##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937303_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 538861 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03600928625379 33.0 31.0 34.0 31.0 34.0 2 32.15101482571572 33.0 31.0 34.0 31.0 34.0 3 32.17435850803825 34.0 31.0 34.0 30.0 34.0 4 35.768593384936004 37.0 35.0 37.0 35.0 37.0 5 35.78621388447113 37.0 35.0 37.0 35.0 37.0 6 35.85591274929899 37.0 35.0 37.0 35.0 37.0 7 35.81334332972696 37.0 35.0 37.0 35.0 37.0 8 35.808058107749495 37.0 35.0 37.0 35.0 37.0 9 37.45225948806835 39.0 37.0 39.0 35.0 39.0 10-11 37.349112294265126 39.0 37.0 39.0 35.0 39.0 12-13 37.23914887141582 39.0 37.0 39.0 34.5 39.0 14-15 38.35132158385929 40.0 38.0 41.0 34.0 41.0 16-17 38.30242771326928 40.0 38.0 41.0 34.0 41.0 18-19 38.30385479743384 40.0 38.0 41.0 34.0 41.0 20-21 38.22564538907065 40.0 38.0 41.0 34.0 41.0 22-23 38.29211243715912 40.0 38.0 41.0 34.0 41.0 24-25 38.27296556997074 40.0 38.0 41.0 34.0 41.0 26-27 38.16672388612277 40.0 38.0 41.0 34.0 41.0 28-29 38.02056096098994 40.0 38.0 41.0 34.0 41.0 30-31 37.75146930284433 40.0 37.0 41.0 33.0 41.0 32-33 37.483728642451396 40.0 36.5 41.0 33.0 41.0 34-35 37.47106025487092 40.0 37.0 41.0 33.0 41.0 36-37 37.320471327485194 40.0 36.5 41.0 33.0 41.0 38-39 37.16562805621487 40.0 36.0 41.0 32.5 41.0 40-41 36.92011205115976 39.5 36.0 41.0 31.5 41.0 42-43 36.625984251968504 39.0 36.0 41.0 31.0 41.0 44-45 36.30508053097181 39.0 35.5 41.0 30.0 41.0 46-47 35.757425384282776 39.0 35.0 41.0 28.5 41.0 48-49 35.32279567458028 39.0 35.0 40.0 26.5 41.0 50-51 34.352266168826475 38.0 34.0 39.5 24.5 40.5 52-53 34.43171151744142 38.0 34.5 39.5 24.0 40.5 54-55 34.73571755981598 39.0 35.0 40.0 24.0 41.0 56-57 34.50378297928408 38.0 34.5 40.0 23.0 41.0 58-59 34.27042966553527 38.0 34.0 40.0 22.5 41.0 60-61 33.93570605406589 38.0 34.0 40.0 21.0 41.0 62-63 33.56661365361383 38.0 33.5 40.0 18.5 41.0 64-65 33.151940853021465 37.0 33.0 40.0 16.0 41.0 66-67 32.72354744544512 36.5 32.5 39.0 11.0 41.0 68-69 32.23734692249022 36.0 32.0 39.0 7.5 40.5 70-71 31.75713774053049 35.5 31.0 39.0 4.5 40.0 72-73 31.249092066414157 35.0 31.0 38.0 2.0 40.0 74-75 30.693957254282644 35.0 30.0 37.5 2.0 39.5 76-77 30.162450056693658 34.5 29.5 37.0 2.0 39.0 78-79 29.60781537353789 34.0 29.0 36.0 2.0 39.0 80-81 29.012298162234785 34.0 29.0 36.0 2.0 37.5 82-83 28.444920860852797 34.0 27.5 35.0 2.0 37.0 84-85 27.85457845344161 34.0 26.5 35.0 2.0 36.5 86-87 27.259225477442236 33.0 26.0 35.0 2.0 36.0 88-89 26.688842762790404 33.0 25.0 35.0 2.0 36.0 90-91 26.101769287441474 32.0 24.0 35.0 2.0 35.0 92-93 25.444675899721823 32.0 20.5 34.5 2.0 35.0 94-95 24.689772501628436 31.5 16.5 34.0 2.0 35.0 96-97 23.85002625909093 31.0 4.5 34.0 2.0 35.0 98-99 22.675855183433207 31.0 2.0 34.0 2.0 35.0 100-101 19.58496161347732 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23664415273859873 1101 2 0.397694806868671 1101 3 0.3063560235329845 1101 4 0.15611603616430614 1101 5 0.1805542743899693 1101 6 0.1937948047064424 1101 7 0.300456533527246 1101 8 0.14859293605153567 1101 9 0.13182060845816324 1101 10-11 0.1283222784941529 1101 12-13 0.23917131424767035 1101 14-15 0.40311921245515947 1101 16-17 0.511140200339689 1101 18-19 0.5294538865754745 1101 20-21 0.26638643976780685 1101 22-23 0.4039835120371791 1101 24-25 0.45856969842301964 1101 26-27 0.4807759836481509 1101 28-29 0.42630067140876804 1101 30-31 0.6030155556401411 1101 32-33 0.5464864552869813 1101 34-35 0.6687672271058176 1101 36-37 0.7984024797911999 1101 38-39 0.9290452606225443 1101 40-41 0.973546747380972 1101 42-43 0.7951697192217466 1101 44-45 0.7994241132871878 1101 46-47 1.0208532972358242 1101 48-49 0.9134745346948492 1101 50-51 1.2542043761208106 1101 52-53 1.098079651976576 1101 54-55 1.2085105817792154 1101 56-57 1.2461866738171778 1101 58-59 1.1951655019949428 1101 60-61 1.0006177577700512 1101 62-63 1.117196947745839 1101 64-65 1.0910100148164972 1101 66-67 1.13070225290803 1101 68-69 1.1296350036524672 1101 70-71 1.0917969751295793 1101 72-73 1.2125671066763672 1101 74-75 1.280630734263891 1101 76-77 0.9861213598873597 1101 78-79 1.1491099005037348 1101 80-81 1.3776142756214718 1101 82-83 1.2259347983119078 1101 84-85 1.185740774640525 1101 86-87 1.0389879648812759 1101 88-89 0.8826931820129253 1101 90-91 0.9358934721390746 1101 92-93 1.3020520401863642 1101 94-95 1.6196936038729781 1101 96-97 1.5174260059161142 1101 98-99 1.4396028165658628 1101 100-101 1.9127614642655288 1102 1 0.23200462786242326 1102 2 0.1912131512308477 1102 3 0.06980295113017831 1102 4 0.11520386225622303 1102 5 0.3822482139393557 1102 6 0.18205350096575046 1102 7 0.21852389213287893 1102 8 0.17625339578627575 1102 9 0.24264483658143376 1102 10-11 0.313666737567722 1102 12-13 0.2725599343024996 1102 14-15 0.5086171064300444 1102 16-17 0.5441526427018601 1102 18-19 0.48073317571363106 1102 20-21 0.5062424663054301 1102 22-23 0.4846394291413034 1102 24-25 0.5410694561873797 1102 26-27 0.4703199536907263 1102 28-29 0.4107633927693968 1102 30-31 0.42788204105755057 1102 32-33 0.40900490794274447 1102 34-35 0.4492019973239465 1102 36-37 0.6814796133171868 1102 38-39 0.7396996388179176 1102 40-41 0.8832071045217873 1102 42-43 1.004592400456751 1102 44-45 1.1236351375734515 1102 46-47 1.1851289556892795 1102 48-49 1.5273594813175109 1102 50-51 1.039657698738779 1102 52-53 1.3173969464610167 1102 54-55 1.2751909276347035 1102 56-57 1.5013320436967348 1102 58-59 1.2628204900541462 1102 60-61 1.3274477767499278 1102 62-63 1.193087947409559 1102 64-65 0.7893326042869333 1102 66-67 1.0502287249882372 1102 68-69 1.3825033585590205 1102 70-71 1.5815831808075735 1102 72-73 1.770646662558704 1102 74-75 1.339981314774466 1102 76-77 1.4067535663975157 1102 78-79 1.3153946898157294 1102 80-81 1.9862897311669556 1102 82-83 1.6101803625499471 1102 84-85 1.6205508904006614 1102 86-87 1.6540840896810742 1102 88-89 1.8771331617221314 1102 90-91 1.770805611849191 1102 92-93 1.7517867209197995 1102 94-95 1.4523458275961083 1102 96-97 1.7444235949590805 1102 98-99 2.227723581004536 1102 100-101 2.0216107595023196 1103 1 0.3110409654876065 1103 2 0.4272554974397238 1103 3 0.31687820148783175 1103 4 0.38229399101158634 1103 5 0.23412346429700648 1103 6 0.27899427349794337 1103 7 0.3161994286135652 1103 8 0.3383066146703939 1103 9 0.3607884703439552 1103 10-11 0.3229634471528513 1103 12-13 0.38869269538976425 1103 14-15 0.6441890663728174 1103 16-17 0.6661532149479257 1103 18-19 0.6033460512501136 1103 20-21 0.4560278740307453 1103 22-23 0.30998191841168676 1103 24-25 0.17875987106578606 1103 26-27 0.4232790380970144 1103 28-29 0.42010359305551503 1103 30-31 0.4661401240332381 1103 32-33 0.5635605588726378 1103 34-35 0.6678801089119304 1103 36-37 0.700259584704888 1103 38-39 0.6680635873157641 1103 40-41 0.8009284206877396 1103 42-43 0.5703835306427294 1103 44-45 0.6539962248409736 1103 46-47 0.8344554220698299 1103 48-49 1.1442453182273837 1103 50-51 1.1582659963067314 1103 52-53 1.2743983770761815 1103 54-55 1.4780224011683245 1103 56-57 1.469990126672414 1103 58-59 1.4208611195248224 1103 60-61 1.4853625120861196 1103 62-63 1.6140893780247225 1103 64-65 1.206189828893521 1103 66-67 1.482816993943885 1103 68-69 1.608261298473181 1103 70-71 1.799327386816163 1103 72-73 1.7362558184957635 1103 74-75 1.597703775432553 1103 76-77 1.6222637237651831 1103 78-79 1.7363938314466303 1103 80-81 1.4417449529123054 1103 82-83 1.4918375871565281 1103 84-85 1.7108764984121088 1103 86-87 1.7228955346023902 1103 88-89 2.0680127039252767 1103 90-91 2.0188937377433263 1103 92-93 1.84885682106286 1103 94-95 1.983073152345753 1103 96-97 1.741157214415452 1103 98-99 2.0678025509616162 1103 100-101 1.7426270685151977 1104 1 0.2374409654876075 1104 2 0.05316318974741563 1104 3 0.11478589379552062 1104 4 0.21367860639620062 1104 5 0.14261577198931263 1104 6 0.046748119651788045 1104 7 0.0683225055366421 1104 8 0.10581430697808258 1104 9 0.0478038549593407 1104 10-11 0.358286524075929 1104 12-13 0.21780038769746568 1104 14-15 0.6620659894497365 1104 16-17 0.41301475340946325 1104 18-19 0.4228229743270404 1104 20-21 0.021412489415361335 1104 22-23 0.30207422610398993 1104 24-25 0.30128294798885946 1104 26-27 0.23706365348163416 1104 28-29 0.3889035930555167 1104 30-31 0.2885401240332399 1104 32-33 0.50026825118033 1104 34-35 0.3431108781426957 1104 36-37 0.32592112316641675 1104 38-39 0.509078971931153 1104 40-41 0.7873591899185115 1104 42-43 0.9029989152581166 1104 44-45 1.0617808402255875 1104 46-47 1.1113477297621372 1104 48-49 1.1035991643812295 1104 50-51 1.187004457845191 1104 52-53 1.1322445309223355 1104 54-55 1.5102993242452456 1104 56-57 1.5104516651339566 1104 58-59 1.772799581063282 1104 60-61 1.828531742855347 1104 62-63 1.769473993409342 1104 64-65 1.5763129058166 1104 66-67 1.824324686251579 1104 68-69 1.6153689907808726 1104 70-71 1.5325273868161595 1104 72-73 1.4339788954188322 1104 74-75 1.392626852355626 1104 76-77 1.4600175699190316 1104 78-79 1.5240246006773965 1104 80-81 1.6957141836815346 1104 82-83 1.687929894848839 1104 84-85 1.5330918830274918 1104 86-87 1.831326303833162 1104 88-89 1.772043473156046 1104 90-91 1.9417245069740936 1104 92-93 1.687010667216704 1104 94-95 1.7537808446534449 1104 96-97 1.90872644518468 1104 98-99 2.097617935577002 1104 100-101 2.139180914669044 1105 1 0.12943415117755563 1105 2 0.17424561669014338 1105 3 0.30874736673484904 1105 4 0.12497201486166887 1105 5 0.22936980160535825 1105 6 0.10778337060185805 1105 7 0.3830178272507041 1105 8 0.1444776538526753 1105 9 0.12233668158756927 1105 10-11 0.1391828679364906 1105 12-13 0.28119968005756846 1105 14-15 0.2740997989111449 1105 16-17 0.2887780871796153 1105 18-19 0.30507824886510804 1105 20-21 0.16437540384335847 1105 22-23 0.3299355811033351 1105 24-25 0.2762167705547043 1105 26-27 0.33053764116345974 1105 28-29 0.34059013479316036 1105 30-31 0.5733782841695145 1105 32-33 0.6089656696051833 1105 34-35 0.5765206540567291 1105 36-37 0.5369655472261741 1105 38-39 0.6366441665321219 1105 40-41 0.6381795280131257 1105 42-43 0.7435624062815762 1105 44-45 0.7991560204116723 1105 46-47 0.8896009075894185 1105 48-49 0.6530330524692971 1105 50-51 0.7562326061874813 1105 52-53 0.9928447143845318 1105 54-55 1.1418561320030776 1105 56-57 1.2004909784611755 1105 58-59 1.0119766220801907 1105 60-61 1.2828562088493172 1105 62-63 1.1504777936976396 1105 64-65 1.3796453655204317 1105 66-67 1.4851515765673966 1105 68-69 1.1572180276043582 1105 70-71 1.2723399932897586 1105 72-73 1.5875498559744656 1105 74-75 1.5041364841207958 1105 76-77 1.504817727172341 1105 78-79 1.2133628263358993 1105 80-81 1.4112141181593252 1105 82-83 1.3214471271905985 1105 84-85 1.5798904677477132 1105 86-87 1.6701023489124402 1105 88-89 1.4865435386782586 1105 90-91 1.249768013722882 1105 92-93 1.6178925961906252 1105 94-95 1.6038240893133846 1105 96-97 1.739251933325157 1105 98-99 1.628283641251226 1105 100-101 1.7021241724334253 1106 1 -0.06189678285676692 1106 2 -0.6971135974830176 1106 3 -0.21866970138192698 1106 4 -0.05724684784050993 1106 5 -0.05412730832992452 1106 6 0.04580398652222328 1106 7 0.11219413060032224 1106 8 -0.12397682903822016 1106 9 -0.10087333981056901 1106 10-11 -0.18163876035266924 1106 12-13 -0.3687677902614439 1106 14-15 -0.08090055835124588 1106 16-17 -0.02864766805427621 1106 18-19 0.03698666935165562 1106 20-21 -0.0909919935189194 1106 22-23 -0.037781436996702666 1106 24-25 -0.015463528492716705 1106 26-27 0.10931700719194026 1106 28-29 0.030537147139398257 1106 30-31 0.05189553242176004 1106 32-33 -0.05382752059755802 1106 34-35 0.14551366299581048 1106 36-37 0.06061631759671826 1106 38-39 0.2157439405166528 1106 40-41 -0.2557567890252841 1106 42-43 -0.26325002344115234 1106 44-45 -0.12572298045704144 1106 46-47 0.1448253999947653 1106 48-49 0.27839454560045596 1106 50-51 -0.09762451141953932 1106 52-53 0.02943590466999524 1106 54-55 0.13210098836479034 1106 56-57 -0.2554491669258141 1106 58-59 -0.1388934058614879 1106 60-61 0.04445125380797066 1106 62-63 0.20314015065165592 1106 64-65 0.39340748893766886 1106 66-67 0.5117300182264515 1106 68-69 0.780787567788849 1106 70-71 0.33634637136362144 1106 72-73 0.5153339641911217 1106 74-75 0.4538715458519782 1106 76-77 0.7481021343612113 1106 78-79 0.8440212045150624 1106 80-81 1.0162473811683803 1106 82-83 1.487985931527387 1106 84-85 0.9510372048138755 1106 86-87 1.0888063513794322 1106 88-89 1.172901004146027 1106 90-91 1.1588955037477398 1106 92-93 1.5533954524094362 1106 94-95 1.2763517395422177 1106 96-97 0.5281117398017621 1106 98-99 0.7298458180697835 1106 100-101 0.2735127197293252 1107 1 0.16725840970666894 1107 2 0.1842632053504687 1107 3 0.12706237998681758 1107 4 0.2235272567316713 1107 5 0.09265165902317563 1107 6 0.1869634418549424 1107 7 0.30442174098678976 1107 8 0.030382882418869883 1107 9 0.26416210117560013 1107 10-11 0.33929448163561204 1107 12-13 0.16322089011593732 1107 14-15 0.3146280116060822 1107 16-17 0.3286100100797711 1107 18-19 0.33538499648337705 1107 20-21 0.19882706267171812 1107 22-23 0.19180585350296298 1107 24-25 0.06444100696841559 1107 26-27 0.1985553058454954 1107 28-29 0.3269563313922319 1107 30-31 0.29726223356670545 1107 32-33 0.30067861161097653 1107 34-35 0.49669958152856 1107 36-37 0.23764214048581067 1107 38-39 0.23470699502367864 1107 40-41 0.4133211184565013 1107 42-43 0.2172070600544984 1107 44-45 0.417450991575258 1107 46-47 0.7520327040170898 1107 48-49 0.37344937502251696 1107 50-51 0.6561759354548116 1107 52-53 0.3966945231208072 1107 54-55 0.6262284863610219 1107 56-57 0.647588503954367 1107 58-59 0.35787369558972415 1107 60-61 0.5376205242565035 1107 62-63 0.8829331914121425 1107 64-65 0.3070774556683702 1107 66-67 0.5890352495219773 1107 68-69 0.8724527792033996 1107 70-71 0.7739675490879669 1107 72-73 0.7976237698142157 1107 74-75 0.8943853170146987 1107 76-77 1.121186238978165 1107 78-79 0.8734956570044403 1107 80-81 0.761983492466058 1107 82-83 0.758756856933406 1107 84-85 0.752654997397908 1107 86-87 1.1799454331825139 1107 88-89 1.0820216288745677 1107 90-91 0.9180109791226343 1107 92-93 1.0968000259310138 1107 94-95 1.1386333957534625 1107 96-97 0.9461519405817782 1107 98-99 1.4351729363571515 1107 100-101 1.9815869062433897 1108 1 0.19038214195819592 1108 2 0.24379667391031035 1108 3 -0.0457570926298132 1108 4 0.17535281454100016 1108 5 0.2637469937087715 1108 6 0.25765309702735095 1108 7 0.11963472273121312 1108 8 0.19468308525862454 1108 9 0.26436494093218954 1108 10-11 0.2616575647999113 1108 12-13 0.40196328362506506 1108 14-15 0.4528125957845859 1108 16-17 0.2774943914185144 1108 18-19 0.3735695806618793 1108 20-21 0.3927925799130989 1108 22-23 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-1.1820847147112588 2316 54-55 -1.2109901592181487 2316 56-57 -1.4075750907188862 2316 58-59 -1.4084490254027102 2316 60-61 -1.3483727536143704 2316 62-63 -1.2001135205260027 2316 64-65 -1.1706101711064818 2316 66-67 -1.045809093012636 2316 68-69 -1.1520594744736812 2316 70-71 -1.0956368643915688 2316 72-73 -1.0448765486540026 2316 74-75 -0.9127503308476435 2316 76-77 -1.548503425993271 2316 78-79 -0.6849163134809046 2316 80-81 -0.34394393597657924 2316 82-83 0.2830878287024241 2316 84-85 0.772341232711625 2316 86-87 0.7186172737328285 2316 88-89 -0.149602755011923 2316 90-91 -0.04750626225667531 2316 92-93 0.05013218338169878 2316 94-95 -0.44113554330641946 2316 96-97 -0.6661007565990431 2316 98-99 -0.011780057734032567 2316 100-101 0.7980512231045687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2031.0 3 261.0 4 257.0 5 278.0 6 344.0 7 393.0 8 471.0 9 623.0 10 751.0 11 1149.0 12 1770.0 13 2656.0 14 2839.0 15 4187.0 16 5734.0 17 8493.0 18 6916.0 19 3885.0 20 3666.0 21 3668.0 22 4035.0 23 4552.0 24 5201.0 25 5739.0 26 6790.0 27 7793.0 28 9387.0 29 10955.0 30 13694.0 31 16865.0 32 21819.0 33 28564.0 34 38757.0 35 54878.0 36 76570.0 37 100890.0 38 74297.0 39 7703.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.58335659910288 19.570793083552033 14.969009994974595 20.8768403223705 2 14.325556818668101 21.146736673284053 41.910012223869856 22.617694284177997 3 19.199991069252366 27.43414038763166 30.749494389443853 22.61637415367212 4 11.351843134920118 17.02962523326406 39.94857492636949 31.669956705446335 5 11.936294118741511 39.12869555510075 34.70965821348168 14.225352112676056 6 27.923205975679167 37.64484437715523 19.438254911224114 14.993694735941492 7 26.109001089936502 34.97078002998255 21.616081957273 17.304136922807945 8 25.44806934007886 33.87712967785135 21.06000297596905 19.614798006100738 9 26.79048102697464 16.757164993424222 21.845905439014544 34.60644854058659 10-11 23.35955173234909 27.558619329682106 28.22992320514347 20.851905732825337 12-13 25.779532963838967 25.482089692446635 28.5817127263494 20.156664617364996 14-15 22.776800229155583 25.970470178153587 28.746563596254642 22.506165996436184 16-17 19.728263143945206 29.798708273466595 29.386732136463607 21.08629644612459 18-19 19.591350177229124 27.958684971044516 32.910965145558485 19.53899970616787 20-21 21.063257191106945 26.64810537161647 32.293770621078046 19.99486681619854 22-23 20.171656259734704 26.861507990013067 32.154211669092106 20.812624081160124 24-25 19.993843985006105 26.89750792982143 32.77492139921943 20.333726685953035 26-27 20.282860968533807 26.604180614446182 32.936379528379085 20.17657888864093 28-29 19.6322270509252 26.909009070170363 33.32945838444391 20.129305494460528 30-31 19.78086778589034 27.15602474073385 32.985257870994744 20.07784960238106 32-33 19.383576613484493 27.302403660595125 32.615939771305804 20.698079954614574 34-35 20.01313190308432 26.78685024050355 32.70094973791995 20.499068118492175 36-37 20.23967832108005 26.779639896666968 32.68353422767116 20.297147554581823 38-39 20.29765618534797 26.86455862512076 32.724606659978114 20.113178529553153 40-41 19.966397087127685 27.045602890557152 32.94331391294484 20.04468610937032 42-43 19.500217595881672 27.554333877633002 32.28778822155352 20.657660304931802 44-45 19.849619585533276 27.759748411297686 31.595044104273178 20.79558789889586 46-47 19.983353869760307 27.718596703322252 30.93539162578755 21.36265780112989 48-49 20.09220155628489 27.765279427260374 30.847158761705384 21.295360254749347 50-51 20.660555134933023 28.062106208946673 30.56711492420423 20.710223731916074 52-53 20.163964773527493 28.36945935160146 30.536369277023816 20.930206597847228 54-55 19.76304913117395 28.129150220787096 30.8518810614149 21.25591958662406 56-57 20.353456507852677 28.712506729471972 29.8152568171062 21.11877994556915 58-59 20.276793317986915 28.079850948862223 30.2385450407399 21.404810692410962 60-61 20.434395349788577 27.847715245210885 30.63276569316191 21.085123711838627 62-63 20.788117887603804 27.8182499472338 30.588126999653188 20.80550516550922 64-65 20.27896935855456 27.92473424184782 30.81158272297746 20.984713676620164 66-67 20.021421936844337 28.02619724359922 30.553037424235242 21.399343395321203 68-69 19.9320350888611 28.37000432334934 30.528145933959678 21.16981465382988 70-71 20.00291953700234 28.20096084380198 30.113908432534053 21.682211186661622 72-73 20.047493702993275 27.93592999048824 30.017266211131265 21.999310095387216 74-75 19.913209796302446 28.173249388405225 30.103015713173903 21.810525102118426 76-77 20.090964970767903 27.811828421950253 30.35758290952138 21.739623697760468 78-79 20.001841242150313 27.583667252206467 30.500176219892666 21.91431528575055 80-81 19.870262951852602 28.385558570437585 30.082034134085173 21.66214434362464 82-83 19.83778526585141 28.31319363860871 29.95189160641495 21.897129489124936 84-85 19.843609531432886 27.73639160277453 29.841554942587724 22.578443923204862 86-87 19.95154269393614 27.74989633557893 30.121533749974432 22.177027220510496 88-89 20.42475562279112 26.796089964657938 30.268184983760875 22.510969428790066 90-91 19.889111319181062 26.96484309986211 30.69234295341764 22.453702627539187 92-93 19.921373399177842 27.216928894810888 30.650612805833394 22.211084900177877 94-95 19.343681253800508 27.18634996848019 30.59578954806725 22.874179229652057 96-97 19.167244736465264 27.156882254156056 30.531849011268175 23.1440239981105 98-99 19.33275556811926 27.55279856575365 29.9937326112508 23.12071325487629 100-101 19.944884217807754 27.19268395636109 30.31832897278984 22.544102853041316 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 874.0 1 514.0 2 187.5 3 225.5 4 198.5 5 189.0 6 299.0 7 598.0 8 1019.0 9 1304.0 10 1255.5 11 1093.0 12 1088.0 13 1142.5 14 1211.0 15 1286.0 16 1334.0 17 1405.0 18 1501.5 19 1588.0 20 1700.0 21 1901.0 22 2168.0 23 2520.0 24 2883.0 25 3415.5 26 4273.0 27 5272.5 28 6078.0 29 7120.5 30 8494.0 31 9696.5 32 10966.5 33 12437.0 34 14197.5 35 15550.0 36 16984.5 37 18601.5 38 18950.0 39 18604.0 40 18752.0 41 18938.0 42 18758.0 43 19073.0 44 19750.0 45 19975.0 46 19890.5 47 21162.5 48 26851.0 49 26165.5 50 19644.5 51 16621.5 52 13966.0 53 12182.0 54 11010.0 55 9806.5 56 8502.0 57 7286.5 58 6398.5 59 5664.5 60 4789.0 61 3856.0 62 2982.5 63 2161.0 64 1404.5 65 987.0 66 714.0 67 478.5 68 337.0 69 262.5 70 197.0 71 147.5 72 108.5 73 94.5 74 75.5 75 53.0 76 40.5 77 26.5 78 15.0 79 12.0 80 7.0 81 5.5 82 6.0 83 2.5 84 1.0 85 0.5 86 1.5 87 1.5 88 0.0 89 0.5 90 2.5 91 2.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2952523934743839 2 0.25758034075577935 3 0.25832264721328874 4 0.2560957278407604 5 0.2581370705989114 6 0.22547558646849558 7 0.22547558646849558 8 0.22658904615475975 9 0.2379092196317789 10-11 0.2458890140500055 12-13 0.2374452780958355 14-15 0.22918711875604286 16-17 0.22426933847504274 18-19 0.21137176377581604 20-21 0.21953713480841996 22-23 0.21499050775617456 24-25 0.21814531020058975 26-27 0.21174291700457076 28-29 0.22631068123319373 30-31 0.23865152608928833 32-33 0.2307645199782504 34-35 0.22992942521355228 36-37 0.2193515581940426 38-39 0.209052056096099 40-41 0.20599004195887252 42-43 0.21656790897838216 44-45 0.21489771944898595 46-47 0.2224135723312691 48-49 0.22909433044885416 50-51 0.24087844546181666 52-53 0.2537760201610434 54-55 0.22992942521355228 56-57 0.20682513672357064 58-59 0.196525634625627 60-61 0.20023716691317428 62-63 0.20626840688043857 64-65 0.19671121124000437 66-67 0.20311360443602341 68-69 0.2010722616778724 70-71 0.204969370579797 72-73 0.20515494719417438 74-75 0.20923763271047635 76-77 0.21925876988685392 78-79 0.2190731932724766 80-81 0.21489771944898595 82-83 0.20728907825951406 84-85 0.1930924672596458 86-87 0.19819582415502326 88-89 0.20719628995232536 90-91 0.20682513672357064 92-93 0.20960878593923107 94-95 0.20487658227260833 96-97 0.21378425976272172 98-99 0.21471214283460857 100-101 0.20116504998506107 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 538861.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.81957281316477 #Duplication Level Percentage of deduplicated Percentage of total 1 84.73543062530918 55.7724984589741 2 9.56046367982492 12.585312706037072 3 2.517458641120719 4.970941570001281 4 1.035475174070863 2.726181344639266 5 0.5174129212010474 1.7027948720732315 6 0.32433059515869017 1.2808380734150686 7 0.2181507413613392 1.0051012023694978 8 0.16745016206255722 0.881719850756218 9 0.13132175482453168 0.7779187623263252 >10 0.7145171415874962 8.628658267827657 >50 0.050579541875312 2.2792408605514294 >100 0.022322811408898274 2.8561646681059263 >500 0.003108239563264196 1.5470194510193789 >1k 0.001977970631168125 2.9856099119035933 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4368 0.81059865160032 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3232 0.599783617667636 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2142 0.3975051079963108 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1925 0.357234982676423 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1796 0.33329559942174325 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1579 0.2930254741018556 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1011 0.1876179571355136 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 924 0.17147279168468305 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 894 0.16590549325336218 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 879 0.16312184403770175 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 875 0.1623795375801923 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 844 0.15662666253449406 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 834 0.15477089639072045 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 792 0.14697667858687122 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 627 0.11635653721460636 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 571 0.10596424680947407 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 554 0.1028094443650589 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.27883071886813E-5 0.0 20-21 0.0 0.0 0.0 1.855766143773626E-4 0.0 22-23 0.0 0.0 0.0 4.6394153594340657E-4 0.0 24-25 0.0 0.0 0.0 9.278830718868131E-4 0.0 26-27 0.0 0.0 0.0 0.0017629778365849448 0.0 28-29 0.0 0.0 0.0 0.0033403790587925274 0.0 30-31 0.0 0.0 0.0 0.007515852882283186 0.0 32-33 0.0 0.0 0.0 0.013083151313604064 0.0 34-35 0.0 0.0 0.0 0.01762977836584945 0.0 36-37 0.0 0.0 0.0 0.024403324790623182 0.0 38-39 0.0 0.0 0.0 0.03238311920884978 0.0 40-41 0.0 0.0 0.0 0.042033103156472636 0.0 42-43 0.0 0.0 0.0 0.05947730490794473 0.0 44-45 0.0 0.0 0.0 0.08221044016917164 0.0 46-47 0.0 0.0 0.0 0.1380690010967578 0.0 48-49 0.0 0.0 0.0 0.2643538871805531 0.0 50-51 0.0 0.0 0.0 0.3548224866895173 0.0 52-53 0.0 0.0 0.0 0.44612618096317974 0.0 54-55 0.0 0.0 0.0 0.5728750085829184 0.0 56-57 0.0 0.0 0.0 0.6719729206604301 0.0 58-59 0.0 0.0 0.0 0.7990001132017348 0.0 60-61 0.0 0.0 0.0 1.027723290421834 0.0 62-63 0.0 0.0 0.0 1.1790610194465736 0.0 64-65 0.0 0.0 0.0 1.3565650510985208 0.0 66-67 0.0 0.0 0.0 1.6625808882067918 0.0 68-69 0.0 0.0 0.0 1.9927216851841196 0.0 70-71 0.0 0.0 0.0 2.335574480246297 0.0 72-73 0.0 0.0 0.0 2.7460699512490234 0.0 74-75 0.0 0.0 0.0 3.094675621356899 0.0 76-77 0.0 0.0 0.0 3.3672876678772448 0.0 78-79 0.0 0.0 0.0 3.712831323847894 0.0 80-81 0.0 0.0 0.0 4.121749393628413 0.0 82-83 0.0 0.0 0.0 4.5329871710886485 0.0 84-85 0.0 0.0 0.0 4.963988857980073 0.0 86-87 0.0 0.0 0.0 5.777371158796053 0.0 88-89 0.0 0.0 0.0 6.955782660092305 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAACGC 55 0.0025785074 34.545185 8 GTCGGGA 130 8.247298E-9 32.89354 2 ACACGGG 75 3.071392E-4 31.675259 3 TACCTGG 465 0.0 29.631695 2 GTACTAC 65 0.0058336 29.2387 3 GTACACG 115 3.8402377E-6 28.93166 1 GTACATG 4110 0.0 28.448961 1 TACATGG 4160 0.0 27.982351 2 TCGGGAG 155 4.5658453E-8 27.588129 3 CGGGAGT 160 6.2123945E-8 26.726002 4 ACATGGG 4205 0.0 26.552982 3 GGTCGGG 170 1.1135489E-7 25.163248 1 GAGTACT 3250 0.0 24.626728 12-13 AGTACTT 3400 0.0 24.44834 12-13 ATGGGAT 1290 0.0 24.30892 5 CATGGGG 975 0.0 23.878273 4 CATGGGA 3250 0.0 23.537153 4 GTACTTT 3475 0.0 23.100538 14-15 GCTAGAC 125 2.249596E-4 22.806187 3 GTGTAGC 315 0.0 22.633612 1 >>END_MODULE