FastQCFastQC Report
Thu 26 May 2016
SRR937300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937300_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779617
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74540.956110500412382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62010.7953905571581944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.5196141182144566No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35770.45881503353569764No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31420.403018405191267No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23000.29501665561423107No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT19410.24896840371618373No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.20125266637336026No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT11410.14635391480688595No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA10420.13365537180436035No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC10400.1333988355820871No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10050.1289094516923053No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9610.12326565480229394No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG9020.11569783624523323No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG8910.11428688702273039No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8650.11095191613317822No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8500.10902789446612887No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG8430.10813001768817253No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8370.1073604090213528No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG500.00161571737.9989475
GGTCGGG1751.5097612E-1029.881231
GTCGGGA1503.3345714E-828.4992122
TACCTGG7300.027.328012
GTGAGCG908.9430506E-426.389859
GTACCCG1151.2742399E-424.80261
GTGCTAG3100.023.0024151
GAGTACT57150.022.64955712-13
GGACGTG1050.002195945722.6184226
TTGCGCG1205.9335434E-921.7702318-19
AGTACTT58300.021.75465212-13
CGGCAAT550.00287721321.59169690-91
TACCCGG1100.002877007221.5903112
CGGTATA1100.002877007221.5903112
GTACTTT60950.021.23608214-15
AGCGACT1353.7935007E-421.111888
GTACCTG9300.020.9577521
GTGTAGC4800.020.7980161
CGGGAGT2108.602674E-720.3565794
GCGGTAT1456.110325E-419.671031
Produced by FastQC (version 0.11.5)