FastQCFastQC Report
Thu 26 May 2016
SRR937300_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937300_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779617
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57960.7434419721478623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43470.5575814791108967No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31220.40045304296853457No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29160.37402981207439034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28350.363640095072324No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21330.2735958810544152No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15950.20458763726291246No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13730.1761121165905823No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT12190.1563588274755425No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA12170.15610229125326924No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11630.14917581325189164No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.14160799469483093No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC10740.13775995136073227No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10630.13634900213822942No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8800.11287593780022756No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG8380.10748867713248941No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA250.001611719438.0204850-51
CATGGGG14300.027.2401774
GTACCCG1056.895828E-527.1470091
ACTAACG700.00838893527.143528
GTACATG65150.026.6157361
TACATGG66450.025.8805562
ACATGGG65900.025.1577683
ATGGGAT18850.024.6971475
GAGTACT43550.024.3785312-13
TACCTGG6950.023.2409652
TATAACG1252.252658E-422.8034882
ATGGGGG7600.022.5019955
GTACTTT47650.022.4795614-15
CATGGGA49100.022.4459844
AGTACTT45700.022.40006312-13
GTATAGG1954.211597E-721.926431
GTACCTG7800.021.3173621
GTATCAA114100.021.0680771
ACGTACA1150.003718913520.6553332
GTGTAGC5200.020.0992281
Produced by FastQC (version 0.11.5)