##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937300_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 779617 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41885053814886 33.0 31.0 34.0 31.0 34.0 2 32.61072552291702 34.0 31.0 34.0 31.0 34.0 3 32.65185982347742 34.0 31.0 34.0 31.0 34.0 4 36.23971385949768 37.0 35.0 37.0 35.0 37.0 5 36.2025327821225 37.0 35.0 37.0 35.0 37.0 6 36.2733573023677 37.0 37.0 37.0 35.0 37.0 7 36.22444482354797 37.0 36.0 37.0 35.0 37.0 8 36.216437045369716 37.0 36.0 37.0 35.0 37.0 9 37.94954702116552 39.0 38.0 39.0 35.0 39.0 10-11 37.87905984605261 39.0 38.0 39.0 35.0 39.0 12-13 37.790448386836104 39.0 37.5 39.0 35.0 39.0 14-15 38.96912843101164 40.0 38.0 41.0 36.0 41.0 16-17 38.957283512288726 40.0 38.0 41.0 36.0 41.0 18-19 39.02942085665141 40.0 38.0 41.0 36.0 41.0 20-21 38.983718287312875 40.0 38.0 41.0 35.5 41.0 22-23 38.8409545969367 40.0 38.0 41.0 35.0 41.0 24-25 38.78197243005219 40.0 38.0 41.0 35.0 41.0 26-27 38.70509108959912 40.0 38.0 41.0 35.0 41.0 28-29 38.57010301212005 40.0 38.0 41.0 35.0 41.0 30-31 38.58892250938602 40.0 38.0 41.0 35.0 41.0 32-33 38.474568281604945 40.0 38.0 41.0 35.0 41.0 34-35 38.32837021255308 40.0 38.0 41.0 34.5 41.0 36-37 38.14133799032089 40.0 37.5 41.0 34.0 41.0 38-39 37.98807427236707 40.0 37.0 41.0 34.0 41.0 40-41 37.78159403912434 40.0 37.0 41.0 33.0 41.0 42-43 37.527741827076625 40.0 37.0 41.0 33.0 41.0 44-45 37.12317779114616 40.0 37.0 41.0 32.5 41.0 46-47 36.57061544322404 40.0 36.5 41.0 31.0 41.0 48-49 36.08324215608433 40.0 36.0 41.0 30.0 41.0 50-51 35.78534267467231 40.0 36.0 41.0 28.5 41.0 52-53 35.52833634977175 39.0 35.5 41.0 27.5 41.0 54-55 35.30613172878478 39.0 35.0 41.0 27.0 41.0 56-57 35.08242957760028 39.0 35.0 40.0 26.0 41.0 58-59 34.84659005639949 39.0 35.0 40.0 26.0 41.0 60-61 34.5268869201159 38.0 34.5 40.0 24.5 41.0 62-63 34.167961319468404 38.0 34.0 40.0 23.0 41.0 64-65 33.782086588671106 37.5 34.0 40.0 22.0 41.0 66-67 33.380137298186156 37.0 33.5 39.5 20.5 41.0 68-69 32.961525338724016 36.0 33.0 39.0 19.5 40.5 70-71 32.55248666973655 36.0 33.0 39.0 17.5 40.0 72-73 32.08274896519701 35.0 32.0 38.0 14.0 40.0 74-75 31.610022613667994 35.0 32.0 37.5 9.5 39.5 76-77 30.333078934912912 34.0 30.0 36.0 7.5 38.5 78-79 30.877055656816104 35.0 31.0 36.0 6.5 39.0 80-81 30.67174330472527 35.0 31.0 36.0 2.0 38.0 82-83 30.33537621678337 34.5 31.0 36.0 2.0 37.0 84-85 29.977571679427207 34.0 31.0 35.0 2.0 37.0 86-87 29.585946047867097 34.0 30.0 35.0 2.0 36.0 88-89 29.132408605764113 34.0 29.5 35.0 2.0 36.0 90-91 28.65171808721462 34.0 29.0 35.0 2.0 35.5 92-93 28.35094604145369 34.0 29.0 35.0 2.0 35.0 94-95 27.984865004226435 34.0 29.0 35.0 2.0 35.0 96-97 27.570253085810084 34.0 28.0 35.0 2.0 35.0 98-99 26.914434908422983 33.5 26.5 35.0 2.0 35.0 100-101 24.585539437954793 31.0 21.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.15870763860953474 1101 2 -0.05949141530803104 1101 3 -0.09294931810211438 1101 4 -0.1504167270154042 1101 5 -0.11466891980170146 1101 6 -0.12457081074290954 1101 7 -0.08831807375185718 1101 8 -0.2557753347479732 1101 9 -0.3574237234962183 1101 10-11 -0.24037099045806798 1101 12-13 -0.20500633156464687 1101 14-15 -0.463065625692785 1101 16-17 -0.2704703268040092 1101 18-19 -0.32409549159986994 1101 20-21 -0.3062254372317099 1101 22-23 -0.268335636149466 1101 24-25 -0.3907162192601774 1101 26-27 -0.4124327189855137 1101 28-29 -0.48009844366994514 1101 30-31 -0.3829012582113407 1101 32-33 -0.22174053785521863 1101 34-35 -0.2773796847510255 1101 36-37 -0.3652167365369934 1101 38-39 -0.32405566039730616 1101 40-41 -0.1626505112993044 1101 42-43 -0.23107760713313752 1101 44-45 -0.27450846574497945 1101 46-47 -0.21568787737481188 1101 48-49 -0.5189483371476129 1101 50-51 -0.5954841241596185 1101 52-53 -0.39096136479064825 1101 54-55 -0.5739367276706204 1101 56-57 -0.32015785775452343 1101 58-59 -0.4943900819790841 1101 60-61 -0.7455972479948585 1101 62-63 -0.7160498677849958 1101 64-65 -0.5528576986386398 1101 66-67 -0.6209221462393231 1101 68-69 -0.5223869352344792 1101 70-71 -0.5079413614969077 1101 72-73 -0.42215299080437774 1101 74-75 -0.5644478609731927 1101 76-77 -0.4270838366966814 1101 78-79 -0.6553908426397577 1101 80-81 -0.6472539715419714 1101 82-83 -0.6587972291361979 1101 84-85 -0.7830221044822707 1101 86-87 -0.8148301238571207 1101 88-89 -0.7034713848933407 1101 90-91 -0.7952711613843704 1101 92-93 -0.8317895033176619 1101 94-95 -0.9369109483330789 1101 96-97 -0.94942645517947 1101 98-99 -1.0373454274730385 1101 100-101 -0.6911276246114042 1102 1 -0.22109290443841445 1102 2 -0.3053973102522818 1102 3 -0.16867387103754083 1102 4 -0.07519983956298404 1102 5 -0.12226175259282002 1102 6 -0.2611673788179303 1102 7 -0.19355455904107544 1102 8 -0.26740556999289566 1102 9 -0.26859289570910505 1102 10-11 -0.24837093021155 1102 12-13 -0.35072847463948165 1102 14-15 -0.3201099494079358 1102 16-17 -0.20335438061477618 1102 18-19 -0.5516727134558863 1102 20-21 -0.30832387649258663 1102 22-23 -0.35696180298270264 1102 24-25 -0.42410962191244295 1102 26-27 -0.40866598285816025 1102 28-29 -0.2838467514516694 1102 30-31 -0.30866736352223967 1102 32-33 -0.29258689506743707 1102 34-35 -0.4159032553138715 1102 36-37 -0.5909971946940189 1102 38-39 -0.39877595709367597 1102 40-41 -0.24303752427711345 1102 42-43 -0.17574029752984188 1102 44-45 -0.24105703152354607 1102 46-47 -0.2379542803743817 1102 48-49 -0.01933905351410914 1102 50-51 -0.11471428002089823 1102 52-53 -0.3325971108196981 1102 54-55 -0.14674508350780258 1102 56-57 -0.22081224122004528 1102 58-59 -0.22471782301830245 1102 60-61 -0.27014681670068796 1102 62-63 -0.442780537513606 1102 64-65 -0.4403203103604838 1102 66-67 -0.36734678139352184 1102 68-69 -0.35031225790771714 1102 70-71 -0.2349488993990505 1102 72-73 -0.25925438923230004 1102 74-75 -0.22168506213276018 1102 76-77 -0.3258200774559299 1102 78-79 -0.20729498689117776 1102 80-81 -0.3892699793952836 1102 82-83 -0.3709407115264973 1102 84-85 -0.41231909853558335 1102 86-87 -0.27505014767221425 1102 88-89 -0.22579094658222587 1102 90-91 -0.3965526401882329 1102 92-93 -0.28375841257223655 1102 94-95 -0.19158448310240317 1102 96-97 -0.05242983252819755 1102 98-99 -0.10373221364044127 1102 100-101 0.5565914246277188 1103 1 -0.05659431016752592 1103 2 -0.01576380080386741 1103 3 -0.001999225516286174 1103 4 -0.02099984304466318 1103 5 -0.050832870299778676 1103 6 -0.05681005379420867 1103 7 -0.06380532718716836 1103 8 -0.17059848999254967 1103 9 -0.05207470827275529 1103 10-11 -0.10378810099675917 1103 12-13 -0.17924693190619223 1103 14-15 -0.1859449655542349 1103 16-17 -0.25267839029385186 1103 18-19 -0.15680046942478754 1103 20-21 -0.3353542411117374 1103 22-23 0.023468532912573892 1103 24-25 -0.13743336611327095 1103 26-27 -0.15866761489660064 1103 28-29 -0.42055553921704103 1103 30-31 -0.35402443177191145 1103 32-33 -0.4926443428204479 1103 34-35 -0.23633224205737235 1103 36-37 -0.046342990998212485 1103 38-39 -0.013666566997315499 1103 40-41 -0.1799358461063889 1103 42-43 -0.2173670699235828 1103 44-45 -0.35790717557814133 1103 46-47 -0.3449898892770378 1103 48-49 -0.36955165372126686 1103 50-51 -0.3355910981117134 1103 52-53 -0.3848217968155083 1103 54-55 -0.6009147241111776 1103 56-57 -0.5416873184698687 1103 58-59 -0.5765744299451043 1103 60-61 -0.44319838041111836 1103 62-63 -0.4436183172449901 1103 64-65 -0.6526164143583202 1103 66-67 -0.47658618619998094 1103 68-69 -0.3671810072005002 1103 70-71 -0.3702965671073244 1103 72-73 -0.3917182450210319 1103 74-75 -0.4111005312458893 1103 76-77 -0.5190751890003185 1103 78-79 -0.5912461737095285 1103 80-81 -0.6602117526159255 1103 82-83 -0.4788842778133713 1103 84-85 -0.5076377594806871 1103 86-87 -0.5348048414147577 1103 88-89 -0.36690787016800996 1103 90-91 -0.35794949100686324 1103 92-93 -0.2983559083724572 1103 94-95 -0.14267011507120841 1103 96-97 -0.1171026197016829 1103 98-99 -0.0696133620732482 1103 100-101 0.3850494964722273 1104 1 0.023469236396991278 1104 2 -0.06955161165034696 1104 3 0.002897447257339536 1104 4 -0.0804940328900301 1104 5 -0.05779224405863914 1104 6 0.01616343857635627 1104 7 -0.04181770339307889 1104 8 -0.09983759675333914 1104 9 -0.06901960468929502 1104 10-11 -0.08139370231894105 1104 12-13 -0.06298938895460537 1104 14-15 -0.18529028219806065 1104 16-17 -0.2976452717718203 1104 18-19 -0.14167292788250307 1104 20-21 -0.09596754233013627 1104 22-23 -0.22219467029550088 1104 24-25 -0.2737772606464759 1104 26-27 -0.1901084307524954 1104 28-29 -0.023778349358863693 1104 30-31 -0.1317171533841517 1104 32-33 -0.22430488117259273 1104 34-35 -0.2979064288278934 1104 36-37 -0.27058485229758134 1104 38-39 -0.2887354304618839 1104 40-41 -0.3256464530017027 1104 42-43 -0.23558033526892785 1104 44-45 -0.13366467368839352 1104 46-47 -0.2982210270936534 1104 48-49 -0.5543056029608877 1104 50-51 -0.687895942256084 1104 52-53 -0.5660294377774164 1104 54-55 -0.6508910222675581 1104 56-57 -0.7485826331919156 1104 58-59 -0.6145454240132366 1104 60-61 -0.7088915632484216 1104 62-63 -0.6066453629703332 1104 64-65 -0.5735617976235332 1104 66-67 -0.6863353310118399 1104 68-69 -0.3063076262926572 1104 70-71 -0.33165423433343477 1104 72-73 -0.3010868791542478 1104 74-75 -0.30389837373752826 1104 76-77 -0.17844894785647725 1104 78-79 -0.3821427439886982 1104 80-81 -0.4293666858023464 1104 82-83 -0.3347379925969136 1104 84-85 -0.1380824573142121 1104 86-87 -0.04139267276012859 1104 88-89 -0.08952711606502817 1104 90-91 -0.2853424163268343 1104 92-93 -0.2883396373771099 1104 94-95 -0.2590109347706431 1104 96-97 -0.2975934400417053 1104 98-99 -0.1568617702061843 1104 100-101 0.4110997443806994 1105 1 0.02122746683571819 1105 2 0.061513296659384764 1105 3 0.01960364533001524 1105 4 0.042801937617284125 1105 5 -0.009698561403212125 1105 6 0.052085966056331756 1105 7 0.03749268817114171 1105 8 0.12225616722383847 1105 9 0.048777660189202265 1105 10-11 -0.04766753975675897 1105 12-13 0.12292229562544321 1105 14-15 0.1419321734477066 1105 16-17 -0.036153830885957916 1105 18-19 -0.06579634719100369 1105 20-21 0.08325949444084557 1105 22-23 0.025670633780073615 1105 24-25 0.045327251355267606 1105 26-27 0.10607690151461924 1105 28-29 0.09161653550309268 1105 30-31 0.1362820605056001 1105 32-33 -0.07749743752179228 1105 34-35 -0.0913986558240083 1105 36-37 -0.03287799009729042 1105 38-39 -0.2034743198778557 1105 40-41 -0.4697885763125882 1105 42-43 -0.28848419940738523 1105 44-45 -0.3806537359130928 1105 46-47 -0.5426762391401141 1105 48-49 -0.8671679930071079 1105 50-51 -0.8892736682288884 1105 52-53 -0.9436795102893072 1105 54-55 -1.0354060057999064 1105 56-57 -1.1554882050871242 1105 58-59 -1.1292680490793146 1105 60-61 -0.97187864664388 1105 62-63 -0.9821248224517731 1105 64-65 -0.8453614479400144 1105 66-67 -0.7687840644255886 1105 68-69 -0.7471114917864838 1105 70-71 -0.8577049791051294 1105 72-73 -0.8738813655396314 1105 74-75 -0.8686189657598646 1105 76-77 -0.6248145433349883 1105 78-79 -0.7936585813994128 1105 80-81 -0.802153040012783 1105 82-83 -0.7912559308194922 1105 84-85 -0.9519545924167403 1105 86-87 -0.7852982853162054 1105 88-89 -0.7701467877962358 1105 90-91 -0.3682282541441637 1105 92-93 -0.29514491244792396 1105 94-95 -0.06513649555187584 1105 96-97 -0.229697233701323 1105 98-99 -0.2294211011992573 1105 100-101 0.4568350963005656 1106 1 -0.13860605793131953 1106 2 0.048085704254575035 1106 3 -0.009394956162687151 1106 4 0.03495876266930509 1106 5 -0.12397246698714781 1106 6 -0.15093147763892745 1106 7 -0.035667025206947756 1106 8 -0.06733688797795168 1106 9 -0.17925432125374385 1106 10-11 -0.27136863405267775 1106 12-13 -0.20199516360879954 1106 14-15 -0.2056865995503685 1106 16-17 -0.05004839336783817 1106 18-19 -0.039523473266513065 1106 20-21 -0.17555406515270278 1106 22-23 -0.1693610609128129 1106 24-25 -0.07150097254046983 1106 26-27 -0.0011123551298837242 1106 28-29 -0.10666552723910883 1106 30-31 -0.01832428291844934 1106 32-33 -0.0381998630428626 1106 34-35 0.1410635340173485 1106 36-37 0.14329147758886052 1106 38-39 0.19678391537573958 1106 40-41 0.38652881595989896 1106 42-43 0.2051496287700516 1106 44-45 0.5699299366343595 1106 46-47 0.4623539016557672 1106 48-49 0.5550009908884377 1106 50-51 0.6855921279350667 1106 52-53 0.5450050758534601 1106 54-55 0.3796639115552054 1106 56-57 0.44906423252872685 1106 58-59 0.4402279916872587 1106 60-61 0.5474441426172589 1106 62-63 0.661618659591106 1106 64-65 0.6639667399117144 1106 66-67 0.703505306438359 1106 68-69 0.6346081115052158 1106 70-71 0.7237816998325215 1106 72-73 0.6660938361866329 1106 74-75 0.36836314199728903 1106 76-77 0.5239310452795927 1106 78-79 0.32328498330437583 1106 80-81 0.29355660231857783 1106 82-83 0.235797095515057 1106 84-85 0.04598128770571108 1106 86-87 -0.0023160205674024326 1106 88-89 0.3609764874982453 1106 90-91 0.2804796885122336 1106 92-93 0.18250059719024136 1106 94-95 0.3378562780261092 1106 96-97 0.4878587586541485 1106 98-99 0.4664203820835304 1106 100-101 1.022866907936212 1107 1 -0.010736328794173744 1107 2 0.029769706500623272 1107 3 0.05940902255332503 1107 4 0.023231176370209994 1107 5 -0.04106650693474023 1107 6 -0.08633260244619123 1107 7 -0.0024843197870154654 1107 8 -0.004980496987109007 1107 9 -0.04043955793565601 1107 10-11 0.05368283791181483 1107 12-13 0.032995076771960896 1107 14-15 0.1440589373178156 1107 16-17 0.10537109200164707 1107 18-19 0.059597715357064374 1107 20-21 0.13959161610436865 1107 22-23 0.2090661697618046 1107 24-25 0.22954450995906228 1107 26-27 0.06824689690002828 1107 28-29 0.1514233699684837 1107 30-31 0.1265476833551631 1107 32-33 -0.1103541987202874 1107 34-35 -0.14601366056004395 1107 36-37 -0.1838304597178393 1107 38-39 0.0046700442383667 1107 40-41 -0.3241194188413843 1107 42-43 -0.4264332121258789 1107 44-45 -0.21258029091183062 1107 46-47 -0.35411801827078904 1107 48-49 -0.6283648745090389 1107 50-51 -0.6867761172051772 1107 52-53 -0.8721451153281308 1107 54-55 -0.48870868307745496 1107 56-57 -0.46945587050433346 1107 58-59 -0.49620970272525255 1107 60-61 -0.5972370877186748 1107 62-63 -0.6471890625404981 1107 64-65 -0.7155835154427095 1107 66-67 -0.8255970759782798 1107 68-69 -0.5552971444285433 1107 70-71 -0.4383090869086601 1107 72-73 -0.2767335121381862 1107 74-75 -0.30073496223009855 1107 76-77 -0.2567386695969205 1107 78-79 -0.3537625313350503 1107 80-81 -0.35295088005617714 1107 82-83 -0.3725141784049235 1107 84-85 -0.41325024254911114 1107 86-87 -0.3654255511252593 1107 88-89 -0.06259098756793335 1107 90-91 -0.06490655176157034 1107 92-93 0.031771968082512814 1107 94-95 0.13401749445792177 1107 96-97 0.21955835846519278 1107 98-99 0.5120018147421597 1107 100-101 1.110926303463721 1108 1 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100-101 0.15566903277021282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 3.0 5 15.0 6 26.0 7 65.0 8 133.0 9 295.0 10 584.0 11 1006.0 12 1787.0 13 2713.0 14 3498.0 15 5441.0 16 8298.0 17 13282.0 18 12105.0 19 5756.0 20 3995.0 21 3710.0 22 3774.0 23 3946.0 24 4308.0 25 4812.0 26 5785.0 27 6891.0 28 8664.0 29 10683.0 30 13527.0 31 17419.0 32 22608.0 33 31328.0 34 45111.0 35 67402.0 36 107526.0 37 176431.0 38 167491.0 39 19194.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.15174657776009 19.96880996540858 15.247306169337502 19.632137287493823 2 15.287891362040593 22.34507456866641 40.35827848802681 22.008755581266186 3 20.112055021888953 27.759656344076646 30.59309891908463 21.535189714949777 4 11.986270181383937 17.959716117016434 39.03981057365347 31.01420312794616 5 12.15232348730894 40.025192277104786 33.489181687112946 14.333302548473329 6 28.622387659581566 38.123463187693446 18.192266202507128 15.061882950217864 7 26.587285808287913 35.720616661771096 20.64757438588435 17.044523144056633 8 26.4201524594769 34.46012593363152 19.67312154557943 19.446600061312157 9 27.751318916852764 16.98436539993356 20.981969351617526 34.28234633159615 10-11 24.14598762977576 28.360765712262737 26.722070152665296 20.77117650529621 12-13 26.87218877453178 25.93443483023774 27.717456487537127 19.475919907693353 14-15 23.615698477585788 26.321706684179542 28.220844337668368 21.841750500566302 16-17 20.116480271723166 30.05033240681001 28.87494757040957 20.95823975105725 18-19 19.984107581030173 27.963346104561598 32.835610306086195 19.216936008322037 20-21 21.65499213075138 26.57503620367437 32.57118559497805 19.198786070596203 22-23 20.95052593570764 26.740789150479632 32.13931828778602 20.169366626026715 24-25 20.648989497339702 26.75882361995454 32.8680163979005 19.724170484805263 26-27 20.93621791767865 26.522882113041625 33.084129291939426 19.456770677340305 28-29 20.170755547118116 26.88707198875312 33.500129554614325 19.44204290951444 30-31 20.26851698048589 27.217134600325416 33.06431183063959 19.4500365885491 32-33 20.071778834992056 27.40089043722751 32.58715497481456 19.94017575296588 34-35 20.61454534726667 26.722095593092504 32.84311399058769 19.82024506905314 36-37 20.95323729472292 26.68823281175244 32.77166865268459 19.586861240840054 38-39 20.928356258125007 26.780992400731385 32.96109808116704 19.329553259976564 40-41 20.582542453538082 27.09471445594439 32.98266969550433 19.340073395013192 42-43 20.16528056260046 27.68705990714835 32.23759914717077 19.91006038308042 44-45 20.681990032265908 27.588920435778917 31.5638285796011 20.16526095235408 46-47 20.72350910767723 27.892477973158613 30.674805705878654 20.709207213285495 48-49 21.128900472924524 27.982522187176524 30.394924687378545 20.493652652520407 50-51 21.400957136645303 28.417286949874104 30.224135697400133 19.95762021608046 52-53 21.0591226204662 28.778489950834835 29.837535610434358 20.32485181826461 54-55 20.377698280052897 28.634252459861703 30.172764318889918 20.815284941195483 56-57 21.237158758723833 29.043107064109684 29.236150571370302 20.48358360579618 58-59 21.19893486160512 28.367069984364118 29.47319004075078 20.96080511327998 60-61 21.05052865702005 28.41472158765137 30.03455542914021 20.500194326188375 62-63 21.57347774612406 28.144588945597647 30.008709404746174 20.27322390353212 64-65 21.194124807437497 28.295432244294318 30.124984447491528 20.38545850077666 66-67 20.879932069208344 28.477572962108315 29.882493583387742 20.760001385295602 68-69 20.960676845168848 28.80523385200682 29.655266624509213 20.57882267831512 70-71 20.983829239229006 29.04259399166514 29.131740328905092 20.841836440200765 72-73 21.068229656356905 28.429793090709925 29.125006253070417 21.376970999862756 74-75 20.906034629824642 29.01629902888213 28.96159267948236 21.116073661810862 76-77 21.080094456637042 28.455639115103953 29.36987007723023 21.094396351028774 78-79 20.935600429441635 28.291648335015786 29.501473159256403 21.271278076286176 80-81 20.878804437056193 29.20149150350942 28.99196527521241 20.927738784221976 82-83 20.767547488305095 29.17357714660992 28.87599360195379 21.182881763131196 84-85 20.777656563557617 28.527606378305094 28.69345821604735 22.00127884208994 86-87 20.889359775376885 28.629955478138626 28.774000566945052 21.70668417953944 88-89 21.178347829767695 27.881575183712005 29.135203567905783 21.804873418614527 90-91 20.716936500789554 28.02740801459298 29.306281933364374 21.94937355125309 92-93 20.613025787848002 28.30070087943437 29.223747806544964 21.862525526172664 94-95 20.281497196700432 28.307938385130132 29.072031523170992 22.33853289499844 96-97 20.03131024592845 28.239443213783176 29.063437559724843 22.665808980563533 98-99 20.259396933875138 28.2446101670566 28.69066309567787 22.805329803390386 100-101 20.308545288244567 28.829203275311116 28.476618573523243 22.38563286292107 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 473.0 1 333.5 2 246.5 3 318.5 4 297.5 5 297.5 6 493.5 7 1000.0 8 1554.5 9 1920.5 10 1936.5 11 1786.0 12 1792.5 13 1870.0 14 2019.5 15 2179.0 16 2277.5 17 2355.0 18 2464.5 19 2574.5 20 2741.0 21 3002.0 22 3318.0 23 3737.0 24 4256.5 25 4861.5 26 5826.0 27 7098.5 28 8315.0 29 9930.5 30 11770.5 31 13120.0 32 14779.0 33 16715.0 34 18896.0 35 21167.5 36 23581.0 37 25321.0 38 26015.5 39 26369.0 40 26394.0 41 26552.5 42 26455.5 43 27082.0 44 28379.0 45 28663.0 46 28387.5 47 30309.5 48 40658.0 49 39521.5 50 28554.0 51 24525.5 52 20463.0 53 18479.0 54 16891.5 55 15036.0 56 13231.5 57 11468.5 58 10347.5 59 9226.0 60 7810.5 61 6253.0 62 4723.5 63 3351.5 64 2242.0 65 1565.0 66 1079.0 67 787.0 68 568.0 69 398.5 70 312.5 71 237.0 72 186.0 73 168.5 74 141.0 75 115.0 76 92.0 77 63.0 78 42.5 79 29.0 80 17.5 81 13.5 82 10.5 83 4.5 84 4.0 85 2.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06695595401331679 2 0.0 3 0.0 4 0.0 5 0.0016674854447760886 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.8480433340986665E-4 12-13 0.005323126612169822 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.337427223880443E-4 24-25 6.413405556831111E-4 26-27 1.9240216670493332E-4 28-29 0.002950166556142311 30-31 1.9240216670493332E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.2067027784155554E-4 40-41 0.0 42-43 0.012057202446842488 44-45 1.9240216670493332E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2826811113662222E-4 82-83 5.130724445464889E-4 84-85 7.054746112514222E-4 86-87 0.0 88-89 0.0 90-91 0.008209159112743822 92-93 0.0032067027784155556 94-95 0.0 96-97 0.0 98-99 1.2826811113662222E-4 100-101 0.011287593780022755 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 779617.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.3864097453642 #Duplication Level Percentage of deduplicated Percentage of total 1 80.99373427040257 48.099270901892744 2 11.506462138626743 13.666549505680148 3 3.346207163068407 5.961576890365595 4 1.3723075640417142 3.2598567717937543 5 0.7139550404001738 2.1199613284486385 6 0.4213806320922855 1.5014569725715818 7 0.2895774744385175 1.203787658502347 8 0.21047287198476655 0.9999382572776747 9 0.15544948812285697 0.8308428296734 >10 0.8829728140243094 9.680850731726041 >50 0.06444502588703532 2.594027721071374 >100 0.03691879369780019 4.1344362913764785 >500 0.003495270407380671 1.4764824568758967 >1k 0.0021845440046129195 2.6994681453891274 >5k 4.3690880092258385E-4 1.7714935373552738 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7454 0.956110500412382 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6201 0.7953905571581944 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4051 0.5196141182144566 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3577 0.45881503353569764 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3142 0.403018405191267 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2300 0.29501665561423107 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1941 0.24896840371618373 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1569 0.20125266637336026 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1141 0.14635391480688595 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1042 0.13365537180436035 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1040 0.1333988355820871 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1005 0.1289094516923053 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 961 0.12326565480229394 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 902 0.11569783624523323 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 891 0.11428688702273039 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 865 0.11095191613317822 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 850 0.10902789446612887 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 843 0.10813001768817253 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 837 0.1073604090213528 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.413405556831111E-5 0.0 22-23 0.0 0.0 0.0 2.5653622227324443E-4 0.0 24-25 0.0 0.0 0.0 6.413405556831111E-4 0.0 26-27 0.0 0.0 0.0 0.0011544130002296 0.0 28-29 0.0 0.0 0.0 0.003591507111825422 0.0 30-31 0.0 0.0 0.0 0.0080808910016072 0.0 32-33 0.0 0.0 0.0 0.013532285724913645 0.0 34-35 0.0 0.0 0.0 0.019240216670493332 0.0 36-37 0.0 0.0 0.0 0.026936303338690664 0.0 38-39 0.0 0.0 0.0 0.03431171972904644 0.0 40-41 0.0 0.0 0.0 0.04418836428656635 0.0 42-43 0.0 0.0 0.0 0.06060668251205399 0.0 44-45 0.0 0.0 0.0 0.08504175768358052 0.0 46-47 0.0 0.0 0.0 0.13634900213822942 0.0 48-49 0.0 0.0 0.0 0.23748840776945604 0.0 50-51 0.0 0.0 0.0 0.3120121803398336 0.0 52-53 0.0 0.0 0.0 0.387562097799304 0.0 54-55 0.0 0.0 0.0 0.4843403876518855 0.0 56-57 0.0 0.0 0.0 0.5670733193350068 0.0 58-59 0.0 0.0 0.0 0.6819374128578519 0.0 60-61 0.0 0.0 0.0 0.8697219275618668 0.0 62-63 0.0 0.0 0.0 1.001068473365768 0.0 64-65 0.0 0.0 0.0 1.1536433915627802 0.0 66-67 0.0 0.0 0.0 1.402034588778849 0.0 68-69 0.0 0.0 0.0 1.6783241001671332 0.0 70-71 0.0 0.0 0.0 1.9655164010020303 0.0 72-73 0.0 0.0 0.0 2.2898423200109796 0.0 74-75 0.0 0.0 0.0 2.577419425179287 0.0 76-77 0.0 0.0 0.0 2.80817375711407 0.0 78-79 0.0 0.0 0.0 3.1036393511172795 0.0 80-81 0.0 0.0 0.0 3.458621348687881 0.0 82-83 0.0 0.0 0.0 3.862986569046083 0.0 84-85 0.0 0.0 0.0 4.274470669572366 0.0 86-87 0.0 0.0 0.0 4.963013890153755 0.0 88-89 0.0 0.0 0.0 5.909824952508732 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTGCG 50 0.001615717 37.998947 5 GGTCGGG 175 1.5097612E-10 29.88123 1 GTCGGGA 150 3.3345714E-8 28.499212 2 TACCTGG 730 0.0 27.32801 2 GTGAGCG 90 8.9430506E-4 26.38985 9 GTACCCG 115 1.2742399E-4 24.8026 1 GTGCTAG 310 0.0 23.002415 1 GAGTACT 5715 0.0 22.649557 12-13 GGACGTG 105 0.0021959457 22.618422 6 TTGCGCG 120 5.9335434E-9 21.77023 18-19 AGTACTT 5830 0.0 21.754652 12-13 CGGCAAT 55 0.002877213 21.591696 90-91 TACCCGG 110 0.0028770072 21.590311 2 CGGTATA 110 0.0028770072 21.590311 2 GTACTTT 6095 0.0 21.236082 14-15 AGCGACT 135 3.7935007E-4 21.11188 8 GTACCTG 930 0.0 20.957752 1 GTGTAGC 480 0.0 20.798016 1 CGGGAGT 210 8.602674E-7 20.356579 4 GCGGTAT 145 6.110325E-4 19.67103 1 >>END_MODULE