FastQCFastQC Report
Thu 26 May 2016
SRR937301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603945
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60871.0078732334898044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50900.8427919760905381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33100.5480631514459098No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29850.4942503042495591No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24360.4033479869855699No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20110.33297734065188056No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15480.2563147306460025No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12270.20316419541514544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8520.14107244865012544No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG8350.13825762279677786No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT8220.13610510890892383No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA8170.13527721895205688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7940.131468925150469No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG7720.1278262093402545No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG7610.12600485143514722No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC7510.12434907152141336No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7500.12418349353003999No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6770.11209630015978275No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA6570.10878474033231503No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG6330.10481086853935376No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC651.3274178E-436.538567
TACCTGG6400.026.7276732
ATAGCCC1806.661139E-926.39773
GTATACC950.001221135125.0166361
GTGTAGC2101.2532837E-924.8975091
AGTACTT47650.023.1270312-13
GTACTAC1252.2440487E-422.815171
CGGGAGT1703.1357813E-622.3604054
GAGTACT46950.022.25777812-13
GTCCTAA1954.1876592E-721.9376661
GTATAGG2858.367351E-1121.6810861
CGTTAAT550.002878789621.58917682-83
GTCGGGA1353.784523E-421.1181622
GTACTTT49900.020.98779714-15
GTACATG54300.020.745841
GTACCCG1150.003706944420.6659161
TACATGG54950.020.2342342
TAGGGTG2351.1256634E-720.2195155
ACCTGGG7550.020.1391743
TAGCCCT3102.564775E-1019.9260064
Produced by FastQC (version 0.11.5)