FastQCFastQC Report
Thu 26 May 2016
SRR937299_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937299_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430741
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.5309455101789706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18460.42856380052049836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12130.2816077410787457No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6800.15786748881578488No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13300.044.32951
GTATCAA24150.040.951421
ATCAACG30350.032.562993
TATCAAC30700.032.5050662
TCAACGC30850.032.0314984
GTACACG600.0039291731.6979731
CAACGCA31200.031.6758545
GTATAGG1401.6843842E-830.5659051
AACGCAG32100.030.4810756
CGCAGAG35450.027.0678488
ACGCAGA36250.026.7325737
TACATGG21300.025.6559642
GCAGAGT36550.025.6033889
CATGGGG7500.025.3377384
GTACATG22550.024.880451
GAGTACT20500.024.2119912-13
TACCTGG3150.024.1367932
ACATGGG21900.024.0823273
TATATGG2354.6075E-922.2430822
AGTACTT21250.022.2398712-13
Produced by FastQC (version 0.11.5)