##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937305_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 209918 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.845263388561246 33.0 31.0 34.0 30.0 34.0 2 31.96822568812584 33.0 31.0 34.0 30.0 34.0 3 32.01002772511171 34.0 31.0 34.0 30.0 34.0 4 35.63747272744595 37.0 35.0 37.0 35.0 37.0 5 35.592007355253 37.0 35.0 37.0 33.0 37.0 6 35.665226421745636 37.0 35.0 37.0 35.0 37.0 7 35.60153012128546 37.0 35.0 37.0 33.0 37.0 8 35.606560657018456 37.0 35.0 37.0 33.0 37.0 9 37.257100391581474 39.0 37.0 39.0 34.0 39.0 10-11 37.17231728579731 39.0 37.0 39.0 34.0 39.0 12-13 37.068588687011115 39.0 37.0 39.0 33.5 39.0 14-15 38.26798559437495 40.0 38.0 41.0 33.5 41.0 16-17 38.20692841966863 40.0 38.0 41.0 34.0 41.0 18-19 38.16313512895512 40.0 38.0 41.0 33.5 41.0 20-21 38.06549462170943 40.0 38.0 41.0 33.5 41.0 22-23 37.956421078706924 40.0 38.0 41.0 33.0 41.0 24-25 37.81251012300041 40.0 38.0 41.0 33.0 41.0 26-27 37.65062786421365 40.0 37.0 41.0 33.0 41.0 28-29 37.4618994083404 40.0 37.0 41.0 32.0 41.0 30-31 37.41350432073476 39.5 37.0 41.0 32.5 41.0 32-33 37.23534904105412 39.0 36.5 41.0 32.0 41.0 34-35 37.038303051667796 39.0 36.0 41.0 31.5 41.0 36-37 37.065385055116764 39.0 36.0 41.0 31.0 41.0 38-39 37.03185053211254 40.0 36.0 41.0 31.0 41.0 40-41 36.86217713583399 39.5 36.0 41.0 31.0 41.0 42-43 36.60039396335712 39.0 36.0 41.0 30.5 41.0 44-45 36.3235168017988 39.0 36.0 41.0 30.0 41.0 46-47 35.93408140321459 39.0 35.0 41.0 29.0 41.0 48-49 35.56960575081699 39.0 35.0 41.0 27.5 41.0 50-51 34.59251945997961 38.0 34.0 39.5 25.5 40.5 52-53 34.68438390228566 38.0 34.5 39.5 26.0 40.5 54-55 35.016913747272746 39.0 35.0 40.0 26.0 41.0 56-57 34.74992139787918 38.5 34.5 40.0 25.0 41.0 58-59 34.4717770748578 38.0 34.0 40.0 24.0 41.0 60-61 34.15219037910041 38.0 34.0 40.0 23.0 41.0 62-63 33.81517783134367 37.5 34.0 40.0 22.0 41.0 64-65 33.40741384731181 37.0 33.5 40.0 20.5 41.0 66-67 32.9912037081146 36.0 33.0 39.0 19.0 41.0 68-69 32.46008679579646 36.0 32.5 39.0 15.5 40.5 70-71 31.982324050343465 35.0 32.0 38.5 10.0 40.0 72-73 31.56768357168037 35.0 31.5 37.5 7.0 40.0 74-75 31.11090521060605 35.0 31.0 37.0 3.5 39.0 76-77 30.677402604826646 35.0 31.0 36.5 2.0 39.0 78-79 30.23465114949647 35.0 30.5 36.0 2.0 39.0 80-81 29.81664268905001 34.0 30.0 36.0 2.0 37.5 82-83 29.374596270924837 34.0 29.5 35.0 2.0 37.0 84-85 28.964324164673826 34.0 29.0 35.0 2.0 36.5 86-87 28.572959441305652 34.0 29.0 35.0 2.0 36.0 88-89 28.203834354366943 34.0 29.0 35.0 2.0 36.0 90-91 27.76929086595718 34.0 27.5 35.0 2.0 35.0 92-93 27.340230470945798 34.0 26.5 35.0 2.0 35.0 94-95 26.843646090378147 33.0 25.5 35.0 2.0 35.0 96-97 26.288400708848215 33.0 25.0 35.0 2.0 35.0 98-99 25.564872950390154 33.0 22.0 35.0 2.0 35.0 100-101 23.7368829733515 30.5 9.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2270811958729304 1101 2 -0.05974339528524908 1101 3 0.18690008465944175 1101 4 0.2648687069144984 1101 5 0.13212845751078817 1101 6 -0.06770242339575105 1101 7 0.21243558126931816 1101 8 0.0703063817227445 1101 9 -0.18457339302317877 1101 10-11 0.17630651784890006 1101 12-13 0.5149397360382295 1101 14-15 0.4064744709040653 1101 16-17 0.3540757070605096 1101 18-19 0.19182265189575531 1101 20-21 0.1449058847843787 1101 22-23 0.11766272192939198 1101 24-25 0.3634787497886407 1101 26-27 0.8479060271681789 1101 28-29 0.7248103283200678 1101 30-31 0.6831301750059282 1101 32-33 0.86882694239101 1101 34-35 0.7635743690135612 1101 36-37 0.4950544965834709 1101 38-39 0.23523370099587737 1101 40-41 0.25777651540107627 1101 42-43 0.52242702802004 1101 44-45 0.6826890467234463 1101 46-47 0.7196810898362216 1101 48-49 0.8912024642202141 1101 50-51 0.34935004284874793 1101 52-53 0.5245916006144 1101 54-55 0.9933248979631628 1101 56-57 0.9163978464797466 1101 58-59 0.875322319278034 1101 60-61 0.8493448801022581 1101 62-63 0.6391472349100908 1101 64-65 0.9056032773917906 1101 66-67 0.9038084557198331 1101 68-69 0.9650270047799481 1101 70-71 0.778563030536759 1101 72-73 0.9690458241105766 1101 74-75 0.8892341471406873 1101 76-77 0.6906899584180337 1101 78-79 0.5618684539888825 1101 80-81 1.0439998213950368 1101 82-83 0.8818224440624505 1101 84-85 0.9467461609737207 1101 86-87 1.2217846678408648 1101 88-89 0.9787029807772036 1101 90-91 0.7262024171744166 1101 92-93 0.6894266621348706 1101 94-95 0.7415407381520325 1101 96-97 0.4285834422562367 1101 98-99 0.5531464066006286 1101 100-101 0.4879648231820717 1102 1 0.13696422284833787 1102 2 -0.0662485277751017 1102 3 -0.19457520777311998 1102 4 -0.26067438212823646 1102 5 -0.11566337824953621 1102 6 0.17292779861427476 1102 7 -0.06268974697131569 1102 8 0.06529325209753267 1102 9 0.44439533459677705 1102 10-11 0.27707639132705 1102 12-13 0.32077048323326096 1102 14-15 0.35544797293319164 1102 16-17 0.15599740675016704 1102 18-19 0.2604547836694451 1102 20-21 0.3632157439393069 1102 22-23 0.5926865939752233 1102 24-25 0.46950047335035094 1102 26-27 0.5384944730979981 1102 28-29 0.2832670005568758 1102 30-31 0.3284810940796987 1102 32-33 0.5567894632790598 1102 34-35 0.5169164554303691 1102 36-37 0.1760344440649746 1102 38-39 0.5407063675034038 1102 40-41 0.30149219933519333 1102 42-43 0.5765270518920858 1102 44-45 0.4930554826270068 1102 46-47 0.6249985880458198 1102 48-49 1.0148298215847404 1102 50-51 0.9361547695138199 1102 52-53 1.0559236607719598 1102 54-55 0.9990243489061044 1102 56-57 1.4670662637631082 1102 58-59 1.327906468239597 1102 60-61 1.1384651952132643 1102 62-63 1.2723830907229328 1102 64-65 1.4017837500583 1102 66-67 1.4040770162354619 1102 68-69 1.9064509723139622 1102 70-71 1.5270411876148131 1102 72-73 1.3396891757458143 1102 74-75 1.3651532209053094 1102 76-77 1.4266949715476578 1102 78-79 1.425588912332163 1102 80-81 1.0480879092441633 1102 82-83 1.3407625252274045 1102 84-85 1.3661481662255746 1102 86-87 1.0989629920232495 1102 88-89 1.410995890779592 1102 90-91 1.1489166687857768 1102 92-93 0.7411394814234811 1102 94-95 0.4245796495867431 1102 96-97 0.8146839435692002 1102 98-99 0.7976259960014396 1102 100-101 1.022698649871021 1103 1 0.21182297991049026 1103 2 0.2951568394565385 1103 3 0.4270702724528661 1103 4 0.44774722804125844 1103 5 0.20460498333238064 1103 6 0.35857398505495297 1103 7 0.46096844511907875 1103 8 0.44391260238002417 1103 9 0.3115386961786939 1103 10-11 0.5740838065812923 1103 12-13 0.5737718956626452 1103 14-15 0.41256308592754465 1103 16-17 0.6300440169196619 1103 18-19 0.28927717067509207 1103 20-21 0.5715490772726355 1103 22-23 0.9071286843707114 1103 24-25 0.7328302756102403 1103 26-27 0.6868878346799221 1103 28-29 0.44000252315574784 1103 30-31 0.5414048228932558 1103 32-33 0.5306153813581531 1103 34-35 0.43724661079760097 1103 36-37 0.6130489214660955 1103 38-39 0.46770142400058035 1103 40-41 0.40742440272502023 1103 42-43 0.4413310773158159 1103 44-45 0.5186999035309654 1103 46-47 0.5237450569723663 1103 48-49 0.3250346238446298 1103 50-51 0.539747636745453 1103 52-53 0.6153339715064163 1103 54-55 0.4653025974936753 1103 56-57 0.6985281281698903 1103 58-59 0.9235544202169947 1103 60-61 1.0372116641398108 1103 62-63 1.3356334438302753 1103 64-65 1.0597992867814554 1103 66-67 1.1446755331846177 1103 68-69 1.2556299977376923 1103 70-71 1.1662537582362873 1103 72-73 1.592028511904008 1103 74-75 1.8227362011312955 1103 76-77 1.686581977197374 1103 78-79 1.362117867134419 1103 80-81 1.932754222803485 1103 82-83 2.3988045450014184 1103 84-85 2.0565613018187925 1103 86-87 2.020970407277485 1103 88-89 2.111075339932132 1103 90-91 1.7311906800852128 1103 92-93 1.825796967299187 1103 94-95 1.3962869235980442 1103 96-97 1.4182768108008403 1103 98-99 1.283413425379969 1103 100-101 1.814586008628087 1104 1 -0.10177351131758172 1104 2 0.1641261377021479 1104 3 0.22257465841778412 1104 4 0.06370117540967613 1104 5 0.15964884298150395 1104 6 0.13735687979179545 1104 7 -0.13004032681074307 1104 8 0.1936384795730035 1104 9 0.18681281898571456 1104 10-11 -0.03899185131344751 1104 12-13 -0.06493424468823861 1104 14-15 0.019196857857366467 1104 16-17 0.08892559586703186 1104 18-19 -0.2585572591494625 1104 20-21 -0.13020530869226832 1104 22-23 0.08421201770403997 1104 24-25 0.17718334578567863 1104 26-27 0.07696458906588077 1104 28-29 -0.10700953824775894 1104 30-31 -0.15596359815937433 1104 32-33 -0.13701071513307994 1104 34-35 -0.04781369621994003 1104 36-37 -0.38105743818302074 1104 38-39 0.0617255468075939 1104 40-41 0.5554507185144928 1104 42-43 0.5025975246842407 1104 44-45 0.7307338947590338 1104 46-47 0.8195235657442979 1104 48-49 0.9494863782305885 1104 50-51 0.02179259288580937 1104 52-53 0.2027791469450122 1104 54-55 0.7026929483708741 1104 56-57 0.37122549659094517 1104 58-59 0.21645463951524135 1104 60-61 0.4547006992275371 1104 62-63 0.19075954032150122 1104 64-65 0.9189001639744419 1104 66-67 0.6245275068688301 1104 68-69 -0.0161627654201979 1104 70-71 0.10019016174505779 1104 72-73 0.7843256610268092 1104 74-75 0.3941451923593675 1104 76-77 0.43109952105702476 1104 78-79 0.4956156741519635 1104 80-81 0.16918514385611516 1104 82-83 0.0255863432470278 1104 84-85 0.09027840708195001 1104 86-87 -0.1874999874593577 1104 88-89 -0.7010134758573408 1104 90-91 -0.6646426532481193 1104 92-93 -0.5905955765604602 1104 94-95 -0.39962864657739416 1104 96-97 -0.1062571804272352 1104 98-99 -0.33802297812880155 1104 100-101 -0.44925719312630363 1105 1 0.541533124838022 1105 2 0.5908995930797261 1105 3 0.5769796927427251 1105 4 0.4266874454325631 1105 5 0.6765252731874583 1105 6 0.5426772459245157 1105 7 0.6394104741045865 1105 8 0.6819107907858211 1105 9 1.0083231164685529 1105 10-11 0.9385539152769482 1105 12-13 0.9682465333438017 1105 14-15 1.0622460569420369 1105 16-17 0.865098364745748 1105 18-19 0.8417001779214743 1105 20-21 0.9066940048088696 1105 22-23 0.8179076698779468 1105 24-25 0.9350494785087875 1105 26-27 1.135030950621946 1105 28-29 0.8409309651847323 1105 30-31 0.5930352576758651 1105 32-33 -0.32751414762735465 1105 34-35 -0.48474161384007886 1105 36-37 -0.131629520562889 1105 38-39 -0.7933945904921771 1105 40-41 -0.40235820597063565 1105 42-43 -0.08550316181462136 1105 44-45 -0.36637708922265944 1105 46-47 -0.3388002328826971 1105 48-49 -0.269621173256823 1105 50-51 -0.1566971096313523 1105 52-53 -0.10500117342111537 1105 54-55 0.3126757858994793 1105 56-57 0.38738682382206235 1105 58-59 0.9214031521010568 1105 60-61 1.060535939502138 1105 62-63 0.8540890597723063 1105 64-65 0.35010725779595475 1105 66-67 0.9500423810107037 1105 68-69 0.7552903238246458 1105 70-71 0.7085997727290376 1105 72-73 0.391734127846032 1105 74-75 0.6638820344646312 1105 76-77 0.20469791922636205 1105 78-79 -0.06473901692355 1105 80-81 -0.16948762502260095 1105 82-83 0.13442501601591061 1105 84-85 0.7284363018187925 1105 86-87 0.6680492116253127 1105 88-89 0.7948389268886551 1105 90-91 0.031009520664927237 1105 92-93 0.9102625470093315 1105 94-95 0.7338095685255759 1105 96-97 0.863136412250114 1105 98-99 0.8606326282785233 1105 100-101 0.6595814796425792 1106 1 -0.21361561658073924 1106 2 0.09833666401794261 1106 3 0.07125886894409916 1106 4 0.12291170172547083 1106 5 -0.004824841229023491 1106 6 0.07814635347600785 1106 7 -0.22214558996864042 1106 8 -0.0497825730585717 1106 9 0.028918082143611912 1106 10-11 0.010350253949717114 1106 12-13 -0.2688816131092864 1106 14-15 -0.07619787898473618 1106 16-17 -0.3091007199224407 1106 18-19 0.0013111619031676014 1106 20-21 -0.2552053086922683 1106 22-23 -0.11315640334859012 1106 24-25 0.4304728194698839 1106 26-27 -0.08750909514463956 1106 28-29 -0.11358848561617663 1106 30-31 0.12364166499851592 1106 32-33 -0.24885282039623746 1106 34-35 -0.14320843306204978 1106 36-37 -0.150794280288288 1106 38-39 0.01238344154443638 1106 40-41 -0.28336507095918506 1106 42-43 -0.09608668584207436 1106 44-45 -1.5422924210304458 1106 46-47 -1.4863974868872774 1106 48-49 -2.1755136217694115 1106 50-51 -2.1755758281668207 1106 52-53 -2.040641905686563 1106 54-55 -2.261122841102818 1106 56-57 -3.4478534507774796 1106 58-59 -3.740782202590019 1106 60-61 -3.5781940376145727 1106 62-63 -2.776345722836389 1106 64-65 -3.0843893097097705 1106 66-67 -2.862314598394331 1106 68-69 -3.1082680285780953 1106 70-71 -2.7287572066759935 1106 72-73 -2.593963812657403 1106 74-75 -2.4216442813248413 1106 76-77 -2.2366636368377115 1106 78-79 -2.6951737995322453 1106 80-81 -2.1202885403544123 1106 82-83 -2.3658610251740235 1106 84-85 -2.100511066602259 1106 86-87 -1.6480263032488303 1106 88-89 -1.9279871600678682 1106 90-91 -1.7699058111428556 1106 92-93 -1.7748061028762514 1106 94-95 -2.1068654886826614 1106 96-97 -2.1259940225324954 1106 98-99 -1.6571019254972228 1106 100-101 -0.8670203510210364 1107 1 0.4534896465771574 1107 2 0.0930735061232042 1107 3 -0.04584639421379677 1107 4 0.09462222804125986 1107 5 0.3129383166657149 1107 6 0.29919898505495013 1107 7 -0.5681982215475827 1107 8 0.0074542690466898875 1107 9 0.12299702951202818 1107 10-11 0.3787713065812923 1107 12-13 -0.03664477100402053 1107 14-15 0.09485475259420895 1107 16-17 0.41129401691966194 1107 18-19 0.13354800400842493 1107 20-21 0.2069657439393069 1107 22-23 -0.23453798229595435 1107 24-25 0.2442886089435632 1107 26-27 -0.007903831986752152 1107 28-29 -0.03760164351091788 1107 30-31 -0.13984517710674993 1107 32-33 -0.40740545197517974 1107 34-35 0.3992257774642667 1107 36-37 0.4031530881327683 1107 38-39 0.7291597573339104 1107 40-41 0.3324244027250174 1107 42-43 -0.10398142268418553 1107 44-45 -0.16827926313570885 1107 46-47 -0.3814632763609609 1107 48-49 -0.38538204282204447 1107 50-51 -0.49827319658787417 1107 52-53 -0.6669576951602423 1107 54-55 -0.6367807358396504 1107 56-57 -0.41397187183010686 1107 58-59 -0.08321641311633243 1107 60-61 -0.26799666919351495 1107 62-63 -0.37634572283639045 1107 64-65 -0.6235340465518675 1107 66-67 -0.047511966815385165 1107 68-69 0.25719249773769093 1107 70-71 0.1922954249029516 1107 72-73 -0.18870065476266262 1107 74-75 -0.7673679655353673 1107 76-77 0.5261653105307111 1107 78-79 0.5485762004677532 1107 80-81 0.1468167228034858 1107 82-83 0.3545337116680827 1107 84-85 0.5534363018187918 1107 86-87 0.5069079072774834 1107 88-89 -0.3717371600678696 1107 90-91 -0.09589265324811791 1107 92-93 -0.09191136603414662 1107 94-95 0.3832660902647085 1107 96-97 -0.3009940225324961 1107 98-99 -0.3426282412866968 1107 100-101 -0.4703098247052502 1108 1 -0.45749761456934124 1108 2 -0.12794560215705175 1108 3 -0.3202094515386378 1108 4 -0.4826866891561963 1108 5 -0.4521094540349182 1108 6 -0.5469793588940917 1108 7 -0.1909689221845312 1108 8 -0.1645998710807035 1108 9 -0.18026730169815863 1108 10-11 -0.6092860182594748 1108 12-13 -0.27701101304224096 1108 14-15 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-0.04847728091728243 2316 50-51 -0.02833272039740109 2316 52-53 -0.14731483801738676 2316 54-55 -0.4617807358396533 2316 56-57 0.0035876519794157957 2316 58-59 -0.36327593692585936 2316 60-61 -0.8593657168125688 2316 62-63 -1.5531314371221043 2316 64-65 -0.5369269036947344 2316 66-67 -0.4978095858630027 2316 68-69 -0.9121527403575485 2316 70-71 -1.016037908430384 2316 72-73 -1.274414940476948 2316 74-75 -1.0236179655353688 2316 76-77 -0.7955608799454801 2316 78-79 -0.37672141857986574 2316 80-81 -0.5430642295774675 2316 82-83 -0.3329662883319173 2316 84-85 -0.8310875077050177 2316 86-87 -0.9332706641510846 2316 88-89 -0.3148919219726274 2316 90-91 -0.24797598658145503 2316 92-93 -0.1258399374627146 2316 94-95 -0.21494819544957622 2316 96-97 -0.5039702130086852 2316 98-99 -0.8765568127152648 2316 100-101 -0.5250717294671539 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 936.0 3 179.0 4 146.0 5 163.0 6 162.0 7 191.0 8 246.0 9 273.0 10 328.0 11 483.0 12 684.0 13 852.0 14 1020.0 15 1376.0 16 1874.0 17 2531.0 18 2076.0 19 1461.0 20 1330.0 21 1542.0 22 1554.0 23 1717.0 24 1991.0 25 2052.0 26 2314.0 27 2812.0 28 3045.0 29 3699.0 30 4489.0 31 5545.0 32 7244.0 33 9706.0 34 13763.0 35 20489.0 36 30586.0 37 42967.0 38 33414.0 39 4678.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0478282671506 18.497576350915736 15.305045488192173 23.149549893741494 2 15.095744833216369 19.680802814816936 40.520760229730314 24.702692122236385 3 18.94390069228933 23.605633802816904 30.718071138696583 26.73239436619718 4 12.61142543388479 15.768817589343264 37.22600205304495 34.393754923726995 5 13.531835259632691 36.10749084590083 35.247220352414914 15.11345354205157 6 32.113134436618374 35.554696596613795 17.268892324724654 15.063276642043178 7 27.517454318124337 32.58600695786625 21.46105646943168 18.43548225457773 8 25.89229043880789 34.10484621441695 20.473883891288267 19.52897945548689 9 25.678726013734387 16.03649777854133 20.838761709783483 37.446014497940794 10-11 24.704641501239657 25.668210572533745 27.96784285090451 21.659305075322084 12-13 25.43215768625018 23.2129050732592 28.768434114446617 22.586503126044004 14-15 22.497804882704383 24.56145374028899 26.84890912214396 26.091832254862663 16-17 21.60122936541646 27.905306410739765 27.66955077575058 22.823913448093194 18-19 21.568126889298725 26.23462018385397 30.335665903988435 21.861587022858874 20-21 22.115141421420084 25.311976559405586 30.37303568105139 22.19984633812294 22-23 21.79308534752213 25.202930018372268 30.567392808570542 22.436591825535064 24-25 21.95115549977811 25.30837981895659 30.546899977572377 22.19356470369292 26-27 21.58879686564208 25.443035860433266 30.59745118671811 22.370716087206542 28-29 21.323624147622887 26.042059352646724 30.63928879896546 21.995027700764933 30-31 21.539999618233182 25.701973734443 30.140490188592807 22.617536458731006 32-33 21.27572762716475 25.743136515693077 30.246097608696687 22.735038248445484 34-35 21.911862950944837 25.61987020423745 30.06036457339187 22.407902271425844 36-37 21.749722035321795 25.57704512810235 30.22895700017656 22.444275836399296 38-39 21.468210795541303 26.034986257443887 30.08259848831883 22.414204458695984 40-41 21.50648718548245 26.26152972557702 30.069381152566958 22.162601936373573 42-43 21.464529582142397 26.17037825250872 29.585482585687767 22.779609579661113 44-45 21.520026342287483 26.188624140415843 29.438699301833935 22.852650215462734 46-47 21.978575177744904 26.504509233191776 29.161616643603573 22.35529894545975 48-49 21.92492698848995 26.661608352898508 28.6644142854416 22.74905037316994 50-51 21.63001775181813 26.859646108915996 28.96624291358873 22.544093225677145 52-53 21.929895726454937 26.96867021403517 28.358109236679475 22.74332482283042 54-55 21.701667688739462 26.947645215864164 28.444007511638507 22.90667958375787 56-57 21.639166698476924 26.677673015994195 28.512091079131196 23.17106920639768 58-59 21.986263952536923 26.32607248758913 28.73930375081407 22.948359809059877 60-61 21.40299312197461 26.26747240320545 29.430696080980816 22.89883839383912 62-63 21.330928558136872 26.20656255516907 29.58994765796844 22.872561228725612 64-65 21.282245193489054 26.219371091988712 29.51540063601728 22.982983078504954 66-67 20.96325864491946 26.07350179781876 29.313631689329284 23.649607867932495 68-69 21.27864644218703 25.673367337688052 29.77464560241625 23.273340617708666 70-71 21.269113405210156 25.8164874517766 29.29787685825601 23.61652228475723 72-73 20.906188558230095 25.62587092217535 29.368927405654077 24.099013113940483 74-75 20.91687741566064 25.58548456363029 29.564346041895313 23.933291978813763 76-77 21.033493827572876 25.423623670434864 29.600211871483722 23.942670630508537 78-79 20.675192059932243 24.86806317698144 29.58701149973756 24.869733263348763 80-81 20.915796705918275 24.85065938889631 29.92179895795561 24.3117449472298 82-83 20.699528287255646 24.924184762509217 30.235904092233323 24.140382858001818 84-85 20.36744129456138 24.991590504068764 29.49788748690862 25.14308071446123 86-87 20.416927106941944 24.942146473201294 29.49933438622776 25.141592033629003 88-89 20.260627041367744 24.000400817859298 29.722552923465262 26.016419217307696 90-91 20.30608471604261 23.050827239869744 30.927439075304473 25.715648968783178 92-93 20.19644846746814 22.930598864826564 30.759191970282217 26.11376069742308 94-95 18.846150175599327 23.455871125362652 31.4582856161246 26.239693082913423 96-97 18.593227374880115 23.60851993758917 32.27040877187123 25.52784391565949 98-99 19.21793174199859 25.7531403213685 30.226055422347876 24.80287251428503 100-101 18.82365570876473 26.12505367622501 28.466529891693305 26.584760723316954 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 252.0 1 145.0 2 44.0 3 45.5 4 36.5 5 42.5 6 74.5 7 130.5 8 221.5 9 288.5 10 262.0 11 248.5 12 288.0 13 312.5 14 331.0 15 359.5 16 378.5 17 406.5 18 461.5 19 511.0 20 565.5 21 643.5 22 728.0 23 834.0 24 972.5 25 1136.0 26 1315.0 27 1545.0 28 1816.5 29 2030.0 30 2234.5 31 2599.0 32 3020.5 33 3225.0 34 3420.5 35 3724.5 36 4055.5 37 4453.0 38 4943.0 39 5342.0 40 5675.5 41 6025.0 42 6307.0 43 6783.0 44 7220.5 45 7455.0 46 7937.0 47 8616.5 48 9295.0 49 9353.5 50 8672.5 51 8229.0 52 8090.0 53 8016.5 54 7784.0 55 7134.0 56 6293.0 57 5526.5 58 4999.5 59 4262.5 60 3344.0 61 2664.5 62 2027.0 63 1465.5 64 1018.0 65 705.0 66 509.0 67 367.0 68 259.5 69 184.5 70 125.5 71 74.5 72 55.0 73 38.0 74 24.0 75 17.0 76 12.5 77 8.5 78 7.5 79 3.5 80 2.5 81 2.5 82 1.0 83 2.0 84 2.0 85 1.0 86 0.5 87 0.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2491449042006879 2 0.2167513028896998 3 0.22294419725797693 4 0.22532607970731428 5 0.21389304395049497 6 0.17244828933202488 7 0.17673567774083215 8 0.17768843072056706 9 0.17673567774083215 10-11 0.183166760354043 12-13 0.18483407806857916 14-15 0.17244828933202488 16-17 0.18054668965977191 18-19 0.1664935832086815 20-21 0.17483017178136223 22-23 0.17292466582189236 24-25 0.16911365390295258 26-27 0.17602111300603093 28-29 0.17006640688268754 30-31 0.17435379529149478 32-33 0.17483017178136223 34-35 0.170542783372555 36-37 0.17101915986242247 38-39 0.16673177145361523 40-41 0.16720814794348268 42-43 0.16625539496374775 44-45 0.1757829247610972 46-47 0.16577901847388027 48-49 0.17244828933202488 50-51 0.17244828933202488 52-53 0.17768843072056706 54-55 0.1793557484351032 56-57 0.16292075953467544 58-59 0.15410779447212722 60-61 0.16077706533027183 62-63 0.15958612410560313 64-65 0.15839518288093446 66-67 0.17078097161748873 68-69 0.1605388770853381 70-71 0.16506445373907908 72-73 0.17625930125096465 74-75 0.16577901847388027 76-77 0.17006640688268754 78-79 0.16577901847388027 80-81 0.1572042416562658 82-83 0.17268647757695862 84-85 0.1588715593708019 86-87 0.1605388770853381 88-89 0.16506445373907908 90-91 0.15982431235053687 92-93 0.16506445373907908 94-95 0.16673177145361523 96-97 0.16149163006507303 98-99 0.1645880772492116 100-101 0.15625148867653085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 209918.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.29716679868714 #Duplication Level Percentage of deduplicated Percentage of total 1 88.37547372208925 69.19549206931416 2 7.638670512954887 11.961725185460836 3 1.8788270922624393 4.413205146862937 4 0.82443443376706 2.5820352150096277 5 0.4054559741496886 1.5873027018761174 6 0.2337077479869224 1.0979192715766564 7 0.14958449413558272 0.8198429461481672 8 0.1129315124152017 0.7073773971520844 9 0.06067712438058018 0.42757622356418756 >10 0.2923391965671827 3.949994413259134 >50 0.020013918404573883 1.1173420915835481 >100 0.006064823758961783 0.9881805748344087 >500 0.0012129647517923564 0.658153905199659 >1k 6.064823758961782E-4 0.49385285815847424 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1040 0.495431549462171 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 826 0.3934869806305319 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 560 0.26677083432578436 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 321 0.15291685324745852 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 310 0.14767671185891634 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 270 0.12862165226421746 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 251 0.11957049895673548 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 222 0.10575558075057878 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 214 0.10194456883163901 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 9.527529797349441E-4 0.0 24-25 0.0 0.0 0.0 0.0019055059594698881 0.0 26-27 0.0 0.0 0.0 0.0038110119189397767 0.0 28-29 0.0 0.0 0.0 0.00905115330748197 0.0 30-31 0.0 0.0 0.0 0.026915271677512172 0.0 32-33 0.0 0.0 0.0 0.04144475461847007 0.0 34-35 0.0 0.0 0.0 0.055021484579693025 0.0 36-37 0.0 0.0 0.0 0.07479110890919312 0.0 38-39 0.0 0.0 0.0 0.09432254499375947 0.0 40-41 0.0 0.0 0.0 0.11528311054792824 0.0 42-43 0.0 0.0 0.0 0.17292466582189236 0.0 44-45 0.0 0.0 0.0 0.2458102687716156 0.0 46-47 0.0 0.0 0.0 0.31226478910812794 0.0 48-49 0.0 0.0 0.0 0.3901523452014596 0.0 50-51 0.0 0.0 0.0 0.5021008203203156 0.0 52-53 0.0 0.0 0.0 0.7267123352928286 0.0 54-55 0.0 0.0 0.0 0.887013024133233 0.0 56-57 0.0 0.0 0.0 1.2550138625558551 0.0 58-59 0.0 0.0 0.0 1.7039987042559477 0.0 60-61 0.0 0.0 0.0 2.152030792976305 0.0 62-63 0.0 0.0 0.0 2.887556093331682 0.0 64-65 0.0 0.0 0.0 3.3170094989472076 0.0 66-67 0.0 0.0 0.0 4.123514896292838 0.0 68-69 0.0 0.0 0.0 4.952648176907173 0.0 70-71 0.0 0.0 0.0 5.747244162006117 0.0 72-73 0.0 0.0 0.0 7.120875770538973 0.0 74-75 0.0 0.0 0.0 8.396612010404063 0.0 76-77 0.0 0.0 0.0 9.337455577892321 0.0 78-79 0.0 0.0 0.0 10.355233948494174 0.0 80-81 0.0 0.0 0.0 12.05018149944264 0.0 82-83 0.0 0.0 0.0 13.046284739755524 0.0 84-85 0.0 0.0 0.0 14.519717222915615 0.0 86-87 0.0 0.0 0.0 17.1736106479673 0.0 88-89 0.0 0.0 0.0 20.638296858773426 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 85 7.1486284E-9 44.72273 5 GTTTAAT 50 0.0016098182 38.01432 1 GGTATCA 665 0.0 35.01319 1 GTATCAA 965 0.0 34.46894 1 TATACTA 60 0.0039363788 31.6786 5 TATATAC 145 2.3715984E-8 29.49387 3 GGGCTAC 65 0.0058225845 29.241785 1 CTATACT 85 6.3755084E-4 27.951706 4 GACCACG 70 0.008370554 27.146605 7 TATCAAC 1235 0.0 26.933222 2 ATCAACG 1235 0.0 26.933222 3 CAACGCA 1245 0.0 26.716892 5 TCAACGC 1255 0.0 26.50401 4 GTTTAAG 90 8.903439E-4 26.398832 1 GTACATG 560 0.0 26.304552 1 AACGCAG 1285 0.0 25.87906 6 AATGTAT 115 1.2756749E-4 24.786032 6 CGCAGAG 1395 0.0 23.838419 8 TATAATA 100 0.0016455383 23.758951 2 ACGCAGA 1400 0.0 23.75328 7 >>END_MODULE