FastQCFastQC Report
Thu 26 May 2016
SRR937302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937302_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523260
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55571.0619959484768566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43180.825211176088369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.5603332951114169No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26620.5087337079081146No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18770.35871268585406874No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17600.3363528647326377No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13370.255513511447464No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.226847074112296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7590.14505217291595002No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG7530.1439055154225433No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.1433321866758399No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7060.13492336505752398No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7030.13435003631082063No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG6920.13224783090624165No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG6850.13091006383060047No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6660.12727898176814587No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA6520.12460344761686351No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA5580.1066391468868249No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5530.10568359897565265No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC250.005020430356.998053
CGGGTCG600.00394966431.6655856
TAAGGTG2000.030.8739455
GGTAAGG1401.702756E-830.5346683
CCTATAC1354.218964E-728.1471843
TACCTGG5300.026.8858722
GAGTACT41450.025.15534212-13
CTACACT1701.1207521E-725.14624
CCCTATA950.001225906424.9991462
GTAAGGT1901.1883458E-824.9991464
GTACTTT43100.023.91449514-15
GTCTAGG1452.319784E-522.9455971
AGTACTT42250.022.71145412-13
GTACATG47150.021.6733531
ACCTGGG6850.021.4956173
TACATGG47700.021.2099672
ACATGGG46950.020.9417763
GGTGTGC2751.2532837E-920.7285448
TCCCGCT1150.003719284720.653448
AAGGGTA1404.8479988E-420.3564454
Produced by FastQC (version 0.11.5)