FastQCFastQC Report
Thu 26 May 2016
SRR937305_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937305_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209918
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.495431549462171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.3934869806305319No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.26677083432578436No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3210.15291685324745852No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3100.14767671185891634No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2700.12862165226421746No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2510.11957049895673548No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.10575558075057878No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2140.10194456883163901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG857.1486284E-944.722735
GTTTAAT500.001609818238.014321
GGTATCA6650.035.013191
GTATCAA9650.034.468941
TATACTA600.003936378831.67865
TATATAC1452.3715984E-829.493873
GGGCTAC650.005822584529.2417851
CTATACT856.3755084E-427.9517064
GACCACG700.00837055427.1466057
TATCAAC12350.026.9332222
ATCAACG12350.026.9332223
CAACGCA12450.026.7168925
TCAACGC12550.026.504014
GTTTAAG908.903439E-426.3988321
GTACATG5600.026.3045521
AACGCAG12850.025.879066
AATGTAT1151.2756749E-424.7860326
CGCAGAG13950.023.8384198
TATAATA1000.001645538323.7589512
ACGCAGA14000.023.753287
Produced by FastQC (version 0.11.5)