FastQCFastQC Report
Thu 26 May 2016
SRR937303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937303_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538861
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53210.9874531651019465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42020.7797929336136777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27300.5066241572501999No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25610.4752617094204257No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19800.36744169646717795No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17600.32661484130415824No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12790.2373524897886468No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11290.2095159976320424No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7840.1454920656718523No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG7680.1425228398418145No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7340.13621323495298415No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG7090.1315738195935501No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG7060.131017089750418No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.1256353679334745No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA6730.12489306147596504No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT6490.12043922273090833No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6380.11839787997275734No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6110.11338731138456856No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG804.4778312E-429.7018321
TACCTGG4900.029.0821722
GTACCCG700.00836795727.1559621
TATGGCG700.00838692527.143364
GTACATG49150.022.6253621
ACCTGGG6300.022.6194673
GAGTACT41600.022.60859312-13
AGTACTT42050.022.53608912-13
TACATGG49600.022.2181532
TCTATAC1503.0289228E-522.1670783
GTACTTT43850.021.28405214-15
CATGGGT4300.020.9887624
ACATGGG49650.020.8563793
GGTATAG1404.831383E-420.3669721
CATATAG2108.5887405E-720.3575213
ATAGGGT1404.8465314E-420.3575213
GTATAGC1951.0266915E-519.4965861
ATGGGTA2451.7513412E-719.3881155
TACTTTT49200.019.3075114-15
GTACCTG7400.019.2660541
Produced by FastQC (version 0.11.5)