##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937301_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 603945 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00688142132148 33.0 31.0 34.0 31.0 34.0 2 32.099220955550585 33.0 31.0 34.0 30.0 34.0 3 32.13401220309797 34.0 31.0 34.0 30.0 34.0 4 35.73971470912086 37.0 35.0 37.0 35.0 37.0 5 35.73507521380258 37.0 35.0 37.0 35.0 37.0 6 35.82137446290639 37.0 35.0 37.0 35.0 37.0 7 35.79080876569886 37.0 35.0 37.0 35.0 37.0 8 35.79580756525843 37.0 35.0 37.0 35.0 37.0 9 37.455132503787596 39.0 37.0 39.0 35.0 39.0 10-11 37.36844745796388 39.0 37.0 39.0 35.0 39.0 12-13 37.280451034448504 39.0 37.0 39.0 34.5 39.0 14-15 38.443979170288685 40.0 38.0 41.0 34.0 41.0 16-17 38.41041402776743 40.0 38.0 41.0 34.0 41.0 18-19 38.436741756285755 40.0 38.0 41.0 34.0 41.0 20-21 38.41416022982226 40.0 38.0 41.0 34.0 41.0 22-23 38.29108859250428 40.0 38.0 41.0 34.0 41.0 24-25 38.15503481277268 40.0 38.0 41.0 34.0 41.0 26-27 37.976872066164965 40.0 38.0 41.0 33.5 41.0 28-29 37.801380920448054 40.0 37.5 41.0 33.0 41.0 30-31 37.74906489829372 40.0 37.0 41.0 33.0 41.0 32-33 37.56572866734554 40.0 37.0 41.0 33.0 41.0 34-35 37.363498331801736 40.0 36.0 41.0 33.0 41.0 36-37 37.353316113222235 40.0 37.0 41.0 33.0 41.0 38-39 37.30593348732087 40.0 37.0 41.0 33.0 41.0 40-41 37.12680128157365 40.0 36.5 41.0 32.5 41.0 42-43 36.863192840407656 40.0 36.0 41.0 31.0 41.0 44-45 36.56258765284918 40.0 36.0 41.0 30.5 41.0 46-47 36.107190224275385 40.0 36.0 41.0 29.5 41.0 48-49 35.70045782314615 39.0 36.0 41.0 27.5 41.0 50-51 34.76166952288702 38.5 34.0 40.0 25.5 40.5 52-53 34.85428722814163 38.5 35.0 40.0 26.0 40.5 54-55 35.21476707316064 39.0 35.0 40.5 26.0 41.0 56-57 35.03973789003965 39.0 35.0 41.0 25.5 41.0 58-59 34.83619452102427 39.0 35.0 40.5 24.5 41.0 60-61 34.56311005141197 38.5 34.5 40.0 23.5 41.0 62-63 34.24893243590062 38.0 34.0 40.0 22.0 41.0 64-65 33.90727880850078 38.0 34.0 40.0 22.0 41.0 66-67 33.522538476185744 37.0 34.0 40.0 19.5 41.0 68-69 33.06743991588638 36.5 33.0 39.0 18.0 41.0 70-71 32.63529046519136 36.0 33.0 39.0 13.5 40.5 72-73 32.21464040599723 35.5 32.0 39.0 9.5 40.0 74-75 31.762085123645367 35.0 32.0 38.0 7.0 40.0 76-77 31.301680616612437 35.0 31.5 37.0 4.0 39.0 78-79 30.83482684681552 35.0 31.0 37.0 2.0 39.0 80-81 30.37605079932775 35.0 31.0 36.0 2.0 38.5 82-83 29.938611131808358 34.5 30.5 36.0 2.0 37.0 84-85 29.47569729031617 34.0 30.0 35.0 2.0 37.0 86-87 29.05665913286806 34.0 29.0 35.0 2.0 36.5 88-89 28.677637864375065 34.0 29.0 35.0 2.0 36.0 90-91 28.239402594607125 34.0 29.0 35.0 2.0 36.0 92-93 27.84912533426057 34.0 28.0 35.0 2.0 35.0 94-95 27.3893127685468 34.0 27.0 35.0 2.0 35.0 96-97 26.82403861278759 33.5 25.5 35.0 2.0 35.0 98-99 26.18681668032685 33.0 25.0 35.0 2.0 35.0 100-101 24.45567642748926 31.5 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.13590436925845495 1101 2 -0.1360779322019816 1101 3 0.011233013753091825 1101 4 -0.011005285463213 1101 5 -0.10730949770609755 1101 6 -0.0726539329485405 1101 7 -0.09411294871019749 1101 8 -0.20579881054277394 1101 9 -0.22625043308562454 1101 10-11 -0.23779886645306902 1101 12-13 -0.14594894876523767 1101 14-15 -0.18124426981425046 1101 16-17 -0.06224905640802092 1101 18-19 -0.060633826391985224 1101 20-21 -0.024260642955056255 1101 22-23 -0.1835996416846868 1101 24-25 -0.13184113343564263 1101 26-27 -0.10501828079198816 1101 28-29 -0.2642772429705005 1101 30-31 -0.26703928278526945 1101 32-33 -0.24887291771234032 1101 34-35 -0.21774311192547913 1101 36-37 -0.2677174455245108 1101 38-39 -0.16851262077778983 1101 40-41 -0.24397188468866915 1101 42-43 -0.011051107599804766 1101 44-45 0.03838527596297325 1101 46-47 0.18559794292428222 1101 48-49 0.17953628536691468 1101 50-51 0.33181235574299706 1101 52-53 0.30578665056235366 1101 54-55 0.24492494629620865 1101 56-57 0.3072906012601564 1101 58-59 0.3334854440342312 1101 60-61 0.3530979978066995 1101 62-63 0.6072671554192652 1101 64-65 0.5742858366975128 1101 66-67 0.6373948927570154 1101 68-69 0.34728703056704546 1101 70-71 0.26641919900549027 1101 72-73 0.3198341369742401 1101 74-75 0.45095167918734447 1101 76-77 0.4576817774240354 1101 78-79 0.656438742884685 1101 80-81 0.5844617147178646 1101 82-83 0.5027888888978964 1101 84-85 0.6643918566135198 1101 86-87 0.6861568342231372 1101 88-89 0.59580915298368 1101 90-91 0.6850786825126249 1101 92-93 0.5369192636357525 1101 94-95 0.4645036727329632 1101 96-97 0.6648130276220918 1101 98-99 0.4763197973988085 1101 100-101 0.346056796502598 1102 1 0.04318523781546091 1102 2 -0.18633012723280373 1102 3 0.06918597886092925 1102 4 -0.14962952524567896 1102 5 -0.014817229611360005 1102 6 -0.022598927447987194 1102 7 -0.18332564356458647 1102 8 -0.06399878148326366 1102 9 -0.009196652235843317 1102 10-11 0.04582194845860954 1102 12-13 0.029103462136227165 1102 14-15 -0.024421512066780338 1102 16-17 0.10936291610998694 1102 18-19 0.05648118699614457 1102 20-21 0.01929748644278817 1102 22-23 0.18521223950343568 1102 24-25 0.15445211853106144 1102 26-27 0.029848979518945384 1102 28-29 -0.05295233689498957 1102 30-31 0.04823045173534979 1102 32-33 0.030513511496614854 1102 34-35 9.71212337134375E-4 1102 36-37 -0.03361714870236909 1102 38-39 0.03524124517484495 1102 40-41 0.004009517225107118 1102 42-43 0.1501305766063581 1102 44-45 0.4033415829144289 1102 46-47 0.5971584385964945 1102 48-49 0.6397455138369352 1102 50-51 0.6485506333443425 1102 52-53 0.33343469838601436 1102 54-55 0.7102507241570137 1102 56-57 0.6554494737511831 1102 58-59 0.6108844103459603 1102 60-61 0.6425774174467804 1102 62-63 0.7615939088493278 1102 64-65 0.7417931534669293 1102 66-67 0.7685103627945793 1102 68-69 0.7764440972171016 1102 70-71 0.7377644467378133 1102 72-73 0.4627135192520875 1102 74-75 0.6601957167986576 1102 76-77 0.49450432760358254 1102 78-79 0.9878883972840882 1102 80-81 0.9905527955240565 1102 82-83 0.8820154908033722 1102 84-85 1.1007515303167388 1102 86-87 1.046972161038834 1102 88-89 0.9715278569295478 1102 90-91 0.8330746141812639 1102 92-93 0.6706552787466968 1102 94-95 0.9038004325976274 1102 96-97 1.0391669724505341 1102 98-99 0.9212935400560909 1102 100-101 1.129075664427127 1103 1 -0.15613660140064312 1103 2 -0.15086043008430394 1103 3 -0.1396579091035548 1103 4 -0.12399919105612156 1103 5 -0.27156113999763676 1103 6 -0.07735529700627808 1103 7 -0.010173048209701108 1103 8 -0.07924428696348684 1103 9 -0.022178999338251515 1103 10-11 -0.06521770139433869 1103 12-13 -0.004931764986011444 1103 14-15 -0.01150603696742536 1103 16-17 -0.16271369560362814 1103 18-19 -0.18703780137467874 1103 20-21 -0.06588826693588601 1103 22-23 -0.07086622889167415 1103 24-25 -0.02297700697982208 1103 26-27 -0.15796890834850075 1103 28-29 -0.3134535360017878 1103 30-31 -0.14813525158110252 1103 32-33 -0.15496057822463172 1103 34-35 -0.05218088475577076 1103 36-37 0.09453058859817531 1103 38-39 -4.0481049150997706E-4 1103 40-41 0.07148926877594874 1103 42-43 0.1781722365530456 1103 44-45 0.1244191137902746 1103 46-47 0.36537483115350966 1103 48-49 0.5808863502090063 1103 50-51 0.48815551159140824 1103 52-53 0.4854367281479881 1103 54-55 0.28287568629566096 1103 56-57 0.5329504538849505 1103 58-59 0.42977470223569725 1103 60-61 0.45860008049973544 1103 62-63 0.47642480791062525 1103 64-65 0.41060890274149386 1103 66-67 0.5981591798812929 1103 68-69 0.6941135161256256 1103 70-71 0.6570846862707 1103 72-73 0.8575350037843315 1103 74-75 0.8900544595263291 1103 76-77 0.7519936641415264 1103 78-79 0.9223794029996775 1103 80-81 0.8549088378028991 1103 82-83 0.9951065376911323 1103 84-85 0.8320480211100367 1103 86-87 0.869584994431829 1103 88-89 0.8513582118363132 1103 90-91 0.9324831247581855 1103 92-93 1.0755921371524408 1103 94-95 0.9495887420602784 1103 96-97 0.8116966092044358 1103 98-99 1.0344003215328073 1103 100-101 1.4768441719095797 1104 1 -0.12252851665857989 1104 2 -0.013343814517028818 1104 3 0.0037357718683992402 1104 4 -0.07740540142484775 1104 5 -0.029089079331548362 1104 6 -0.07272506682249258 1104 7 -0.009643486616496943 1104 8 0.0036206565168797056 1104 9 -0.13416830433062898 1104 10-11 0.045167657677417594 1104 12-13 0.0022859916567838923 1104 14-15 0.10777198897346096 1104 16-17 0.022206313125742838 1104 18-19 -0.04900612229815948 1104 20-21 0.14735184117402866 1104 22-23 0.07877765238274748 1104 24-25 0.11144007084741503 1104 26-27 0.151101245590624 1104 28-29 0.056289573211515176 1104 30-31 0.12265690846552957 1104 32-33 0.008573993601167729 1104 34-35 0.16879433267733646 1104 36-37 0.16554220385260265 1104 38-39 0.27683441857669067 1104 40-41 0.2891711319781294 1104 42-43 0.32151250353144434 1104 44-45 0.2982923455963302 1104 46-47 0.4618611060463351 1104 48-49 0.370164330366471 1104 50-51 0.148566127851538 1104 52-53 0.2040182356129705 1104 54-55 0.31770161009067266 1104 56-57 0.1940005119535968 1104 58-59 0.379080244013835 1104 60-61 0.42103554494966744 1104 62-63 0.6982889868078246 1104 64-65 0.4280829092030629 1104 66-67 0.2850211688047608 1104 68-69 0.17542624895340708 1104 70-71 0.13508727761244899 1104 72-73 0.2523361434863425 1104 74-75 0.08128571312223087 1104 76-77 0.12489201425957219 1104 78-79 -0.0029501231876771783 1104 80-81 0.13717661283536486 1104 82-83 0.28593123449041613 1104 84-85 0.5010147960799358 1104 86-87 0.40226825402841726 1104 88-89 0.4889915751323528 1104 90-91 0.49226025105480886 1104 92-93 0.28042259350634424 1104 94-95 0.17316751015045284 1104 96-97 -0.09010863116616363 1104 98-99 -0.09352695607802985 1104 100-101 -0.4775540126250881 1105 1 0.19295934723363217 1105 2 0.1337481735448307 1105 3 0.2640108863064654 1105 4 0.35275669891018424 1105 5 0.224105577678408 1105 6 0.3895723077587121 1105 7 0.2720582311700852 1105 8 0.19522135391183326 1105 9 0.3071655445625012 1105 10-11 0.22761562511379907 1105 12-13 0.2856837313747249 1105 14-15 0.43922213982101255 1105 16-17 0.3053864063432741 1105 18-19 0.4580511812469297 1105 20-21 0.16110524768907908 1105 22-23 0.1797216361935554 1105 24-25 0.2554792194328357 1105 26-27 0.28740005108179645 1105 28-29 0.2697926862510869 1105 30-31 0.2917775008344776 1105 32-33 0.4421605532351478 1105 34-35 0.607024403551371 1105 36-37 0.5275473764300642 1105 38-39 0.47234001834329575 1105 40-41 0.6352707446136705 1105 42-43 0.43265693050756937 1105 44-45 0.3919058100423811 1105 46-47 0.6483065913864579 1105 48-49 0.9168240354323132 1105 50-51 0.8231707174142286 1105 52-53 0.7926848729513836 1105 54-55 0.852898985366501 1105 56-57 0.9466533681008329 1105 58-59 1.0223912029782056 1105 60-61 1.1287802872816783 1105 62-63 1.1018820063751704 1105 64-65 0.7993380580478018 1105 66-67 0.9733108609275831 1105 68-69 0.9803235267513841 1105 70-71 0.9714055323653596 1105 72-73 0.9874580371035115 1105 74-75 1.0104186233569372 1105 76-77 0.6640973266864911 1105 78-79 0.9646518059938529 1105 80-81 0.6201399857078123 1105 82-83 0.6809688942811505 1105 84-85 0.5153875689658136 1105 86-87 0.7599737505287081 1105 88-89 0.6455720244566088 1105 90-91 0.6061495708631881 1105 92-93 0.04513630731971929 1105 94-95 0.20318651948841548 1105 96-97 0.14521817431574746 1105 98-99 0.403964320696538 1105 100-101 0.5667891464444068 1106 1 0.14916413927703331 1106 2 0.2425580740873272 1106 3 -0.03481370682192875 1106 4 0.03245832639662183 1106 5 -0.08398664655305765 1106 6 -0.031483298027907836 1106 7 0.05828110639612305 1106 8 0.1718544913440212 1106 9 -0.026808234822674137 1106 10-11 0.040327537410355774 1106 12-13 0.1285578272155874 1106 14-15 0.18908086495663667 1106 16-17 0.007225805077453629 1106 18-19 -0.09731443810207452 1106 20-21 -0.1342439837756615 1106 22-23 0.05226345355341522 1106 24-25 0.06588835144007277 1106 26-27 0.0896858783874066 1106 28-29 0.18005489239936878 1106 30-31 0.12703688148547343 1106 32-33 0.1169458154412979 1106 34-35 0.1639299189220722 1106 36-37 -0.00996110006179407 1106 38-39 -0.2368456733384363 1106 40-41 -0.11036386207710791 1106 42-43 0.024075895245367462 1106 44-45 0.17098017713099267 1106 46-47 0.5549380335202656 1106 48-49 0.9916432036059106 1106 50-51 0.5379650212117042 1106 52-53 0.46626929881033163 1106 54-55 0.45586010652382214 1106 56-57 0.6283752261478455 1106 58-59 0.609515411838963 1106 60-61 0.54566828456921 1106 62-63 0.7672724901003107 1106 64-65 0.701674744304583 1106 66-67 0.7073891837123938 1106 68-69 0.6947081334421554 1106 70-71 0.5724029329078562 1106 72-73 0.6816120832156294 1106 74-75 0.8632102146770144 1106 76-77 0.8038464225273607 1106 78-79 0.7255158430643789 1106 80-81 0.9491550173533838 1106 82-83 0.868949228820032 1106 84-85 0.6445693049513466 1106 86-87 0.33781620079995633 1106 88-89 0.4386326031184531 1106 90-91 0.5822176088378725 1106 92-93 0.5477781472835517 1106 94-95 0.5208430520381455 1106 96-97 0.5249367547859087 1106 98-99 0.5379494020708648 1106 100-101 1.0313574104588739 1107 1 0.17395809532099094 1107 2 0.048979777384033696 1107 3 0.008592886584665393 1107 4 -0.02935486041656077 1107 5 0.007496869930456285 1107 6 0.1194095591149491 1107 7 -0.04219966283464771 1107 8 0.042905315519846 1107 9 0.024428028913590083 1107 10-11 0.10253564180596442 1107 12-13 0.23703997007273614 1107 14-15 0.043854216604991336 1107 16-17 0.07973610727525227 1107 18-19 0.2884170179418746 1107 20-21 0.2326172799605999 1107 22-23 0.2319681238830853 1107 24-25 0.09370428550599996 1107 26-27 0.29911238937641116 1107 28-29 0.22483511217959062 1107 30-31 0.06390157928766627 1107 32-33 0.07003647478195774 1107 34-35 0.06496013870229689 1107 36-37 0.2458253010371081 1107 38-39 -0.05674608542634729 1107 40-41 -0.03170658185732833 1107 42-43 -0.34860611024914334 1107 44-45 -0.03104592177010801 1107 46-47 -0.01485935658961779 1107 48-49 0.07158825855096751 1107 50-51 -0.31965860516192635 1107 52-53 -0.31644017921163936 1107 54-55 -0.4442772561135442 1107 56-57 -0.31477381231368895 1107 58-59 -0.42662813211708084 1107 60-61 -0.2616634462000249 1107 62-63 -0.15067050440519125 1107 64-65 -0.23048528316794403 1107 66-67 -0.47157716244146286 1107 68-69 -0.39006178414026493 1107 70-71 -0.08039583082840096 1107 72-73 0.014012495303539652 1107 74-75 -0.05966066444386442 1107 76-77 -0.4856453357143984 1107 78-79 -0.19232412946309552 1107 80-81 -0.08036077934991326 1107 82-83 -0.07565928766348762 1107 84-85 -0.05886476098271842 1107 86-87 0.10330383816259214 1107 88-89 -0.15463662765077757 1107 90-91 0.3866475538928178 1107 92-93 0.2397252626681663 1107 94-95 0.0995003322579251 1107 96-97 0.2650123042364605 1107 98-99 0.9227708306422926 1107 100-101 1.1333491687006294 1108 1 0.005540685958699498 1108 2 -0.06376574481031128 1108 3 0.044432637385042995 1108 4 0.10031546925376489 1108 5 0.020147211714657942 1108 6 -0.029521824462101165 1108 7 3.719387920710915E-4 1108 8 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-1.8458408316006327 2316 88-89 -1.4721737297239628 2316 90-91 -1.6028354716726838 2316 92-93 -1.4962779094174792 2316 94-95 -1.7189128696604357 2316 96-97 -2.3041240576834063 2316 98-99 -1.7034441914490195 2316 100-101 -1.9871900858592433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2061.0 3 321.0 4 252.0 5 252.0 6 339.0 7 396.0 8 437.0 9 572.0 10 838.0 11 1234.0 12 1856.0 13 2630.0 14 3184.0 15 4316.0 16 5942.0 17 8815.0 18 7290.0 19 3876.0 20 3499.0 21 3385.0 22 3675.0 23 3919.0 24 4359.0 25 4790.0 26 5502.0 27 6662.0 28 7813.0 29 9329.0 30 11304.0 31 14382.0 32 18904.0 33 25807.0 34 35835.0 35 53296.0 36 80826.0 37 125250.0 38 121128.0 39 19666.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.74639034880149 19.51793824362136 14.960852201492846 20.77481920608431 2 14.401543247792869 21.255017713483166 41.88533217346265 22.45810686526132 3 19.344631342678984 27.568282786694738 30.56356126358849 22.523524607037793 4 11.391312287808482 16.941951871435805 39.987714681785356 31.679021158970354 5 12.031539195750508 39.048678258704406 34.69776320703822 14.22201933850687 6 28.166691277361423 37.57571936507726 19.293567981098985 14.96402137646233 7 26.176064564086225 34.88255946692153 21.57915870234682 17.362217266645427 8 25.480778005080296 33.86542031885399 21.053190447985052 19.600611228080655 9 26.911916221610564 16.816899024941392 21.599447848383946 34.6717369050641 10-11 23.431592414395624 27.58845847821632 28.042625673804412 20.93732343358365 12-13 25.950174192033547 25.377111855429323 28.50891785221513 20.163796100322003 14-15 22.930091649356594 25.843990363876486 28.770830153368333 22.45508783339859 16-17 19.81014910811396 29.72795837284412 29.287926415772127 21.17396610326979 18-19 19.63819702401789 27.957359408984356 32.86902393825725 19.53541962874051 20-21 21.210821590421734 26.5866929245553 32.2402132264436 19.962272258579368 22-23 20.35256250725864 26.90134886267483 31.934282348646992 20.81180628141954 24-25 20.151798564134893 26.86353474984343 32.690608811782226 20.294057874239453 26-27 20.41384333583313 26.61742844059225 32.785756475686725 20.182971747887894 28-29 19.62877857782792 26.90720708763314 33.27794737366028 20.186066960878655 30-31 19.852334851463795 27.18689596243664 32.913897945131616 20.046871240967953 32-33 19.427972218258564 27.3990456248403 32.489323010861156 20.68365914603997 34-35 20.152318666377987 26.837268218389234 32.44307958778896 20.56733352744382 36-37 20.361412901465044 26.77622645046707 32.60007747880324 20.262283169264638 38-39 20.437638939579948 26.865274229403763 32.60725306081821 20.08983377019808 40-41 19.973422742633602 27.117097537870933 32.823493499189574 20.085986220305887 42-43 19.621591356821433 27.611056964519005 32.14142629035937 20.62592538830019 44-45 19.8584396169972 27.81515519046442 31.43073546027864 20.89566973225974 46-47 20.108216436190375 27.778510501871768 30.72422725949299 21.389045802444866 48-49 20.32777420146931 27.83069423889888 30.587060252317777 21.25447130731403 50-51 20.6775147560839 28.155562099988803 30.512636517107932 20.654286626819367 52-53 20.28104703667847 28.493945268914345 30.337616484199298 20.887391210207888 54-55 19.694578845017887 28.166709807591477 30.83215525423279 21.306556093157848 56-57 20.455482352277617 28.784620067798972 29.671132684097767 21.088764895825644 58-59 20.4142109986282 28.131132258562147 30.058040298113813 21.39661644469584 60-61 20.435909218342793 27.867118772281103 30.71138619363679 20.985585815739313 62-63 20.839519648843996 27.858741326025328 30.431529721686307 20.870209303444376 64-65 20.452935604646804 28.02165809864587 30.733565242202104 20.79184105450522 66-67 20.162766719008836 28.057226329685967 30.445140680979055 21.33486627032614 68-69 20.013669247345362 28.46363631839393 30.3881137268108 21.134580707449906 70-71 20.02077055094218 28.423553340251324 29.919464345408294 21.636211763398208 72-73 20.155444540858735 27.933146735731007 29.840324659918405 22.071084063491853 74-75 20.01141381120926 28.39733003084881 29.967143818168694 21.624112339773234 76-77 20.24978660466243 27.81862267307834 30.16402200232433 21.7675687199349 78-79 20.151201198461113 27.71109522158424 30.405973724768693 21.73172985518595 80-81 19.976328161028224 28.507065056915266 29.92223294947464 21.594373832581873 82-83 19.94196444866226 28.35272275995713 29.893535124947324 21.811777666433287 84-85 19.960005009741625 27.96422845417123 29.635524708228168 22.440241827858976 86-87 20.10011404872991 27.948159668221876 29.89578019699326 22.055946086054952 88-89 20.52748317399015 26.960933132267286 30.113954016924716 22.39762967681785 90-91 20.008692651723265 27.15997465196215 30.533851574630727 22.297481121683855 92-93 19.8604404619799 27.416431426777283 30.53263066908778 22.190497442155042 94-95 19.322522606220826 27.432226960567586 30.50605915567497 22.739191277536623 96-97 19.061110977482024 27.47999226978773 30.40984225224545 23.049054500484797 98-99 19.263150385868517 27.65471237367202 30.001443274341565 23.08069396611789 100-101 19.65765462397662 27.774879466659147 30.0762306510086 22.491235258355637 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 876.0 1 528.0 2 214.5 3 254.5 4 235.5 5 234.5 6 364.0 7 732.0 8 1214.5 9 1511.5 10 1432.5 11 1229.5 12 1240.0 13 1329.0 14 1421.0 15 1497.5 16 1573.0 17 1648.0 18 1698.0 19 1808.5 20 1957.0 21 2196.0 22 2501.5 23 2850.5 24 3289.0 25 3855.0 26 4710.0 27 5843.5 28 6727.5 29 7812.0 30 9396.5 31 10684.5 32 11978.0 33 13618.0 34 15647.0 35 17168.0 36 18777.0 37 20490.5 38 20884.0 39 20649.5 40 20575.0 41 20814.0 42 20795.0 43 21125.5 44 22165.0 45 22530.5 46 22263.0 47 23700.0 48 31290.5 49 30555.0 50 21910.0 51 18346.5 52 15547.5 53 13932.5 54 12599.0 55 11024.5 56 9632.0 57 8265.5 58 7282.0 59 6460.0 60 5541.0 61 4588.5 62 3459.0 63 2384.5 64 1545.5 65 1083.5 66 773.5 67 555.0 68 398.5 69 289.0 70 213.5 71 162.0 72 120.5 73 101.5 74 84.0 75 64.0 76 45.0 77 26.0 78 14.5 79 9.5 80 7.5 81 4.0 82 4.0 83 4.5 84 4.0 85 4.0 86 2.5 87 1.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28810570498969273 2 0.2614476483785775 3 0.26442805222329846 4 0.26492478619741866 5 0.2516785468875477 6 0.20299861742377204 7 0.208131535156347 8 0.20266746144102527 9 0.2006805255445446 10-11 0.2158309117552095 12-13 0.21616206773795626 14-15 0.200846103535918 16-17 0.20887663611752727 18-19 0.1963754977688366 20-21 0.19960426860061758 22-23 0.20117725951866475 24-25 0.19414019488529582 26-27 0.20349535139789218 28-29 0.19952147960493094 30-31 0.20366092938926558 32-33 0.20647575524261316 34-35 0.19794848868688375 36-37 0.19861080065237727 38-39 0.19289835994999543 40-41 0.19438856187235592 42-43 0.19165652501469504 44-45 0.20432324135475916 46-47 0.19397461689392245 48-49 0.200846103535918 50-51 0.20324698441083214 52-53 0.2044060303504458 54-55 0.21011847105282766 56-57 0.18768265322173378 58-59 0.18014885461424468 60-61 0.18809659820016722 62-63 0.18809659820016722 64-65 0.1857785063209398 66-67 0.19546481881628294 68-69 0.1874342862346737 70-71 0.1934778829198023 72-73 0.20233630545827846 74-75 0.19314672693705554 76-77 0.1971205987300168 78-79 0.19405740588960915 80-81 0.18528177234681967 82-83 0.20051494755317123 84-85 0.18536456134250634 86-87 0.18751707523036037 88-89 0.19173931401038175 90-91 0.18826217619154062 92-93 0.1972861767213902 94-95 0.19529924082490954 96-97 0.18470224937701282 98-99 0.19024911208802125 100-101 0.18271531348053216 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 603945.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.66300464198181 #Duplication Level Percentage of deduplicated Percentage of total 1 84.03206209570895 54.33765621370132 2 10.059942958416867 13.010122764363643 3 2.549967224774675 4.946656274775189 4 1.0931648326112706 2.8274929058237546 5 0.5454125916313134 1.7634008472225473 6 0.3482028292278071 1.3509504697625336 7 0.23860930552509216 1.0800436241552365 8 0.17399089179190502 0.900061907488201 9 0.13652662471034055 0.7945399590658976 >10 0.7395665952601825 8.746711741616494 >50 0.05281409926888492 2.301888822917398 >100 0.024099615524480544 2.9699897720973376 >500 0.0030765466626946456 1.3764903755345856 >1k 0.002563788885578871 3.593994321475886 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4923 0.8151404515311825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3734 0.6182682197882258 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2452 0.4059972348475441 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2317 0.3836442060121369 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2121 0.3511909197029531 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1687 0.2793300714469033 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1148 0.19008353409664788 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1123 0.1859440843123132 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1117 0.1849506163640729 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1057 0.1750159368816697 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 972 0.16094180761493182 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 936 0.1549809999254899 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 897 0.14852345826192784 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 826 0.13676742087441737 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 738 0.12219655763355934 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.6557799137338665E-4 0.0 22-23 0.0 0.0 0.0 3.311559827467733E-4 0.0 24-25 0.0 0.0 0.0 6.623119654935466E-4 0.0 26-27 0.0 0.0 0.0 7.4510096118024E-4 0.0 28-29 0.0 0.0 0.0 0.00223530288354072 0.0 30-31 0.0 0.0 0.0 0.006623119654935466 0.0 32-33 0.0 0.0 0.0 0.013329028305557626 0.0 34-35 0.0 0.0 0.0 0.01995214796049309 0.0 36-37 0.0 0.0 0.0 0.027899891546415652 0.0 38-39 0.0 0.0 0.0 0.03410906622291765 0.0 40-41 0.0 0.0 0.0 0.044374901688067625 0.0 42-43 0.0 0.0 0.0 0.05902855392461234 0.0 44-45 0.0 0.0 0.0 0.08088484878589938 0.0 46-47 0.0 0.0 0.0 0.13560837493480365 0.0 48-49 0.0 0.0 0.0 0.24397917028868524 0.0 50-51 0.0 0.0 0.0 0.3170818534800354 0.0 52-53 0.0 0.0 0.0 0.39374446348591347 0.0 54-55 0.0 0.0 0.0 0.5074965435594301 0.0 56-57 0.0 0.0 0.0 0.5929347871080977 0.0 58-59 0.0 0.0 0.0 0.7075975461341678 0.0 60-61 0.0 0.0 0.0 0.9214415219928966 0.0 62-63 0.0 0.0 0.0 1.0623483926516488 0.0 64-65 0.0 0.0 0.0 1.2229590442838338 0.0 66-67 0.0 0.0 0.0 1.5115614832476467 0.0 68-69 0.0 0.0 0.0 1.8062075188965883 0.0 70-71 0.0 0.0 0.0 2.1091324541141994 0.0 72-73 0.0 0.0 0.0 2.480109943786272 0.0 74-75 0.0 0.0 0.0 2.7853529708831104 0.0 76-77 0.0 0.0 0.0 3.026517315318448 0.0 78-79 0.0 0.0 0.0 3.3456688936906507 0.0 80-81 0.0 0.0 0.0 3.71722590633253 0.0 82-83 0.0 0.0 0.0 4.116931177507886 0.0 84-85 0.0 0.0 0.0 4.536091862669614 0.0 86-87 0.0 0.0 0.0 5.320765963788093 0.0 88-89 0.0 0.0 0.0 6.492726986728924 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 75 3.0754437E-4 31.66891 9 CCCTTAC 85 6.369941E-4 27.966373 1 TACCTGG 660 0.0 25.92384 2 GTACATG 5065 0.0 24.592741 1 TACATGG 5105 0.0 24.391937 2 GTAGCCC 295 0.0 24.166292 3 GAGTACT 3645 0.0 23.652956 12-13 GTGTAGC 350 0.0 23.092234 1 ACATGGG 5130 0.0 22.976034 3 ATGGGAT 1390 0.0 22.22672 5 GTCGGGA 150 3.0168329E-5 22.179285 2 CATGGGG 1100 0.0 22.037094 4 GTACCTG 790 0.0 21.66509 1 AGTACTT 3755 0.0 21.56854 12-13 GTACTTT 3980 0.0 21.480366 14-15 CGGGAGT 155 3.8828675E-5 21.465609 4 CATGGGA 3770 0.0 21.05484 4 TAGGACA 340 1.8189894E-12 20.969553 4 ATCGTAT 120 0.0047361488 19.809517 1 CTAGGAC 480 0.0 19.802935 3 >>END_MODULE