##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937304_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 290794 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.983307771136957 33.0 31.0 34.0 31.0 34.0 2 32.098255122182714 33.0 31.0 34.0 30.0 34.0 3 32.150814666052256 34.0 31.0 34.0 30.0 34.0 4 35.73373590926911 37.0 35.0 37.0 35.0 37.0 5 35.72890087140725 37.0 35.0 37.0 35.0 37.0 6 35.77176626752959 37.0 35.0 37.0 35.0 37.0 7 35.628678721019 37.0 35.0 37.0 33.0 37.0 8 35.66028528786701 37.0 35.0 37.0 33.0 37.0 9 37.33758261862349 39.0 37.0 39.0 35.0 39.0 10-11 37.24417285088413 39.0 37.0 39.0 34.0 39.0 12-13 37.11778097209709 39.0 37.0 39.0 34.0 39.0 14-15 38.36113021589166 40.0 38.0 41.0 34.0 41.0 16-17 38.295637117684684 40.0 38.0 41.0 34.0 41.0 18-19 38.246100332193926 40.0 38.0 41.0 34.0 41.0 20-21 38.13074719560926 40.0 38.0 41.0 34.0 41.0 22-23 37.970198834914065 40.0 38.0 41.0 33.5 41.0 24-25 37.903079155690975 40.0 38.0 41.0 33.0 41.0 26-27 37.893281842128786 40.0 38.0 41.0 33.0 41.0 28-29 37.80658473008384 40.0 38.0 41.0 33.0 41.0 30-31 37.59333411280838 40.0 37.0 41.0 33.0 41.0 32-33 37.34874515980385 40.0 37.0 41.0 32.5 41.0 34-35 37.42981457664189 40.0 37.0 41.0 33.0 41.0 36-37 37.34646003700214 40.0 37.0 41.0 32.5 41.0 38-39 37.22598299827369 40.0 37.0 41.0 32.0 41.0 40-41 37.00956347104823 40.0 36.5 41.0 31.5 41.0 42-43 36.74592323087822 40.0 36.0 41.0 31.0 41.0 44-45 36.504049258237785 39.0 36.0 41.0 30.0 41.0 46-47 36.12570582611745 39.0 35.5 41.0 29.5 41.0 48-49 35.75854384891022 39.0 35.0 41.0 28.0 41.0 50-51 34.761081727958626 38.0 34.0 39.5 26.0 40.5 52-53 34.85581545699017 38.0 34.5 39.5 26.5 40.5 54-55 35.19919427498505 39.0 35.0 40.0 26.5 41.0 56-57 34.95364244104074 38.5 35.0 40.0 26.0 41.0 58-59 34.66616573932062 38.0 34.0 40.0 26.0 41.0 60-61 34.31850725943451 38.0 34.0 40.0 24.5 41.0 62-63 33.968018597357585 37.5 34.0 40.0 23.0 41.0 64-65 33.50083392367105 37.0 33.0 40.0 22.0 41.0 66-67 33.13819060915975 36.0 33.0 39.0 21.0 41.0 68-69 32.63295494404974 36.0 32.5 39.0 18.5 40.5 70-71 32.12084671623211 35.0 32.0 38.5 16.5 40.0 72-73 31.652429211056624 35.0 31.0 37.5 11.5 40.0 74-75 31.179819734932636 35.0 31.0 37.0 7.5 39.0 76-77 30.70207947894386 35.0 31.0 36.5 4.5 39.0 78-79 30.20246291188952 34.0 30.0 36.0 2.0 38.5 80-81 29.73638211242323 34.0 30.0 35.5 2.0 37.5 82-83 29.274666946360654 34.0 29.0 35.0 2.0 37.0 84-85 28.767029237191963 34.0 29.0 35.0 2.0 36.5 86-87 28.3320494920803 34.0 28.5 35.0 2.0 36.0 88-89 27.89122884241078 34.0 27.0 35.0 2.0 35.5 90-91 27.395352036149305 33.0 26.5 35.0 2.0 35.0 92-93 26.88503545465175 33.0 25.0 35.0 2.0 35.0 94-95 26.363135071562688 33.0 25.0 35.0 2.0 35.0 96-97 25.665299146474823 32.5 22.0 35.0 2.0 35.0 98-99 24.727049045028437 32.0 10.0 34.0 2.0 35.0 100-101 22.20977736817128 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.08492333531018659 1101 2 -0.30518138403425255 1101 3 -0.2967030677924285 1101 4 -0.29009723279888533 1101 5 -0.5431073892553826 1101 6 -0.3116425587981908 1101 7 -0.4441185456838639 1101 8 -0.4962713440946658 1101 9 -0.3688668171343892 1101 10-11 -0.530021004465631 1101 12-13 -0.7396356508862354 1101 14-15 -0.8205721087345736 1101 16-17 -0.774087109467807 1101 18-19 -0.6184948998420268 1101 20-21 -0.6747338063243618 1101 22-23 -0.7168238884351226 1101 24-25 -0.6084921119800839 1101 26-27 -0.6002525127953007 1101 28-29 -0.9171626148316463 1101 30-31 -0.6030335249042622 1101 32-33 -0.6454256610159561 1101 34-35 -0.6796958743761081 1101 36-37 -0.516349815102231 1101 38-39 -0.728854875912667 1101 40-41 -0.5935641829884872 1101 42-43 -0.43667378132996504 1101 44-45 -0.5028238048798812 1101 46-47 -0.1544869418258159 1101 48-49 -0.43722567090529196 1101 50-51 -0.6841201459068742 1101 52-53 -0.7256345984567503 1101 54-55 -0.510828114597345 1101 56-57 -0.5406277265132431 1101 58-59 -0.820836502276066 1101 60-61 -0.7807392707498266 1101 62-63 -0.9011336676912336 1101 64-65 -0.8507406396662205 1101 66-67 -0.9611198970641652 1101 68-69 -0.8843114782168442 1101 70-71 -1.1199092677914066 1101 72-73 -0.9667322171471966 1101 74-75 -1.2446532194155147 1101 76-77 -0.6330399811587064 1101 78-79 -0.8106445219845888 1101 80-81 -0.7406210339098287 1101 82-83 -0.6982968694925304 1101 84-85 -0.9493158038992213 1101 86-87 -1.080680640605486 1101 88-89 -1.2552226396664352 1101 90-91 -1.164011708560249 1101 92-93 -1.0419576970847295 1101 94-95 -1.3729395246426783 1101 96-97 -0.9015013620506096 1101 98-99 -1.2731942117559676 1101 100-101 -1.3831930299411006 1102 1 -0.276861990830497 1102 2 -0.41573089539306096 1102 3 -0.28319008841516435 1102 4 -0.41509846798895467 1102 5 -0.5243374409357386 1102 6 -0.34558064119030973 1102 7 -0.13988629042442824 1102 8 -0.3132952277298813 1102 9 -0.4814914478125587 1102 10-11 -0.3663805534730358 1102 12-13 -0.34446870259623097 1102 14-15 -0.29242212701539216 1102 16-17 -0.4894869651976066 1102 18-19 -0.17849796311340071 1102 20-21 -0.3470971695986904 1102 22-23 -0.3783743978275069 1102 24-25 -0.23322160535452952 1102 26-27 -0.3456106685083 1102 28-29 -0.1740574458082378 1102 30-31 -0.05571339328240299 1102 32-33 -0.04289994435867328 1102 34-35 0.09957813030773366 1102 36-37 0.054821836791568046 1102 38-39 0.22374594030092965 1102 40-41 0.1735135549338196 1102 42-43 0.42106443742714106 1102 44-45 0.45005863466991514 1102 46-47 0.4121836774490788 1102 48-49 0.21964099773767742 1102 50-51 0.40802009263303063 1102 52-53 0.5916091490111128 1102 54-55 0.5782809675082063 1102 56-57 0.4607136899039048 1102 58-59 0.3639701657740133 1102 60-61 0.47578253282529914 1102 62-63 0.508820976129357 1102 64-65 0.7588651864783102 1102 66-67 0.5865979657605678 1102 68-69 0.42378445158482947 1102 70-71 0.5573355666437187 1102 72-73 0.5824678737627949 1102 74-75 0.2650874894847668 1102 76-77 0.002067579192683411 1102 78-79 0.014074595793253764 1102 80-81 -0.03225435044457825 1102 82-83 0.01701652293229472 1102 84-85 -0.5658732797636041 1102 86-87 -0.5610386480956819 1102 88-89 -0.1766521992470622 1102 90-91 -0.3856838594708272 1102 92-93 -0.45791536465061355 1102 94-95 -0.6801856436754754 1102 96-97 -0.6714175667561904 1102 98-99 -0.5411884607109982 1102 100-101 -0.2783748005119584 1103 1 -0.021909145713717493 1103 2 -0.05906275183966869 1103 3 -0.32623078719356613 1103 4 -0.15748722677115978 1103 5 -0.26584006768426605 1103 6 -0.1843679062070862 1103 7 -0.45996984001617136 1103 8 0.033435578017318335 1103 9 -0.3224940429135188 1103 10-11 -0.06826041928732707 1103 12-13 -0.07169827408809937 1103 14-15 -0.18118090921679197 1103 16-17 -0.33650259909848756 1103 18-19 -0.5824792277504116 1103 20-21 -0.5394194684783713 1103 22-23 -0.4852166030303735 1103 24-25 -0.5709328529018336 1103 26-27 -0.6629408792811304 1103 28-29 -0.5568348367823504 1103 30-31 -1.0091598139419418 1103 32-33 -0.4820416621889265 1103 34-35 -0.4451398898201262 1103 36-37 -0.3562484841148219 1103 38-39 -0.9553793207915433 1103 40-41 -0.8548743177163445 1103 42-43 -0.4386735206714789 1103 44-45 -0.4219585816095943 1103 46-47 -0.45271283498841797 1103 48-49 -0.41014569762278796 1103 50-51 -0.27518770532886094 1103 52-53 -0.4940924776741227 1103 54-55 -0.4269408819759377 1103 56-57 -0.5174494849805811 1103 58-59 -0.4996604437582377 1103 60-61 -0.7231541087342848 1103 62-63 -0.3479104625692955 1103 64-65 -0.20173358559588195 1103 66-67 0.13213882277683098 1103 68-69 -0.009280932300228528 1103 70-71 0.06578546853624445 1103 72-73 -0.049882274980799224 1103 74-75 -0.07955457646876951 1103 76-77 -0.23342200875591956 1103 78-79 -0.5653608115110096 1103 80-81 -0.6865995621668368 1103 82-83 -0.5433353980753601 1103 84-85 -0.5703672350772351 1103 86-87 -0.24557564410808652 1103 88-89 -0.4975902332998494 1103 90-91 -0.5615810681549256 1103 92-93 -0.2649917618909434 1103 94-95 -1.2925345265161639 1103 96-97 -1.1485363717438446 1103 98-99 -0.9154526546473214 1103 100-101 -0.47139334598951876 1104 1 -0.12447627118160653 1104 2 -0.22709010307312738 1104 3 -0.19411666652982618 1104 4 -0.3014348821691968 1104 5 -0.20656923742107125 1104 6 -0.12642337498010647 1104 7 -0.16091888065987092 1104 8 -0.2761373536930165 1104 9 -0.2845913637894597 1104 10-11 -0.098610562232885 1104 12-13 -0.15846033604099574 1104 14-15 0.11025856733719053 1104 16-17 -0.24492165064730642 1104 18-19 -0.4150855527231414 1104 20-21 -0.2127004375608692 1104 22-23 -0.3046262525926906 1104 24-25 -0.13318048766855384 1104 26-27 -0.25863335474460314 1104 28-29 -0.2191885754719678 1104 30-31 -0.34165561399499467 1104 32-33 -0.5026582438742011 1104 34-35 -0.17778747742954693 1104 36-37 -0.07305416656936359 1104 38-39 -0.05553700301029352 1104 40-41 0.22184471320116472 1104 42-43 -0.12459854056596242 1104 44-45 0.19250102521642276 1104 46-47 0.14044493565027238 1104 48-49 0.2517217314202185 1104 50-51 0.32443945727539614 1104 52-53 0.3433253654689139 1104 54-55 0.36216283460869647 1104 56-57 0.5411434591085538 1104 58-59 0.5623808778250705 1104 60-61 0.2728890991409827 1104 62-63 0.557509679492064 1104 64-65 0.2889749528225849 1104 66-67 0.3154849278786642 1104 68-69 -0.1693873309562406 1104 70-71 0.19773011765646586 1104 72-73 0.03146406590752804 1104 74-75 0.11443139423475657 1104 76-77 0.23836466341242968 1104 78-79 0.12567782800091365 1104 80-81 0.3549357026508737 1104 82-83 0.677375498208054 1104 84-85 0.9366474131587879 1104 86-87 0.8632914017818614 1104 88-89 0.6692950270968616 1104 90-91 0.6112725379047887 1104 92-93 0.9518581305314662 1104 94-95 1.088393293342957 1104 96-97 0.3723012597808051 1104 98-99 0.3862656394373829 1104 100-101 0.8690455116249147 1105 1 -0.08082059172193823 1105 2 0.048422613710208395 1105 3 -0.20915518624699914 1105 4 -0.07914621294627011 1105 5 -0.14328433222530634 1105 6 -0.19607546630174255 1105 7 -0.3075224617623462 1105 8 -0.2821703504918318 1105 9 -0.23506098773852813 1105 10-11 -0.2073188325392863 1105 12-13 8.512339452764195E-4 1105 14-15 -0.5452362087559592 1105 16-17 -0.07806443743452007 1105 18-19 -0.7184239282112372 1105 20-21 -0.33161027679821586 1105 22-23 -0.010126305359428045 1105 24-25 -0.15008343205241204 1105 26-27 -0.16294087928113044 1105 28-29 -0.22267118003805564 1105 30-31 0.028142465295822205 1105 32-33 0.062297607956800505 1105 34-35 -0.5011244458046775 1105 36-37 -0.3044363032703856 1105 38-39 -0.33842826835661555 1105 40-41 -0.5318577527642958 1105 42-43 -0.5931759493674562 1105 44-45 -0.39816981588534617 1105 46-47 -0.27161929905617654 1105 48-49 -0.49645781619297225 1105 50-51 -0.2518348593953661 1105 52-53 0.12503817371460002 1105 54-55 -0.028011999176086988 1105 56-57 0.00826978267417644 1105 58-59 -0.013796699829974557 1105 60-61 -0.01571856258906479 1105 62-63 -0.048744937597319904 1105 64-65 -0.08615256678905325 1105 66-67 0.053735534696123466 1105 68-69 -0.1670216222989822 1105 70-71 -0.48708537465718393 1105 72-73 0.03593164954280326 1105 74-75 0.053899153759150664 1105 76-77 0.41628530224171456 1105 78-79 0.5050090975685784 1105 80-81 0.5030255468131095 1105 82-83 0.633772585484234 1105 84-85 0.08295198275811444 1105 86-87 0.7160634143051681 1105 88-89 0.5179160563999865 1105 90-91 0.8161745676652288 1105 92-93 1.18475291366018 1105 94-95 1.1925196520864034 1105 96-97 1.1236644004569243 1105 98-99 1.0813090840498987 1105 100-101 1.1266263326753219 1106 1 -0.703195009320023 1106 2 -0.3927516103904054 1106 3 -0.6685589085272134 1106 4 -0.27587712416657695 1106 5 -0.30867374587705854 1106 6 -0.6634736891802717 1106 7 -0.9745285976688507 1106 8 -1.039432870541738 1106 9 -1.279361723538102 1106 10-11 -1.3680108405847022 1106 12-13 -0.538421113739112 1106 14-15 -0.6413229770936653 1106 16-17 -0.8384331010289898 1106 18-19 -0.9348864889974493 1106 20-21 -1.0704141569316263 1106 22-23 -0.7900865874583687 1106 24-25 -0.9298782228969316 1106 26-27 -1.3018516922250924 1106 28-29 -1.2836101519554646 1106 30-31 -0.650257106972937 1106 32-33 -0.8442507536797805 1106 34-35 -0.13411576387886726 1106 36-37 -1.116578269306487 1106 38-39 -0.8461299767355683 1106 40-41 -0.6924988404237382 1106 42-43 -0.8170092360237646 1106 44-45 -0.9313444473490549 1106 46-47 -0.7985179929482698 1106 48-49 -0.3252889387328821 1106 50-51 -0.8092221343909145 1106 52-53 -0.20122146933350393 1106 54-55 -0.517407828796479 1106 56-57 -0.46294746915258855 1106 58-59 -0.40919349693769647 1106 60-61 -0.7510343962830817 1106 62-63 -0.2911259748455848 1106 64-65 -0.32718421380894114 1106 66-67 -1.0167764697848654 1106 68-69 -0.6466025475223915 1106 70-71 -0.292263977910995 1106 72-73 -0.7246406955955749 1106 74-75 -0.15376691034961354 1106 76-77 0.24552378786943407 1106 78-79 -0.0010249681696414825 1106 80-81 -0.38239085464320866 1106 82-83 -0.1343996272611392 1106 84-85 -0.5083991896837752 1106 86-87 -0.7737652385960203 1106 88-89 -0.25075263010865356 1106 90-91 -0.4150549050541734 1106 92-93 -0.39030373526921736 1106 94-95 0.5948670818799222 1106 96-97 0.4456849213724752 1106 98-99 0.20440911714815257 1106 100-101 0.8320900900906025 1107 1 -0.0320696821900448 1107 2 -0.22467949640378393 1107 3 -0.15668530185860874 1107 4 0.01962221349617721 1107 5 -0.04273726563409497 1107 6 0.05155975077320818 1107 7 -0.032911202431222364 1107 8 0.10266269987602072 1107 9 0.09293642514852962 1107 10-11 0.13496159972416422 1107 12-13 0.03821615161580638 1107 14-15 0.043129867725561155 1107 16-17 0.12063122645638913 1107 18-19 0.06873213398726818 1107 20-21 0.4794226819427294 1107 22-23 0.14306581472573754 1107 24-25 -0.021846172236209327 1107 26-27 0.12903907059355646 1107 28-29 0.3666111656110189 1107 30-31 0.5113531802552629 1107 32-33 0.0186131279395525 1107 34-35 0.4738128975537137 1107 36-37 0.3058798095924189 1107 38-39 0.6571655549112307 1107 40-41 0.4446695870907149 1107 42-43 0.98761743193527 1107 44-45 0.5420166718393418 1107 46-47 0.8906681399715026 1107 48-49 0.49235831014438247 1107 50-51 0.337865197197722 1107 52-53 0.1082354141274493 1107 54-55 -0.03819875639170789 1107 56-57 0.33066178418332726 1107 58-59 0.45662667042360994 1107 60-61 0.9780696714368631 1107 62-63 0.6932848680997168 1107 64-65 -0.06581947599755722 1107 66-67 -0.1100206299089379 1107 68-69 0.5665731172380362 1107 70-71 0.021700029713958457 1107 72-73 0.142084127511918 1107 74-75 0.25369973046974437 1107 76-77 0.15933917347894422 1107 78-79 0.08359265323192844 1107 80-81 0.44958549507085266 1107 82-83 0.059568257459869045 1107 84-85 0.5188535646698789 1107 86-87 0.6393393460700629 1107 88-89 0.31389794660938364 1107 90-91 0.25944672655708345 1107 92-93 0.02118539270725961 1107 94-95 0.5979687587239653 1107 96-97 0.028008594274801624 1107 98-99 0.01940701705445491 1107 100-101 0.29535460496895993 1108 1 0.08153750651342406 1108 2 -0.03019939370801694 1108 3 0.23206095402500182 1108 4 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74-75 0.2627307468292095 2316 76-77 -0.05603576174942049 2316 78-79 0.17353269320528142 2316 80-81 -0.211085168930925 2316 82-83 -0.38606058257269993 2316 84-85 0.25532554631174875 2316 86-87 0.12290585728480963 2316 88-89 0.009545292823016638 2316 90-91 0.5427393462920733 2316 92-93 0.40108027759881537 2316 94-95 0.8127144837961424 2316 96-97 1.2303551780337791 2316 98-99 0.7236412312820093 2316 100-101 0.6590010628859027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1187.0 3 263.0 4 186.0 5 202.0 6 233.0 7 278.0 8 323.0 9 384.0 10 505.0 11 700.0 12 887.0 13 1146.0 14 1333.0 15 1656.0 16 2225.0 17 3113.0 18 2844.0 19 1890.0 20 1814.0 21 2001.0 22 2264.0 23 2446.0 24 2626.0 25 3001.0 26 3292.0 27 3880.0 28 4449.0 29 5323.0 30 6737.0 31 8341.0 32 10627.0 33 14396.0 34 19855.0 35 29135.0 36 43432.0 37 58785.0 38 43797.0 39 5238.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.249724366041896 18.364457001102537 14.871830209481809 23.51398842337376 2 15.250342770133871 19.39975609924143 40.42827319640901 24.921627934215692 3 19.02626212522046 23.37790729717813 30.460827270723108 27.135003306878307 4 12.590084193410592 15.66569290763528 37.31724793650356 34.426974962450565 5 13.569236174984239 36.09644182177072 35.37440275036257 14.959919252882473 6 32.343466291805775 35.09531733972802 17.54748639923374 15.013729969232465 7 27.90470120070975 32.10701304070699 21.53146479698186 18.456820961601405 8 26.053642486778866 33.99493548776076 20.39758143701228 19.553840588448086 9 25.47284215168156 16.069742925454584 20.891182570469844 37.56623235239401 10-11 24.684428594809244 25.521358244825183 28.078834707255517 21.71537845311006 12-13 25.4748561002965 23.131974247078503 28.665372244111786 22.72779740851321 14-15 22.5085182543866 24.444203280518433 26.86376649819644 26.183511966898525 16-17 21.847759425023604 27.74205996458079 27.578401174208757 22.831779436186853 18-19 21.740418619633537 26.320911861119296 29.999293745166028 21.93937577408114 20-21 22.4070392789838 25.19215729508677 30.050265798929914 22.350537626999518 22-23 22.201647437377055 25.036431723677005 30.27450399457055 22.487416844375392 24-25 22.168883468890705 25.266205737159943 30.112510894540844 22.45239989940851 26-27 21.68295939020714 25.486757676241332 30.30033159639981 22.529951337151715 28-29 21.714654838292923 25.88708496619439 30.080186038499633 22.318074157013047 30-31 21.70025184577805 25.692575889642626 29.814784828617398 22.79238743596192 32-33 21.69718204321246 25.63326081301989 29.94658301853316 22.722974125234487 34-35 22.1536028609967 25.60960694025981 29.721428362248208 22.51536183649528 36-37 21.96484797541701 25.46024210939707 29.970855926306143 22.60405398887978 38-39 21.743916051152024 25.89106079814794 29.644436941902768 22.720586208797265 40-41 21.708716524758803 25.999541806694097 29.79651738636892 22.495224282178174 42-43 21.677133968835804 26.063754655164693 29.256310525753698 23.002800850245805 44-45 21.83971803714716 25.896896134724773 29.248994835504444 23.014390992623625 46-47 22.157007993384788 26.302542723263507 28.925888919514886 22.614560363836826 48-49 22.145809179097665 26.340161696277676 28.58058369841043 22.93344542621423 50-51 21.76180263055893 26.48853975554924 28.8979043386134 22.85175327527843 52-53 22.106572615451945 26.540464822973398 28.293931870301677 23.059030691272984 54-55 21.897524772943395 26.572341129287892 28.326603178103337 23.203530919665376 56-57 21.952605949319338 26.486856134923354 28.378552960448687 23.181984955308618 58-59 22.234891676168758 26.189254083028175 28.44254134701175 23.133312893791317 60-61 21.773721269911263 26.12491732348564 29.05597200022047 23.045389406382625 62-63 21.621156088679744 26.062377596841674 29.255495977166913 23.06097033731167 64-65 21.636601955627206 26.115046823785136 29.129096542945774 23.11925467764188 66-67 21.2401437148851 25.959965139150594 29.014423159729517 23.78546798623479 68-69 21.483812515932563 25.779748248279283 29.28181173050027 23.45462750528789 70-71 21.6758077074132 25.79048183390407 28.926226288990097 23.607484169692626 72-73 21.303023726104225 25.430718190931923 29.176183635292535 24.090074447671313 74-75 21.159487190970204 25.807185044964665 29.143923095870072 23.88940466819506 76-77 21.40572383322855 25.504388042476595 29.15813316567767 23.931754958617184 78-79 21.0387699982086 25.19705383841585 29.159489589218534 24.604686574157007 80-81 21.34862619191975 25.119536460342832 29.23665463264069 24.29518271509673 82-83 21.15988810726269 25.20170319486837 29.51205396206395 24.126354735804988 84-85 20.839979467321694 25.166483503233206 28.97932607340088 25.01421095604422 86-87 20.86452630889917 25.146677323668538 29.106534418785724 24.882261948646573 88-89 20.957715620365537 24.4151857918697 29.091977008762093 25.535121579002666 90-91 20.586173337122876 23.803150487903608 30.10843259351127 25.502243581462245 92-93 20.463106981743394 23.7678643096199 30.00807844880003 25.760950259836672 94-95 19.93371905353289 24.023914882036106 30.306824973861403 25.73554109056961 96-97 19.47214844059517 24.13882199737487 31.067864168311683 25.32116539371828 98-99 19.992352040403276 25.716091892646077 29.385045482966927 24.906510583983728 100-101 19.898242145113453 25.76809267749925 28.220925465988284 26.112739711399012 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 337.0 1 200.0 2 58.5 3 47.0 4 41.5 5 49.0 6 73.0 7 131.5 8 235.5 9 314.0 10 285.5 11 268.0 12 325.0 13 377.0 14 415.5 15 434.0 16 455.0 17 519.0 18 582.0 19 603.0 20 638.5 21 739.5 22 844.5 23 988.0 24 1151.5 25 1344.5 26 1706.0 27 2063.5 28 2371.0 29 2743.0 30 3030.0 31 3518.5 32 4160.0 33 4474.0 34 4700.5 35 5124.5 36 5656.5 37 6235.0 38 6879.5 39 7366.0 40 7782.5 41 8403.0 42 8857.0 43 9277.0 44 9869.0 45 10335.0 46 10940.0 47 11905.5 48 13081.5 49 13124.0 50 12031.5 51 11445.5 52 11201.5 53 10978.0 54 10707.5 55 9944.0 56 9026.0 57 8044.5 58 7072.5 59 6080.0 60 4858.5 61 3870.0 62 3037.5 63 2195.5 64 1551.5 65 1101.0 66 759.0 67 548.5 68 412.0 69 295.0 70 207.0 71 151.0 72 106.5 73 77.0 74 54.0 75 38.5 76 30.5 77 21.5 78 16.5 79 11.0 80 6.0 81 4.5 82 5.0 83 3.0 84 1.0 85 1.0 86 1.5 87 2.0 88 2.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19051287165484845 2 0.1760696575582715 3 0.16850416446006453 4 0.1753818854584345 5 0.173662455208842 6 0.19016898560492995 7 0.18879344140525595 8 0.18535458090607096 9 0.18157183435696747 10-11 0.19481144727882968 12-13 0.1975625356781777 14-15 0.18397903670639698 16-17 0.19120064375468543 18-19 0.1824315494817637 20-21 0.18466680880623396 22-23 0.18054017620721197 24-25 0.17572577150835297 26-27 0.1827754355316822 28-29 0.1827754355316822 30-31 0.18466680880623396 32-33 0.1824315494817637 34-35 0.1875898402305412 36-37 0.1760696575582715 38-39 0.17950851805745646 40-41 0.18002434713233423 42-43 0.18054017620721197 44-45 0.18673012510574497 46-47 0.19051287165484845 48-49 0.1920603588794817 50-51 0.1896531565300522 52-53 0.20547191482630317 54-55 0.19188841585452246 56-57 0.18071211923217123 58-59 0.174350227308679 60-61 0.1740063412587605 62-63 0.17658548663314924 64-65 0.17314662613396425 66-67 0.170911366809494 68-69 0.1746941133585975 70-71 0.18157183435696747 72-73 0.179852404107375 74-75 0.17555382848339376 76-77 0.17761714478290472 78-79 0.17813297385778248 80-81 0.1740063412587605 82-83 0.1774452017579455 84-85 0.18054017620721197 86-87 0.18260349250672298 88-89 0.17520994243347524 90-91 0.17933657503249725 92-93 0.17727325873298624 94-95 0.17692937268306774 96-97 0.179852404107375 98-99 0.17899268898257872 100-101 0.170911366809494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 290794.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.8425019723736 #Duplication Level Percentage of deduplicated Percentage of total 1 86.14009717572269 64.46940392774478 2 8.877749884582167 13.288660264941607 3 2.3486349029534814 5.273331370700442 4 0.9803762647524894 2.9349525011362574 5 0.5203758781763804 1.9473116344395696 6 0.29246009812806323 1.3133067282594089 7 0.19479519412211632 1.0205271790205372 8 0.12998266390828367 0.7782582223944076 9 0.08940164679044654 0.60219386336227 >10 0.3948791502717014 4.911937061071707 >50 0.022056805123931628 1.1947567156179844 >100 0.007811785148057778 1.274544728865775 >500 4.595167734151634E-4 0.20462874087294552 >1k 9.190335468303268E-4 0.7861870615723588 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1266 0.43535973919681975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1020 0.350763770916869 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 595 0.2046121997015069 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 474 0.16300198766136853 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 405 0.1392738502169921 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 360 0.12379897797065965 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 345 0.11864068722188216 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 319 0.10969964992400118 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.7194302495924951E-4 0.0 24-25 0.0 0.0 0.0 6.877720998369981E-4 0.0 26-27 0.0 0.0 0.0 0.0017194302495924952 0.0 28-29 0.0 0.0 0.0 0.004986347723818236 0.0 30-31 0.0 0.0 0.0 0.0173662455208842 0.0 32-33 0.0 0.0 0.0 0.03215334566737965 0.0 34-35 0.0 0.0 0.0 0.04418935741452712 0.0 36-37 0.0 0.0 0.0 0.06155560293541132 0.0 38-39 0.0 0.0 0.0 0.08390819618011375 0.0 40-41 0.0 0.0 0.0 0.10179027077587571 0.0 42-43 0.0 0.0 0.0 0.15165374801405807 0.0 44-45 0.0 0.0 0.0 0.20581580087622164 0.0 46-47 0.0 0.0 0.0 0.27373329573512517 0.0 48-49 0.0 0.0 0.0 0.35282708721638 0.0 50-51 0.0 0.0 0.0 0.44963101026843744 0.0 52-53 0.0 0.0 0.0 0.6403158249482452 0.0 54-55 0.0 0.0 0.0 0.795924262536366 0.0 56-57 0.0 0.0 0.0 1.123303782058777 0.0 58-59 0.0 0.0 0.0 1.521007998789521 0.0 60-61 0.0 0.0 0.0 1.91527335502108 0.0 62-63 0.0 0.0 0.0 2.5368473902487674 0.0 64-65 0.0 0.0 0.0 2.9348954930294298 0.0 66-67 0.0 0.0 0.0 3.620604276566917 0.0 68-69 0.0 0.0 0.0 4.342077209295928 0.0 70-71 0.0 0.0 0.0 5.019016898560493 0.0 72-73 0.0 0.0 0.0 6.127533580472774 0.0 74-75 3.4388604991849903E-4 0.0 0.0 7.176901861799074 0.0 76-77 3.4388604991849903E-4 0.0 0.0 7.980219674408688 0.0 78-79 3.4388604991849903E-4 0.0 0.0 8.85712910170086 0.0 80-81 3.4388604991849903E-4 0.0 0.0 10.308672118406845 0.0 82-83 3.4388604991849903E-4 0.0 0.0 11.2215176379155 0.0 84-85 3.4388604991849903E-4 0.0 0.0 12.539804810278067 0.0 86-87 3.4388604991849903E-4 0.0 0.0 14.701472520065751 0.0 88-89 3.4388604991849903E-4 0.0 0.0 17.394100290927597 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCCA 55 0.0025763917 34.545643 9 GTATCAA 1805 0.0 31.32136 1 GTATTAC 80 4.480783E-4 29.69278 1 AACCGCT 65 0.0058368123 29.230928 7 CTATAAG 85 6.3889E-4 27.946146 1 TAGGGGA 85 6.3953456E-4 27.94133 4 GTCCACG 70 0.008374144 27.147684 1 CCTAACC 70 0.008381191 27.143005 3 CGACGAA 35 0.008395038 27.138327 88-89 TATATTG 90 8.93108E-4 26.389034 5 TATCAAC 2155 0.0 26.009424 2 TCAACGC 2155 0.0 26.009424 4 CAACGCA 2165 0.0 25.669886 5 ATCAACG 2210 0.0 25.362131 3 TACATGG 940 0.0 25.266096 2 AACGCAG 2195 0.0 25.102642 6 TAGACAG 95 0.0012243063 25.000135 5 GTTACAC 115 1.2784806E-4 24.782743 3 GTACATG 990 0.0 24.474049 1 GGTATCA 1240 0.0 24.137356 1 >>END_MODULE