FastQCFastQC Report
Thu 26 May 2016
SRR937302_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937302_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523260
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44790.855979818828116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32790.6266483201467722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.40285899935022745No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20050.3831747123800787No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18250.34877498757787717No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15460.29545541413446474No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9960.19034514390551543No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9830.18786071933646753No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA8860.16932308985972555No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8850.16913198027749113No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT8530.1630164736459886No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.159385391583534No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7830.14963880288957687No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6840.130718954248366No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5770.11027022894927951No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.10625692772235601No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5540.10587470855788708No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA250.00501686457.008279
GTATAGG1550.042.9423521
TACCTGG4550.033.4306532
CTGGTCG1205.368238E-627.7123559
GGCGTAA350.00838528827.14679710-11
CGCATCC705.82706E-727.1312188-89
GTACATG45100.026.5652241
CGGCGCC559.472633E-525.91037222-23
TACATGG45050.025.6399822
GTACCTG6450.025.0615941
ACCTGGG5950.024.765723
ATGGGAT13250.024.7560355
ACATGGG45450.024.5776423
GAGTACT32400.023.24025512-13
GTCCTAC1452.3148163E-522.9519441
TAGGACA2903.6379788E-1222.9497474
CCTAGGT1452.3182492E-522.9475483
CATGGGA35350.022.8616394
CCGTGTA1050.002192777822.6223329
AGTACTT32950.022.34770612-13
Produced by FastQC (version 0.11.5)