##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937302_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 523260 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07151129457631 33.0 31.0 34.0 31.0 34.0 2 32.17708978328174 33.0 31.0 34.0 31.0 34.0 3 32.20585177540802 34.0 31.0 34.0 30.0 34.0 4 35.80044337423078 37.0 35.0 37.0 35.0 37.0 5 35.808701219279136 37.0 35.0 37.0 35.0 37.0 6 35.8816630355846 37.0 36.0 37.0 35.0 37.0 7 35.84260405916753 37.0 35.0 37.0 35.0 37.0 8 35.85493253831747 37.0 36.0 37.0 35.0 37.0 9 37.51610098230325 39.0 37.0 39.0 35.0 39.0 10-11 37.42311374842335 39.0 37.0 39.0 35.0 39.0 12-13 37.310926690364255 39.0 37.0 39.0 35.0 39.0 14-15 38.48802698467301 40.0 38.0 41.0 34.0 41.0 16-17 38.47992298283836 40.0 38.0 41.0 34.5 41.0 18-19 38.51896667048886 40.0 38.0 41.0 34.0 41.0 20-21 38.51598631655391 40.0 38.0 41.0 34.0 41.0 22-23 38.38430608110691 40.0 38.0 41.0 34.0 41.0 24-25 38.240543897871035 40.0 38.0 41.0 34.0 41.0 26-27 38.06562894163513 40.0 38.0 41.0 34.0 41.0 28-29 38.108463287849254 40.0 38.0 41.0 34.0 41.0 30-31 37.913761801016705 40.0 37.5 41.0 34.0 41.0 32-33 37.695146772159156 40.0 37.0 41.0 33.0 41.0 34-35 37.55686847838551 40.0 37.0 41.0 33.0 41.0 36-37 37.545266215648056 40.0 37.0 41.0 33.0 41.0 38-39 37.445828077819826 40.0 37.0 41.0 33.0 41.0 40-41 37.24995986698773 40.0 37.0 41.0 33.0 41.0 42-43 36.978460038986356 40.0 36.5 41.0 32.0 41.0 44-45 36.67669418644651 40.0 36.0 41.0 31.0 41.0 46-47 36.18174903489661 40.0 36.0 41.0 30.0 41.0 48-49 35.75356514925659 39.5 36.0 41.0 28.0 41.0 50-51 34.86332893781294 38.5 35.0 40.0 25.5 40.5 52-53 34.90735580782021 38.5 35.0 40.0 26.0 40.5 54-55 35.230288002140426 39.0 35.0 40.5 26.0 41.0 56-57 35.08181592325039 39.0 35.0 41.0 25.5 41.0 58-59 34.88022398043038 39.0 35.0 40.5 25.0 41.0 60-61 34.60950674616825 38.5 35.0 40.0 23.5 41.0 62-63 34.29027061116844 38.0 34.0 40.0 22.5 41.0 64-65 33.92362209991209 38.0 34.0 40.0 21.5 41.0 66-67 33.5671052631579 37.0 34.0 40.0 19.5 41.0 68-69 33.12564977257959 37.0 33.5 39.5 18.0 41.0 70-71 32.663892711080535 36.0 33.0 39.0 12.5 40.5 72-73 32.26448419523754 36.0 32.5 39.0 8.0 40.0 74-75 31.80533769063181 35.0 32.0 38.0 7.0 40.0 76-77 31.35160627603868 35.0 31.5 37.0 2.0 39.0 78-79 30.87734778121775 35.0 31.0 37.0 2.0 39.0 80-81 30.39607269808508 35.0 31.0 36.0 2.0 38.5 82-83 29.9260348583878 34.5 30.0 36.0 2.0 37.0 84-85 29.501992317394794 34.0 30.0 35.0 2.0 37.0 86-87 29.06759163704468 34.0 29.0 35.0 2.0 36.5 88-89 28.668791805221115 34.0 29.0 35.0 2.0 36.0 90-91 28.249271872491686 34.0 29.0 35.0 2.0 36.0 92-93 27.839333218667583 34.0 28.5 35.0 2.0 35.0 94-95 27.388857355807822 34.0 27.0 35.0 2.0 35.0 96-97 26.82864254863739 33.5 25.5 35.0 2.0 35.0 98-99 26.137672476397967 33.0 24.5 35.0 2.0 35.0 100-101 24.35063543936093 31.0 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.20024678900920634 1101 2 -0.17705479372731858 1101 3 -0.11207762571171997 1101 4 -0.1157532628063862 1101 5 -0.1622808429062914 1101 6 -0.11468168735132878 1101 7 -0.12178668564668271 1101 8 -0.05314695166800476 1101 9 -0.19555997626918753 1101 10-11 -0.223136972943216 1101 12-13 -0.28115068438743407 1101 14-15 -0.24413900878366945 1101 16-17 -0.03361991934983166 1101 18-19 -0.06425430708976876 1101 20-21 -0.15328047944927192 1101 22-23 -0.10624752792779901 1101 24-25 -0.08997181707508872 1101 26-27 -0.09248556068587988 1101 28-29 -0.13159596639658133 1101 30-31 0.0038332075058136184 1101 32-33 -0.017567985438425637 1101 34-35 -0.11348033284255621 1101 36-37 -0.03404992757302949 1101 38-39 -0.09703301969825873 1101 40-41 -0.17506879491683947 1101 42-43 -0.009965300767127871 1101 44-45 0.38287007492011327 1101 46-47 0.49379221343345847 1101 48-49 0.696867701381521 1101 50-51 0.44353515985662995 1101 52-53 0.445912274923856 1101 54-55 0.6956281380226557 1101 56-57 0.5388818600641727 1101 58-59 0.6577290173584203 1101 60-61 0.5600964761092015 1101 62-63 0.6628688209419167 1101 64-65 0.8248012732695074 1101 66-67 0.7011890061675174 1101 68-69 0.5781349013503316 1101 70-71 0.6278338202539615 1101 72-73 0.7260569805823529 1101 74-75 0.7119484428559986 1101 76-77 0.8145636376801555 1101 78-79 0.612860515951791 1101 80-81 0.6768922754366429 1101 82-83 0.8287304023974542 1101 84-85 0.8792825828894593 1101 86-87 0.9609292770297522 1101 88-89 0.5815694167400522 1101 90-91 0.9539166094306459 1101 92-93 1.01983990145553 1101 94-95 1.02015005888493 1101 96-97 0.7696308409486186 1101 98-99 1.0859404928851824 1101 100-101 1.1046654584371893 1102 1 -0.16816944194134464 1102 2 -0.06720870475409413 1102 3 -0.09714434056970589 1102 4 0.018857140494716873 1102 5 0.15451533793163463 1102 6 -0.08437838323398239 1102 7 0.0657543522738564 1102 8 -0.12608903172583297 1102 9 0.023499690608318247 1102 10-11 0.03894624744171438 1102 12-13 0.020476525636361487 1102 14-15 0.10764181107115434 1102 16-17 0.20960242335068813 1102 18-19 0.24531392880673764 1102 20-21 0.1019038523479665 1102 22-23 0.06949567257820632 1102 24-25 0.11979972595073463 1102 26-27 0.07988349446306131 1102 28-29 0.2256191777255836 1102 30-31 0.16933198766303548 1102 32-33 0.25552469249296195 1102 34-35 0.04424232611350476 1102 36-37 0.33168227928820926 1102 38-39 0.48135693843553184 1102 40-41 0.505219449413147 1102 42-43 0.7313700505777163 1102 44-45 0.6216136368610137 1102 46-47 0.5625461666276408 1102 48-49 0.6216696651777909 1102 50-51 0.6100421927773283 1102 52-53 0.8600767579213624 1102 54-55 0.6916034098518153 1102 56-57 0.7576599089181286 1102 58-59 0.9816002862961 1102 60-61 0.8996570315141597 1102 62-63 0.9294436229057155 1102 64-65 1.0032800841486988 1102 66-67 0.7230603353443499 1102 68-69 0.7258051522463944 1102 70-71 0.805061915793253 1102 72-73 0.823584163498527 1102 74-75 1.0799192871076748 1102 76-77 1.1768102567578929 1102 78-79 1.0126474199607038 1102 80-81 1.0072445238628944 1102 82-83 1.1209008189511742 1102 84-85 1.2581619841123128 1102 86-87 1.2433341744726008 1102 88-89 1.0928823290017284 1102 90-91 1.4141700656518417 1102 92-93 1.6519021887360346 1102 94-95 1.397400744105255 1102 96-97 1.6272337745951972 1102 98-99 1.711131752184599 1102 100-101 2.1540224055170967 1103 1 0.11105617235500631 1103 2 0.03786285973668413 1103 3 0.05271914912997033 1103 4 -0.00525140587741646 1103 5 0.008623056968616538 1103 6 0.11052523222063826 1103 7 0.12345270587690749 1103 8 0.14684883540414262 1103 9 0.08224048650473748 1103 10-11 0.12469126274741171 1103 12-13 0.15236202245112196 1103 14-15 0.18973827835004897 1103 16-17 0.005689128074777727 1103 18-19 -0.09606110221832154 1103 20-21 -0.10976984666750411 1103 22-23 -0.15394273231961364 1103 24-25 -0.06316544323930628 1103 26-27 0.035132936011834204 1103 28-29 0.031087863090021983 1103 30-31 -0.07052902003118078 1103 32-33 0.15538404551687535 1103 34-35 0.2620713986707983 1103 36-37 0.2672659642389803 1103 38-39 -0.12652756557704237 1103 40-41 0.17114151444170034 1103 42-43 0.3309067428917629 1103 44-45 0.6345366119582394 1103 46-47 0.6258869459773564 1103 48-49 0.687095329114257 1103 50-51 0.8553553391329132 1103 52-53 0.7592576961194197 1103 54-55 0.7842235802828554 1103 56-57 0.4972727160662984 1103 58-59 0.5395385670936506 1103 60-61 0.45575035491946636 1103 62-63 0.6087602440689537 1103 64-65 0.5120829301816272 1103 66-67 0.6674385929765165 1103 68-69 0.5228515657485033 1103 70-71 0.5966727373370588 1103 72-73 0.8043072544226639 1103 74-75 0.6861491208133081 1103 76-77 0.748010720065583 1103 78-79 0.8230718428944392 1103 80-81 0.7619148308042369 1103 82-83 0.8048836103799779 1103 84-85 0.7243157029838265 1103 86-87 0.8535765836725666 1103 88-89 1.175806131434097 1103 90-91 1.0238747070020544 1103 92-93 1.1761265620296548 1103 94-95 1.2795303047902848 1103 96-97 1.3717692259065224 1103 98-99 1.5003929418925495 1103 100-101 2.0757647733503077 1104 1 0.07157373793930333 1104 2 0.14390418242976466 1104 3 0.11866270688695835 1104 4 0.0738567852801637 1104 5 0.12524921119108967 1104 6 -0.013280415061210249 1104 7 -0.02158757725157301 1104 8 -0.009374411094974278 1104 9 0.03278855108003853 1104 10-11 0.12353704028058843 1104 12-13 -0.038454456176651775 1104 14-15 0.23249706114219748 1104 16-17 0.39768142306651555 1104 18-19 0.24393480831796666 1104 20-21 0.3070668958262246 1104 22-23 0.11162589423208402 1104 24-25 0.21178697374839572 1104 26-27 0.037683117935635835 1104 28-29 0.02605342751245132 1104 30-31 0.04723320249679119 1104 32-33 0.10134292920793797 1104 34-35 0.07566638124279734 1104 36-37 0.3330107817036634 1104 38-39 0.14516856815988177 1104 40-41 0.3574146753296077 1104 42-43 0.4595767499241177 1104 44-45 0.4422018721551382 1104 46-47 0.5490604462525326 1104 48-49 0.5136284958115027 1104 50-51 0.44547928428059436 1104 52-53 0.42680913927969755 1104 54-55 0.8299949983712835 1104 56-57 0.9556414022400901 1104 58-59 0.8428152976629733 1104 60-61 0.7953793603007426 1104 62-63 0.7174702284754844 1104 64-65 0.7128841975331781 1104 66-67 0.994589001158495 1104 68-69 0.8903280991062914 1104 70-71 1.0376057847901237 1104 72-73 1.125378885010317 1104 74-75 1.0224855346211719 1104 76-77 0.8283572161213506 1104 78-79 0.9823067692076499 1104 80-81 1.1459584695861125 1104 82-83 1.1633107720628537 1104 84-85 1.260089911080204 1104 86-87 1.31017667234131 1104 88-89 1.104388616299257 1104 90-91 1.185229848260537 1104 92-93 1.5216556698386192 1104 94-95 1.2194400308344377 1104 96-97 1.4182859200910762 1104 98-99 1.4045389701442517 1104 100-101 1.5969296589981994 1105 1 0.06657492309438595 1105 2 0.06769447770268755 1105 3 0.11397287890375196 1105 4 0.006933617191315022 1105 5 0.10658301858645558 1105 6 0.16126330023067936 1105 7 0.26486863973339325 1105 8 0.2530570192179056 1105 9 0.11179606834944167 1105 10-11 0.23638073525552983 1105 12-13 0.062108069219526385 1105 14-15 0.2726378406356318 1105 16-17 0.1493067786130382 1105 18-19 0.23930635443743142 1105 20-21 0.3215300205722613 1105 22-23 0.19791788258370246 1105 24-25 0.17750847923397117 1105 26-27 0.4305218183284367 1105 28-29 0.2666123974433745 1105 30-31 0.09022258689338258 1105 32-33 0.10952261564976595 1105 34-35 0.34455690677442874 1105 36-37 0.3289304620504083 1105 38-39 0.25896673624873756 1105 40-41 0.31876168873878896 1105 42-43 0.47783025459701634 1105 44-45 0.5270928378872242 1105 46-47 0.43131105576085815 1105 48-49 0.5242504482698536 1105 50-51 0.7428410444052105 1105 52-53 0.8188880706084305 1105 54-55 0.6585877113605818 1105 56-57 0.6146997965242562 1105 58-59 0.6898435212848 1105 60-61 0.7459859057429767 1105 62-63 0.5038862348414526 1105 64-65 0.7015850135971178 1105 66-67 0.6882793201345265 1105 68-69 0.6776223900449381 1105 70-71 0.792103025071853 1105 72-73 0.9005189872722745 1105 74-75 0.7871454797654245 1105 76-77 0.9006389782776516 1105 78-79 0.7270539925585915 1105 80-81 0.6946704599694264 1105 82-83 0.8711848578003547 1105 84-85 0.8888168005560253 1105 86-87 0.9118333498436826 1105 88-89 0.6313741743379957 1105 90-91 0.6182199098886016 1105 92-93 0.9025221875143608 1105 94-95 0.8938059728634222 1105 96-97 0.471033786811919 1105 98-99 1.0099161880773408 1105 100-101 1.4302608759831656 1106 1 0.12320657749297936 1106 2 0.13918842636974205 1106 3 0.14843377268149283 1106 4 0.16758754711163704 1106 5 0.061566063826717254 1106 6 0.0324126399599578 1106 7 0.06302429269840815 1106 8 0.005335573514493319 1106 9 0.20301267574337345 1106 10-11 -0.07495276349008861 1106 12-13 -0.21465563932401466 1106 14-15 -0.03515997040840091 1106 16-17 -0.059898415332867216 1106 18-19 -0.06286468187057181 1106 20-21 0.09482420633026578 1106 22-23 0.21997285460145832 1106 24-25 0.06612397836556028 1106 26-27 0.07268816795487254 1106 28-29 0.2867678849961024 1106 30-31 0.17214302358183176 1106 32-33 0.3019357052756604 1106 34-35 0.20439618116584768 1106 36-37 0.07907657624002695 1106 38-39 0.19787032845230357 1106 40-41 0.06723576036230838 1106 42-43 -0.05184471025287252 1106 44-45 0.01521072171266269 1106 46-47 0.3212829356715332 1106 48-49 0.4947450310173451 1106 50-51 0.7202857002098 1106 52-53 0.38241466063406904 1106 54-55 0.38624740314234884 1106 56-57 0.34651409365986297 1106 58-59 0.24216480708967225 1106 60-61 0.2404459724592627 1106 62-63 0.35785475136889744 1106 64-65 0.44030940915303773 1106 66-67 0.5706050169337971 1106 68-69 0.705254513111683 1106 70-71 0.929777052585429 1106 72-73 0.9517003067109719 1106 74-75 0.8372736742902767 1106 76-77 1.0912876701421261 1106 78-79 1.1716560381572485 1106 80-81 1.0609029193744917 1106 82-83 1.0975102237750498 1106 84-85 0.8548535220844364 1106 86-87 0.9362371763813293 1106 88-89 0.9387612115281918 1106 90-91 0.7185948444129018 1106 92-93 0.8708043808813279 1106 94-95 1.1803841439548677 1106 96-97 1.6205127380980038 1106 98-99 1.6023458189322426 1106 100-101 1.5381510422225162 1107 1 0.07669330315669498 1107 2 0.007719399821070283 1107 3 0.11209748949565324 1107 4 0.13769374180190397 1107 5 0.09809703727804475 1107 6 0.05768697624314001 1107 7 0.11146677057451626 1107 8 0.14163645847024497 1107 9 0.048375507601782886 1107 10-11 0.2537109533240667 1107 12-13 0.3007248916494305 1107 14-15 0.2815568437508915 1107 16-17 0.2879422926317403 1107 18-19 0.1224185039701382 1107 20-21 -0.07209614765204009 1107 22-23 0.28739763336252366 1107 24-25 0.46943371287883195 1107 26-27 0.3812102918486815 1107 28-29 0.15287407968636302 1107 30-31 0.12739081119244844 1107 32-33 0.2964135813818487 1107 34-35 0.23130768559062176 1107 36-37 -0.05920660960067892 1107 38-39 0.24620661163815072 1107 40-41 0.18902337098177924 1107 42-43 0.09695174992411637 1107 44-45 0.26920187215513636 1107 46-47 0.13011479407862225 1107 48-49 0.15223175668106137 1107 50-51 0.1366042842805939 1107 52-53 0.13897218275796774 1107 54-55 0.28073412880606696 1107 56-57 0.43764683702269735 1107 58-59 0.5100055150542673 1107 60-61 0.27542827334421816 1107 62-63 0.15118218499721792 1107 64-65 0.22467224101144012 1107 66-67 0.29474660985415113 1107 68-69 0.4293607078019477 1107 70-71 0.6467416543553455 1107 72-73 0.32265605892336424 1107 74-75 0.25189314331682766 1107 76-77 0.32441156394743587 1107 78-79 0.6229480735554773 1107 80-81 0.3891595565426371 1107 82-83 0.3989792503237233 1107 84-85 0.3515083893410704 1107 86-87 0.4268831940804425 1107 88-89 0.45127448586447727 1107 90-91 0.7181700656518402 1107 92-93 1.002503495925577 1107 94-95 1.1674726395300894 1107 96-97 0.9672207026997732 1107 98-99 0.48145201362250845 1107 100-101 0.7988590068242871 1108 1 0.04017845167154377 1108 2 0.0028877166527507825 1108 3 0.03845392513921553 1108 4 0.08923829625734925 1108 5 0.024275255099823312 1108 6 0.12390479802532184 1108 7 0.12633805770322937 1108 8 0.02328002282668251 1108 9 -0.0100601359625756 1108 10-11 0.039819864215154155 1108 12-13 0.1875961787781435 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1559.0 13 2223.0 14 2642.0 15 3641.0 16 5338.0 17 7872.0 18 6412.0 19 3360.0 20 3051.0 21 2992.0 22 2968.0 23 3374.0 24 3682.0 25 4155.0 26 4667.0 27 5427.0 28 6298.0 29 7632.0 30 9651.0 31 12157.0 32 15672.0 33 21388.0 34 30238.0 35 45360.0 36 70005.0 37 110115.0 38 108473.0 39 17140.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68037387477189 19.581652839331994 15.018440706037511 20.71953257985861 2 14.371226506379612 21.099182405723734 41.994876383654244 22.534714704242408 3 19.191423726540272 27.578390703288914 30.79066113564729 22.439524434523523 4 11.41482426792674 16.929565374017482 40.08572950558948 31.569880852466305 5 11.92109070353444 39.13924099144415 34.63060370019066 14.309064604830752 6 28.010968998408654 37.72106036204589 19.276942315094438 14.991028324451023 7 25.919500944918976 35.11747822473945 21.621507772844158 17.34151305749741 8 25.454016253437196 33.82066836707339 21.026639705262838 19.698675674226582 9 26.825919876845543 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20.635403891427583 34-35 19.966296438146305 26.87686710072769 32.669954040597474 20.48688242052853 36-37 20.206590208504853 26.710352486166688 32.79174405024029 20.29131325508817 38-39 20.325123086687682 26.886268187775286 32.80218344248312 19.98642528305391 40-41 19.939633435380205 27.173761126076712 33.02795854561189 19.858646892931194 42-43 19.525614354875923 27.66786877806828 32.20216150288498 20.604355364170825 44-45 19.800943202945643 27.802467339252445 31.591362972295567 20.805226485506346 46-47 19.986022087017297 27.76042240104164 30.930440735085043 21.323114776856023 48-49 20.14499386284561 27.886042330088905 30.761122036501227 21.207841770564254 50-51 20.558244897802865 28.29186980349504 30.62936353226381 20.520521766438286 52-53 20.17043142189294 28.403194128839566 30.487862099740397 20.938512349527095 54-55 19.75037961982487 28.195269161466243 30.80904500481586 21.245306213893034 56-57 20.291926363642453 28.821889116610684 29.871143075124902 21.015041444621964 58-59 20.225540876890676 28.157763737315715 30.248324717595253 21.368370668198352 60-61 20.321700265259608 27.88710336700691 30.87034060635417 20.92085576137931 62-63 20.794968264453313 27.862948390246707 30.571144107144566 20.770939238155414 64-65 20.345263633500565 27.983795545115065 30.9018035688474 20.76913725253697 66-67 20.066949820181247 28.064457150296317 30.519411523141915 21.34918150638053 68-69 19.948405500222556 28.453265816968752 30.547772773611182 21.05055590919751 70-71 19.918187057357134 28.30511460130311 30.073675685300667 21.70302265603909 72-73 19.975760848025686 28.01756859861898 30.003800530542573 22.002870022812758 74-75 19.84881759505862 28.278538646672395 30.166499771196843 21.706143987072142 76-77 20.118114909781575 27.835025886101157 30.358920748705692 21.687938455411572 78-79 19.9939115917167 27.678038077441492 30.549641588040682 21.77840874280113 80-81 19.851042035631206 28.50429355057965 30.053513818818868 21.591150594970276 82-83 19.766956535057993 28.403847552967033 30.02837417146271 21.800821740512266 84-85 19.879177850357433 27.885200770382397 29.808859397554837 22.426761981705333 86-87 20.06497615084269 27.8787698946387 30.085259660512055 21.970994294006555 88-89 20.436891391683154 26.92670328965568 30.25215875577559 22.384246562885576 90-91 19.92138926195698 27.083084045140865 30.64708354353314 22.348443149369018 92-93 19.87052283846561 27.365092857696695 30.654861902833737 22.10952240100396 94-95 19.222691689911382 27.433324207749344 30.644253466605146 22.699730635734127 96-97 19.043566824352396 27.455982325925106 30.401916733512397 23.098534116210107 98-99 19.206616032333475 27.824153227890875 29.992801168661344 22.976429571114306 100-101 19.528038112267314 27.854232255483137 30.05332616064087 22.564403471608678 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 711.0 1 427.5 2 182.0 3 242.5 4 211.5 5 169.5 6 270.5 7 598.5 8 1074.0 9 1348.5 10 1254.0 11 1091.5 12 1139.5 13 1216.5 14 1246.5 15 1319.0 16 1380.0 17 1458.5 18 1578.5 19 1630.5 20 1743.0 21 1950.5 22 2169.0 23 2527.0 24 2902.0 25 3425.0 26 4178.5 27 5132.5 28 5973.0 29 6957.0 30 8236.5 31 9200.0 32 10394.5 33 11905.5 34 13610.0 35 15041.5 36 16424.5 37 17933.5 38 18338.0 39 18248.5 40 18123.0 41 18087.0 42 18179.5 43 18436.5 44 19144.5 45 19438.5 46 19365.0 47 20561.5 48 26917.0 49 26186.0 50 18876.0 51 15823.0 52 13209.0 53 11750.0 54 10591.0 55 9369.0 56 8148.5 57 6967.5 58 6160.5 59 5442.0 60 4649.5 61 3796.0 62 2891.0 63 1964.0 64 1293.0 65 969.0 66 670.0 67 457.0 68 329.0 69 235.5 70 192.0 71 139.5 72 90.0 73 81.5 74 67.5 75 56.0 76 38.0 77 18.5 78 13.0 79 8.0 80 4.0 81 6.0 82 5.0 83 2.0 84 1.5 85 1.0 86 1.0 87 1.5 88 2.0 89 2.5 90 1.0 91 1.0 92 1.5 93 0.5 94 0.5 95 1.5 96 1.5 97 3.0 98 4.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30348201658831175 2 0.26048236058555974 3 0.25895348392768414 4 0.2648778809769522 5 0.2665978672170623 6 0.20276726675075488 7 0.18977181515881206 8 0.19722508886595574 9 0.18900737682987426 10-11 0.21127164316018807 12-13 0.20123839009287925 14-15 0.19741619844819022 16-17 0.19732064365707297 18-19 0.19158735619003936 20-21 0.18680961663417803 22-23 0.18900737682987426 24-25 0.18184076749608225 26-27 0.18012078125597217 28-29 0.19005847953216373 30-31 0.20047395176394145 32-33 0.19292512326568054 34-35 0.20257615716852045 36-37 0.18537629476741965 38-39 0.1845163016473646 40-41 0.18174521270496502 42-43 0.1881473837098192 44-45 0.19053625348774986 46-47 0.19254290410121164 48-49 0.19703397928372127 50-51 0.19760730803042464 52-53 0.21299162940029812 54-55 0.1962695409547835 56-57 0.17897412376256544 58-59 0.1834651989450751 60-61 0.17992967167373772 62-63 0.1863318426785919 64-65 0.17859190459809654 66-67 0.1774452471046898 68-69 0.17534304170011084 70-71 0.18556740434965407 72-73 0.18432519206513015 74-75 0.1867140618430608 76-77 0.18575851393188852 78-79 0.1827007606161373 80-81 0.18442074685624738 82-83 0.1823185414516684 84-85 0.1762030348201659 86-87 0.17476971295340749 88-89 0.17400527462446966 90-91 0.17954745250926882 92-93 0.1774452471046898 94-95 0.1756297060734625 96-97 0.17448304858005578 98-99 0.18212743186943392 100-101 0.17362305546000076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 523260.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.88426488430477 #Duplication Level Percentage of deduplicated Percentage of total 1 84.82770276516192 55.88810838506999 2 9.554827367765807 12.590255544433738 3 2.441571420278964 4.825834145628224 4 1.013842733800467 2.671851328989506 5 0.5293535404007914 1.7438034436605132 6 0.3302817361308483 1.3056221633815717 7 0.2129566482152828 0.9821344553922581 8 0.1650204352856567 0.8697800055746784 9 0.1323929030277802 0.7850348182695911 >10 0.7161903695654166 8.60146173357471 >50 0.047957523373249517 2.1500913149104357 >100 0.02238017592213446 2.8262554198004155 >500 0.0037784712595810717 1.841020367637436 >1k 0.0017439098121143407 2.918746873676908 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4479 0.855979818828116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3279 0.6266483201467722 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2108 0.40285899935022745 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2005 0.3831747123800787 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1825 0.34877498757787717 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1546 0.29545541413446474 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 996 0.19034514390551543 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 983 0.18786071933646753 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 886 0.16932308985972555 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 885 0.16913198027749113 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 853 0.1630164736459886 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 834 0.159385391583534 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 783 0.14963880288957687 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 684 0.130718954248366 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 577 0.11027022894927951 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 556 0.10625692772235601 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 554 0.10587470855788708 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.9110958223445324E-4 0.0 10-11 0.0 0.0 0.0 1.9110958223445324E-4 0.0 12-13 0.0 0.0 0.0 3.822191644689065E-4 0.0 14-15 0.0 0.0 0.0 3.822191644689065E-4 0.0 16-17 0.0 0.0 0.0 3.822191644689065E-4 0.0 18-19 0.0 0.0 0.0 3.822191644689065E-4 0.0 20-21 0.0 0.0 0.0 3.822191644689065E-4 0.0 22-23 0.0 0.0 0.0 5.733287467033597E-4 0.0 24-25 0.0 0.0 0.0 0.001528876657875626 0.0 26-27 0.0 0.0 0.0 0.0020066506134617593 0.0 28-29 0.0 0.0 0.0 0.003917746435806291 0.0 30-31 0.0 0.0 0.0 0.007835492871612584 0.0 32-33 0.0 0.0 0.0 0.011371020142949967 0.0 34-35 0.0 0.0 0.0 0.017008752818866338 0.0 36-37 0.0 0.0 0.0 0.024175362152658336 0.0 38-39 0.0 0.0 0.0 0.03143752627756756 0.0 40-41 0.0 0.0 0.0 0.03994190268700073 0.0 42-43 0.0 0.0 0.0 0.05484845010128808 0.0 44-45 0.0 0.0 0.0 0.07778159996942247 0.0 46-47 0.0 0.0 0.0 0.1346367006841723 0.0 48-49 0.0 0.0 0.0 0.2543668539540573 0.0 50-51 0.0 0.0 0.0 0.3290907006077285 0.0 52-53 0.0 0.0 0.0 0.4053434239192753 0.0 54-55 0.0 0.0 0.0 0.5156136528685549 0.0 56-57 0.0 0.0 0.0 0.5963574513626113 0.0 58-59 0.0 0.0 0.0 0.7135076252723311 0.0 60-61 0.0 0.0 0.0 0.9403546993846271 0.0 62-63 0.0 0.0 0.0 1.0727936398731033 0.0 64-65 0.0 0.0 0.0 1.2453655926308145 0.0 66-67 0.0 0.0 0.0 1.5542942323128082 0.0 68-69 0.0 0.0 0.0 1.8486985437449834 0.0 70-71 0.0 0.0 0.0 2.16785154607652 0.0 72-73 0.0 0.0 0.0 2.554466230936819 0.0 74-75 0.0 0.0 0.0 2.8853724725757752 0.0 76-77 0.0 0.0 0.0 3.128368306386882 0.0 78-79 0.0 0.0 0.0 3.4716966708710775 0.0 80-81 0.0 0.0 0.0 3.8469403355884264 0.0 82-83 0.0 0.0 0.0 4.254672629285633 0.0 84-85 0.0 0.0 0.0 4.675591484157016 0.0 86-87 0.0 0.0 0.0 5.481596147230822 0.0 88-89 0.0 0.0 0.0 6.685586515307877 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCGTA 25 0.005016864 57.00827 9 GTATAGG 155 0.0 42.942352 1 TACCTGG 455 0.0 33.430653 2 CTGGTCG 120 5.368238E-6 27.712355 9 GGCGTAA 35 0.008385288 27.146797 10-11 CGCATCC 70 5.82706E-7 27.13121 88-89 GTACATG 4510 0.0 26.565224 1 CGGCGCC 55 9.472633E-5 25.910372 22-23 TACATGG 4505 0.0 25.639982 2 GTACCTG 645 0.0 25.061594 1 ACCTGGG 595 0.0 24.76572 3 ATGGGAT 1325 0.0 24.756035 5 ACATGGG 4545 0.0 24.577642 3 GAGTACT 3240 0.0 23.240255 12-13 GTCCTAC 145 2.3148163E-5 22.951944 1 TAGGACA 290 3.6379788E-12 22.949747 4 CCTAGGT 145 2.3182492E-5 22.947548 3 CATGGGA 3535 0.0 22.861639 4 CCGTGTA 105 0.0021927778 22.622332 9 AGTACTT 3295 0.0 22.347706 12-13 >>END_MODULE