##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937304_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 290794 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42071363233079 33.0 31.0 34.0 31.0 34.0 2 32.62221022442004 34.0 31.0 34.0 31.0 34.0 3 32.68278919097368 34.0 31.0 34.0 31.0 34.0 4 36.26683494157376 37.0 35.0 37.0 35.0 37.0 5 36.204041348858645 37.0 35.0 37.0 35.0 37.0 6 36.25310357160051 37.0 37.0 37.0 35.0 37.0 7 36.20232191860905 37.0 36.0 37.0 35.0 37.0 8 36.186826413199725 37.0 36.0 37.0 35.0 37.0 9 37.93342366073578 39.0 38.0 39.0 35.0 39.0 10-11 37.86749898553615 39.0 38.0 39.0 35.0 39.0 12-13 37.77524983321527 39.0 37.0 39.0 35.0 39.0 14-15 39.0341753956409 40.0 38.0 41.0 36.0 41.0 16-17 38.99279042896346 40.0 38.0 41.0 36.0 41.0 18-19 38.977274290391136 40.0 38.0 41.0 36.0 41.0 20-21 38.86853752140691 40.0 38.0 41.0 35.0 41.0 22-23 38.714149879295995 40.0 38.0 41.0 35.0 41.0 24-25 38.64573890795546 40.0 38.0 41.0 34.5 41.0 26-27 38.58150959098194 40.0 38.0 41.0 34.5 41.0 28-29 38.44820560259153 40.0 38.0 41.0 34.0 41.0 30-31 38.569332585954314 40.0 38.0 41.0 35.0 41.0 32-33 38.523145250589764 40.0 38.0 41.0 35.0 41.0 34-35 38.38639208511867 40.0 38.0 41.0 34.0 41.0 36-37 38.23926215809129 40.0 38.0 41.0 34.0 41.0 38-39 38.095818689519035 40.0 38.0 41.0 34.0 41.0 40-41 37.90978665309463 40.0 37.5 41.0 33.0 41.0 42-43 37.70864942192755 40.0 37.0 41.0 33.0 41.0 44-45 37.388230156055485 40.0 37.0 41.0 33.0 41.0 46-47 37.01126398756508 40.0 37.0 41.0 31.5 41.0 48-49 36.648589723309286 40.0 36.0 41.0 31.0 41.0 50-51 36.36150333225582 39.0 36.0 41.0 30.5 41.0 52-53 36.10266718020317 39.0 35.0 41.0 30.0 41.0 54-55 35.8601535795099 39.0 35.0 40.5 29.0 41.0 56-57 35.54401397552907 38.5 35.0 40.0 28.5 41.0 58-59 35.23535045427347 38.0 35.0 40.0 28.0 41.0 60-61 34.84150807788332 38.0 34.0 40.0 27.0 41.0 62-63 34.43022895933204 37.0 34.0 40.0 26.0 41.0 64-65 34.02458957199942 37.0 34.0 39.5 26.0 41.0 66-67 33.595858236414784 36.0 33.0 39.0 25.5 40.5 68-69 33.132476942440356 35.5 33.0 39.0 24.5 40.0 70-71 32.69910486461206 35.0 32.5 38.0 23.5 40.0 72-73 32.22019711548381 35.0 32.0 37.0 21.5 39.5 74-75 31.75013583498972 35.0 31.5 37.0 20.0 39.0 76-77 30.514119961209655 34.0 30.0 35.5 19.5 38.0 78-79 31.095423220561635 34.0 31.0 36.0 19.5 38.0 80-81 30.92176592364354 34.0 31.0 35.5 18.0 37.0 82-83 30.60163724148366 34.0 31.0 35.0 17.0 37.0 84-85 30.28156014223127 34.0 31.0 35.0 12.0 36.5 86-87 29.9365100380338 34.0 31.0 35.0 7.0 36.0 88-89 29.53185072594345 34.0 30.0 35.0 3.5 35.5 90-91 29.094921146928755 34.0 29.5 35.0 2.0 35.0 92-93 28.77522576119177 34.0 29.0 35.0 2.0 35.0 94-95 28.3957887714327 34.0 29.0 35.0 2.0 35.0 96-97 27.981122375289722 34.0 29.0 35.0 2.0 35.0 98-99 27.28658087856008 33.5 27.0 35.0 2.0 35.0 100-101 24.90679312503009 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2204913781849953 1101 2 -0.042899891257967226 1101 3 -0.2432235457576013 1101 4 -0.22405723628521912 1101 5 -0.09372175478425504 1101 6 -0.2495489882630153 1101 7 -0.1509653670671014 1101 8 -0.15763261186724264 1101 9 -0.19593162624870075 1101 10-11 -0.0808020611757101 1101 12-13 -0.06459406419334357 1101 14-15 -0.2657668073246455 1101 16-17 -0.12446295556577525 1101 18-19 -0.11271205714070476 1101 20-21 -0.010348915492777166 1101 22-23 -0.12206324541590874 1101 24-25 -0.3065233652786148 1101 26-27 -0.4201541700812399 1101 28-29 -0.5444607705036049 1101 30-31 -0.388063946816807 1101 32-33 -0.32355424914280917 1101 34-35 -0.31123642761762227 1101 36-37 -0.17350646225290944 1101 38-39 -0.30931782130718233 1101 40-41 -0.31123976665239894 1101 42-43 -0.22844743571903336 1101 44-45 -0.17615261649479663 1101 46-47 -0.3538651568807296 1101 48-49 -0.45084328895352854 1101 50-51 -0.5586040712547273 1101 52-53 -0.4672014756708691 1101 54-55 -0.6625411468462801 1101 56-57 -0.6681286426408377 1101 58-59 -0.6802252972111162 1101 60-61 -0.5131908376464054 1101 62-63 -0.6878241475874916 1101 64-65 -0.8374597083273798 1101 66-67 -0.5376200851474735 1101 68-69 -0.42336556333876985 1101 70-71 -0.6607716222707651 1101 72-73 -0.5602637361537646 1101 74-75 -0.6279650579047029 1101 76-77 -0.6304284183708475 1101 78-79 -0.49856595400619597 1101 80-81 -0.4069844334234993 1101 82-83 -0.29098150060533357 1101 84-85 -0.42774136534192664 1101 86-87 -0.736003439014361 1101 88-89 -0.5369718518047009 1101 90-91 -0.8442116493144276 1101 92-93 -1.0896860444312217 1101 94-95 -0.8634653522990554 1101 96-97 -1.2187154136082476 1101 98-99 -1.2275206239053844 1101 100-101 -0.4963358675895435 1102 1 -0.1302434508339374 1102 2 -0.021175368288375296 1102 3 -0.17239280632341547 1102 4 0.07450796692825179 1102 5 0.02538515338677172 1102 6 -0.0924081074242693 1102 7 -0.2353337896799701 1102 8 -0.24805840658853384 1102 9 -0.5926203287062322 1102 10-11 -0.32309941589265634 1102 12-13 -0.4337701430083527 1102 14-15 -0.41954550079000086 1102 16-17 -0.4886488664937474 1102 18-19 -0.4225594864631219 1102 20-21 -0.4549629927069816 1102 22-23 -0.449373791962806 1102 24-25 -0.38164762539975783 1102 26-27 -0.3159535356876191 1102 28-29 -0.7268983439974122 1102 30-31 -0.771281666359485 1102 32-33 -0.6193081597545742 1102 34-35 -0.5048919973366921 1102 36-37 -0.8084979147376146 1102 38-39 -0.5557777665635584 1102 40-41 -0.38316488448953834 1102 42-43 -0.463346001910395 1102 44-45 -0.39566812554132724 1102 46-47 -0.31312611795740963 1102 48-49 -0.4620810482199218 1102 50-51 -0.6592522990040166 1102 52-53 -0.7061366430144176 1102 54-55 -0.5948601420932675 1102 56-57 -0.4779069013192867 1102 58-59 -0.6694247952298795 1102 60-61 -0.32021956322668643 1102 62-63 -0.5086030090386942 1102 64-65 -0.28425513120705403 1102 66-67 -0.47412637473532016 1102 68-69 -0.3310746316101927 1102 70-71 -0.4334398304546454 1102 72-73 -0.49991729004263874 1102 74-75 -0.5249600874998563 1102 76-77 -0.41828699404847214 1102 78-79 -0.2713502700567503 1102 80-81 -0.5252785865277794 1102 82-83 -0.6354513076192383 1102 84-85 -0.46111373068150385 1102 86-87 -0.4029887847193585 1102 88-89 -0.3984403445763718 1102 90-91 -0.42928165988765343 1102 92-93 -0.7753227602090433 1102 94-95 -0.6042038971463377 1102 96-97 -0.6185266765653914 1102 98-99 -0.44370111976008886 1102 100-101 -0.007220362495615973 1103 1 -0.18391773401641842 1103 2 -0.022821042065196195 1103 3 -0.16808873644557565 1103 4 -0.012459562662229473 1103 5 0.17742009230696 1103 6 0.15374671250103944 1103 7 0.059874767823664854 1103 8 -0.13311442279209018 1103 9 -0.038534627079549466 1103 10-11 -0.10919347247851618 1103 12-13 -0.17501958917582527 1103 14-15 -0.14025567231984581 1103 16-17 -0.30942866268338065 1103 18-19 -0.21026756925317613 1103 20-21 -0.13298058875736274 1103 22-23 -0.10618751435030305 1103 24-25 0.05334826041580243 1103 26-27 -0.052772321686731516 1103 28-29 -0.4423233708978458 1103 30-31 -0.3392922999438923 1103 32-33 -0.22425151059956505 1103 34-35 -0.3170320061980121 1103 36-37 -0.5490827618798448 1103 38-39 -0.17654490372414244 1103 40-41 -0.06861966010824716 1103 42-43 0.31559570326081143 1103 44-45 0.31874417903490837 1103 46-47 0.4309083146016093 1103 48-49 0.37186413818428576 1103 50-51 0.7532738102434067 1103 52-53 0.5544494700307112 1103 54-55 0.6462943613563255 1103 56-57 0.719352418890864 1103 58-59 0.7527854712426816 1103 60-61 0.14870252044948984 1103 62-63 0.30255592507106144 1103 64-65 0.0776348618304823 1103 66-67 0.4571667450823895 1103 68-69 0.51853610324644 1103 70-71 0.29246054298671 1103 72-73 0.2746254025328412 1103 74-75 0.40513042455786774 1103 76-77 0.4840169492390807 1103 78-79 0.15842376627466237 1103 80-81 -0.3090623703115618 1103 82-83 -0.2422302176768376 1103 84-85 -0.1912548788554389 1103 86-87 -0.20762831886709066 1103 88-89 -0.023796379768469933 1103 90-91 -0.23945951924584108 1103 92-93 -0.3297565851346711 1103 94-95 -0.6617075366171896 1103 96-97 -0.568396921517607 1103 98-99 -0.14643547003542068 1103 100-101 0.6441183298266822 1104 1 0.3214611454082643 1104 2 0.21100547802086567 1104 3 0.3227892735164062 1104 4 0.11070940783498884 1104 5 0.23801722160637695 1104 6 0.27505886434754245 1104 7 0.007117539493918912 1104 8 -0.015412751655304646 1104 9 0.17284796598243446 1104 10-11 0.21429086245620255 1104 12-13 0.20158302215960333 1104 14-15 0.13559242223053758 1104 16-17 0.15192445706668423 1104 18-19 0.12420294269825405 1104 20-21 0.22671907819421477 1104 22-23 0.13805045317010212 1104 24-25 0.4945121615033585 1104 26-27 -0.018038023593653918 1104 28-29 0.3274753263817658 1104 30-31 0.171104410834495 1104 32-33 0.2091461727981212 1104 34-35 0.37842763010132074 1104 36-37 0.29182030039726214 1104 38-39 0.2549959610228356 1104 40-41 0.2169030490785886 1104 42-43 0.37420338400590225 1104 44-45 0.4729117053398397 1104 46-47 0.7696008784850079 1104 48-49 0.4144899155428803 1104 50-51 0.44406929493202085 1104 52-53 0.5859195988290864 1104 54-55 0.6966642514208701 1104 56-57 0.7800305997980459 1104 58-59 0.33786018608839186 1104 60-61 0.5733392618801219 1104 62-63 0.7013519402792951 1104 64-65 0.726556138614356 1104 66-67 0.2724501309764591 1104 68-69 0.12296741569302583 1104 70-71 0.4462596528926923 1104 72-73 0.4721629073222502 1104 74-75 0.7840393225507398 1104 76-77 0.664511511413032 1104 78-79 -0.03360959751442749 1104 80-81 0.42373995218541793 1104 82-83 0.23177432121319796 1104 84-85 0.43867084839853376 1104 86-87 0.22946826517605956 1104 88-89 0.4494197480278075 1104 90-91 0.6088192393382599 1104 92-93 0.7650664276272714 1104 94-95 0.5870693209382942 1104 96-97 0.5839064178086346 1104 98-99 0.9740366924214747 1104 100-101 1.3310307056703543 1105 1 0.04313420271294177 1105 2 -0.012109870063703454 1105 3 0.01836038548741925 1105 4 -0.034504649223443096 1105 5 -0.12797285824728277 1105 6 -0.007419061567958352 1105 7 -0.10826572289747105 1105 8 0.09854580886813835 1105 9 0.07505361554095202 1105 10-11 -0.044864166213727685 1105 12-13 -0.026993683085407838 1105 14-15 -0.19237922444339262 1105 16-17 0.08806791223254606 1105 18-19 0.11997280937429622 1105 20-21 0.11412365834688387 1105 22-23 -0.10326062432663718 1105 24-25 -0.4682609063546579 1105 26-27 -0.05924884429229138 1105 28-29 -0.4313631018730675 1105 30-31 -0.0588090517187112 1105 32-33 -0.22288147697146599 1105 34-35 -0.3951239229996091 1105 36-37 -0.17780364866524678 1105 38-39 0.05667218433165999 1105 40-41 -0.19441365686997614 1105 42-43 -0.1451092413150974 1105 44-45 0.11604147633220663 1105 46-47 0.2750358522775329 1105 48-49 0.41402380937621075 1105 50-51 0.37153262205060145 1105 52-53 0.27135070306098186 1105 54-55 0.5552493993436229 1105 56-57 0.35959404300345454 1105 58-59 0.3746561898875953 1105 60-61 0.0654418404146142 1105 62-63 -0.21718501402471446 1105 64-65 -0.22532939274667285 1105 66-67 0.31219227752728074 1105 68-69 0.06667410299734655 1105 70-71 -0.23120367422588828 1105 72-73 -0.44918827289373553 1105 74-75 -0.03191030280543927 1105 76-77 -0.043881917369464674 1105 78-79 -0.049509973917139405 1105 80-81 -0.17504817178123488 1105 82-83 0.11653177011740823 1105 84-85 0.2969130125382584 1105 86-87 0.05840730200724309 1105 88-89 0.3667254784953222 1105 90-91 0.3665935384201191 1105 92-93 0.6549862221887786 1105 94-95 0.41657120295187156 1105 96-97 0.4217014718065961 1105 98-99 -0.08142114695658265 1105 100-101 0.4985211197133417 1106 1 -0.17088417954639823 1106 2 -0.09920919027654662 1106 3 -0.07892301192073603 1106 4 -0.10100667330878821 1106 5 -0.11422435403361675 1106 6 -0.03066421776624395 1106 7 -0.3579029151473847 1106 8 -0.22031764811686116 1106 9 -0.2039391792244345 1106 10-11 -0.23944369112652453 1106 12-13 -0.2132349947945542 1106 14-15 -0.3727265014757535 1106 16-17 -0.12842010874665277 1106 18-19 -0.350671588110238 1106 20-21 -0.19803103778571085 1106 22-23 -0.32303301020132125 1106 24-25 -0.3483561274764355 1106 26-27 -0.5119438057201009 1106 28-29 -0.5493329060841816 1106 30-31 -0.6228414370841904 1106 32-33 -0.5159919487488409 1106 34-35 -0.626264285043554 1106 36-37 -0.749257680286803 1106 38-39 -0.8652541338698327 1106 40-41 -1.140336206962914 1106 42-43 -1.0227220936205157 1106 44-45 -0.8093728883086797 1106 46-47 -0.57281403442461 1106 48-49 -0.7240850074886183 1106 50-51 -1.4163330499711932 1106 52-53 -0.5940285265135898 1106 54-55 -0.34884205366959975 1106 56-57 -0.8129114945885192 1106 58-59 -0.5291424709062511 1106 60-61 -0.1972946211277602 1106 62-63 -0.0764410671855984 1106 64-65 -0.13756810751613102 1106 66-67 -0.253940573759742 1106 68-69 -0.47254732430106827 1106 70-71 -0.7444429745797905 1106 72-73 -0.3724767114201022 1106 74-75 -0.4298276590189971 1106 76-77 -0.3061982857275396 1106 78-79 -0.03422112802768851 1106 80-81 -0.07618624240781102 1106 82-83 -0.1533844658476582 1106 84-85 -0.03685738744137623 1106 86-87 0.20120079438191496 1106 88-89 0.10853366140134924 1106 90-91 -0.17302252186961908 1106 92-93 -0.39845472289358597 1106 94-95 -0.25103324748986466 1106 96-97 -0.21475243251400045 1106 98-99 0.20629176390728432 1106 100-101 0.9576702400636705 1107 1 0.09870323281507609 1107 2 -0.18676694285320394 1107 3 -0.011526158830363897 1107 4 -0.19137532052535278 1107 5 0.007581080362683679 1107 6 -0.03528442389851705 1107 7 -0.04466586303275477 1107 8 0.058445558241572826 1107 9 -0.06316828329214985 1107 10-11 -0.10091827999279701 1107 12-13 0.03073127446977253 1107 14-15 -0.15186126287280644 1107 16-17 0.08441631010896344 1107 18-19 0.09080041602533839 1107 20-21 -0.33616873931393343 1107 22-23 0.0075540467596795224 1107 24-25 -0.16395174635788834 1107 26-27 -0.24153251044692325 1107 28-29 -0.40329562134377994 1107 30-31 -0.13014330132121188 1107 32-33 -0.5145380377281938 1107 34-35 -0.17671499745995334 1107 36-37 -0.18039076160889067 1107 38-39 -0.3424438854125924 1107 40-41 -0.1300457478501187 1107 42-43 -0.6010878976333061 1107 44-45 -0.3225550148958618 1107 46-47 -0.3963738547858213 1107 48-49 0.11790717370099912 1107 50-51 -0.028364971395376415 1107 52-53 -0.07142828742599505 1107 54-55 0.04234078371852945 1107 56-57 -0.2825893725246189 1107 58-59 0.2732871759549127 1107 60-61 0.12838144749683522 1107 62-63 0.03717400179439778 1107 64-65 -0.17095959729029175 1107 66-67 -0.30048188098729156 1107 68-69 -0.30557372086337864 1107 70-71 0.16576994261190237 1107 72-73 0.020386200925223363 1107 74-75 0.2011858785080598 1107 76-77 -0.09782052733523727 1107 78-79 -0.6124900046391097 1107 80-81 -0.5916946266280299 1107 82-83 -0.9335531195260529 1107 84-85 -0.9467472132951436 1107 86-87 -0.4321874644788686 1107 88-89 -0.5383598153845384 1107 90-91 -0.24709121108806187 1107 92-93 0.01870465806341315 1107 94-95 -0.3503999884675366 1107 96-97 -0.0771801408271422 1107 98-99 -0.10473211254258885 1107 100-101 0.42978745760107273 1108 1 0.1496588739504361 1108 2 0.08705276902812642 1108 3 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-0.2827524425220034 2316 90-91 0.05241282325941299 2316 92-93 0.8776135336278514 2316 94-95 0.967758276392928 2316 96-97 0.7833942910331011 2316 98-99 0.8241671398076953 2316 100-101 -1.0490784836232976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 0.0 5 6.0 6 18.0 7 16.0 8 70.0 9 110.0 10 187.0 11 308.0 12 515.0 13 759.0 14 1001.0 15 1376.0 16 2039.0 17 3167.0 18 2891.0 19 1755.0 20 1514.0 21 1541.0 22 1590.0 23 1609.0 24 1815.0 25 2069.0 26 2539.0 27 2991.0 28 3519.0 29 4536.0 30 5610.0 31 6996.0 32 9232.0 33 12754.0 34 18057.0 35 28223.0 36 45437.0 37 68374.0 38 53063.0 39 5106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92261296504646 19.26280694873092 14.771730267028623 22.042849819193993 2 16.180526420765215 20.806825450318783 38.88869784108338 24.123950287832624 3 20.280679793943477 23.853311966546762 29.96485484569833 25.901153393811427 4 13.672221572659685 16.71630088653824 35.94400159563127 33.66747594517081 5 14.139461946634846 37.22320995636011 33.77844416398144 14.8588839330236 6 33.09593733020627 36.006588856716434 16.162300460119535 14.735173352957764 7 28.567645824879467 33.23108454782424 20.05646608939662 18.14480353789968 8 26.849247233436728 34.699821867026145 19.062291519082237 19.38863938045489 9 26.659766019931634 16.61244729946285 19.679223092636025 37.04856358796949 10-11 25.576696905713327 26.50518924049327 26.45876462375427 21.459349230039134 12-13 26.587661182021556 23.700097839786917 27.392561081086658 22.319679897104873 14-15 23.34590809989202 25.33030255094672 25.6915548463861 25.63223450277516 16-17 22.63991003940934 28.426652544412885 26.348377201730433 22.585060214447342 18-19 22.430483435008973 27.22958520464659 28.821261786694365 21.518669573650076 20-21 23.342985068467712 25.832032297777808 29.14382690151791 21.681155732236565 22-23 22.863062691289247 25.75759138897486 29.20544035214416 22.173905567591735 24-25 23.149272157666502 25.92635260376009 29.19949379451221 21.724881444061197 26-27 22.74603799603499 25.98476238292809 29.284010818673735 21.98518880236319 28-29 22.30571355861092 26.680984380051036 29.113734687841752 21.899567373496296 30-31 22.635040449805274 26.348857002845673 28.75125733985574 22.264845207493316 32-33 22.541558629132652 26.388096040496023 28.82917116584249 22.241174164528843 34-35 22.942873649387536 26.39514570451935 28.707951333246218 21.954029312846895 36-37 23.205430648500315 26.12210018088406 28.79804947832486 21.87441969229076 38-39 22.728921825700457 26.504466243111498 28.894830506288848 21.871781424899197 40-41 22.651086336031693 26.45068330158119 28.817479040145255 22.080751322241863 42-43 22.8315990941029 26.700921545665434 28.14420065930324 22.323278700928423 44-45 22.937371472588843 26.63380262316279 28.055083667475945 22.373742236772422 46-47 23.28641581325612 27.060393268086685 27.746273994649133 21.906916924008062 48-49 23.25959270136248 27.170436804060603 27.32518552652393 22.244784968052986 50-51 22.914503050269264 27.342723715069774 27.541661794947625 22.201111439713337 52-53 22.957144920459157 27.755386974971973 26.926277708618475 22.3611903959504 54-55 22.897824576848215 27.677840670715355 26.999869323301027 22.424465429135402 56-57 23.1215224523202 27.552838091569974 26.999525437251116 22.32611401885871 58-59 23.10501592192411 27.113867548849015 27.234915438420327 22.54620109080655 60-61 22.657792114005105 27.43935569509687 27.73251855265239 22.17033363824563 62-63 22.462808723701315 27.42336499377566 27.73991210272564 22.37391417979738 64-65 22.415868277887437 27.696926346485828 27.62556999112774 22.261635384498994 66-67 22.27968940211971 27.151866957365005 27.60682820140718 22.961615439108098 68-69 22.372538635597707 27.05747023666238 27.638121831949764 22.931869295790147 70-71 22.517314662613398 27.26827238526242 27.404795147080062 22.809617805044123 72-73 22.36290982619999 26.884323610528416 27.483888938561318 23.268877624710278 74-75 22.224151805057875 27.26861627131234 27.207060668376926 23.30017125525286 76-77 22.145917728701416 26.788551345626114 27.541145965872747 23.524384959799722 78-79 21.833325309325502 26.747285019635893 27.33704959524612 24.082340075792487 80-81 22.09966505498738 26.592020468097694 27.43849597997208 23.86981849694285 82-83 22.00968044223974 26.97095007608518 27.56673573080461 23.452633750870465 84-85 21.344048859497995 27.220737775668603 27.163652495073816 24.27156086975959 86-87 21.49459755015578 26.71650721816819 26.922151076019453 24.86674415565658 88-89 21.3295322461949 26.485931621697834 27.336705709196202 24.847830422911063 90-91 21.351007908162828 25.512293848690824 27.802376747697107 25.334321495449235 92-93 21.225465331212657 25.624725959678457 27.720414391952886 25.429394317156 94-95 20.726700000687774 26.24228835533058 27.91649758935879 25.11451405462286 96-97 20.219296134033026 26.93814177734066 28.402408577893627 24.440153510732685 98-99 20.26953834937851 28.165003688184225 26.48477355924393 25.08068440319333 100-101 19.972588935491135 29.029589848620073 25.28094621481401 25.716875001074772 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 52.0 1 38.5 2 36.5 3 44.0 4 42.5 5 50.5 6 75.5 7 154.0 8 243.0 9 284.5 10 283.5 11 303.0 12 340.5 13 385.0 14 456.0 15 489.0 16 497.5 17 549.5 18 598.5 19 615.5 20 658.5 21 734.0 22 848.0 23 984.0 24 1114.0 25 1287.0 26 1575.5 27 1911.5 28 2211.0 29 2569.5 30 2894.5 31 3288.0 32 3781.0 33 4145.0 34 4452.5 35 4884.5 36 5481.0 37 6009.5 38 6599.0 39 7134.0 40 7555.5 41 8183.0 42 8574.0 43 9062.5 44 9624.0 45 9973.0 46 10672.5 47 11880.0 48 13215.5 49 13143.0 50 12025.0 51 11514.0 52 11430.5 53 11423.0 54 11344.5 55 10581.0 56 9287.0 57 8271.5 58 7572.0 59 6600.5 60 5233.0 61 4139.0 62 3230.5 63 2321.0 64 1628.0 65 1109.5 66 814.0 67 652.5 68 514.0 69 382.5 70 261.0 71 158.5 72 108.0 73 76.0 74 50.0 75 34.0 76 20.5 77 15.5 78 12.0 79 8.0 80 5.0 81 4.0 82 4.0 83 3.0 84 1.0 85 0.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05192679353769335 2 0.0 3 0.0 4 0.0 5 0.001719430249592495 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004298575623981238 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013755441996739961 24-25 0.001031658149755497 26-27 1.7194302495924951E-4 28-29 0.004126632599021988 30-31 5.158290748777485E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.158290748777485E-4 40-41 0.0 42-43 0.011176296622351218 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.158290748777485E-4 84-85 3.4388604991849903E-4 86-87 0.0 88-89 0.0 90-91 0.006361891923492231 92-93 0.0022352593244702435 94-95 0.0 96-97 0.0 98-99 1.7194302495924951E-4 100-101 0.012207954772106715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 290794.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.10964206765442 #Duplication Level Percentage of deduplicated Percentage of total 1 84.37543847209903 59.9990722905236 2 9.686515377004724 13.77609282683273 3 2.75217735857295 5.871190406244751 4 1.124576376618129 3.1987289447621956 5 0.6047080167240737 2.1500285312345038 6 0.3748518337484692 1.599334783575453 7 0.25344860782445644 1.2615847849459692 8 0.1599106221336317 0.9096949682190788 9 0.11706990551034724 0.7492319169961426 >10 0.510880879908957 6.1726983346937105 >50 0.02483795232068719 1.1760933305377874 >100 0.013636522842729953 1.65124057715079 >500 9.740373459092823E-4 0.6012067202042306 >1k 9.740373459092823E-4 0.8838015840790301 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1371 0.4714677744382621 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1181 0.4061294249537473 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 982 0.337696101019966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 754 0.2592900816385483 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 333 0.11451405462286016 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 308 0.10591690337489769 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.7194302495924951E-4 0.0 24-25 0.0 0.0 0.0 6.877720998369981E-4 0.0 26-27 0.0 0.0 0.0 0.0017194302495924952 0.0 28-29 0.0 0.0 0.0 0.004986347723818236 0.0 30-31 0.0 0.0 0.0 0.0173662455208842 0.0 32-33 0.0 0.0 0.0 0.032669174742257404 0.0 34-35 0.0 0.0 0.0 0.04418935741452712 0.0 36-37 0.0 0.0 0.0 0.06086783083557433 0.0 38-39 0.0 0.0 0.0 0.08408013920507301 0.0 40-41 0.0 0.0 0.0 0.1021341568257942 0.0 42-43 0.0 0.0 0.0 0.15337317826365054 0.0 44-45 0.0 0.0 0.0 0.20753523112581415 0.0 46-47 0.0 0.0 0.0 0.2771721562343102 0.0 48-49 0.0 0.0 0.0 0.357641491915239 0.0 50-51 0.0 0.0 0.0 0.4558209591669704 0.0 52-53 0.0 0.0 0.0 0.6475374319965337 0.0 54-55 0.0 0.0 0.0 0.8046933568092877 0.0 56-57 0.0 0.0 0.0 1.1310412181819434 0.0 58-59 0.0 0.0 0.0 1.5287454349126874 0.0 60-61 0.0 0.0 0.0 1.9238705062690427 0.0 62-63 0.0 0.0 0.0 2.5452725984717706 0.0 64-65 0.0 0.0 0.0 2.9457279036018624 0.0 66-67 0.0 0.0 0.0 3.6390021802375565 1.7194302495924951E-4 68-69 0.0 0.0 0.0 4.367868663039815 3.4388604991849903E-4 70-71 0.0 0.0 0.0 5.048075269778606 3.4388604991849903E-4 72-73 0.0 0.0 0.0 6.168971849487954 3.4388604991849903E-4 74-75 0.0 0.0 0.0 7.230891971636279 3.4388604991849903E-4 76-77 0.0 0.0 0.0 8.04401053666857 3.4388604991849903E-4 78-79 0.0 0.0 0.0 8.925390482609682 3.4388604991849903E-4 80-81 0.0 0.0 0.0 10.386906194763302 3.4388604991849903E-4 82-83 0.0 0.0 0.0 11.300955315446675 3.4388604991849903E-4 84-85 0.0 0.0 0.0 12.628871297206958 3.4388604991849903E-4 86-87 0.0 0.0 0.0 14.823380124761858 3.4388604991849903E-4 88-89 0.0 0.0 0.0 17.56587137286189 3.4388604991849903E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACGCG 15 6.615292E-4 94.991394 5 GCCGCGC 65 1.3264513E-4 36.535156 8 GCTCCGT 60 0.0039478904 31.6638 6 GGGCGTA 65 0.00583957 29.228125 9 TCATACC 35 0.008391924 27.140402 68-69 AGGGCGT 95 0.0012249976 24.997736 8 TACATGG 1095 0.0 23.856287 2 TCCTAGA 165 2.4214241E-6 23.02822 2 GTACATG 1125 0.0 22.817554 1 GAGTACT 1270 0.0 22.812895 12-13 TCCTACA 125 2.2516584E-4 22.797935 2 GTGTAGG 105 0.002182516 22.636465 1 CGTCTGC 105 0.0021934588 22.617002 8 TGCATAG 105 0.0021934588 22.617002 5 GTAGATC 105 0.0021934588 22.617002 3 TATACTG 105 0.0021934588 22.617002 5 AGAGTAC 1835 0.0 22.518396 10-11 AGTACTT 1335 0.0 22.235815 12-13 ACTGGTC 150 3.0252837E-5 22.16466 8 CCTAGAC 130 2.9359947E-4 21.921093 3 >>END_MODULE