FastQCFastQC Report
Thu 26 May 2016
SRR937306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937306_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140895
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.5181163277618085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.4492707335249654No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4640.3293232549061358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.28389935767770325No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC2130.151176407963377No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1920.13627169168529757No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.11072074949430427No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1480.1050427623407502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG1050.049.761535
ATAACAT405.372193E-447.4996453
AAGGTAA652.5607187E-643.8769761
TATAGAC500.001611064937.9997143
GTAAGGT1152.4701876E-937.1736374
ACTACAT550.002572074334.5451973
GGTAAGG1151.02550985E-733.0432323
CTCTAGA600.003925756631.6889291
TACATGG5400.030.7868082
GGTGTGC1252.1314008E-730.3997758
AGGTAAG953.4695448E-529.9997772
AGGTGTG1751.4915713E-1029.8569187
CTTAAGG650.00582711929.230553
TAGACTG650.00582711929.230555
GTACATG5700.028.3532521
TTATTGG1101.8189894E-1228.0679718-19
ACATGGG5250.028.047413
GTGTGGC1056.830825E-527.161941
TCTAAGA700.00836733727.1426542
GTGTGCA1754.8821676E-927.1426539