FastQCFastQC Report
Thu 26 May 2016
SRR937304_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937304_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290794
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12660.43535973919681975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.350763770916869No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.2046121997015069No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4740.16300198766136853No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4050.1392738502169921No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3600.12379897797065965No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3450.11864068722188216No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3190.10969964992400118No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA550.002576391734.5456439
GTATCAA18050.031.321361
GTATTAC804.480783E-429.692781
AACCGCT650.005836812329.2309287
CTATAAG856.3889E-427.9461461
TAGGGGA856.3953456E-427.941334
GTCCACG700.00837414427.1476841
CCTAACC700.00838119127.1430053
CGACGAA350.00839503827.13832788-89
TATATTG908.93108E-426.3890345
TATCAAC21550.026.0094242
TCAACGC21550.026.0094244
CAACGCA21650.025.6698865
ATCAACG22100.025.3621313
TACATGG9400.025.2660962
AACGCAG21950.025.1026426
TAGACAG950.001224306325.0001355
GTTACAC1151.2784806E-424.7827433
GTACATG9900.024.4740491
GGTATCA12400.024.1373561
Produced by FastQC (version 0.11.5)