FastQCFastQC Report
Thu 26 May 2016
SRR937304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290794
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.4714677744382621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11810.4061294249537473No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9820.337696101019966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.2592900816385483No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC3330.11451405462286016No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3080.10591690337489769No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG156.615292E-494.9913945
GCCGCGC651.3264513E-436.5351568
GCTCCGT600.003947890431.66386
GGGCGTA650.0058395729.2281259
TCATACC350.00839192427.14040268-69
AGGGCGT950.001224997624.9977368
TACATGG10950.023.8562872
TCCTAGA1652.4214241E-623.028222
GTACATG11250.022.8175541
GAGTACT12700.022.81289512-13
TCCTACA1252.2516584E-422.7979352
GTGTAGG1050.00218251622.6364651
CGTCTGC1050.002193458822.6170028
TGCATAG1050.002193458822.6170025
GTAGATC1050.002193458822.6170023
TATACTG1050.002193458822.6170025
AGAGTAC18350.022.51839610-11
AGTACTT13350.022.23581512-13
ACTGGTC1503.0252837E-522.164668
CCTAGAC1302.9359947E-421.9210933