57 3516.5 58 3081.0 59 2578.0 60 2003.0 61 1565.5 62 1187.0 63 815.0 64 543.5 65 382.5 66 261.0 67 185.5 68 139.0 69 93.0 70 60.0 71 35.5 72 25.5 73 22.0 74 15.0 75 7.0 76 6.5 77 7.5 78 5.0 79 3.0 80 2.5 81 1.5 82 2.5 83 3.0 84 2.5 85 3.0 86 2.5 87 1.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 1.0 94 2.0 95 1.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2477021895737961 2 0.21363426665247168 3 0.21505376344086022 4 0.2193122538060258 5 0.2193122538060258 6 0.17672735015437027 7 0.16750062102984492 8 0.16750062102984492 9 0.1625323822704851 10-11 0.1905674438411583 12-13 0.18417970829341 14-15 0.17992121792824445 16-17 0.17424323077469037 18-19 0.1682103694240392 20-21 0.16998474040952483 22-23 0.1696298662124277 24-25 0.1646616274530679 26-27 0.1589836402995138 28-29 0.1646616274530679 30-31 0.1745981049717875 32-33 0.1696298662124277 34-35 0.17814684694275879 36-37 0.16182263387629084 38-39 0.16679087263565068 40-41 0.16785549522694204 42-43 0.16501650165016502 44-45 0.1696298662124277 46-47 0.16892011781823343 48-49 0.17246885978920473 50-51 0.17282373398630185 52-53 0.17566272756307888 54-55 0.17069448880371907 56-57 0.1635970048617765 58-59 0.1635970048617765 60-61 0.16182263387629084 62-63 0.16785549522694204 64-65 0.16288725646758223 66-67 0.16146775967919372 68-69 0.16040313708790233 70-71 0.17353348238049612 72-73 0.16892011781823343 74-75 0.17104936300081622 76-77 0.1611128854820966 78-79 0.16288725646758223 80-81 0.16572625004435926 82-83 0.16785549522694204 84-85 0.16288725646758223 86-87 0.1611128854820966 88-89 0.15437027573725115 90-91 0.15827389190531957 92-93 0.16040313708790233 94-95 0.15827389190531957 96-97 0.15401540154015403 98-99 0.1586287661024167 100-101 0.15969338869370808 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 140895.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.08968125759974 #Duplication Level Percentage of deduplicated Percentage of total 1 88.72845783551777 70.17505448689478 2 7.6599965785722395 12.11653375667166 3 1.785048541099619 4.235367604347369 4 0.7191683152409817 2.27515171291897 5 0.36346348576778054 1.437310561907495 6 0.2016933326673591 0.9571116835466674 7 0.1268064153514647 0.7020355280096301 8 0.06989324024204495 0.4422267274243342 9 0.055914585584422194 0.3980040076370459 >10 0.2616003776962933 3.8481616920262027 >50 0.016974070300726975 0.8504966429868752 >100 0.008986272512149576 1.385906785925688 >500 0.0019969494471443502 1.1766388097032907 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 835 0.5926399091522055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.46488519819723906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 400 0.28389935767770325 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 264 0.18737357606728414 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 210 0.14904716278079422 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 175 0.12420596898399516 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 164 0.11639873664785834 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 164 0.11639873664785834 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 150 0.10646225912913873 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 7.097483941942581E-4 0.0 24-25 0.0 0.0 0.0 0.0031938677738741613 0.0 26-27 0.0 0.0 0.0 0.005677987153554065 0.0 28-29 0.0 0.0 0.0 0.013130345292593775 0.0 30-31 0.0 0.0 0.0 0.03406792292132439 0.0 32-33 0.0 0.0 0.0 0.050392135987792325 0.0 34-35 0.0 0.0 0.0 0.0684907200397459 0.0 36-37 0.0 0.0 0.0 0.09013804606267078 0.0 38-39 0.0 0.0 0.0 0.11462436566237269 0.0 40-41 0.0 0.0 0.0 0.1426594272330459 0.0 42-43 0.0 0.0 0.0 0.20334291493665496 0.0 44-45 0.0 0.0 0.0 0.27751162212995495 0.0 46-47 0.0 0.0 0.0 0.3587778132651975 0.0 48-49 0.0 0.0 0.0 0.4666595691827248 0.0 50-51 0.0 0.0 0.0 0.5933496575463998 0.0 52-53 0.0 0.0 0.0 0.8641186699315093 0.0 54-55 0.0 0.0 0.0 1.063557968700096 0.0 56-57 0.0 0.0 0.0 1.5256041733205579 0.0 58-59 0.0 0.0 0.0 2.083821285354342 0.0 60-61 0.0 0.0 0.0 2.6232300649419784 0.0 62-63 0.0 0.0 0.0 3.593456119805529 0.0 64-65 0.0 0.0 0.0 4.1239930444657364 0.0 66-67 0.0 0.0 0.0 5.209908087582952 0.0 68-69 0.0 0.0 0.0 6.2482699882891515 0.0 70-71 0.0 0.0 0.0 7.223819156109159 0.0 72-73 0.0 0.0 0.0 9.111040136271692 0.0 74-75 0.0 0.0 0.0 10.756946662408176 0.0 76-77 0.0 0.0 0.0 11.862024912168636 0.0 78-79 0.0 0.0 0.0 13.23609780332872 0.0 80-81 0.0 0.0 0.0 15.512615777706802 0.0 82-83 0.0 0.0 0.0 16.818907697221334 0.0 84-85 0.0 0.0 0.0 18.732744242166152 0.0 86-87 0.0 0.0 0.0 22.363817026863977 0.0 88-89 0.0 0.0 0.0 27.258951701621775 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGCC 25 0.0050054607 57.019203 7 CTAAGGC 60 8.237289E-5 39.59667 3 GGTATCA 540 0.0 36.089798 1 GTATCAA 830 0.0 35.506508 1 CTTATAT 70 2.040171E-4 33.940002 2 TATATCT 60 0.0039259335 31.688606 4 GACAGGT 65 0.005817289 29.240616 7 TTATATC 65 0.005817289 29.240616 3 TCAACGC 1015 0.0 29.034878 4 TATCAAC 1000 0.0 28.98476 2 CAACGCA 1020 0.0 28.89255 5 ATCAACG 1025 0.0 28.741388 3 AACGCAG 1040 0.0 28.326845 6 CAATGCA 85 6.3664804E-4 27.95059 9 AATACAT 70 0.008338756 27.161661 5 ATACCAT 70 0.008353246 27.152 6 CTGTGCA 125 7.311577E-6 26.608961 9 CCACACT 90 8.872426E-4 26.40717 4 ACGCAGA 1130 0.0 26.070726 7 CTTGGAC 110 9.3972136E-5 25.91782 3 >>END_MODULE