FastQCFastQC Report
Thu 26 May 2016
SRR937301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603945
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49230.8151404515311825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.6182682197882258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.4059972348475441No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23170.3836442060121369No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21210.3511909197029531No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16870.2793300714469033No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11480.19008353409664788No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG11230.1859440843123132No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11170.1849506163640729No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT10570.1750159368816697No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9720.16094180761493182No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.1549809999254899No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC8970.14852345826192784No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8260.13676742087441737No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7380.12219655763355934No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG753.0754437E-431.668919
CCCTTAC856.369941E-427.9663731
TACCTGG6600.025.923842
GTACATG50650.024.5927411
TACATGG51050.024.3919372
GTAGCCC2950.024.1662923
GAGTACT36450.023.65295612-13
GTGTAGC3500.023.0922341
ACATGGG51300.022.9760343
ATGGGAT13900.022.226725
GTCGGGA1503.0168329E-522.1792852
CATGGGG11000.022.0370944
GTACCTG7900.021.665091
AGTACTT37550.021.5685412-13
GTACTTT39800.021.48036614-15
CGGGAGT1553.8828675E-521.4656094
CATGGGA37700.021.054844
TAGGACA3401.8189894E-1220.9695534
ATCGTAT1200.004736148819.8095171
CTAGGAC4800.019.8029353
Produced by FastQC (version 0.11.5)