##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937303_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 538861 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.483454916945185 33.0 31.0 34.0 31.0 34.0 2 32.67303813042695 34.0 31.0 34.0 31.0 34.0 3 32.72938846938264 34.0 31.0 34.0 31.0 34.0 4 36.279842111416485 37.0 35.0 37.0 35.0 37.0 5 36.24734950200516 37.0 35.0 37.0 35.0 37.0 6 36.320444418876114 37.0 37.0 37.0 35.0 37.0 7 36.27817006612095 37.0 37.0 37.0 35.0 37.0 8 36.2684087362047 37.0 37.0 37.0 35.0 37.0 9 38.0003433167366 39.0 38.0 39.0 35.0 39.0 10-11 37.937777089082346 39.0 38.0 39.0 35.0 39.0 12-13 37.84069268327083 39.0 38.0 39.0 35.0 39.0 14-15 39.05528420130609 40.0 38.0 41.0 36.0 41.0 16-17 39.03351884808884 40.0 38.0 41.0 36.0 41.0 18-19 39.09374959405116 40.0 38.5 41.0 36.0 41.0 20-21 39.0406227208872 40.0 38.0 41.0 36.0 41.0 22-23 38.98984246401206 40.0 38.0 41.0 35.0 41.0 24-25 39.01683736622246 40.0 38.0 41.0 35.0 41.0 26-27 38.84694383152613 40.0 38.0 41.0 35.0 41.0 28-29 38.69345155800846 40.0 38.0 41.0 35.0 41.0 30-31 38.678055194196645 40.0 38.0 41.0 35.0 41.0 32-33 38.49928181850236 40.0 38.0 41.0 35.0 41.0 34-35 38.35595728768644 40.0 38.0 41.0 35.0 41.0 36-37 38.17276533280382 40.0 38.0 41.0 34.5 41.0 38-39 38.02885530776953 40.0 37.0 41.0 34.0 41.0 40-41 37.808798558440856 40.0 37.0 41.0 34.0 41.0 42-43 37.5642512633128 40.0 37.0 41.0 33.0 41.0 44-45 37.15483213667346 40.0 37.0 41.0 33.0 41.0 46-47 36.587387099827225 40.0 37.0 41.0 31.0 41.0 48-49 36.08839756449251 40.0 36.0 41.0 30.0 41.0 50-51 35.77680607800528 40.0 36.0 41.0 28.5 41.0 52-53 35.5139711354134 39.0 35.5 41.0 27.5 41.0 54-55 35.29398490519819 39.0 35.0 40.5 27.0 41.0 56-57 35.03158885129932 39.0 35.0 40.0 26.0 41.0 58-59 34.795852733821896 39.0 35.0 40.0 26.0 41.0 60-61 34.45111355247457 38.0 34.5 40.0 24.5 41.0 62-63 34.10240674311186 38.0 34.0 40.0 23.0 41.0 64-65 33.69580652524492 37.5 34.0 40.0 22.0 41.0 66-67 33.26544229402387 37.0 33.0 39.5 20.0 41.0 68-69 32.82654153854148 36.0 33.0 39.0 18.0 40.0 70-71 32.38235834473083 36.0 32.5 39.0 15.0 40.0 72-73 31.896927036842527 35.0 32.0 38.0 10.0 40.0 74-75 31.428174427171385 35.0 31.0 37.0 7.5 39.0 76-77 30.117014406312574 34.0 29.5 36.0 6.0 38.5 78-79 30.679018336825266 34.5 31.0 36.0 2.0 39.0 80-81 30.479495639877445 34.5 31.0 36.0 2.0 38.0 82-83 30.090386945798638 34.0 31.0 35.5 2.0 37.0 84-85 29.67914360103997 34.0 30.0 35.0 2.0 37.0 86-87 29.324154095397514 34.0 30.0 35.0 2.0 36.0 88-89 28.895338501023453 34.0 29.0 35.0 2.0 36.0 90-91 28.37373274369457 34.0 29.0 35.0 2.0 35.5 92-93 27.939396245042786 34.0 29.0 35.0 2.0 35.0 94-95 27.53230517703081 33.5 27.5 35.0 2.0 35.0 96-97 26.926080195078136 33.0 26.5 35.0 2.0 35.0 98-99 26.06516986755397 33.0 24.5 34.5 2.0 35.0 100-101 23.270940001224808 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2724244731322969 1101 2 0.2019922383034114 1101 3 0.20636518782768576 1101 4 0.1342807721003112 1101 5 0.11806683992310951 1101 6 0.20437898225563345 1101 7 0.14832770479021207 1101 8 0.1931629531684962 1101 9 0.1967469203017913 1101 10-11 0.25098529216833043 1101 12-13 0.05912875343813084 1101 14-15 0.22536281395245794 1101 16-17 0.20716449212592636 1101 18-19 0.24282334867703526 1101 20-21 0.325467633184779 1101 22-23 0.36170452008633447 1101 24-25 0.19517609981938477 1101 26-27 0.0671511909238447 1101 28-29 -0.02844214924247268 1101 30-31 0.11082181624260556 1101 32-33 0.1262238632525765 1101 34-35 -0.018961323775471328 1101 36-37 0.13186405324879047 1101 38-39 0.18301572316634918 1101 40-41 0.21594975403660754 1101 42-43 0.1759742080126756 1101 44-45 -0.01573839739135252 1101 46-47 0.06650305263314493 1101 48-49 0.015984664672267 1101 50-51 -0.15449431478510434 1101 52-53 -0.22588202438473104 1101 54-55 -0.11559226393917754 1101 56-57 -0.26801121308179887 1101 58-59 -0.09005463605031139 1101 60-61 0.2175686997575781 1101 62-63 0.1495753178932091 1101 64-65 0.09131394396736425 1101 66-67 -0.014907736114665227 1101 68-69 0.010597847642905833 1101 70-71 -0.0829955338623023 1101 72-73 -0.20262021733065438 1101 74-75 0.12816793158796713 1101 76-77 0.17749119662609658 1101 78-79 0.3512489981300213 1101 80-81 0.12412754743396803 1101 82-83 -0.04405241702794527 1101 84-85 -0.03750326899011114 1101 86-87 0.05346064561092234 1101 88-89 0.2560637691903942 1101 90-91 0.24019420294814608 1101 92-93 0.3592772945158238 1101 94-95 0.3161983951623313 1101 96-97 0.20199864971018044 1101 98-99 0.4982809161189685 1101 100-101 0.894680233953661 1102 1 0.2190927357043293 1102 2 0.20641517602320647 1102 3 0.1201748989118201 1102 4 -0.020419454176270335 1102 5 -0.06311401605213263 1102 6 -0.03091446484295801 1102 7 0.13485370379846984 1102 8 0.11869686900728738 1102 9 0.093269556509469 1102 10-11 0.10535892781174283 1102 12-13 -0.06926753266210284 1102 14-15 0.14555899631884017 1102 16-17 0.2752270060188522 1102 18-19 0.16877333984255927 1102 20-21 0.2553076436721611 1102 22-23 0.1967107042196119 1102 24-25 0.17064475452815486 1102 26-27 0.026897327977920327 1102 28-29 0.044239940709594805 1102 30-31 0.08323830194500914 1102 32-33 -0.03967619887374241 1102 34-35 0.032198842426666374 1102 36-37 0.024268683654206313 1102 38-39 0.20925411722958387 1102 40-41 0.03493988792165936 1102 42-43 0.08454307987857135 1102 44-45 -0.07466435308217712 1102 46-47 0.010392729975279735 1102 48-49 0.03909964395399612 1102 50-51 -0.11115408486073619 1102 52-53 -0.2190224811556476 1102 54-55 -0.28677021308679684 1102 56-57 0.0791267016398578 1102 58-59 -0.20262011091765686 1102 60-61 -0.13944387939648095 1102 62-63 0.07129900967828462 1102 64-65 0.06935599607367493 1102 66-67 0.17560916623303058 1102 68-69 0.04587020599205971 1102 70-71 -0.013852934018473206 1102 72-73 0.06774894977773727 1102 74-75 0.160599008425109 1102 76-77 0.08793240746229358 1102 78-79 0.07302284694078764 1102 80-81 0.023326174955354162 1102 82-83 -0.004721899327645218 1102 84-85 0.23523695751445928 1102 86-87 0.25914719839834177 1102 88-89 0.4254092770017799 1102 90-91 0.7074496447004464 1102 92-93 0.5908488623218524 1102 94-95 0.7806211048956406 1102 96-97 0.7601124035645803 1102 98-99 0.9998682719887633 1102 100-101 1.517046277647271 1103 1 0.16268529650547237 1103 2 0.2538360630046128 1103 3 0.1094876313867843 1103 4 0.03361888631014409 1103 5 0.09108569782483755 1103 6 0.00520341784660161 1103 7 0.12558560651664408 1103 8 -0.02220384630029315 1103 9 0.1740409442061761 1103 10-11 0.10631915671016401 1103 12-13 0.06914734573560821 1103 14-15 0.33341593480238885 1103 16-17 0.4005775067341517 1103 18-19 0.09101883340478878 1103 20-21 0.14548675669075806 1103 22-23 0.2163318773240519 1103 24-25 0.24015777312614972 1103 26-27 0.17978545387205003 1103 28-29 0.032980427118751265 1103 30-31 0.1367787883541567 1103 32-33 -0.19364014737159607 1103 34-35 -0.12635680850323183 1103 36-37 -0.3027069958879949 1103 38-39 -0.18102571109659493 1103 40-41 -0.24160031236446144 1103 42-43 -0.07221915188108596 1103 44-45 -0.05393760057860675 1103 46-47 0.045575562593299423 1103 48-49 0.15412224767359106 1103 50-51 -0.01832432806531159 1103 52-53 0.1916212956683907 1103 54-55 0.19914452224940504 1103 56-57 0.22064429820638054 1103 58-59 0.19985771454729928 1103 60-61 0.19020190028878403 1103 62-63 0.0671593816382341 1103 64-65 0.06667330651716696 1103 66-67 0.43975680571800524 1103 68-69 0.2353649127159514 1103 70-71 0.45544377556664983 1103 72-73 0.1579566750996264 1103 74-75 0.5595952888256797 1103 76-77 0.45719478228346233 1103 78-79 0.38762084407955655 1103 80-81 0.40513790599970534 1103 82-83 0.43259884459224196 1103 84-85 0.5404112064414974 1103 86-87 0.5204587863811732 1103 88-89 0.6302172884467012 1103 90-91 0.826276540265539 1103 92-93 1.032556444582223 1103 94-95 0.8690525784292653 1103 96-97 1.0496002433356786 1103 98-99 0.9584594021747428 1103 100-101 1.5657123720678676 1104 1 0.09080837342855119 1104 2 0.08565144761999477 1104 3 0.08524147754063449 1104 4 0.05872657861783637 1104 5 -0.06946814832900827 1104 6 -0.033011966768782486 1104 7 -0.015891316560285418 1104 8 -0.1441115386079872 1104 9 0.12862555959079458 1104 10-11 0.061365310556318775 1104 12-13 0.1626858072740731 1104 14-15 0.16464670403316006 1104 16-17 0.10134673750339118 1104 18-19 -0.04015039736444237 1104 20-21 0.3029944489984473 1104 22-23 0.09768572347789473 1104 24-25 0.1389885423569197 1104 26-27 0.16550853079512962 1104 28-29 0.06334965788797575 1104 30-31 0.05311724989262245 1104 32-33 0.1355598526284041 1104 34-35 -0.0013721931186125857 1104 36-37 0.12590838872738885 1104 38-39 0.31491275044186295 1104 40-41 0.3560304568663142 1104 42-43 0.3102116173496867 1104 44-45 0.3386777840367827 1104 46-47 0.34145248567023145 1104 48-49 0.3156914784428224 1104 50-51 0.5112141334731533 1104 52-53 0.37931360336069986 1104 54-55 0.5650829837878604 1104 56-57 0.6175981443602296 1104 58-59 0.5833961760857633 1104 60-61 0.8318326695195566 1104 62-63 0.9150978431766958 1104 64-65 0.8091656142094763 1104 66-67 0.7343414211026271 1104 68-69 0.617549528100561 1104 70-71 0.7923053140281908 1104 72-73 0.997772059715011 1104 74-75 0.8718414426718368 1104 76-77 0.8308255515142342 1104 78-79 0.7008516133103271 1104 80-81 0.6807686752304711 1104 82-83 0.32210653689993407 1104 84-85 0.5249035141338041 1104 86-87 0.6580280171504072 1104 88-89 0.753324980754396 1104 90-91 0.5185842325732324 1104 92-93 0.8530795215053004 1104 94-95 1.021883347660033 1104 96-97 1.576984858720298 1104 98-99 1.9044286329439721 1104 100-101 2.2140816028371013 1105 1 0.14628734079848726 1105 2 0.14120642075588563 1105 3 0.13421539970024554 1105 4 0.1667596018127 1105 5 0.14421551043130876 1105 6 0.20349438888577964 1105 7 0.1556012794297601 1105 8 0.1327617414339457 1105 9 0.2964095983799382 1105 10-11 0.12007452297932986 1105 12-13 0.10104513108484525 1105 14-15 0.2140701085672987 1105 16-17 0.22756217453654415 1105 18-19 0.2195626153468737 1105 20-21 0.26515012977423424 1105 22-23 0.32706850424057166 1105 24-25 0.15367600140553606 1105 26-27 0.2903571744853437 1105 28-29 0.25821926868604095 1105 30-31 0.16348679516847398 1105 32-33 0.34021061923828455 1105 34-35 0.2436520500998398 1105 36-37 0.16786881331132264 1105 38-39 0.3821306773190827 1105 40-41 0.3393550539047041 1105 42-43 0.2093794852569104 1105 44-45 0.5059533704605812 1105 46-47 0.27639941267848656 1105 48-49 0.2699478013362935 1105 50-51 0.163883508391244 1105 52-53 0.25376780333448323 1105 54-55 0.13030772497511833 1105 56-57 0.12307973261864902 1105 58-59 -0.04429220027382286 1105 60-61 0.07113682362779628 1105 62-63 0.3963191772089303 1105 64-65 0.32319391980507106 1105 66-67 0.23701472735343998 1105 68-69 0.013493703175917915 1105 70-71 0.1549340651748281 1105 72-73 0.3931907466498785 1105 74-75 0.23237348303351624 1105 76-77 0.15755730357818365 1105 78-79 -0.01485275046899659 1105 80-81 0.0892551121325802 1105 82-83 0.09250753283755841 1105 84-85 0.14787560167147973 1105 86-87 0.10761653765885981 1105 88-89 0.49241422200718077 1105 90-91 0.6494792659895623 1105 92-93 0.27378847183946675 1105 94-95 0.41900692255191885 1105 96-97 0.6576479435060385 1105 98-99 1.2993152795171632 1105 100-101 1.5549307707050062 1106 1 -0.0684988094547947 1106 2 0.25119058839092645 1106 3 0.1030169912101826 1106 4 0.08650718653089484 1106 5 0.10352896493300534 1106 6 0.07155926773622667 1106 7 0.04753880739963989 1106 8 0.10152948703304077 1106 9 -0.06583190347594581 1106 10-11 -0.03259121856577707 1106 12-13 -0.1949674445513665 1106 14-15 -0.09586441839849158 1106 16-17 -0.1803054954733483 1106 18-19 0.0632678400273079 1106 20-21 0.15755209885411858 1106 22-23 0.2481323188251494 1106 24-25 0.2638858084462399 1106 26-27 0.147879052106056 1106 28-29 0.12319852866399827 1106 30-31 0.09928298261464619 1106 32-33 0.06264594534363965 1106 34-35 -0.05574135596459939 1106 36-37 0.15983605046962168 1106 38-39 0.26666347212636765 1106 40-41 0.2131793785847691 1106 42-43 0.22343117924474143 1106 44-45 -0.09286916790752997 1106 46-47 -0.05283856544201626 1106 48-49 0.12706352802680243 1106 50-51 -0.11089827950019071 1106 52-53 -0.1226833400932037 1106 54-55 -0.11502059916340812 1106 56-57 -0.06345636404527255 1106 58-59 0.14157073882986282 1106 60-61 0.1237277281033613 1106 62-63 0.06636291364706182 1106 64-65 0.09010465309553695 1106 66-67 0.44500537083941794 1106 68-69 0.399184780265621 1106 70-71 0.3164486320787887 1106 72-73 0.45249442344399426 1106 74-75 0.24528005703760059 1106 76-77 0.36572281760355096 1106 78-79 0.24393872487425838 1106 80-81 0.36204563226901953 1106 82-83 0.23375027947082927 1106 84-85 0.18122886648564673 1106 86-87 0.1789806406858112 1106 88-89 0.20311441868952684 1106 90-91 0.11916837249512113 1106 92-93 0.19578116864403583 1106 94-95 -0.04238053415351928 1106 96-97 0.14265984598468862 1106 98-99 0.17573445736238114 1106 100-101 0.24657948023564202 1107 1 0.1864613614141959 1107 2 0.03332815225410002 1107 3 0.0649699843279592 1107 4 -0.008428983872413198 1107 5 0.11514411567473104 1107 6 0.1169989553719546 1107 7 0.08767242193246005 1107 8 0.1677904741865177 1107 9 0.13409287118386715 1107 10-11 0.06782341634505684 1107 12-13 0.24345971896075724 1107 14-15 0.22087232425472791 1107 16-17 0.2274740584582986 1107 18-19 0.27502045612284576 1107 20-21 0.29553462687331233 1107 22-23 0.33826615724291287 1107 24-25 0.1116238988868048 1107 26-27 0.10817977435886661 1107 28-29 0.303472922047753 1107 30-31 0.4195503907882383 1107 32-33 0.3223144165229357 1107 34-35 0.36973406370772466 1107 36-37 0.22628286212417237 1107 38-39 0.25666029296020554 1107 40-41 0.06093761867002456 1107 42-43 0.10112283594853011 1107 44-45 0.455969093133362 1107 46-47 0.45030254028093 1107 48-49 0.3025490225214682 1107 50-51 0.10152151371967477 1107 52-53 0.20829472366027346 1107 54-55 0.2621449279289081 1107 56-57 0.13247878096500187 1107 58-59 0.12049868817813092 1107 60-61 0.43763232625227744 1107 62-63 0.3949386919830573 1107 64-65 0.3070513998234503 1107 66-67 0.17717181585999242 1107 68-69 0.2827937159613825 1107 70-71 0.40122714270660964 1107 72-73 0.5529538353430326 1107 74-75 0.4708553293936326 1107 76-77 0.3564629364416767 1107 78-79 0.4912151645663734 1107 80-81 0.2847224901782006 1107 82-83 0.4082775464177999 1107 84-85 0.4640671902143154 1107 86-87 0.40886122045825246 1107 88-89 0.7668387894609019 1107 90-91 0.9197451001032704 1107 92-93 1.0465215561440893 1107 94-95 1.2645187041899106 1107 96-97 1.1078882757900423 1107 98-99 1.6668439863532392 1107 100-101 1.787693710810263 1108 1 0.10623823768194285 1108 2 0.11456547476931433 1108 3 0.20320527844560843 1108 4 0.15759535689837634 1108 5 0.07609746253071847 1108 6 0.23160341784659977 1108 7 0.15247972416369748 1108 8 0.07701968311147311 1108 9 0.11735859126500259 1108 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5738.0 17 9448.0 18 8567.0 19 4210.0 20 2879.0 21 2602.0 22 2649.0 23 2846.0 24 3062.0 25 3457.0 26 4192.0 27 4855.0 28 6047.0 29 7431.0 30 9530.0 31 12261.0 32 15924.0 33 22108.0 34 32258.0 35 49298.0 36 78836.0 37 125181.0 38 105338.0 39 9652.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.11669872199491 20.314414501384938 15.215203879313547 19.35368289730661 2 15.213570846656188 22.599891252103973 40.463124998840144 21.72341290239969 3 20.125041522767468 27.958044839021568 30.7606228693485 21.15629076886247 4 12.015157897862343 18.073863204054476 39.29157983227586 30.619399065807322 5 12.23531988019107 40.039862078692344 33.49361056159508 14.231207479521506 6 28.460029580912334 38.47374369271482 18.074605510511986 14.991621215860862 7 26.37210709255263 36.04584484681578 20.749321253532916 16.832726807098677 8 26.33313600353338 34.56067520195375 19.752218104483347 19.353970690029527 9 27.681906836828052 17.22652038280744 21.07148225609202 34.0200905242725 10-11 23.976127424326496 28.667968177322166 26.657709502079385 20.69819489627195 12-13 26.71685528381654 26.194894902697307 27.759384151705348 19.32886566178081 14-15 23.67465821427047 26.493103045126666 28.23371889967914 21.59851984092373 16-17 20.004880664958126 30.415357578299414 28.812439571614945 20.76732218512752 18-19 19.87646164780899 28.148075292143986 32.92908560834798 19.046377451699048 20-21 21.431355082952898 26.854563337416025 32.72696062056935 18.987120959061723 22-23 20.778182027378243 27.041514609122803 32.181268366370766 19.999034997128188 24-25 20.54481582448906 27.097526078153734 32.848359781093826 19.509298316263376 26-27 20.757043756472164 26.855539430560864 33.13692594915801 19.250490863808956 28-29 20.018669838930883 27.25545944320824 33.56367510026223 19.16219561759864 30-31 20.24592704213904 27.559064616161184 33.037770548510835 19.15723779318894 32-33 19.938295661210702 27.747930816909772 32.64985339420258 19.66392012767695 34-35 20.426974674359435 26.996201246703695 32.963510070314975 19.61331400862189 36-37 20.879410460211446 27.01058343431794 32.83527662978022 19.274729475690393 38-39 20.82642833680671 27.051874231016903 33.00693499807929 19.114762434097106 40-41 20.35153778061504 27.386004925203345 33.103342049248326 19.159115244933293 42-43 20.11523585395911 28.10147585039569 32.23170405784621 19.551584237798995 44-45 20.566157135142458 27.912207415270355 31.54561194816474 19.976023501422443 46-47 20.622108484377232 28.32428028749529 30.737611369165702 20.31599985896177 48-49 20.912443097570616 28.499557399774712 30.44514262490698 20.142856877747693 50-51 21.350032754272437 28.799913150144473 30.16167434644556 19.688379749137532 52-53 20.898896004721067 29.33622956569505 29.77808748452755 19.98678694505633 54-55 20.234346148635733 29.02446085354108 30.17206663685069 20.569126360972493 56-57 21.127990335169926 29.361375196943186 29.395335717374238 20.115298750512657 58-59 21.058120739856847 28.760199754667713 29.6112541082023 20.570425397273137 60-61 20.730578015480802 28.887505312130585 30.11036241257022 20.271554259818394 62-63 21.354486593017494 28.621852388649394 30.044482714466252 19.97917830386686 64-65 20.975539146458917 28.799541996915718 30.071391323550973 20.153527533074392 66-67 20.62507771020727 28.867555826085017 29.932394439382325 20.574972024325383 68-69 20.78114764289863 29.206604300552463 29.64001848343079 20.372229573118116 70-71 20.802767318473595 29.40164532226307 29.225533115218955 20.570054244044382 72-73 20.80191664238697 28.713726596382354 29.338101432842357 21.146255328388317 74-75 20.65263583744231 29.23889463145412 29.023718547083572 21.084750984019998 76-77 20.92348490612607 28.746095931975034 29.494897570987693 20.8355215909112 78-79 20.727979942879518 28.53333234359139 29.575808974856226 21.162878738672866 80-81 20.62535607512884 29.578963777300636 29.021584416018232 20.774095731552293 82-83 20.56046149320927 29.450626740365532 29.002086814837636 20.986824951587565 84-85 20.507070011626386 28.87333549220995 28.703161578924412 21.91643291723925 86-87 20.530990366717948 29.1046299509521 28.76669493617092 21.59768474615903 88-89 20.847862435767293 28.103815269614984 29.131816924958386 21.916505369659337 90-91 20.377706570750615 28.08810988029981 29.529541791132036 22.004641757817538 92-93 20.285179287896167 28.42328348680719 29.419471693631298 21.87206553166535 94-95 19.983075412768784 28.50234104899037 29.18711875604284 22.327464782198007 96-97 19.674275926444853 28.48062858510822 29.31080556952535 22.534289918921576 98-99 19.876424188494404 28.762135584507647 28.639933043771148 22.721507183226798 100-101 20.139722170407964 29.406709745194497 28.240007349891005 22.213560734506533 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 386.0 1 282.5 2 208.0 3 255.5 4 233.0 5 238.5 6 380.5 7 690.5 8 1102.0 9 1382.0 10 1383.0 11 1281.5 12 1336.0 13 1449.0 14 1516.5 15 1613.0 16 1651.0 17 1685.5 18 1758.0 19 1833.0 20 1997.5 21 2185.0 22 2401.5 23 2726.5 24 3090.5 25 3556.5 26 4247.5 27 5133.5 28 6001.5 29 7100.0 30 8421.0 31 9394.0 32 10600.0 33 12104.0 34 13543.5 35 14900.0 36 16658.0 37 18071.5 38 18503.0 39 18701.5 40 18773.5 41 18814.5 42 18595.5 43 18703.5 44 19452.0 45 19799.5 46 19526.0 47 20825.0 48 27614.5 49 26421.5 50 19074.5 51 16506.0 52 13784.0 53 12283.5 54 11214.5 55 9890.5 56 8545.5 57 7486.5 58 6718.0 59 5926.5 60 4979.0 61 4029.5 62 3088.0 63 2096.5 64 1341.5 65 957.5 66 664.0 67 463.5 68 334.0 69 245.0 70 202.0 71 164.0 72 130.5 73 107.0 74 75.5 75 73.0 76 56.0 77 32.5 78 24.0 79 13.0 80 10.5 81 6.5 82 1.5 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.038785512404868785 2 0.0 3 0.0 4 0.0 5 5.567298431320879E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005010568588188791 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.8557661437736264E-4 22-23 4.6394153594340657E-4 24-25 0.0 26-27 0.002783649215660439 28-29 0.004453838745056703 30-31 3.711532287547253E-4 32-33 1.8557661437736264E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009835560562000219 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.711532287547253E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.7836492156604393E-4 84-85 9.278830718868132E-5 86-87 0.0 88-89 0.0 90-91 0.010485078712320989 92-93 0.0012990363006415385 94-95 0.0 96-97 0.0 98-99 2.7836492156604393E-4 100-101 0.014382187614245603 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 538861.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.926847693048025 #Duplication Level Percentage of deduplicated Percentage of total 1 83.46211689123375 52.52007917754037 2 10.153157888373165 12.778124400902547 3 2.7849919373024217 5.257522903949888 4 1.1934735124900409 3.0040610378459145 5 0.6127960218499594 1.9280660966929062 6 0.3599450299382096 1.3590123646074797 7 0.24648064184759327 1.0857174866179753 8 0.17142386733266973 0.8629730872476944 9 0.14228400615904543 0.8058135586254265 >10 0.7811225701498904 8.977013184154016 >50 0.05472969366613669 2.398049222649613 >100 0.03182650431200503 3.702825875136822 >500 0.0032718836208602818 1.3972398098314789 >1k 0.00208210775872927 2.9275589906998207 >5k 2.9744396553275286E-4 0.9959428034980977 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5321 0.9874531651019465 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4202 0.7797929336136777 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2730 0.5066241572501999 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2561 0.4752617094204257 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1980 0.36744169646717795 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1760 0.32661484130415824 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1279 0.2373524897886468 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1129 0.2095159976320424 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 784 0.1454920656718523 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 768 0.1425228398418145 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 734 0.13621323495298415 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 709 0.1315738195935501 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 706 0.131017089750418 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 677 0.1256353679334745 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 673 0.12489306147596504 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 649 0.12043922273090833 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 638 0.11839787997275734 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 611 0.11338731138456856 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.855766143773626E-4 0.0 3 0.0 0.0 0.0 1.855766143773626E-4 0.0 4 0.0 0.0 0.0 1.855766143773626E-4 0.0 5 0.0 0.0 0.0 1.855766143773626E-4 0.0 6 0.0 0.0 0.0 1.855766143773626E-4 0.0 7 0.0 0.0 0.0 1.855766143773626E-4 0.0 8 0.0 0.0 0.0 1.855766143773626E-4 0.0 9 0.0 0.0 0.0 1.855766143773626E-4 0.0 10-11 0.0 0.0 0.0 3.711532287547252E-4 0.0 12-13 0.0 0.0 0.0 3.711532287547252E-4 0.0 14-15 0.0 0.0 0.0 3.711532287547252E-4 0.0 16-17 0.0 0.0 0.0 3.711532287547252E-4 0.0 18-19 0.0 0.0 0.0 4.6394153594340657E-4 0.0 20-21 0.0 0.0 0.0 7.423064575094504E-4 0.0 22-23 0.0 0.0 0.0 0.001206247993452857 0.0 24-25 0.0 0.0 0.0 0.0016701895293962637 0.0 26-27 0.0 0.0 0.0 0.002412495986905714 0.0 28-29 0.0 0.0 0.0 0.0038971089019246147 0.0 30-31 0.0 0.0 0.0 0.008258159339792636 0.0 32-33 0.0 0.0 0.0 0.013732669463924835 0.0 34-35 0.0 0.0 0.0 0.0183720848233589 0.0 36-37 0.0 0.0 0.0 0.025331207862509995 0.0 38-39 0.0 0.0 0.0 0.033496578895113954 0.0 40-41 0.0 0.0 0.0 0.04351771607149153 0.0 42-43 0.0 0.0 0.0 0.0610547061301523 0.0 44-45 0.0 0.0 0.0 0.08388062969856791 0.0 46-47 0.0 0.0 0.0 0.14122380354117295 0.0 48-49 0.0 0.0 0.0 0.26769426623934556 0.0 50-51 0.0 0.0 0.0 0.3586268072842533 0.0 52-53 0.0 0.0 0.0 0.45058001970823647 0.0 54-55 0.0 0.0 0.0 0.5784423070142393 0.0 56-57 0.0 0.0 0.0 0.6772618541701849 0.0 58-59 0.0 0.0 0.0 0.8062376011624519 0.0 60-61 0.0 0.0 0.0 1.0354247199184947 0.0 62-63 0.0 0.0 0.0 1.1881542735510642 0.0 64-65 0.0 0.0 0.0 1.3675140713467853 0.0 66-67 0.0 0.0 0.0 1.67677749920666 0.0 68-69 0.0 0.0 0.0 2.0095163687852713 0.0 70-71 0.0 0.0 0.0 2.3555239662918637 0.0 72-73 0.0 0.0 0.0 2.7701021228108917 0.0 74-75 0.0 0.0 0.0 3.1222337485919374 0.0 76-77 0.0 0.0 0.0 3.3999491520076606 0.0 78-79 0.0 0.0 0.0 3.752080777788706 0.0 80-81 0.0 0.0 0.0 4.167865182301187 0.0 82-83 0.0 0.0 0.0 4.585134199728687 0.0 84-85 0.0 0.0 0.0 5.023095009659263 0.0 86-87 0.0 0.0 0.0 5.8482614254882055 0.0 88-89 0.0 0.0 0.0 7.05682912662078 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAG 80 4.4778312E-4 29.701832 1 TACCTGG 490 0.0 29.082172 2 GTACCCG 70 0.008367957 27.155962 1 TATGGCG 70 0.008386925 27.14336 4 GTACATG 4915 0.0 22.625362 1 ACCTGGG 630 0.0 22.619467 3 GAGTACT 4160 0.0 22.608593 12-13 AGTACTT 4205 0.0 22.536089 12-13 TACATGG 4960 0.0 22.218153 2 TCTATAC 150 3.0289228E-5 22.167078 3 GTACTTT 4385 0.0 21.284052 14-15 CATGGGT 430 0.0 20.988762 4 ACATGGG 4965 0.0 20.856379 3 GGTATAG 140 4.831383E-4 20.366972 1 CATATAG 210 8.5887405E-7 20.357521 3 ATAGGGT 140 4.8465314E-4 20.357521 3 GTATAGC 195 1.0266915E-5 19.496586 1 ATGGGTA 245 1.7513412E-7 19.388115 5 TACTTTT 4920 0.0 19.30751 14-15 GTACCTG 740 0.0 19.266054 1 >>END_MODULE