FastQCFastQC Report
Thu 26 May 2016
SRR937303_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937303_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538861
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43680.81059865160032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.599783617667636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21420.3975051079963108No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19250.357234982676423No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17960.33329559942174325No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15790.2930254741018556No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG10110.1876179571355136No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9240.17147279168468305No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA8940.16590549325336218No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT8790.16312184403770175No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8750.1623795375801923No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8440.15662666253449406No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.15477089639072045No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7920.14697667858687122No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6270.11635653721460636No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5710.10596424680947407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5540.1028094443650589No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACGC550.002578507434.5451858
GTCGGGA1308.247298E-932.893542
ACACGGG753.071392E-431.6752593
TACCTGG4650.029.6316952
GTACTAC650.005833629.23873
GTACACG1153.8402377E-628.931661
GTACATG41100.028.4489611
TACATGG41600.027.9823512
TCGGGAG1554.5658453E-827.5881293
CGGGAGT1606.2123945E-826.7260024
ACATGGG42050.026.5529823
GGTCGGG1701.1135489E-725.1632481
GAGTACT32500.024.62672812-13
AGTACTT34000.024.4483412-13
ATGGGAT12900.024.308925
CATGGGG9750.023.8782734
CATGGGA32500.023.5371534
GTACTTT34750.023.10053814-15
GCTAGAC1252.249596E-422.8061873
GTGTAGC3150.022.6336121
Produced by FastQC (version 0.11.5)