FastQCFastQC Report
Thu 26 May 2016
SRR937305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209918
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.46923084251946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.4163530521441706No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6500.3096447184138569No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.28058575253194107No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC2730.13005078173381987No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.12242875789594031No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2280.10861383968978364No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATGG459.588703E-442.2386362
ATAAGAC651.3214607E-436.552673
GTCTTAG958.5965075E-735.0303081
GTGTTAT550.002563933434.5753671
CTATAAC702.0429652E-433.941763
AGACCGT702.0486953E-433.925596
GACCGTG650.0058358429.2282057
GGTATCA14100.028.9967171
GTGTAAG856.3573336E-427.965371
TAACTCC700.0083603527.153414
GCCTAGC350.00838432827.14370594-95
GTGTAGA1457.7771074E-726.2295881
GTACTGA1601.8336468E-623.7705651
AGGTAAG1000.001645431323.7592352
TAAAGCC1000.001645431323.7592354
GCCCCTT1000.001650004123.7479156
ACAGCTC1401.7717111E-523.7479138
GAGTACT10700.023.08208712-13
ATTATTC1252.24147E-422.8088673
GTATCAA17950.022.766512