##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937301_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 603945 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40205316709303 33.0 31.0 34.0 31.0 34.0 2 32.60034605800197 34.0 31.0 34.0 31.0 34.0 3 32.66884236147331 34.0 31.0 34.0 31.0 34.0 4 36.21679623144492 37.0 35.0 37.0 35.0 37.0 5 36.16626182847776 37.0 35.0 37.0 35.0 37.0 6 36.27425179445148 37.0 37.0 37.0 35.0 37.0 7 36.237998493240276 37.0 37.0 37.0 35.0 37.0 8 36.23197807747394 37.0 37.0 37.0 35.0 37.0 9 37.9602993650084 39.0 38.0 39.0 35.0 39.0 10-11 37.89800230153408 39.0 38.0 39.0 35.0 39.0 12-13 37.83085877025226 39.0 38.0 39.0 35.0 39.0 14-15 39.02733278692596 40.0 38.0 41.0 36.0 41.0 16-17 39.01622912682446 40.0 38.0 41.0 36.0 41.0 18-19 39.09922509500038 40.0 38.5 41.0 36.0 41.0 20-21 39.04372252440206 40.0 38.5 41.0 36.0 41.0 22-23 38.93725090860923 40.0 38.0 41.0 35.0 41.0 24-25 38.800060435966856 40.0 38.0 41.0 35.0 41.0 26-27 38.71387874723692 40.0 38.0 41.0 35.0 41.0 28-29 38.61071289604186 40.0 38.0 41.0 35.0 41.0 30-31 38.62516371523897 40.0 38.0 41.0 35.0 41.0 32-33 38.48423449155138 40.0 38.0 41.0 35.0 41.0 34-35 38.35229863646524 40.0 38.0 41.0 35.0 41.0 36-37 38.177554247489425 40.0 38.0 41.0 34.0 41.0 38-39 38.01119803955658 40.0 37.0 41.0 34.0 41.0 40-41 37.81091490119133 40.0 37.0 41.0 33.5 41.0 42-43 37.55392295656061 40.0 37.0 41.0 33.0 41.0 44-45 37.13869226502413 40.0 37.0 41.0 33.0 41.0 46-47 36.584818981860934 40.0 37.0 41.0 31.0 41.0 48-49 36.089249848910086 40.0 36.0 41.0 29.5 41.0 50-51 35.79587545223489 40.0 36.0 41.0 28.5 41.0 52-53 35.54385333101524 39.5 36.0 41.0 27.5 41.0 54-55 35.320831367094684 39.0 35.0 41.0 27.0 41.0 56-57 35.10975006002202 39.0 35.0 40.5 26.0 41.0 58-59 34.89873746781578 39.0 35.0 40.0 26.0 41.0 60-61 34.574486087309275 38.5 35.0 40.0 24.5 41.0 62-63 34.20570912914255 38.0 34.0 40.0 23.0 41.0 64-65 33.869143713417614 37.5 34.0 40.0 22.0 41.0 66-67 33.46302229507654 37.0 34.0 39.5 20.5 41.0 68-69 33.06923312553295 36.5 33.0 39.0 19.0 41.0 70-71 32.66224656218695 36.0 33.0 39.0 17.5 40.0 72-73 32.20114662759026 35.5 32.5 38.5 12.0 40.0 74-75 31.753321080561975 35.0 32.0 37.5 7.5 39.5 76-77 30.431068226411348 34.0 30.5 36.0 7.0 39.0 78-79 30.975366962223383 35.0 31.5 36.0 3.5 39.0 80-81 30.805238887647054 35.0 32.0 36.0 2.0 38.5 82-83 30.47854937121758 35.0 31.0 36.0 2.0 37.0 84-85 30.12364950450786 34.5 31.0 35.0 2.0 37.0 86-87 29.768574125127287 34.0 31.0 35.0 2.0 36.5 88-89 29.386920994461416 34.0 30.5 35.0 2.0 36.0 90-91 28.955246752601646 34.0 30.0 35.0 2.0 36.0 92-93 28.652364867661788 34.0 29.0 35.0 2.0 35.0 94-95 28.308982606032004 34.0 29.0 35.0 2.0 35.0 96-97 27.882690476781825 34.0 29.0 35.0 2.0 35.0 98-99 27.48256298172847 34.0 29.0 35.0 2.0 35.0 100-101 25.737374264212804 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0096557498270613 1101 2 -0.06263843324104812 1101 3 -0.12873927309125577 1101 4 0.0054967990232412944 1101 5 -0.07740147916411644 1101 6 -0.03951593974935719 1101 7 -0.019589631634744364 1101 8 -0.08088200153187586 1101 9 0.04015389036003825 1101 10-11 -0.023754883360560086 1101 12-13 -0.13458687271884884 1101 14-15 -0.022902507888993284 1101 16-17 -0.04770588051412261 1101 18-19 -0.03588626167092457 1101 20-21 -0.04225184242488211 1101 22-23 -0.15275882532777985 1101 24-25 -0.026609049792867268 1101 26-27 -0.05934518712849979 1101 28-29 -0.0731162863695829 1101 30-31 -0.12113137516356431 1101 32-33 0.004688664505714257 1101 34-35 7.941494904031288E-4 1101 36-37 0.0452587163638043 1101 38-39 0.13682548006418216 1101 40-41 -0.01002067319532074 1101 42-43 0.3106403680241172 1101 44-45 0.2646047113470118 1101 46-47 0.11684542922888852 1101 48-49 0.26851094244739215 1101 50-51 0.16607278695257577 1101 52-53 0.3198545701972648 1101 54-55 0.24615914984078557 1101 56-57 0.2001037083108983 1101 58-59 0.018200610216389634 1101 60-61 0.0995785188477285 1101 62-63 0.25846021969689303 1101 64-65 0.3318337015388053 1101 66-67 0.4604016076373014 1101 68-69 0.3241354808974535 1101 70-71 0.17063718364004643 1101 72-73 0.2434463764746404 1101 74-75 0.27647201158856305 1101 76-77 0.21966040778407248 1101 78-79 0.26665489997520453 1101 80-81 0.3653604097948602 1101 82-83 0.43887621693901835 1101 84-85 0.4002157767784702 1101 86-87 0.2889240616172053 1101 88-89 0.12606366189215024 1101 90-91 0.20537294603111533 1101 92-93 0.1082912032497596 1101 94-95 -0.001050235092026952 1101 96-97 0.042067644686365924 1101 98-99 0.3498262938764505 1101 100-101 0.5170589842673401 1102 1 -0.004373146094991398 1102 2 0.04037752873250611 1102 3 0.005546797025189676 1102 4 -0.05138719126258451 1102 5 -0.08392949045581588 1102 6 0.10496171556334133 1102 7 -0.026812995480561597 1102 8 0.053736177267140306 1102 9 0.042208812833415266 1102 10-11 0.07388506931395256 1102 12-13 0.06384806511454855 1102 14-15 0.001196128389722162 1102 16-17 -0.0947200158899193 1102 18-19 -0.19500264500736364 1102 20-21 -0.07794314740443298 1102 22-23 -0.08304713717782874 1102 24-25 -0.08173390190071927 1102 26-27 -0.0036910368078082456 1102 28-29 -0.021473386230510982 1102 30-31 0.12462904993555668 1102 32-33 0.11994594495073585 1102 34-35 0.20250866056415617 1102 36-37 0.19506048899389583 1102 38-39 -0.04529461873814711 1102 40-41 0.12381393668075447 1102 42-43 0.15948421467292917 1102 44-45 0.26484341446260373 1102 46-47 0.109238064718852 1102 48-49 0.25220129261448676 1102 50-51 0.13374408238400548 1102 52-53 0.3106593110487097 1102 54-55 -0.028676352577377884 1102 56-57 0.2898249445854688 1102 58-59 0.46583122233420937 1102 60-61 0.2609210681969998 1102 62-63 0.07938099101930618 1102 64-65 0.1187289004926626 1102 66-67 0.6012312566399345 1102 68-69 0.679917285493012 1102 70-71 0.5862089294938713 1102 72-73 0.6150189110865973 1102 74-75 0.7057536189945885 1102 76-77 0.6199084514563644 1102 78-79 0.5384858566557789 1102 80-81 0.6426919165304419 1102 82-83 0.7506172967073752 1102 84-85 0.7663085181280742 1102 86-87 0.5388047100594129 1102 88-89 0.6567940934258694 1102 90-91 0.6129170023235382 1102 92-93 0.45616466984170145 1102 94-95 0.6011146983824531 1102 96-97 0.48040959208865175 1102 98-99 0.6698804691052942 1102 100-101 0.9369533321926973 1103 1 0.011062485668425381 1103 2 0.023013098606533333 1103 3 0.07620296872102728 1103 4 0.004003806716831093 1103 5 -0.05018404201346982 1103 6 -0.09267521141898527 1103 7 -0.0683273972187095 1103 8 -0.005466524886273305 1103 9 -0.03123238110719484 1103 10-11 -0.19030785772606862 1103 12-13 -0.09688600590876462 1103 14-15 -0.07192512890883052 1103 16-17 0.025009716320489872 1103 18-19 -0.015312327942289983 1103 20-21 0.01089368621932607 1103 22-23 -0.2289788936431023 1103 24-25 -0.19393725572835763 1103 26-27 -0.1370751141905231 1103 28-29 -0.13102939546671166 1103 30-31 -0.16892462009175802 1103 32-33 -0.13983736353505805 1103 34-35 -0.16597246645200414 1103 36-37 -0.22551261718442106 1103 38-39 -0.16976767391017944 1103 40-41 -0.09569406894698318 1103 42-43 -0.14952672217761176 1103 44-45 -0.3626644495538258 1103 46-47 -0.20523736375367463 1103 48-49 -0.07349486460552868 1103 50-51 -0.004817985980153594 1103 52-53 0.1823568502901196 1103 54-55 0.09445926253359715 1103 56-57 0.11187723324699306 1103 58-59 0.14104701566562738 1103 60-61 -0.14188768705277965 1103 62-63 -0.3507980817151335 1103 64-65 -0.08703559729203647 1103 66-67 0.2099480877986224 1103 68-69 0.39211723015970534 1103 70-71 -0.049948369191888276 1103 72-73 -0.07486020793376724 1103 74-75 0.2780599557069223 1103 76-77 0.2201882648965885 1103 78-79 0.07577552125990294 1103 80-81 -0.01251406890719764 1103 82-83 0.14865912711210783 1103 84-85 0.17857638377042306 1103 86-87 0.462113667892023 1103 88-89 0.20307083075395482 1103 90-91 0.22007610917581744 1103 92-93 0.24092139342824126 1103 94-95 0.37081604277195623 1103 96-97 0.4932549171455918 1103 98-99 0.7106225647689719 1103 100-101 1.0758897526523832 1104 1 0.10910158165948047 1104 2 0.07954341715232971 1104 3 0.05010336535808335 1104 4 0.008985043143958649 1104 5 -0.013653448767136922 1104 6 -0.05396685334989115 1104 7 0.014044611406887952 1104 8 0.04393378857756147 1104 9 -0.08065558628255332 1104 10-11 0.0032209606495641196 1104 12-13 0.03030949999043031 1104 14-15 -0.1278625521262171 1104 16-17 -0.14183881227659612 1104 18-19 -0.11122173170477367 1104 20-21 -0.11603780194031543 1104 22-23 -0.1896303307084466 1104 24-25 -0.13506740426503683 1104 26-27 -0.20468578416986816 1104 28-29 -0.16834441136118983 1104 30-31 -3.447150465731852E-4 1104 32-33 -0.01377728652733623 1104 34-35 -0.03120133015322324 1104 36-37 -0.15440333062750256 1104 38-39 -0.1590390917769966 1104 40-41 -0.06280402088965076 1104 42-43 0.11923723222820826 1104 44-45 0.22679101767362653 1104 46-47 0.14139198433615974 1104 48-49 -0.08531767054943629 1104 50-51 0.0038472898878652018 1104 52-53 -0.054324370418029844 1104 54-55 0.034882820241314505 1104 56-57 0.04771481807986078 1104 58-59 0.20471496986389326 1104 60-61 0.18577129969902018 1104 62-63 0.26980003292349863 1104 64-65 0.13858511146587915 1104 66-67 0.3453197499963139 1104 68-69 0.420626567688835 1104 70-71 0.12377606648926331 1104 72-73 0.18034727490889324 1104 74-75 0.253880966940649 1104 76-77 0.4038347314083026 1104 78-79 0.3642881551954922 1104 80-81 0.16963756884215186 1104 82-83 0.18609394296354864 1104 84-85 0.28233688152779735 1104 86-87 0.33258549658876646 1104 88-89 0.38440981274428054 1104 90-91 0.48282793707898364 1104 92-93 0.41291597877541264 1104 94-95 0.44166283770342574 1104 96-97 0.22984228183650046 1104 98-99 0.24142565179257502 1104 100-101 0.40312600579808944 1105 1 0.04787113800792753 1105 2 0.09063644250814917 1105 3 0.0838177344263471 1105 4 0.11726186170781006 1105 5 0.029871220537529553 1105 6 0.09620460115389307 1105 7 0.12158050571404289 1105 8 0.1862884921731336 1105 9 0.036378030840211295 1105 10-11 -0.03299171239676468 1105 12-13 -0.09173693280657602 1105 14-15 -0.0204042233271835 1105 16-17 0.004953333000123905 1105 18-19 0.026687426350584076 1105 20-21 0.021207997366452958 1105 22-23 0.07744160609071571 1105 24-25 0.015002173153447984 1105 26-27 0.12385508127596978 1105 28-29 0.3145992807106808 1105 30-31 0.06007765808751486 1105 32-33 -0.09801111296120268 1105 34-35 -0.47275398324091356 1105 36-37 -0.32112286532706946 1105 38-39 -0.08947209254339583 1105 40-41 -0.20598531810910004 1105 42-43 -0.3542492563233637 1105 44-45 -0.379397320641651 1105 46-47 -0.7544606921369379 1105 48-49 -1.0651645307671345 1105 50-51 -1.037226540898935 1105 52-53 -1.154256529540774 1105 54-55 -1.1809461003478106 1105 56-57 -0.9728238338547683 1105 58-59 -0.7882335150617763 1105 60-61 -1.2705315345678017 1105 62-63 -1.1310712476946136 1105 64-65 -1.475886528500503 1105 66-67 -1.30073262871786 1105 68-69 -1.1899424852941962 1105 70-71 -1.1200945218277454 1105 72-73 -0.8255094998015089 1105 74-75 -0.9729147903418252 1105 76-77 -0.943047482473375 1105 78-79 -0.9547503566581348 1105 80-81 -0.9171258193118597 1105 82-83 -0.8950627324166938 1105 84-85 -0.7723942671594948 1105 86-87 -0.6122515348055835 1105 88-89 -0.5374678282455001 1105 90-91 -0.8676639671658144 1105 92-93 -0.8818028520461105 1105 94-95 -0.6263852668039362 1105 96-97 -0.6940752162475157 1105 98-99 -0.5631081100419522 1105 100-101 -0.5565086722355908 1106 1 0.009726924626370703 1106 2 0.19760976981375222 1106 3 0.03055711055654342 1106 4 0.22087284724126732 1106 5 -0.07410707964330498 1106 6 0.0032457403943979557 1106 7 0.08326676249523501 1106 8 -0.021498578351284436 1106 9 -0.05745673407841423 1106 10-11 -0.23769899087778157 1106 12-13 -0.22593392647745958 1106 14-15 -0.14158980198902782 1106 16-17 0.14813540804533432 1106 18-19 -0.09514801668738926 1106 20-21 -0.03133212469501956 1106 22-23 0.038817058170280916 1106 24-25 0.21020165326194018 1106 26-27 0.20818204782207772 1106 28-29 0.16484792447198515 1106 30-31 -0.03750089073708551 1106 32-33 -0.22484768619808904 1106 34-35 -0.15330325991360638 1106 36-37 -0.14085613838312838 1106 38-39 0.19727349968083985 1106 40-41 -0.3787079672953624 1106 42-43 -0.3700579136470523 1106 44-45 0.050035884602472436 1106 46-47 -0.06462965687473599 1106 48-49 -0.13762440870564774 1106 50-51 -0.2952226982226236 1106 52-53 -0.5276601235733267 1106 54-55 -0.3991875108360574 1106 56-57 -0.3858267723719493 1106 58-59 -0.541398072023803 1106 60-61 -0.5562175653092041 1106 62-63 -0.3034548372063668 1106 64-65 -0.37017054748784517 1106 66-67 -0.3350765482476987 1106 68-69 -0.5817541941549536 1106 70-71 -0.36510751911526995 1106 72-73 -0.32767100522645976 1106 74-75 -0.5120055453147003 1106 76-77 -0.24473995534860293 1106 78-79 -0.4566716948136538 1106 80-81 -0.5122320580098751 1106 82-83 -0.36796621342935154 1106 84-85 -0.4520252572137444 1106 86-87 -0.7866412273914776 1106 88-89 -0.4474192296921551 1106 90-91 -0.5475775069488158 1106 92-93 -0.6153302028598553 1106 94-95 -1.0805557008003248 1106 96-97 -0.6458722325223825 1106 98-99 -0.36263907749222213 1106 100-101 0.10205823554017712 1107 1 0.16886154001098674 1107 2 0.2188322972862835 1107 3 0.17406672594115946 1107 4 0.18581103405445276 1107 5 0.0676511621149416 1107 6 0.12652870742736155 1107 7 0.08065687238534736 1107 8 0.09628988318718257 1107 9 0.05212430987762673 1107 10-11 0.21625018494638937 1107 12-13 0.3035475295664938 1107 14-15 0.2336505826263604 1107 16-17 0.19660724870467305 1107 18-19 0.3315003349609569 1107 20-21 0.26442680387641104 1107 22-23 0.12868656366478604 1107 24-25 -0.005028429155643721 1107 26-27 0.134023393975923 1107 28-29 0.0070182541423164935 1107 30-31 0.007914631240936387 1107 32-33 -0.2235084004838015 1107 34-35 0.05033684997650312 1107 36-37 0.0661493561223736 1107 38-39 -0.07702251680267835 1107 40-41 -0.5012697804821684 1107 42-43 -0.413172611449248 1107 44-45 -0.534356285727192 1107 46-47 -0.48858226676484406 1107 48-49 -0.6853064142001557 1107 50-51 -0.8576883575632834 1107 52-53 -0.6713242719249735 1107 54-55 -0.6611380602866106 1107 56-57 -0.5059160580862283 1107 58-59 -0.4172222478479739 1107 60-61 -0.4215678400344771 1107 62-63 -0.7486814855580164 1107 64-65 -0.6375640914438847 1107 66-67 -0.5325868504454974 1107 68-69 -0.38817589745166003 1107 70-71 -0.5867936454888962 1107 72-73 -0.44375960412756044 1107 74-75 -0.33176859476525067 1107 76-77 -0.2627859718321197 1107 78-79 -0.14142444206639837 1107 80-81 -0.051998541526355524 1107 82-83 -0.14767088375902304 1107 84-85 -0.24040094402693057 1107 86-87 -0.3700203482705966 1107 88-89 -0.4231403835383105 1107 90-91 -0.3099229739817879 1107 92-93 -0.4194854226400757 1107 94-95 -0.35232767882229865 1107 96-97 -0.13783651823666787 1107 98-99 0.22551339503525014 1107 100-101 0.5837890047709458 1108 1 0.031317194592624276 1108 2 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92-93 -0.6328421689865102 2316 94-95 -0.5280121318263404 2316 96-97 -0.9087741716485489 2316 98-99 -0.961888893397969 2316 100-101 -0.7552993982349818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 5.0 5 6.0 6 27.0 7 39.0 8 123.0 9 227.0 10 384.0 11 793.0 12 1442.0 13 2144.0 14 2791.0 15 4187.0 16 6421.0 17 10650.0 18 9745.0 19 4537.0 20 3129.0 21 2776.0 22 2860.0 23 2851.0 24 3180.0 25 3509.0 26 4085.0 27 5035.0 28 6252.0 29 7475.0 30 9603.0 31 12358.0 32 16246.0 33 22493.0 34 32211.0 35 49691.0 36 81340.0 37 137911.0 38 140020.0 39 17396.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.30232419438476 20.246286076867896 15.165165413919985 19.286224314827365 2 15.203702323887109 22.6093435660532 40.39920853720124 21.787745572858455 3 20.34258086415154 27.896414408596808 30.628120110274942 21.13288461697671 4 11.99645663098461 18.073003336396525 39.14926028032354 30.781279752295326 5 12.076629063135512 39.93779850424944 33.636170931565104 14.349401501049941 6 28.353740820770103 38.37501759266158 18.149169212428284 15.12207237414003 7 26.299083525817746 36.115209166397605 20.661980809510798 16.92372649827385 8 26.32789409631672 34.414392039010174 19.726961892225287 19.53075197244782 9 27.76544221742046 17.058010249277665 21.05125466722963 34.12529286607224 10-11 24.044573595277715 28.523458261927825 26.63206086646963 20.79990727632483 12-13 26.833365484204542 26.172851362796777 27.637026875092623 19.356756277906058 14-15 23.648097094934144 26.419458725546203 28.32906970005547 21.60337447946419 16-17 20.045947892606115 30.271133960873918 28.868771162937023 20.814146983582944 18-19 19.904958232951675 28.000231809187927 33.00838652526306 19.08642343259734 20-21 21.41958290903973 26.716754009057116 32.79934431115416 19.064318770748994 22-23 20.788940559148188 26.901834878335716 32.3100193480606 19.999205214455493 24-25 20.52455107667089 26.92538227818758 33.08579423623012 19.464272408911405 26-27 20.913295161391808 26.614166448651673 33.28054058887229 19.191997801084224 28-29 20.01274998033688 26.992122333577566 33.84589910129197 19.14922858479358 30-31 20.224606545298 27.347523367194032 33.18596892101102 19.241901166496948 32-33 19.943786271928733 27.524691817963554 32.841318331967315 19.690203578140395 34-35 20.396476500343574 26.765102782538147 33.202775087135414 19.63564562998286 36-37 20.864813848943196 26.855673943819387 32.97104868820836 19.30846351902905 38-39 20.8274760118885 26.851948439013483 33.17040458982192 19.150170959276096 40-41 20.2911688978301 27.270695179196778 33.25931997118943 19.17881595178369 42-43 20.110525418030846 27.894916393830847 32.38639845125306 19.60815973688525 44-45 20.558411775906745 27.769581667204797 31.684838851219897 19.98716770566856 46-47 20.505344029671576 28.15753090099264 30.89884012617043 20.438284943165357 48-49 20.981877488844184 28.275174063863428 30.493670781279754 20.24927766601263 50-51 21.353103345503317 28.667759481409732 30.24745630810753 19.731680864979424 52-53 20.896853190273948 29.095778589109933 29.874243515551914 20.133124705064205 54-55 20.293238622722267 28.895677586535196 30.203329773406516 20.607754017336017 56-57 21.067978044358345 29.24960054309581 29.40218066214639 20.280240750399457 58-59 21.006300242571758 28.676286747965463 29.65021649322372 20.667196516239063 60-61 20.727052960120538 28.801049764465308 30.1511727061239 20.32072456929025 62-63 21.407495715669473 28.385035061139675 30.164253367442402 20.043215855748453 64-65 21.038422372898193 28.514185894410915 30.288105704989693 20.1592860277012 66-67 20.692364370927816 28.665689756517565 30.007451009611803 20.634494862942816 68-69 20.74584606214142 29.146859399448626 29.732757121923353 20.3745374164866 70-71 20.85355454552981 29.308794675011796 29.301426454395678 20.53622432506271 72-73 20.885693966912523 28.67506865283151 29.302030236222038 21.13720714403393 74-75 20.661898020515114 29.361779632251285 28.99378254642393 20.982539800809676 76-77 20.984361158714783 28.652443517207693 29.478098171191085 20.885097152886438 78-79 20.817706910397472 28.474116020498556 29.547309771585162 21.160867297518816 80-81 20.742700080305326 29.451274536588596 29.026898144698603 20.779127238407472 82-83 20.631051885605288 29.498761474573804 28.93670605221676 20.933480587604148 84-85 20.621952804640888 28.769249747283034 28.721810985095285 21.886986462980794 86-87 20.587636291384147 29.068954954507447 28.773894973880072 21.569513780228334 88-89 21.036352648006027 28.003460580019702 29.15927774880163 21.80090902317264 90-91 20.490333264334506 28.078244669840615 29.514220658248806 21.91720140757607 92-93 20.369773974581765 28.4855278659263 29.317817353314464 21.82688080617747 94-95 20.08560382154004 28.49456490243317 29.14247158267723 22.27735969334956 96-97 19.758007765607797 28.468734735778924 29.189247365240213 22.584010133373074 98-99 19.959714807995443 28.58435550315923 28.764504656060826 22.691425032784494 100-101 20.03153085270753 29.454850766660094 28.210259781768077 22.303358598864293 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 440.0 1 312.0 2 238.5 3 301.5 4 274.0 5 274.0 6 431.5 7 832.5 8 1287.0 9 1519.5 10 1531.5 11 1473.5 12 1536.5 13 1666.0 14 1767.5 15 1861.5 16 1907.0 17 1966.5 18 2059.0 19 2129.5 20 2297.0 21 2494.0 22 2687.0 23 3027.5 24 3460.0 25 4066.0 26 4823.5 27 5723.0 28 6689.5 29 7842.5 30 9367.0 31 10561.0 32 11740.0 33 13378.0 34 15099.0 35 16498.5 36 18166.5 37 19629.5 38 20261.0 39 20544.5 40 20566.5 41 20759.5 42 20777.5 43 20932.5 44 21865.0 45 22091.5 46 21645.5 47 23264.5 48 31679.0 49 30302.0 50 21355.0 51 18491.0 52 15501.5 53 13895.0 54 12563.0 55 11266.0 56 9851.0 57 8507.5 58 7623.0 59 6878.5 60 5857.0 61 4647.0 62 3485.0 63 2389.5 64 1610.0 65 1157.5 66 807.0 67 578.5 68 391.0 69 264.5 70 209.5 71 170.5 72 136.0 73 127.0 74 91.0 75 77.5 76 67.5 77 39.0 78 27.5 79 19.0 80 12.0 81 11.0 82 8.0 83 1.5 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05596536108420469 2 0.0 3 0.0 4 0.0 5 0.016723377128712053 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035599268145278125 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014074129266737866 24-25 0.0 26-27 0.0018213579051072533 28-29 0.0037255048059012 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01688895512008544 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 8.278899568669333E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.6557799137338665E-4 84-85 0.0014074129266737866 86-87 0.0 88-89 0.0 90-91 0.011590459396137065 92-93 0.0014074129266737866 94-95 0.0 96-97 0.0 98-99 1.6557799137338665E-4 100-101 0.015233175206351573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 603945.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.66115316526777 #Duplication Level Percentage of deduplicated Percentage of total 1 82.81449162411462 51.06437052338316 2 10.482122756820825 12.926795536109353 3 2.9799074634483063 5.5123359156603176 4 1.238335888576771 3.05428875582321 5 0.61163771203355 1.8857143321677334 6 0.38071892723052436 1.4085340850926675 7 0.26501580545911163 1.1438826120141763 8 0.2005483334010981 0.9892833202307436 9 0.1313863365007559 0.7291289716916871 >10 0.798907939870769 9.109676304820052 >50 0.05577377225948275 2.3308913158128446 >100 0.035467767613340175 4.11219666952953 >500 0.00351970212956585 1.6103543794360102 >1k 0.001624477905953469 2.256599642010839 >5k 5.414926353178231E-4 1.8659476362177365 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6087 1.0078732334898044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5090 0.8427919760905381 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3310 0.5480631514459098 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2985 0.4942503042495591 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2436 0.4033479869855699 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2011 0.33297734065188056 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1548 0.2563147306460025 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1227 0.20316419541514544 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 852 0.14107244865012544 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 835 0.13825762279677786 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 822 0.13610510890892383 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 817 0.13527721895205688 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 794 0.131468925150469 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 772 0.1278262093402545 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 761 0.12600485143514722 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 751 0.12434907152141336 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 750 0.12418349353003999 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 677 0.11209630015978275 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 657 0.10878474033231503 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 633 0.10481086853935376 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.6557799137338665E-4 0.0 18-19 0.0 0.0 0.0 3.311559827467733E-4 0.0 20-21 0.0 0.0 0.0 4.139449784334666E-4 0.0 22-23 0.0 0.0 0.0 4.967339741201599E-4 0.0 24-25 0.0 0.0 0.0 9.106789525536265E-4 0.0 26-27 0.0 0.0 0.0 0.0010762569439270132 0.0 28-29 0.0 0.0 0.0 0.00273203685766088 0.0 30-31 0.0 0.0 0.0 0.006871486641995547 0.0 32-33 0.0 0.0 0.0 0.013494606296931012 0.0 34-35 0.0 0.0 0.0 0.020283303943239864 0.0 36-37 0.0 0.0 0.0 0.028479414516222504 0.0 38-39 0.0 0.0 0.0 0.03452301120135112 0.0 40-41 0.0 0.0 0.0 0.04412653470100754 0.0 42-43 0.0 0.0 0.0 0.05878018693755226 0.0 44-45 0.0 0.0 0.0 0.08080205979021268 0.0 46-47 0.0 0.0 0.0 0.13585674192186376 0.0 48-49 0.0 0.0 0.0 0.24530379421967233 0.0 50-51 0.0 0.0 0.0 0.31906878937651606 0.0 52-53 0.0 0.0 0.0 0.3967248673306344 0.0 54-55 0.0 0.0 0.0 0.5107253143912112 0.0 56-57 0.0 0.0 0.0 0.5969086589010588 0.0 58-59 0.0 0.0 0.0 0.7131444088451764 0.0 60-61 0.0 0.0 0.0 0.9289753206003858 0.0 62-63 0.0 0.0 0.0 1.0706272922203182 0.0 64-65 0.0 0.0 0.0 1.2329765127619237 0.0 66-67 0.0 0.0 0.0 1.523897043604964 0.0 68-69 0.0 0.0 0.0 1.8206955931417597 0.0 70-71 0.0 0.0 0.0 2.125110730281731 0.0 72-73 0.0 0.0 0.0 2.4985718898244045 0.0 74-75 0.0 0.0 0.0 2.8071264767487105 0.0 76-77 0.0 0.0 0.0 3.0505261240675887 0.0 78-79 0.0 0.0 0.0 3.3726581062845127 0.0 80-81 0.0 0.0 0.0 3.74752667875386 0.0 82-83 0.0 0.0 0.0 4.1536067025970915 0.0 84-85 0.0 0.0 0.0 4.577817516495708 0.0 86-87 0.0 0.0 0.0 5.367955691329509 0.0 88-89 0.0 0.0 0.0 6.556474513407678 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGTC 65 1.3274178E-4 36.53856 7 TACCTGG 640 0.0 26.727673 2 ATAGCCC 180 6.661139E-9 26.3977 3 GTATACC 95 0.0012211351 25.016636 1 GTGTAGC 210 1.2532837E-9 24.897509 1 AGTACTT 4765 0.0 23.12703 12-13 GTACTAC 125 2.2440487E-4 22.81517 1 CGGGAGT 170 3.1357813E-6 22.360405 4 GAGTACT 4695 0.0 22.257778 12-13 GTCCTAA 195 4.1876592E-7 21.937666 1 GTATAGG 285 8.367351E-11 21.681086 1 CGTTAAT 55 0.0028787896 21.589176 82-83 GTCGGGA 135 3.784523E-4 21.118162 2 GTACTTT 4990 0.0 20.987797 14-15 GTACATG 5430 0.0 20.74584 1 GTACCCG 115 0.0037069444 20.665916 1 TACATGG 5495 0.0 20.234234 2 TAGGGTG 235 1.1256634E-7 20.219515 5 ACCTGGG 755 0.0 20.139174 3 TAGCCCT 310 2.564775E-10 19.926006 4 >>END_MODULE