##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937302_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 523260 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.463796200741506 33.0 31.0 34.0 31.0 34.0 2 32.667977678400796 34.0 31.0 34.0 31.0 34.0 3 32.72695409547835 34.0 31.0 34.0 31.0 34.0 4 36.26862936207621 37.0 35.0 37.0 35.0 37.0 5 36.192969078469595 37.0 35.0 37.0 35.0 37.0 6 36.30313228605282 37.0 37.0 37.0 35.0 37.0 7 36.27570806100218 37.0 37.0 37.0 35.0 37.0 8 36.27152658334289 37.0 37.0 37.0 35.0 37.0 9 38.00842793257654 39.0 38.0 39.0 35.0 39.0 10-11 37.946656537858814 39.0 38.0 39.0 35.0 39.0 12-13 37.872427665023125 39.0 38.0 39.0 35.0 39.0 14-15 39.07503917746436 40.0 38.0 41.0 36.0 41.0 16-17 39.07375874326338 40.0 38.0 41.0 36.0 41.0 18-19 39.16297538508581 40.0 38.5 41.0 36.0 41.0 20-21 39.13188567824791 40.0 39.0 41.0 36.0 41.0 22-23 39.021243741161186 40.0 38.0 41.0 35.5 41.0 24-25 38.87691682910981 40.0 38.0 41.0 35.0 41.0 26-27 38.644324045407636 40.0 38.0 41.0 35.0 41.0 28-29 38.606883767152084 40.0 38.0 41.0 35.0 41.0 30-31 38.65706627680312 40.0 38.0 41.0 35.0 41.0 32-33 38.544026870007265 40.0 38.0 41.0 35.0 41.0 34-35 38.403953101708524 40.0 38.0 41.0 35.0 41.0 36-37 38.23819802774911 40.0 38.0 41.0 35.0 41.0 38-39 38.05984118793717 40.0 37.5 41.0 34.0 41.0 40-41 37.869330734243015 40.0 37.0 41.0 34.0 41.0 42-43 37.59990157856515 40.0 37.0 41.0 33.0 41.0 44-45 37.19169724419983 40.0 37.0 41.0 33.0 41.0 46-47 36.6303548904942 40.0 37.0 41.0 31.5 41.0 48-49 36.10913886022245 40.0 36.0 41.0 29.5 41.0 50-51 35.8134455146581 40.0 36.0 41.0 28.5 41.0 52-53 35.55613557313764 39.5 36.0 41.0 27.0 41.0 54-55 35.35722203111264 39.0 35.0 41.0 27.0 41.0 56-57 35.14920498413791 39.0 35.0 41.0 26.0 41.0 58-59 34.92395845277682 39.0 35.0 40.0 26.0 41.0 60-61 34.6048322057868 38.5 35.0 40.0 24.5 41.0 62-63 34.24093853915835 38.0 34.5 40.0 22.5 41.0 64-65 33.91191186026067 38.0 34.0 40.0 22.0 41.0 66-67 33.533946795092305 37.0 34.0 40.0 20.5 41.0 68-69 33.107133165156895 36.5 33.5 39.0 18.5 41.0 70-71 32.70845468791805 36.0 33.0 39.0 15.5 40.0 72-73 32.26134522034935 35.5 33.0 38.5 10.0 40.0 74-75 31.823814165042236 35.0 32.0 37.5 7.5 39.5 76-77 30.515351832740894 34.0 30.5 36.0 4.5 39.0 78-79 31.03998299124718 35.0 32.0 36.5 2.0 39.0 80-81 30.851431410770935 35.0 32.0 36.0 2.0 38.5 82-83 30.509102549401828 35.0 31.5 36.0 2.0 37.0 84-85 30.194564843481253 34.5 31.0 35.0 2.0 37.0 86-87 29.88314126820319 34.0 31.0 35.0 2.0 36.5 88-89 29.524614914191798 34.0 31.0 35.0 2.0 36.0 90-91 29.101609142682413 34.0 30.0 35.0 2.0 36.0 92-93 28.768897871039254 34.0 30.0 35.0 2.0 35.0 94-95 28.446635515804765 34.0 29.0 35.0 2.0 35.0 96-97 28.088730267935635 34.0 29.0 35.0 2.0 35.0 98-99 27.557340519053625 34.0 29.0 35.0 2.0 35.0 100-101 26.130174291939 32.5 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.4860885123368917E-4 1101 2 0.17510365030984332 1101 3 0.0531749720569934 1101 4 -0.007468913021320134 1101 5 -0.03399933446436165 1101 6 -0.048629633060016886 1101 7 -0.05647415490755492 1101 8 0.015194279533588428 1101 9 -0.04431458820086931 1101 10-11 -0.034625591073066175 1101 12-13 -0.05632362692715276 1101 14-15 0.0980415956849825 1101 16-17 -0.031180491675364408 1101 18-19 -0.06984205897672524 1101 20-21 -0.07803828143568126 1101 22-23 -0.06583424134712601 1101 24-25 -0.10269350778382602 1101 26-27 -0.16196506588985216 1101 28-29 -0.27393796986763874 1101 30-31 -0.062276180525337566 1101 32-33 -0.18064362946278578 1101 34-35 0.04731869692590607 1101 36-37 0.10433372286667719 1101 38-39 0.07988364484064192 1101 40-41 0.05427508352745036 1101 42-43 -0.022229243973477253 1101 44-45 -0.009087996695370748 1101 46-47 0.012701568266251684 1101 48-49 0.0279134529034053 1101 50-51 0.020667880808488803 1101 52-53 0.24168094230406467 1101 54-55 0.2609581719223186 1101 56-57 0.23747539106967253 1101 58-59 0.32718560619282755 1101 60-61 0.3613698976786992 1101 62-63 0.3608965619109057 1101 64-65 0.4179577528621863 1101 66-67 0.4372748749408757 1101 68-69 0.4108897540947041 1101 70-71 0.3349037147820795 1101 72-73 0.418917390730126 1101 74-75 0.4819997526487505 1101 76-77 0.4207223914578222 1101 78-79 0.38789544895551487 1101 80-81 0.26141280351187035 1101 82-83 0.4712348101225672 1101 84-85 0.3847266115637318 1101 86-87 0.19918683832365858 1101 88-89 0.2695883370307044 1101 90-91 0.5868656215768446 1101 92-93 0.5125444851777914 1101 94-95 0.47748272733910824 1101 96-97 0.5159393965423646 1101 98-99 0.5203582369462474 1101 100-101 0.6020651973395239 1102 1 0.0646752797346366 1102 2 0.06436451594642278 1102 3 0.03710617894834911 1102 4 0.06522919848134734 1102 5 0.018667753876350446 1102 6 0.08313597640957937 1102 7 0.0872323599564524 1102 8 -0.0016606344750229596 1102 9 0.050798510802174235 1102 10-11 0.13736696939198367 1102 12-13 0.04017120756577697 1102 14-15 0.08404201735903172 1102 16-17 0.1440047437091181 1102 18-19 -0.006774681488089129 1102 20-21 -0.045203427063825075 1102 22-23 -0.02556813490455312 1102 24-25 -0.01747770103101942 1102 26-27 -0.16021662457783492 1102 28-29 -0.18642705152180383 1102 30-31 -0.16523316977264102 1102 32-33 -0.14989606161844193 1102 34-35 -0.08120981125156135 1102 36-37 -0.006628296349610707 1102 38-39 -0.0754723685324521 1102 40-41 -0.1929207840668994 1102 42-43 -0.11652534951247162 1102 44-45 0.009035704397973632 1102 46-47 -0.12425514990488296 1102 48-49 0.2617324643789658 1102 50-51 0.022132445128839606 1102 52-53 0.11344459400130802 1102 54-55 0.09373354013412438 1102 56-57 0.06415305138111194 1102 58-59 0.01586290506936905 1102 60-61 0.18477054826151829 1102 62-63 0.14402056419999099 1102 64-65 0.10886390026738013 1102 66-67 0.06389832001782736 1102 68-69 0.06787824841716628 1102 70-71 -0.007010685386582338 1102 72-73 0.10704877231502152 1102 74-75 0.1510014393449346 1102 76-77 -0.08043947113667826 1102 78-79 0.058528863611702064 1102 80-81 0.07384691694218759 1102 82-83 0.17004132197461885 1102 84-85 0.11837469445280036 1102 86-87 0.25995308030432085 1102 88-89 0.16231897767405812 1102 90-91 0.034573521941293706 1102 92-93 -0.18164243690286952 1102 94-95 -0.2943012550710584 1102 96-97 -0.12862176125408453 1102 98-99 -0.2060250045223846 1102 100-101 -0.15961969166984602 1103 1 -0.0018590133074738446 1103 2 0.12995564691357941 1103 3 0.05514208529401543 1103 4 0.007994152564243961 1103 5 -0.029416965243370896 1103 6 0.06079792726586675 1103 7 0.04392963802379768 1103 8 -0.03994970537765141 1103 9 0.009084272364177082 1103 10-11 -0.008856245632429705 1103 12-13 0.005158383870906391 1103 14-15 0.04706840935043033 1103 16-17 0.13371269439993938 1103 18-19 0.0648063559901928 1103 20-21 -0.0686418319616493 1103 22-23 0.04806469375433409 1103 24-25 0.03840878858592589 1103 26-27 -0.05395369746606349 1103 28-29 -0.11299278329974527 1103 30-31 0.08779074021328626 1103 32-33 0.1994710269891513 1103 34-35 0.1548123613305492 1103 36-37 0.3174057898587108 1103 38-39 0.4293840888184306 1103 40-41 0.22288131736438999 1103 42-43 0.3566855382181515 1103 44-45 0.2582538726779404 1103 46-47 0.5154983042193777 1103 48-49 0.6026110943119534 1103 50-51 0.4386543281436488 1103 52-53 0.5584598997016457 1103 54-55 0.6873299660462635 1103 56-57 0.800911552249822 1103 58-59 0.8407325997826973 1103 60-61 0.5640044359094034 1103 62-63 0.733455494290169 1103 64-65 0.7501479244255691 1103 66-67 0.9652965164271947 1103 68-69 0.8449031512052798 1103 70-71 0.8474031476571824 1103 72-73 0.7004300910872523 1103 74-75 0.7701956149054503 1103 76-77 0.2685635900033887 1103 78-79 0.6286124244622009 1103 80-81 0.7903191598477903 1103 82-83 0.6740621708204877 1103 84-85 0.5357487326756853 1103 86-87 0.7377804981913059 1103 88-89 0.8193802832089325 1103 90-91 0.6016124894270227 1103 92-93 0.40368891082609437 1103 94-95 0.37157944319980984 1103 96-97 0.4167079318045701 1103 98-99 0.3209295747776899 1103 100-101 0.5315278221880178 1104 1 0.05353219931406272 1104 2 0.09788966513653463 1104 3 -0.002500243330707974 1104 4 -0.008470716267162004 1104 5 -0.2051511577462719 1104 6 0.0013478263669526314 1104 7 0.06383615364784134 1104 8 0.07287936268325979 1104 9 0.0721575616688952 1104 10-11 0.09882086254344813 1104 12-13 0.026359577841432724 1104 14-15 0.06343868544655606 1104 16-17 -0.155366526538117 1104 18-19 -0.06837066045939366 1104 20-21 -0.023483833429274625 1104 22-23 -0.1416300488005504 1104 24-25 -0.3357943392805254 1104 26-27 -0.31155933557528215 1104 28-29 -0.40464511773379996 1104 30-31 -0.4565828074538061 1104 32-33 -0.39739002297110204 1104 34-35 -0.6031114523427803 1104 36-37 -0.5454452542922112 1104 38-39 -0.4291701519936524 1104 40-41 -0.3635019150700245 1104 42-43 -0.33707095906063955 1104 44-45 -0.20168467070858753 1104 46-47 -0.6516661686602134 1104 48-49 -0.9128705833618724 1104 50-51 -0.9193992400089854 1104 52-53 -0.7040808038389841 1104 54-55 -0.5885741111872704 1104 56-57 -0.6875271134384349 1104 58-59 -0.7986502733904146 1104 60-61 -0.9723745924034475 1104 62-63 -0.8166261038875362 1104 64-65 -0.8599455981696735 1104 66-67 -0.956483890837518 1104 68-69 -0.8558340988405782 1104 70-71 -0.7648946723146892 1104 72-73 -0.7347404433712548 1104 74-75 -0.7793738448267042 1104 76-77 -0.7513811449076364 1104 78-79 -0.6587478840438798 1104 80-81 -0.5005305695596576 1104 82-83 -0.6708678496650506 1104 84-85 -0.5884782822451236 1104 86-87 -0.558514524128757 1104 88-89 -0.355458622496446 1104 90-91 -0.1494347190362575 1104 92-93 -0.2819230563970443 1104 94-95 -0.5361668418854961 1104 96-97 -0.6625349823396256 1104 98-99 -0.39826090250476653 1104 100-101 -0.16487153878550131 1105 1 -0.062232056408539904 1105 2 -0.039300501462413706 1105 3 0.011298348027821703 1105 4 0.003143803575717641 1105 5 -0.06022785421112076 1105 6 0.08441873250266951 1105 7 -0.0163776821912478 1105 8 -0.08094106939042689 1105 9 -0.02699759206264929 1105 10-11 -0.014636573458183477 1105 12-13 -0.002547624515337077 1105 14-15 0.12477257749586101 1105 16-17 -0.0985552709509534 1105 18-19 0.03360770199489593 1105 20-21 -0.003645183828837162 1105 22-23 -0.06455475759101859 1105 24-25 -0.016729426643379952 1105 26-27 1.7936536330864783E-5 1105 28-29 0.07730454703662559 1105 30-31 -0.2029278738224889 1105 32-33 -0.0819313679528122 1105 34-35 -0.024851006047235558 1105 36-37 -0.043153249009804995 1105 38-39 0.21373415519854433 1105 40-41 -0.0032316997104118173 1105 42-43 0.04028242032443785 1105 44-45 -0.030125006615406846 1105 46-47 -0.08211661224683553 1105 48-49 -0.12915080481656105 1105 50-51 -0.024810319515957246 1105 52-53 0.2500092510167633 1105 54-55 0.23365474225983718 1105 56-57 0.1210280132038477 1105 58-59 0.18337557992123976 1105 60-61 0.09278777384333381 1105 62-63 0.3254083841254669 1105 64-65 0.42273293697864034 1105 66-67 0.3449349091082965 1105 68-69 0.4820832450575665 1105 70-71 0.4879285364080559 1105 72-73 0.5233397746972877 1105 74-75 0.5628333372131173 1105 76-77 0.7173095973380619 1105 78-79 0.5153916087097379 1105 80-81 0.2525317865286496 1105 82-83 0.33231302193185996 1105 84-85 0.3358262856811898 1105 86-87 0.3972537949827135 1105 88-89 0.2778861391180776 1105 90-91 0.04234030466684402 1105 92-93 0.18755920013824223 1105 94-95 0.20220170748467936 1105 96-97 -0.061997853123859414 1105 98-99 -0.015496020343043426 1105 100-101 -0.0417466234394368 1106 1 0.041955631403332916 1106 2 0.03903048853014468 1106 3 0.07567405447614561 1106 4 0.04468434337115923 1106 5 -0.033828052705871414 1106 6 8.52443527399771E-4 1106 7 -0.041172984482209074 1106 8 0.10156106718498847 1106 9 -0.08023278077050833 1106 10-11 0.01567109340146544 1106 12-13 -0.05510324439789116 1106 14-15 -0.06727384822023907 1106 16-17 0.023043438833184382 1106 18-19 -0.03981881359521111 1106 20-21 -0.053526638354242095 1106 22-23 0.07654881807132341 1106 24-25 -0.018131295802263026 1106 26-27 0.05487967943049199 1106 28-29 -0.08897202231248258 1106 30-31 0.06603526680552818 1106 32-33 0.15443038872570725 1106 34-35 0.2894998885575646 1106 36-37 0.15471706775473137 1106 38-39 0.2523083012575924 1106 40-41 0.23621913148634377 1106 42-43 0.029839391074027333 1106 44-45 0.32633572175004133 1106 46-47 0.7267425943255432 1106 48-49 0.4988659018247432 1106 50-51 0.46445993659740026 1106 52-53 0.3591730918901348 1106 54-55 0.31374048096355267 1106 56-57 0.39437457952039523 1106 58-59 0.507389309141324 1106 60-61 0.4181385857419926 1106 62-63 0.6139205005757162 1106 64-65 0.5530654156818073 1106 66-67 0.937638848677679 1106 68-69 0.9635170939258728 1106 70-71 0.9634792214906227 1106 72-73 0.712549379637597 1106 74-75 0.782906118620776 1106 76-77 0.681744576523684 1106 78-79 0.504477154432454 1106 80-81 0.6149597632606572 1106 82-83 0.4844551110890869 1106 84-85 0.4855850594247464 1106 86-87 0.8307842446284575 1106 88-89 0.7792025452603859 1106 90-91 0.7713136457194167 1106 92-93 0.6631664010866025 1106 94-95 0.3749624386070032 1106 96-97 0.5262744155057 1106 98-99 0.34035130411316317 1106 100-101 0.3247518740655977 1107 1 -0.10514171372941661 1107 2 0.04193314339740084 1107 3 -0.005848069417659474 1107 4 0.06926841416761675 1107 5 -0.11703159252888184 1107 6 -0.01741304319826753 1107 7 -2.2542004330716736E-5 1107 8 0.061738058335429 1107 9 -0.1540380905050185 1107 10-11 -0.008842180934820476 1107 12-13 0.01932153436316497 1107 14-15 0.004009337620466624 1107 16-17 -0.03277957001637333 1107 18-19 -0.13892500828547583 1107 20-21 -0.10219035516839625 1107 22-23 0.1064514729385806 1107 24-25 -0.038131295802259046 1107 26-27 0.07513631659863762 1107 28-29 -0.14023750903814403 1107 30-31 -0.026566503105975414 1107 32-33 -0.3581182838406676 1107 34-35 -0.3164647132123477 1107 36-37 -0.19692894994438603 1107 38-39 -0.39245276068930934 1107 40-41 -0.3282410455048037 1107 42-43 -0.08050574166933444 1107 44-45 -0.3313368446216316 1107 46-47 -0.5428237773558635 1107 48-49 -1.1681694964053548 1107 50-51 -1.0674427182698523 1107 52-53 -1.0447384125346417 1107 54-55 -1.0542860677090076 1107 56-57 -1.0357227656123484 1107 58-59 -0.8726991864338984 1107 60-61 -0.9879941576208395 1107 62-63 -1.0199467560614508 1107 64-65 -1.1320053807783736 1107 66-67 -1.0029186734462172 1107 68-69 -0.929801490144925 1107 70-71 -0.94064467231469 1107 72-73 -0.6339904433712569 1107 74-75 -0.4471292796093138 1107 76-77 -0.42860940577720186 1107 78-79 -0.5502750579569273 1107 80-81 -0.6192968739074871 1107 82-83 -0.8306776322737477 1107 84-85 -0.8474945865929513 1107 86-87 -0.655144958911368 1107 88-89 -0.6308771007573135 1107 90-91 -0.7042173277319108 1107 92-93 -0.5467274042231303 1107 94-95 -0.9554711897115808 1107 96-97 -0.6872034606004931 1107 98-99 -0.43603807641781245 1107 100-101 -0.06246936487245591 1108 1 -0.028963495907632364 1108 2 -0.08535398531547145 1108 3 -0.04818470308102718 1108 4 0.04841692901910477 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-1.2784147351659136 2316 90-91 -1.8920130266566417 2316 92-93 -1.968254285943562 2316 94-95 -1.832041082184702 2316 96-97 -1.775343245546729 2316 98-99 -1.7768767860952295 2316 100-101 -1.8311145261627786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 11.0 6 17.0 7 35.0 8 90.0 9 187.0 10 362.0 11 655.0 12 1264.0 13 1841.0 14 2329.0 15 3707.0 16 5610.0 17 9361.0 18 8841.0 19 3951.0 20 2689.0 21 2553.0 22 2485.0 23 2489.0 24 2661.0 25 2973.0 26 3427.0 27 3989.0 28 4909.0 29 6203.0 30 7841.0 31 10065.0 32 13288.0 33 18104.0 34 27186.0 35 41366.0 36 68729.0 37 120253.0 38 127012.0 39 16771.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.30226493160847 20.15093170789533 15.317063657464944 19.229739703031253 2 15.270037839697281 22.548446279096435 40.5519244734931 21.629591407713182 3 20.32412185146963 27.96066964797615 30.650919237090545 21.06428926346367 4 11.97148645033062 18.034629056300883 39.43660895157283 30.557275541795665 5 12.157347696283242 40.05634578113831 33.46018142271186 14.32612509986659 6 28.356648702365938 38.51106524481138 18.10610404005657 15.026182012766121 7 26.163284027061117 36.21832358674464 20.73214080954019 16.886251576654054 8 26.35305584221993 34.4480755265069 19.643198409968278 19.555670221304897 9 27.866452623934563 17.079081145128615 21.05396934602301 34.00049688491381 10-11 23.991419179757674 28.58770018728739 26.681190994916488 20.73968963803845 12-13 26.899661048694508 26.213349158068656 27.644742336805027 19.24224745643181 14-15 23.667870657034744 26.365669074647403 28.4551656920078 21.511294576310057 16-17 19.92002063983488 30.401616787065706 28.856209150326794 20.82215342277262 18-19 19.741333180445668 28.11078622482131 33.183407866070404 18.964472728662614 20-21 21.35926690364255 26.732981691702022 32.97462064747926 18.933130757176166 22-23 20.721989647366403 26.837535129102136 32.43458891903178 20.00588630449968 24-25 20.424819114089036 26.970729544507684 33.20885681633152 19.395594525071765 26-27 20.842072874153477 26.714853190047865 33.36346583671687 19.07960809908179 28-29 19.993578540092557 27.05699332340173 33.91104175764531 19.0383863788604 30-31 20.105360021480983 27.3177341384243 33.39041210426686 19.186493735827852 32-33 19.794557199097962 27.575774949355964 32.96821847647441 19.661449375071665 34-35 20.304724228872836 26.940813362381988 33.221916446890646 19.532545961854527 36-37 20.80845318327366 26.925506226845812 33.04561703249923 19.220423557381295 38-39 20.70605772492511 26.935495430070283 33.2989015924282 19.05954525257641 40-41 20.22743973114678 27.283976688430883 33.37359379046152 19.114989789960813 42-43 19.950937810282035 28.022594982736493 32.54761201689176 19.478855190089707 44-45 20.478442839123954 27.69884952031495 31.771872491686732 20.050835148874366 46-47 20.576577609601348 28.239116309291745 30.87566410579827 20.308641975308642 48-49 20.87241524290028 28.32874670335971 30.657225853304286 20.14161220043573 50-51 21.233230134158926 28.82400718572029 30.30357757137943 19.63918510874135 52-53 20.850286378256275 29.21325767927546 29.972824165470634 19.963631776997627 54-55 20.13989221419562 29.00957459006995 30.420632190498033 20.429901005236403 56-57 21.07852692734014 29.41262469900241 29.417211328976034 20.09163704468142 58-59 21.033711730306155 28.75988992088063 29.669762641898867 20.536635706914343 60-61 20.666590222833772 28.91268203187708 30.16234759010817 20.25838015518098 62-63 21.32123609677789 28.4324236517219 30.28140885984023 19.96493139165998 64-65 20.951439055154225 28.741925620150592 30.220349348316322 20.086285976378857 66-67 20.647288155028093 28.741447846195005 30.0476818407675 20.563582158009403 68-69 20.76071169208424 29.061078622482135 29.817299239383864 20.360910446049765 70-71 20.802946909758056 29.323376524098922 29.408993616939956 20.464682949203073 72-73 20.806673546611627 28.646657493406718 29.43594006803501 21.110728891946643 74-75 20.579348698543747 29.304265565875472 29.201830829797803 20.914554905782975 76-77 20.884264036998815 28.707812559721745 29.632209608989797 20.775713794289647 78-79 20.72898750143332 28.44283912395367 29.645300615372854 21.182872759240148 80-81 20.69286779039101 29.431735657225854 29.24607269808508 20.629323854298054 82-83 20.510990265805525 29.485426419255496 29.111806114950834 20.89177719998815 84-85 20.45944698567346 28.770046219896628 28.918825171831568 21.85168162259835 86-87 20.483220578679813 29.04244543821427 28.936570729656385 21.537763253449526 88-89 20.819764552994688 28.16697244199824 29.2406260749914 21.772636930015672 90-91 20.425894638666126 28.086130711659163 29.555375476847566 21.932599172827143 92-93 20.363706206569685 28.500798850267174 29.38077637543669 21.75471856772645 94-95 20.006975499751558 28.613404426097926 29.158066735466114 22.2215533386844 96-97 19.60201429499675 28.630890952872377 29.247314910369603 22.519779841761267 98-99 19.882924703728406 28.746610148436798 28.780914777363915 22.58955037047088 100-101 19.95682417846006 29.673104177198617 28.12573166198244 22.24433998235888 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 391.0 1 265.0 2 172.0 3 236.5 4 228.5 5 229.5 6 386.5 7 728.0 8 1175.5 9 1421.0 10 1402.0 11 1376.5 12 1418.0 13 1454.5 14 1541.5 15 1661.0 16 1722.0 17 1757.5 18 1805.0 19 1924.5 20 2045.0 21 2184.0 22 2418.0 23 2697.5 24 3000.5 25 3453.0 26 4163.5 27 4991.5 28 5804.5 29 6886.0 30 8231.5 31 9168.0 32 10184.5 33 11659.5 34 13331.0 35 14616.5 36 16199.5 37 17462.5 38 17777.5 39 17936.5 40 17863.5 41 17853.5 42 17814.0 43 18129.5 44 18837.0 45 18993.5 46 18814.0 47 20136.5 48 27223.5 49 26235.0 50 18484.5 51 15915.5 52 13247.0 53 11852.5 54 10848.5 55 9568.5 56 8278.0 57 7173.5 58 6499.0 59 5781.0 60 4836.0 61 3879.5 62 2965.0 63 2042.0 64 1290.5 65 928.0 66 668.5 67 467.0 68 330.5 69 226.5 70 171.0 71 130.0 72 100.5 73 93.0 74 71.5 75 70.0 76 57.5 77 29.5 78 19.5 79 12.0 80 6.0 81 3.5 82 2.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04624851890073768 2 0.0 3 0.0 4 0.0 5 0.0118487940985361 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005446623093681917 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0021977601956962123 24-25 3.822191644689065E-4 26-27 0.002962198524634025 28-29 0.002771088942399572 30-31 0.0012422122845239462 32-33 0.0 34-35 0.0 36-37 2.866643733516798E-4 38-39 4.7777395558613303E-4 40-41 9.555479111722662E-5 42-43 0.008886595573902076 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.9110958223445324E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.866643733516798E-4 84-85 9.555479111722662E-5 86-87 0.0 88-89 0.0 90-91 0.006115506631502504 92-93 0.001528876657875626 94-95 0.0 96-97 0.0 98-99 0.0013377670756411727 100-101 0.009651033902839888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 523260.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.70225159379841 #Duplication Level Percentage of deduplicated Percentage of total 1 83.42242751002313 52.30774038298879 2 10.248328253557283 12.851865131407628 3 2.76497677058181 5.2011080736008655 4 1.144149198830553 2.8696292370366483 5 0.6233541316250149 1.9542853796592707 6 0.38344376460826285 1.4425672440324306 7 0.23734880947177042 1.041761333689123 8 0.168455810323585 0.8450046881077289 9 0.1361525001996471 0.7683361490378663 >10 0.777967226793201 8.890994658401512 >50 0.05253526433103213 2.2376623139956244 >100 0.035023508275505176 3.981983157813655 >500 0.0033794613248294223 1.4403409641997278 >1k 0.002150566297618723 3.0962418506864013 >5k 3.0722375680267475E-4 1.0704794353427696 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5557 1.0619959484768566 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4318 0.825211176088369 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2932 0.5603332951114169 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2662 0.5087337079081146 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1877 0.35871268585406874 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1760 0.3363528647326377 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1337 0.255513511447464 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1187 0.226847074112296 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 759 0.14505217291595002 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 753 0.1439055154225433 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 750 0.1433321866758399 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 706 0.13492336505752398 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 703 0.13435003631082063 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 692 0.13224783090624165 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 685 0.13091006383060047 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 666 0.12727898176814587 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 652 0.12460344761686351 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 558 0.1066391468868249 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 553 0.10568359897565265 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.555479111722662E-5 0.0 18-19 0.0 0.0 0.0 3.822191644689065E-4 0.0 20-21 0.0 0.0 0.0 3.822191644689065E-4 0.0 22-23 0.0 0.0 0.0 5.733287467033597E-4 0.0 24-25 0.0 0.0 0.0 0.0014333218667583994 0.0 26-27 0.0 0.0 0.0 0.0018155410312273058 0.0 28-29 0.0 0.0 0.0 0.0037266368535718384 0.0 30-31 0.0 0.0 0.0 0.007453273707143676 0.0 32-33 0.0 0.0 0.0 0.01098880097848106 0.0 34-35 0.0 0.0 0.0 0.016817643236631884 0.0 36-37 0.0 0.0 0.0 0.024175362152658336 0.0 38-39 0.0 0.0 0.0 0.03143752627756756 0.0 40-41 0.0 0.0 0.0 0.0398463478958835 0.0 42-43 0.0 0.0 0.0 0.05437067614570194 0.0 44-45 0.0 0.0 0.0 0.07739938080495357 0.0 46-47 0.0 0.0 0.0 0.13540113901311013 0.0 48-49 0.0 0.0 0.0 0.255513511447464 0.0 50-51 0.0 0.0 0.0 0.33014180331001797 0.0 52-53 0.0 0.0 0.0 0.4082100676527921 0.0 54-55 0.0 0.0 0.0 0.5191491801398922 0.0 56-57 0.0 0.0 0.0 0.6004663073806521 0.0 58-59 0.0 0.0 0.0 0.7186675839926614 0.0 60-61 0.0 0.0 0.0 0.9458968772694263 0.0 62-63 0.0 0.0 0.0 1.0802469135802468 0.0 64-65 0.0 0.0 0.0 1.2561632840270611 0.0 66-67 0.0 0.0 0.0 1.5686274509803921 0.0 68-69 0.0 0.0 0.0 1.8650384130260291 0.0 70-71 0.0 0.0 0.0 2.1843825249398003 0.0 72-73 0.0 0.0 0.0 2.5726216412490923 0.0 74-75 0.0 0.0 0.0 2.905725643083744 0.0 76-77 0.0 0.0 0.0 3.15187478500172 0.0 78-79 0.0 0.0 0.0 3.496349806979322 0.0 80-81 0.0 0.0 0.0 3.8740778962657187 0.0 82-83 0.0 0.0 0.0 4.286587929518786 0.0 84-85 0.0 0.0 0.0 4.709991208959217 0.0 86-87 0.0 0.0 0.0 5.525169131980277 0.0 88-89 0.0 0.0 0.0 6.750754882849826 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAACGC 25 0.0050204303 56.99805 3 CGGGTCG 60 0.003949664 31.665585 6 TAAGGTG 200 0.0 30.873945 5 GGTAAGG 140 1.702756E-8 30.534668 3 CCTATAC 135 4.218964E-7 28.147184 3 TACCTGG 530 0.0 26.885872 2 GAGTACT 4145 0.0 25.155342 12-13 CTACACT 170 1.1207521E-7 25.1462 4 CCCTATA 95 0.0012259064 24.999146 2 GTAAGGT 190 1.1883458E-8 24.999146 4 GTACTTT 4310 0.0 23.914495 14-15 GTCTAGG 145 2.319784E-5 22.945597 1 AGTACTT 4225 0.0 22.711454 12-13 GTACATG 4715 0.0 21.673353 1 ACCTGGG 685 0.0 21.495617 3 TACATGG 4770 0.0 21.209967 2 ACATGGG 4695 0.0 20.941776 3 GGTGTGC 275 1.2532837E-9 20.728544 8 TCCCGCT 115 0.0037192847 20.65344 8 AAGGGTA 140 4.8479988E-4 20.356445 4 >>END_MODULE