##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937300_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 779617 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.016808253283344 33.0 31.0 34.0 31.0 34.0 2 32.11007712761523 33.0 31.0 34.0 30.0 34.0 3 32.14884616420627 34.0 31.0 34.0 30.0 34.0 4 35.74353945591233 37.0 35.0 37.0 35.0 37.0 5 35.76898528379961 37.0 35.0 37.0 35.0 37.0 6 35.81906500243068 37.0 35.0 37.0 35.0 37.0 7 35.717262450664876 37.0 35.0 37.0 35.0 37.0 8 35.74044947711504 37.0 35.0 37.0 35.0 37.0 9 37.40150099343652 39.0 37.0 39.0 35.0 39.0 10-11 37.30796403875236 39.0 37.0 39.0 35.0 39.0 12-13 37.17458572606806 39.0 37.0 39.0 34.0 39.0 14-15 38.340894952265025 40.0 38.0 41.0 34.0 41.0 16-17 38.30611954331422 40.0 38.0 41.0 34.0 41.0 18-19 38.34469810176022 40.0 38.0 41.0 34.0 41.0 20-21 38.27696484299342 40.0 38.0 41.0 34.0 41.0 22-23 38.13440189221117 40.0 38.0 41.0 34.0 41.0 24-25 38.08031828449097 40.0 38.0 41.0 33.5 41.0 26-27 38.06787563637017 40.0 38.0 41.0 33.5 41.0 28-29 37.991990939140635 40.0 38.0 41.0 33.5 41.0 30-31 37.76465623504875 40.0 37.0 41.0 33.0 41.0 32-33 37.5176496920924 40.0 37.0 41.0 33.0 41.0 34-35 37.54209759407504 40.0 37.0 41.0 33.0 41.0 36-37 37.40615007112467 40.0 37.0 41.0 33.0 41.0 38-39 37.26168426291371 40.0 37.0 41.0 33.0 41.0 40-41 37.04363168068423 40.0 36.0 41.0 31.5 41.0 42-43 36.75881105722425 40.0 36.0 41.0 31.0 41.0 44-45 36.47075743602307 39.5 36.0 41.0 30.5 41.0 46-47 36.02837162350231 39.0 36.0 41.0 29.5 41.0 48-49 35.6076086078164 39.0 35.0 41.0 27.5 41.0 50-51 34.617271044628325 38.0 34.0 40.0 25.0 40.5 52-53 34.70765260377852 38.5 34.5 39.5 26.0 40.5 54-55 35.09223310933446 39.0 35.0 40.0 26.0 41.0 56-57 34.91996005731019 39.0 35.0 40.0 25.5 41.0 58-59 34.68172320511225 38.5 35.0 40.0 24.0 41.0 60-61 34.375639576869155 38.0 34.0 40.0 23.0 41.0 62-63 34.080131013048714 38.0 34.0 40.0 22.0 41.0 64-65 33.66449615644605 37.5 34.0 40.0 20.5 41.0 66-67 33.32289637091033 37.0 33.0 40.0 19.5 41.0 68-69 32.87660671842713 36.5 33.0 39.0 17.5 41.0 70-71 32.39358172025494 36.0 32.0 39.0 13.5 40.0 72-73 31.92023968179247 35.0 31.5 38.5 8.0 40.0 74-75 31.437552028752577 35.0 31.0 37.5 7.0 39.5 76-77 30.94177140826842 35.0 31.0 37.0 2.0 39.0 78-79 30.431656185024185 35.0 30.5 36.5 2.0 39.0 80-81 29.942410824802437 34.0 30.0 36.0 2.0 38.5 82-83 29.45828079685281 34.0 29.5 35.5 2.0 37.0 84-85 28.933728356359598 34.0 29.0 35.0 2.0 37.0 86-87 28.47224662879337 34.0 29.0 35.0 2.0 36.0 88-89 28.00067661428625 34.0 27.5 35.0 2.0 36.0 90-91 27.487604169739754 33.5 26.5 35.0 2.0 35.5 92-93 27.01568141792701 33.0 26.0 35.0 2.0 35.0 94-95 26.520461970429068 33.0 25.0 35.0 2.0 35.0 96-97 25.818678915416157 32.5 23.5 35.0 2.0 35.0 98-99 24.950876520137452 32.0 17.0 34.5 2.0 35.0 100-101 22.465363761949778 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.12985687862685324 1101 2 -0.1735353427712205 1101 3 -0.0717678032634197 1101 4 -0.09299301310976915 1101 5 -0.15379797680941465 1101 6 -0.28072177575311485 1101 7 -0.25884182503953923 1101 8 -0.30862883781120587 1101 9 -0.37235786896819434 1101 10-11 -0.37980138948430664 1101 12-13 -0.22746108556348332 1101 14-15 -0.3604022101785702 1101 16-17 -0.26467822177400535 1101 18-19 -0.2691276333867805 1101 20-21 -0.12688056389476543 1101 22-23 -0.2639990698817627 1101 24-25 -0.170732051699801 1101 26-27 -0.0581681447136404 1101 28-29 -0.34268392567509665 1101 30-31 -0.21870109630418 1101 32-33 -0.2858221363478819 1101 34-35 -0.2504339717866628 1101 36-37 -0.23289175965545894 1101 38-39 -0.13240385548753864 1101 40-41 -0.4347171365761682 1101 42-43 -0.2711364766487989 1101 44-45 -0.27025229854024957 1101 46-47 0.0661661414824053 1101 48-49 -0.16845746402902506 1101 50-51 -0.2780757656086834 1101 52-53 -0.1658892007441608 1101 54-55 -0.306979620484519 1101 56-57 -0.025043719199480563 1101 58-59 -0.04930924020104044 1101 60-61 -0.14802484791074733 1101 62-63 -0.13884272983460022 1101 64-65 -0.42231753839495667 1101 66-67 -0.5810040105465433 1101 68-69 -0.34300947301096585 1101 70-71 -0.2356660358241811 1101 72-73 -0.15229976816219448 1101 74-75 -0.35980656541063283 1101 76-77 -0.02551685589133612 1101 78-79 -0.14949252711531003 1101 80-81 -0.062042346320950514 1101 82-83 -0.03416806888158419 1101 84-85 -0.32494356495136856 1101 86-87 -0.5232088727370545 1101 88-89 -0.5597290755128519 1101 90-91 -0.5845326453881441 1101 92-93 -0.33077428826746313 1101 94-95 -0.3997788554081829 1101 96-97 -0.3065039178783273 1101 98-99 -0.44442474126582 1101 100-101 -0.0652724792463566 1102 1 -0.3084355215918322 1102 2 -0.23653104754912135 1102 3 -0.07868552070017643 1102 4 -0.12702609199017445 1102 5 -0.19945774702211594 1102 6 -0.1800223845973079 1102 7 -0.19976037175185013 1102 8 -0.2469124854895881 1102 9 -0.3168158984110221 1102 10-11 -0.503250927712493 1102 12-13 -0.20798994364395895 1102 14-15 -0.2566293608019521 1102 16-17 -0.3224803226054078 1102 18-19 -0.32757915620603484 1102 20-21 -0.5194003389271913 1102 22-23 -0.40566653392876617 1102 24-25 -0.4276023693052551 1102 26-27 -0.6190786999029925 1102 28-29 -0.63408435590609 1102 30-31 -0.5276664864535192 1102 32-33 -0.3965118171831108 1102 34-35 -0.27069850714428156 1102 36-37 -0.37015989917220793 1102 38-39 -0.3231146403224301 1102 40-41 -0.42982830911510206 1102 42-43 -0.44703016281407315 1102 44-45 -0.3828251259446276 1102 46-47 -0.19330806592153493 1102 48-49 -0.32141627667654404 1102 50-51 -0.1297430878991932 1102 52-53 -0.35919368662875684 1102 54-55 -0.2993712830756792 1102 56-57 -0.27591640768248027 1102 58-59 -0.300669216669462 1102 60-61 -0.27540104132332033 1102 62-63 -0.2060853709000412 1102 64-65 -0.20524092963581353 1102 66-67 -0.4437992184679018 1102 68-69 -0.2807136909757588 1102 70-71 -0.453478449441306 1102 72-73 -0.567617976076459 1102 74-75 -0.660432686173003 1102 76-77 -0.4803713959977962 1102 78-79 -0.5408529997314986 1102 80-81 -0.293803600015476 1102 82-83 -0.6322990093651129 1102 84-85 -0.4805642989665557 1102 86-87 -0.46650804275271796 1102 88-89 -0.5051386454944904 1102 90-91 -0.6227564009432243 1102 92-93 -0.5097653540637452 1102 94-95 -0.6053262304321017 1102 96-97 -0.628572038966734 1102 98-99 -0.8325859467631354 1102 100-101 -0.7908974482371214 1103 1 -0.005258269161181772 1103 2 -0.07995219180391189 1103 3 -0.13212063520399653 1103 4 0.09552846161466988 1103 5 -0.01792284751216755 1103 6 -0.04352630584165951 1103 7 -0.10390640219915781 1103 8 -0.23298902156416545 1103 9 -0.144048309388765 1103 10-11 -0.03833649004766215 1103 12-13 -0.18886354117892523 1103 14-15 -0.3082931695879765 1103 16-17 -0.25531323668620587 1103 18-19 -0.21403933011605147 1103 20-21 -0.20916125617631565 1103 22-23 -0.27895996573567317 1103 24-25 -0.17281668859289567 1103 26-27 -0.2601243578103336 1103 28-29 -0.4622345164986683 1103 30-31 -0.5441304902137389 1103 32-33 -0.482910844052995 1103 34-35 -0.47576903296530304 1103 36-37 -0.5020264288927123 1103 38-39 -0.817045089372364 1103 40-41 -0.5270303424173832 1103 42-43 -0.5559953851629871 1103 44-45 -0.4615889329898053 1103 46-47 -0.4189041951615735 1103 48-49 -0.3058052850064641 1103 50-51 -0.44500340891026724 1103 52-53 -0.43414777194736587 1103 54-55 -0.2551490255530737 1103 56-57 -0.2177821105035065 1103 58-59 -0.42647870773460284 1103 60-61 -0.3664241771002139 1103 62-63 -0.3412002011245221 1103 64-65 -0.44983072221461384 1103 66-67 -0.3491350049363433 1103 68-69 -0.3658854249404442 1103 70-71 -0.3494544954691463 1103 72-73 -0.26696167901935297 1103 74-75 -0.3023117717092241 1103 76-77 -0.2860842921479261 1103 78-79 -0.0021900091407438538 1103 80-81 0.01403366616781554 1103 82-83 0.08187230679772384 1103 84-85 -0.11630147984863726 1103 86-87 0.11354235459423734 1103 88-89 -0.029997854978596195 1103 90-91 0.0405725657806002 1103 92-93 -0.00537136257645443 1103 94-95 -0.310553431943152 1103 96-97 -0.3815630214103507 1103 98-99 -0.09899119713958981 1103 100-101 0.23996113454422385 1104 1 -0.045940882257411886 1104 2 -0.1162570103246594 1104 3 -0.25009816329388457 1104 4 -0.18550237640564404 1104 5 -0.0548913176542456 1104 6 -0.14183514726991575 1104 7 -0.15188533958785655 1104 8 -0.018511679245484913 1104 9 -0.09231551244310054 1104 10-11 -0.22041149036795105 1104 12-13 -0.1326382839818976 1104 14-15 -0.19932170148296535 1104 16-17 -0.21509761396830385 1104 18-19 -0.07234714659972497 1104 20-21 -0.22959096419522496 1104 22-23 -0.33867644043876055 1104 24-25 -0.17299541330559265 1104 26-27 -0.09952835253186976 1104 28-29 -0.06525233772271122 1104 30-31 -0.03481118153886342 1104 32-33 -0.24473422047670823 1104 34-35 -0.2765813015520351 1104 36-37 -0.24900027999389351 1104 38-39 -0.2291003595093244 1104 40-41 -0.0913001334568051 1104 42-43 -0.21627173584778348 1104 44-45 -0.3150052014227924 1104 46-47 -0.24955324132250922 1104 48-49 -0.17085019039127047 1104 50-51 -0.3969969261357491 1104 52-53 -0.34047424242255886 1104 54-55 -0.15709385791057429 1104 56-57 -0.2758971092863831 1104 58-59 -0.22224696776304143 1104 60-61 -0.28396738492053686 1104 62-63 -0.10276926317210666 1104 64-65 -0.06903177790754 1104 66-67 -0.357918139473135 1104 68-69 -0.39769842380019327 1104 70-71 -0.048529482977627936 1104 72-73 0.002430913193631312 1104 74-75 -0.22689314590368426 1104 76-77 -0.29245111981630245 1104 78-79 -0.35184665270345405 1104 80-81 -0.2988940880504707 1104 82-83 -0.197527588145455 1104 84-85 -0.04632787217180123 1104 86-87 0.13632238867364777 1104 88-89 0.09945008424219282 1104 90-91 0.04460572273805141 1104 92-93 0.10427208482467165 1104 94-95 0.2742485615740726 1104 96-97 0.1196246331320765 1104 98-99 0.052871639650792446 1104 100-101 0.36504216922578436 1105 1 0.15730656627269468 1105 2 0.1301194280537814 1105 3 0.06208925623146655 1105 4 0.11006287752151422 1105 5 0.20832685504603887 1105 6 0.0369927352472601 1105 7 0.060300724575000686 1105 8 0.165408300153004 1105 9 0.18962292699252714 1105 10-11 0.058095171238697674 1105 12-13 0.10932306107984147 1105 14-15 0.246892771077718 1105 16-17 0.03233532947241713 1105 18-19 0.13196697221135878 1105 20-21 0.08542509685030097 1105 22-23 0.17502424130857008 1105 24-25 0.24062259132974617 1105 26-27 0.18344422848492314 1105 28-29 0.026762544157449497 1105 30-31 -0.09276377095890354 1105 32-33 0.005359977216464529 1105 34-35 0.24017233420784834 1105 36-37 0.10363590005245982 1105 38-39 0.002199704192712204 1105 40-41 -0.01166377721333589 1105 42-43 -0.09487395051009173 1105 44-45 0.19981433206143606 1105 46-47 0.14705360867952066 1105 48-49 0.3539997032127715 1105 50-51 0.176242119146913 1105 52-53 0.30592523711824526 1105 54-55 0.4116026899811587 1105 56-57 0.5292878299256074 1105 58-59 0.5430137362122309 1105 60-61 0.5795205049155996 1105 62-63 0.5575870616269611 1105 64-65 0.5348838643309719 1105 66-67 0.501399977657293 1105 68-69 0.3734716606388986 1105 70-71 0.27571795199884974 1105 72-73 0.37301683639878647 1105 74-75 0.4355667014825002 1105 76-77 0.46635060420478425 1105 78-79 0.5657168121452223 1105 80-81 0.5592014921124395 1105 82-83 0.6989701551759957 1105 84-85 0.4599467784835163 1105 86-87 0.4802341003609385 1105 88-89 0.5741576647846074 1105 90-91 0.7721493992943422 1105 92-93 0.8773911805268142 1105 94-95 0.8172426928544851 1105 96-97 0.8422713850058621 1105 98-99 1.0085451870410438 1105 100-101 0.4343601508199626 1106 1 -0.10621514102757601 1106 2 -0.06804088605768044 1106 3 -0.24765848380957323 1106 4 -0.03721612067948854 1106 5 0.05305890393132273 1106 6 -0.05401116231087855 1106 7 -0.35357893971916354 1106 8 -0.4363519287344175 1106 9 -0.47239967019412177 1106 10-11 -0.694246487458237 1106 12-13 -0.31518077320104254 1106 14-15 -0.31402319513383503 1106 16-17 -0.41066068005601153 1106 18-19 -0.44334256567249497 1106 20-21 -0.22702594337960846 1106 22-23 -0.17554693598466287 1106 24-25 -0.4520358060417706 1106 26-27 -0.7809002432461583 1106 28-29 -0.346283270172691 1106 30-31 -0.39426624517943054 1106 32-33 -0.19173423539829315 1106 34-35 -0.23650639518499617 1106 36-37 -0.5583106042399777 1106 38-39 -0.33676930917881265 1106 40-41 -0.4461421884577774 1106 42-43 -0.4985739875152717 1106 44-45 -0.1712610767976983 1106 46-47 -0.4549212822673496 1106 48-49 -0.07602126638690976 1106 50-51 -0.5066026978056186 1106 52-53 -0.2209299476913742 1106 54-55 -0.39554827992289887 1106 56-57 -0.39424842313212594 1106 58-59 -0.15888394047145482 1106 60-61 -0.24127672434187986 1106 62-63 -0.36267719697994494 1106 64-65 -0.3271901556325929 1106 66-67 -0.3691772229892578 1106 68-69 -0.15852179697416346 1106 70-71 -0.17002701345497684 1106 72-73 -0.26360755465780983 1106 74-75 0.030439321977599576 1106 76-77 -0.07716171563527396 1106 78-79 0.13854011483314466 1106 80-81 -0.4349185296100764 1106 82-83 -0.4124506673641619 1106 84-85 -0.674457302017963 1106 86-87 -0.7564652622208996 1106 88-89 -0.31096369136698243 1106 90-91 0.2060543104098329 1106 92-93 0.1754182119267469 1106 94-95 0.29770604251174504 1106 96-97 0.5223015658506398 1106 98-99 0.5508624479531647 1106 100-101 0.7554945917454425 1107 1 0.1224023799400058 1107 2 -0.013964573856824813 1107 3 0.010161243990637558 1107 4 0.05778998465309826 1107 5 0.10763790336980605 1107 6 0.12635729673883134 1107 7 0.23079319144298438 1107 8 0.10022748069720677 1107 9 0.15687383979278025 1107 10-11 0.22554281172087798 1107 12-13 0.10330546083373093 1107 14-15 0.4787003179725744 1107 16-17 0.27189054680388836 1107 18-19 0.2736222373079755 1107 20-21 0.05068201672627737 1107 22-23 0.2369400289074477 1107 24-25 0.35609364483482153 1107 26-27 0.30494340676230536 1107 28-29 0.155270247301047 1107 30-31 0.0453034402845347 1107 32-33 0.2981433905631903 1107 34-35 0.2912340969850291 1107 36-37 0.12332081192979416 1107 38-39 0.08385869335245388 1107 40-41 0.19583130459461984 1107 42-43 0.06530205195102212 1107 44-45 0.08047025291700294 1107 46-47 0.2022264133038334 1107 48-49 0.26492011350737954 1107 50-51 0.4493381918794839 1107 52-53 0.34042463398193945 1107 54-55 0.3450553012780304 1107 56-57 0.22685909311162078 1107 58-59 0.322448002450372 1107 60-61 0.32990788843742536 1107 62-63 0.7192162582817829 1107 64-65 0.7748713968153353 1107 66-67 0.5941631515305588 1107 68-69 0.5365297641192726 1107 70-71 0.7176419973028914 1107 72-73 0.912053113631 1107 74-75 0.8663841333361937 1107 76-77 0.7550075937045477 1107 78-79 1.0561127772037935 1107 80-81 1.2148341375735363 1107 82-83 1.1334881723552606 1107 84-85 1.1730635764369026 1107 86-87 0.9185296128648872 1107 88-89 1.0801679788202776 1107 90-91 1.209615902965787 1107 92-93 1.034028513449961 1107 94-95 1.4831958506171325 1107 96-97 1.5144591101700051 1107 98-99 1.75306393284135 1107 100-101 1.9215801377047832 1108 1 -0.08406331118846211 1108 2 -0.07930729582843554 1108 3 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-2.767234850556605 2316 60-61 -2.993301305754791 2316 62-63 -2.9293646953524686 2316 64-65 -2.7867375868228663 2316 66-67 -2.6460175740035012 2316 68-69 -2.331685351904767 2316 70-71 -2.3431905683855803 2316 72-73 -2.3744582694457144 2316 74-75 -2.754421235791323 2316 76-77 -2.2963763603244907 2316 78-79 -2.2838617808520887 2316 80-81 -2.582433046705095 2316 82-83 -2.527923416888939 2316 84-85 -2.532437147342545 2316 86-87 -2.2379120241926245 2316 88-89 -1.971211588666673 2316 90-91 -1.7481680159709185 2316 92-93 -1.3353621958538966 2316 94-95 -0.9523395268448134 2316 96-97 -1.429967788106083 2316 98-99 -1.4262096557887318 2316 100-101 -0.3684931175481019 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2647.0 3 546.0 4 394.0 5 392.0 6 407.0 7 551.0 8 617.0 9 784.0 10 1103.0 11 1581.0 12 2413.0 13 3590.0 14 4026.0 15 5521.0 16 7528.0 17 11209.0 18 9393.0 19 5088.0 20 4586.0 21 4734.0 22 5051.0 23 5475.0 24 6089.0 25 7179.0 26 8123.0 27 9594.0 28 11215.0 29 13619.0 30 16965.0 31 21480.0 32 27504.0 33 36817.0 34 50810.0 35 72525.0 36 107747.0 37 157782.0 38 136573.0 39 17956.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.88496112231044 19.4645575348665 14.752694697000864 20.89778664582219 2 14.5327389903882 21.257528900953982 41.52058735067519 22.689144757982625 3 19.393900448534232 27.433330334537136 30.46292845300672 22.709840763921914 4 11.496229574990135 16.915179254243736 39.83450488349406 31.75408628727207 5 12.22767759597959 39.25259951415755 34.22528694266288 14.294435947199979 6 28.389836501016326 37.49136992271822 19.11563725004918 15.003156326216278 7 26.53489430526378 34.857149469147835 21.350696703291053 17.257259522297332 8 25.585368550147454 34.01912362479849 20.769881447924245 19.62562637712981 9 27.07866540688893 16.768541550169363 21.497207278430164 34.65558576451155 10-11 23.60807207633427 27.669051041214026 27.887475862707333 20.83540101974437 12-13 26.12349691508414 25.408932949524115 28.243982839151112 20.223587296240634 14-15 22.984124168400406 25.863574925428388 28.54044027618972 22.611860629981486 16-17 20.030160366758288 29.654544239061405 29.039123626649115 21.276171767531196 18-19 19.87089708081884 28.05452840412055 32.40453704911837 19.670037465942244 20-21 21.470164419941202 26.700019410062637 31.715142548271285 20.114673621724872 22-23 20.601490035579868 26.9297708903194 31.450458116529212 21.01828095757152 24-25 20.43894786698791 26.89720501864028 32.13640532146045 20.52744179291136 26-27 20.64747715930162 26.680378048506153 32.30187321042365 20.370271581768577 28-29 19.930785972589497 26.959660717568457 32.80229393593074 20.3072593739113 30-31 20.048041199474465 27.32004154914549 32.381336823187304 20.25058042819274 32-33 19.775759878077547 27.454277473850112 31.892799774769998 20.87716287330234 34-35 20.399566479562058 26.90982980594432 31.99424544045008 20.69635827404354 36-37 20.71734220772293 26.760577863642382 32.0841190330206 20.437960895614093 38-39 20.711697068904243 26.908599196257516 32.1684658647237 20.211237870114545 40-41 20.28440727811168 27.07049064015095 32.42348368388851 20.221618397848854 42-43 19.868386464848108 27.651760792174912 31.667136913939608 20.812715829037373 44-45 20.23269417428922 27.795126278371658 31.00289903515482 20.969280512184305 46-47 20.366922938469052 27.71997377285233 30.379329407832145 21.533773880846468 48-49 20.516021532610694 27.8694826542144 30.23237425703622 21.382121556138685 50-51 20.98948124939332 28.13635612685242 30.097910585155724 20.776252038598535 52-53 20.541143173473017 28.41724976644933 30.02648300657671 21.015124053500944 54-55 20.07814338114762 28.16337340785111 30.380135793088463 21.378347417912806 56-57 20.71169935589852 28.779549167526795 29.232643900959275 21.276107575615406 58-59 20.718854244115096 28.105321940207297 29.72034061137055 21.45548320430706 60-61 20.676258095941282 27.933383070478307 30.205483809402633 21.184875024177778 62-63 21.113316042331252 27.840291290835857 30.055433170448325 20.99095949638457 64-65 20.682198916208232 28.076414195320275 30.217458776265048 21.02392811220644 66-67 20.440624598380673 28.165659940881632 30.01593625498008 21.377779205757616 68-69 20.345528050376867 28.535496503784113 29.87935950584502 21.239615939993996 70-71 20.376206370484425 28.398305008501946 29.48781387481894 21.73767474619469 72-73 20.438616672471873 28.00952910979812 29.435875157608187 22.11597906012182 74-75 20.253960134209812 28.449216006210516 29.523331518934548 21.773492340645127 76-77 20.514727195486177 27.944105490539105 29.80652741027719 21.734639903697527 78-79 20.356128130034904 27.83055154423517 30.005854706869417 21.807465618860512 80-81 20.158630599691016 28.768215784914286 29.430654701465485 21.642498913929213 82-83 20.197018059881717 28.504833555148412 29.454450578722074 21.843697806247793 84-85 20.290742659136654 28.03421923238077 29.167898391089427 22.507139717393148 86-87 20.341427393497547 28.02826740668523 29.47428561880261 22.156019581014615 88-89 20.791668808801603 27.24988432471338 29.62315562181893 22.335291244666085 90-91 20.306235643597446 27.398089626370403 30.046351818788295 22.24932291124385 92-93 20.199643076788618 27.709682747062004 29.945240499024152 22.145433677125233 94-95 19.74799231176055 27.694627036204256 29.891147535926972 22.666233116108224 96-97 19.508553778682103 27.584562516589234 29.778293922989963 23.128589781738697 98-99 19.749493536809847 27.665230402188335 29.502637721995413 23.082638339006404 100-101 20.20502743252396 27.540351963973364 29.794072929772998 22.460547673729682 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1121.0 1 675.0 2 250.0 3 275.5 4 237.5 5 243.0 6 440.5 7 872.5 8 1396.5 9 1677.0 10 1562.5 11 1405.5 12 1425.5 13 1529.5 14 1711.0 15 1813.0 16 1854.0 17 1906.0 18 2025.5 19 2177.0 20 2321.5 21 2659.5 22 3021.5 23 3397.0 24 4001.0 25 4711.5 26 5733.0 27 7178.0 28 8353.0 29 9861.0 30 11751.0 31 13213.5 32 14956.0 33 17151.0 34 19644.5 35 21740.0 36 23891.5 37 26210.5 38 26929.0 39 26634.0 40 26598.5 41 26863.0 42 27018.5 43 27499.0 44 28524.0 45 29141.0 46 29009.0 47 30675.0 48 39710.0 49 39148.0 50 29120.5 51 24571.0 52 20737.0 53 18489.0 54 16941.5 55 14938.0 56 13001.0 57 11294.5 58 10009.0 59 8879.0 60 7544.5 61 6208.0 62 4796.5 63 3379.0 64 2278.0 65 1609.0 66 1161.0 67 816.0 68 556.5 69 411.0 70 317.5 71 229.0 72 174.5 73 144.5 74 112.5 75 91.5 76 69.5 77 49.0 78 30.0 79 19.0 80 16.0 81 11.0 82 6.5 83 5.5 84 5.0 85 3.0 86 2.0 87 2.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22998472326796363 2 0.2071529994856449 3 0.1958654057056221 4 0.20368976048495607 5 0.20356149237381946 6 0.23267835360183267 7 0.23229354926842283 8 0.22203210037749305 9 0.218312325154531 10-11 0.22767589726750445 12-13 0.23088260004591998 14-15 0.21465668398713728 16-17 0.22767589726750445 18-19 0.2194026040991923 20-21 0.2142077455981591 22-23 0.21151411526429 24-25 0.20490830754075398 26-27 0.2147849520982739 28-29 0.22318651337772266 30-31 0.2235713177111325 32-33 0.22389198798897408 34-35 0.23075433193478337 36-37 0.2113858471531534 38-39 0.20926942331939916 40-41 0.20689646326337163 42-43 0.21260439420895133 44-45 0.22729109293409458 46-47 0.2304977957125101 48-49 0.23344796226865244 50-51 0.23287075576853764 52-53 0.24993041454970838 54-55 0.23293488982410593 56-57 0.2095900935972407 58-59 0.19823836576164963 60-61 0.1958012716500538 62-63 0.2004830577065405 64-65 0.19439032242755097 66-67 0.19458272459425588 68-69 0.20029065553983558 70-71 0.20009825337313067 72-73 0.2026636155958631 74-75 0.202856017762568 76-77 0.2118347855421316 78-79 0.20657579298553008 80-81 0.20304841992927297 82-83 0.20093199609551868 84-85 0.20202227504018 86-87 0.20760193787462305 88-89 0.20227881126245323 90-91 0.21215545581997317 92-93 0.20247121342915814 94-95 0.19823836576164963 96-97 0.20875635087485267 98-99 0.21459254993156898 100-101 0.1957371375944855 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 779617.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.250229344788025 #Duplication Level Percentage of deduplicated Percentage of total 1 82.22116488975747 51.18286371383035 2 11.0073809706846 13.704239798211438 3 3.008817530155447 5.618987439263857 4 1.2338671061651922 3.072340413590925 5 0.6609423930557136 2.0571907775705602 6 0.36798372523511325 1.3744242774621156 7 0.2522927072225459 1.0993695220634656 8 0.18748431613948305 0.9336753342586851 9 0.14791557320540413 0.828700052013197 >10 0.820152113860253 9.4172654876166 >50 0.05843207260563721 2.504770789440206 >100 0.028179369512038418 3.220582795223073 >500 0.002486414954357474 0.9926550994078066 >1k 0.00269361620055393 3.245347577468792 >5k 2.0720124619645616E-4 0.7475869225789562 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5796 0.7434419721478623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4347 0.5575814791108967 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3122 0.40045304296853457 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2916 0.37402981207439034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2835 0.363640095072324 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2133 0.2735958810544152 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1595 0.20458763726291246 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1373 0.1761121165905823 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1219 0.1563588274755425 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1217 0.15610229125326924 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1163 0.14917581325189164 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1104 0.14160799469483093 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1074 0.13775995136073227 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1063 0.13634900213822942 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 880 0.11287593780022756 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 838 0.10748867713248941 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.413405556831111E-5 0.0 22-23 0.0 0.0 0.0 1.2826811113662222E-4 0.0 24-25 0.0 0.0 0.0 5.130724445464889E-4 0.0 26-27 0.0 0.0 0.0 0.0010261448890929777 0.0 28-29 0.0 0.0 0.0 0.003206702778415555 0.0 30-31 0.0 0.0 0.0 0.0075678185570607105 0.0 32-33 0.0 0.0 0.0 0.013275749502640398 0.0 34-35 0.0 0.0 0.0 0.01911194855935671 0.0 36-37 0.0 0.0 0.0 0.02719283956096391 0.0 38-39 0.0 0.0 0.0 0.03456825595131968 0.0 40-41 0.0 0.0 0.0 0.04425249834213466 0.0 42-43 0.0 0.0 0.0 0.060221878178644134 0.0 44-45 0.0 0.0 0.0 0.08401561279448755 0.0 46-47 0.0 0.0 0.0 0.13480978480458994 0.0 48-49 0.0 0.0 0.0 0.2360774585469532 0.0 50-51 0.0 0.0 0.0 0.30970335433937435 0.0 52-53 0.0 0.0 0.0 0.38454779718759335 0.0 54-55 0.0 0.0 0.0 0.4806847464844918 0.0 56-57 0.0 0.0 0.0 0.5626480695007934 0.0 58-59 0.0 0.0 0.0 0.6764218840789773 0.0 60-61 0.0 0.0 0.0 0.864591203116402 0.0 62-63 0.0 0.0 0.0 0.9963866873092814 0.0 64-65 0.0 0.0 0.0 1.1493464098397033 0.0 66-67 0.0 0.0 0.0 1.3965831940555427 0.0 68-69 0.0 0.0 0.0 1.6709486837767775 0.0 70-71 0.0 0.0 0.0 1.9565376332224669 0.0 72-73 0.0 0.0 0.0 2.2792602008422085 0.0 74-75 0.0 0.0 0.0 2.5652339546213074 0.0 76-77 0.0 0.0 0.0 2.794449069222452 0.0 78-79 0.0 0.0 0.0 3.0882471777808846 0.0 80-81 0.0 0.0 0.0 3.441689958017847 0.0 82-83 0.0 0.0 0.0 3.843425682097748 0.0 84-85 0.0 0.0 0.0 4.2498431922341355 0.0 86-87 0.0 0.0 0.0 4.9283173660912984 0.0 88-89 0.0 0.0 0.0 5.858902512387493 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGTA 25 0.0016117194 38.02048 50-51 CATGGGG 1430 0.0 27.240177 4 GTACCCG 105 6.895828E-5 27.147009 1 ACTAACG 70 0.008388935 27.14352 8 GTACATG 6515 0.0 26.615736 1 TACATGG 6645 0.0 25.880556 2 ACATGGG 6590 0.0 25.157768 3 ATGGGAT 1885 0.0 24.697147 5 GAGTACT 4355 0.0 24.37853 12-13 TACCTGG 695 0.0 23.240965 2 TATAACG 125 2.252658E-4 22.803488 2 ATGGGGG 760 0.0 22.501995 5 GTACTTT 4765 0.0 22.47956 14-15 CATGGGA 4910 0.0 22.445984 4 AGTACTT 4570 0.0 22.400063 12-13 GTATAGG 195 4.211597E-7 21.92643 1 GTACCTG 780 0.0 21.317362 1 GTATCAA 11410 0.0 21.068077 1 ACGTACA 115 0.0037189135 20.655333 2 GTGTAGC 520 0.0 20.099228 1 >>END_MODULE