##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937299_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 430741 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96715891916488 33.0 31.0 34.0 31.0 34.0 2 32.100707849960884 33.0 31.0 34.0 30.0 34.0 3 32.12196192143306 34.0 31.0 34.0 30.0 34.0 4 35.72507841138874 37.0 35.0 37.0 35.0 37.0 5 35.729073851804216 37.0 35.0 37.0 35.0 37.0 6 35.77736969547826 37.0 35.0 37.0 35.0 37.0 7 35.73109362702877 37.0 35.0 37.0 35.0 37.0 8 35.71877764132042 37.0 35.0 37.0 35.0 37.0 9 37.36017931889465 39.0 37.0 39.0 35.0 39.0 10-11 37.25526476467297 39.0 37.0 39.0 34.5 39.0 12-13 37.136664492119394 39.0 37.0 39.0 34.0 39.0 14-15 38.29857501375537 40.0 38.0 41.0 34.0 41.0 16-17 38.227341952588674 40.0 38.0 41.0 34.0 41.0 18-19 38.171881710819264 40.0 38.0 41.0 33.5 41.0 20-21 38.06321199978642 40.0 38.0 41.0 34.0 41.0 22-23 38.126694463726466 40.0 38.0 41.0 34.0 41.0 24-25 38.10616472543826 40.0 38.0 41.0 34.0 41.0 26-27 38.019915680188326 40.0 38.0 41.0 34.0 41.0 28-29 37.8726705839472 40.0 38.0 41.0 33.0 41.0 30-31 37.6187465321388 40.0 37.0 41.0 33.0 41.0 32-33 37.37576409025377 40.0 37.0 41.0 32.5 41.0 34-35 37.39134421845146 40.0 37.0 41.0 33.0 41.0 36-37 37.279900218460746 40.0 37.0 41.0 32.5 41.0 38-39 37.13853220380693 40.0 37.0 41.0 32.0 41.0 40-41 36.91427214962124 39.5 36.0 41.0 31.0 41.0 42-43 36.64071100731066 39.0 36.0 41.0 30.5 41.0 44-45 36.36334943736492 39.0 36.0 41.0 30.0 41.0 46-47 35.91375908028258 39.0 35.5 41.0 29.0 41.0 48-49 35.541566741963265 39.0 35.0 40.0 27.5 41.0 50-51 34.59917560668708 38.0 34.0 39.5 25.0 40.5 52-53 34.695009297930774 38.0 34.5 39.5 26.0 40.5 54-55 35.0015322432738 39.0 35.0 40.0 26.0 41.0 56-57 34.757610721988385 38.0 34.5 40.0 24.5 41.0 58-59 34.49845034487082 38.0 34.0 40.0 23.5 41.0 60-61 34.17556257704746 38.0 34.0 40.0 22.5 41.0 62-63 33.80036843485993 38.0 33.5 40.0 22.0 41.0 64-65 33.38684267343949 37.0 33.0 40.0 20.0 41.0 66-67 32.953285152794834 36.5 32.5 39.0 18.5 41.0 68-69 32.44349388611718 36.0 32.0 39.0 14.5 40.0 70-71 31.99263130280145 35.5 31.0 39.0 10.0 40.0 72-73 31.502030222337787 35.0 31.0 38.0 7.5 40.0 74-75 30.959452431971883 35.0 30.5 37.5 4.5 39.5 76-77 30.420576402060632 34.5 30.0 37.0 2.0 39.0 78-79 29.87192651732712 34.0 29.0 36.0 2.0 39.0 80-81 29.286907909857664 34.0 29.0 36.0 2.0 37.5 82-83 28.72076143204385 34.0 28.5 35.0 2.0 37.0 84-85 28.12322486134359 34.0 27.0 35.0 2.0 36.5 86-87 27.505426694928044 33.0 26.0 35.0 2.0 36.0 88-89 26.937422952539926 33.0 25.5 35.0 2.0 36.0 90-91 26.371327549501906 32.0 24.5 35.0 2.0 35.0 92-93 25.69594605575044 32.0 21.5 34.5 2.0 35.0 94-95 24.901822673021606 31.5 19.0 34.0 2.0 35.0 96-97 24.042194729547454 31.0 6.0 34.0 2.0 35.0 98-99 22.821194174689662 31.0 2.0 34.0 2.0 35.0 100-101 19.72298550637158 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11605147538484673 1101 2 0.252122832533864 1101 3 0.18126328276164116 1101 4 0.15910290764770707 1101 5 0.12299923607270102 1101 6 0.07775091676924717 1101 7 0.14477117982968224 1101 8 0.14261554664862786 1101 9 0.1652363083953574 1101 10-11 0.20963529424636107 1101 12-13 0.4139731985328723 1101 14-15 0.5785117514812583 1101 16-17 0.5653724319969413 1101 18-19 0.4417950850265058 1101 20-21 0.4848104383719587 1101 22-23 0.5504083543811831 1101 24-25 0.449353156812343 1101 26-27 0.36232473729649683 1101 28-29 0.38276652922827026 1101 30-31 0.4976134905261276 1101 32-33 0.3673299943000998 1101 34-35 0.6237155133854984 1101 36-37 0.6939583256389668 1101 38-39 0.6018616062434674 1101 40-41 0.5980917382444346 1101 42-43 0.6357263572052858 1101 44-45 0.9855263712956344 1101 46-47 1.4159687324725994 1101 48-49 1.4516063221449187 1101 50-51 1.141131513166357 1101 52-53 1.1941101593281473 1101 54-55 1.4656110715035098 1101 56-57 1.356857205042708 1101 58-59 1.3227374724245493 1101 60-61 1.0825893573514023 1101 62-63 1.276952889274213 1101 64-65 1.2310851603607986 1101 66-67 1.2557255757887162 1101 68-69 1.219141817706813 1101 70-71 1.147116785833127 1101 72-73 1.257332572230581 1101 74-75 1.392221711614809 1101 76-77 1.2828105360755089 1101 78-79 1.3270836658964313 1101 80-81 1.3462101192957583 1101 82-83 1.2354990782989894 1101 84-85 1.365028579423253 1101 86-87 1.4969390288284004 1101 88-89 1.4818566017959718 1101 90-91 1.9301896741928815 1101 92-93 2.021595141236073 1101 94-95 1.8086090217218036 1101 96-97 1.6924118809705213 1101 98-99 2.0894241678716554 1101 100-101 1.8958966305522544 1102 1 0.11318542144609367 1102 2 -0.10094994271567259 1102 3 -0.205299194664736 1102 4 0.04258899804419514 1102 5 -0.1532679676869506 1102 6 -0.14424185117173494 1102 7 -0.18192072680791682 1102 8 -0.17157322623837246 1102 9 -0.17128176537844553 1102 10-11 -0.2056387124078114 1102 12-13 -0.01341514433980251 1102 14-15 -0.07789852127189789 1102 16-17 -0.008005135022834509 1102 18-19 0.08034829080025219 1102 20-21 -0.003285558500664365 1102 22-23 -0.13568437914165798 1102 24-25 -0.0027248916364968068 1102 26-27 -0.0024693274860965175 1102 28-29 0.055328896176661146 1102 30-31 0.12168983081532048 1102 32-33 0.25648020612039346 1102 34-35 0.33796632391150183 1102 36-37 0.26197346112322606 1102 38-39 0.20617194740798794 1102 40-41 0.26757771816293285 1102 42-43 0.5470507758148742 1102 44-45 0.6996943233311939 1102 46-47 0.8151108284099919 1102 48-49 0.9347350587441454 1102 50-51 0.9091213661881383 1102 52-53 0.8053583056349964 1102 54-55 0.8929569228426288 1102 56-57 1.0428171525231775 1102 58-59 1.022803546759235 1102 60-61 1.0882619770819204 1102 62-63 1.114466327221706 1102 64-65 0.9521282425266762 1102 66-67 1.1473020541753982 1102 68-69 1.0095640135842814 1102 70-71 1.127431520622224 1102 72-73 0.9054963006077017 1102 74-75 1.0743712308231075 1102 76-77 0.9499403604579975 1102 78-79 1.277187982611327 1102 80-81 1.3749428942903386 1102 82-83 1.2620297295667235 1102 84-85 1.0408946631060765 1102 86-87 1.1915321936403096 1102 88-89 1.003596332654599 1102 90-91 1.0681479984967623 1102 92-93 1.2296974268556653 1102 94-95 1.0133236698281678 1102 96-97 1.1117321439081316 1102 98-99 1.7474321222867388 1102 100-101 1.8060737777556746 1103 1 0.3341494629698438 1103 2 0.186547501801968 1103 3 0.33452089937701857 1103 4 0.2743144597346756 1103 5 0.2004846161896623 1103 6 0.17224612102461379 1103 7 0.16505131053619948 1103 8 0.27324539130245995 1103 9 0.23824848017501665 1103 10-11 0.30651118650866493 1103 12-13 0.20392011223061957 1103 14-15 0.046238293574887734 1103 16-17 0.2303219894348345 1103 18-19 0.08078840165139667 1103 20-21 0.1832760115877008 1103 22-23 0.08684702489713203 1103 24-25 0.07997903585594912 1103 26-27 -0.12518359048497985 1103 28-29 -0.23577745732037414 1103 30-31 -0.24796353219946354 1103 32-33 -0.22502378043208893 1103 34-35 -0.317043557286965 1103 36-37 -0.02891425666066283 1103 38-39 -0.03335909318442987 1103 40-41 -0.17585526005299812 1103 42-43 -0.05507765704976464 1103 44-45 -0.012115412488718391 1103 46-47 0.01152804213917591 1103 48-49 0.19242990007708727 1103 50-51 0.23495388555301844 1103 52-53 0.09297784542101795 1103 54-55 0.20765605738593962 1103 56-57 0.18566577710577548 1103 58-59 0.5690520986525627 1103 60-61 0.7054677558793614 1103 62-63 0.845836860806422 1103 64-65 0.6733194380381136 1103 66-67 1.0003561395398748 1103 68-69 1.0752853410721883 1103 70-71 0.9511997781239785 1103 72-73 0.9856049937908118 1103 74-75 1.0815862093570523 1103 76-77 1.0357279033333135 1103 78-79 0.9682897930286103 1103 80-81 0.6187387510016045 1103 82-83 0.9092879809353285 1103 84-85 1.100749738861932 1103 86-87 0.8735847570730648 1103 88-89 1.1137660166833996 1103 90-91 1.224776351857507 1103 92-93 1.4286729623711878 1103 94-95 1.375310585758477 1103 96-97 1.7379701331410295 1103 98-99 1.9471856602100992 1103 100-101 1.5810919500746863 1104 1 0.1687359291352557 1104 2 0.36323923112527723 1104 3 0.47737804223415736 1104 4 0.28935205371963946 1104 5 0.46552221017461903 1104 6 0.3846521360622077 1104 7 0.2703144684309322 1104 8 0.2882829852874238 1104 9 0.32283494634042853 1104 10-11 0.3553833669597921 1104 12-13 0.3928298866667177 1104 14-15 0.5218022033493313 1104 16-17 0.5235550721416118 1104 18-19 0.4012771234559054 1104 20-21 0.45489255294108233 1104 22-23 0.42519288955878665 1104 24-25 0.4681369305927916 1104 26-27 0.41522994334960117 1104 28-29 0.6082075050856375 1104 30-31 0.3873748136651969 1104 32-33 0.40091606919197176 1104 34-35 0.5560767434649136 1104 36-37 0.5998451418355799 1104 38-39 0.5258514331313577 1104 40-41 0.6512124091199354 1104 42-43 0.8067644482133929 1104 44-45 0.7181477454060143 1104 46-47 0.9137836812369216 1104 48-49 1.1369787722575424 1104 50-51 0.8721719306658002 1104 52-53 0.8937297251202594 1104 54-55 1.1390470348295452 1104 56-57 1.2796507395117942 1104 58-59 1.4337137527878951 1104 60-61 1.0945655002402717 1104 62-63 1.2950849811071734 1104 64-65 1.604710415481719 1104 66-67 1.2766719290135526 1104 68-69 1.2980297019744427 1104 70-71 1.3553351164698455 1104 72-73 1.399138828377275 1104 74-75 1.1746313221390103 1104 76-77 1.303585046190456 1104 78-79 1.495545432126356 1104 80-81 1.488099653257244 1104 82-83 1.6028970034917194 1104 84-85 1.7417271824709566 1104 86-87 1.5972689675993799 1104 88-89 1.4351945881119725 1104 90-91 1.7886861262935945 1104 92-93 1.8375075488373547 1104 94-95 1.7822654729765226 1104 96-97 1.9785716369004298 1104 98-99 2.0439901714882964 1104 100-101 1.9654904463152896 1105 1 -0.20290298024675835 1105 2 -0.22899553114574545 1105 3 -0.3103140481169788 1105 4 -0.11961006975187871 1105 5 -0.3291000448366006 1105 6 -0.32187147986697084 1105 7 -0.2501267703654264 1105 8 -0.27975113969099397 1105 9 -0.15634316595492237 1105 10-11 -0.22700750060578656 1105 12-13 -0.02271510838877333 1105 14-15 -0.16711541951786302 1105 16-17 -0.2123157897730934 1105 18-19 -0.0814013891094234 1105 20-21 -0.2337032842510638 1105 22-23 -0.21655370029790078 1105 24-25 -0.37591866769310656 1105 26-27 -0.10861947665134153 1105 28-29 -0.07075830714849474 1105 30-31 -0.035953862932352365 1105 32-33 -0.030661308490230965 1105 34-35 -0.23202150319557546 1105 36-37 -0.26963348688654065 1105 38-39 -0.3889185293531412 1105 40-41 -0.3136148469115838 1105 42-43 -0.2027615850325617 1105 44-45 -0.13316176532278945 1105 46-47 0.13235660101025104 1105 48-49 0.1270289237322615 1105 50-51 0.2890354621458542 1105 52-53 0.080073062587104 1105 54-55 0.1575281279590257 1105 56-57 0.10382037550156298 1105 58-59 -0.008141145417951634 1105 60-61 0.07531329875533999 1105 62-63 0.11150036717150158 1105 64-65 0.2692037207699869 1105 66-67 0.04575931344540152 1105 68-69 0.03777768885186461 1105 70-71 0.4005852459689585 1105 72-73 0.4290158434619613 1105 74-75 0.8216559819095188 1105 76-77 0.4706153568313596 1105 78-79 0.5132632653314957 1105 80-81 1.0164795801098663 1105 82-83 1.006223953434425 1105 84-85 0.8487837068717283 1105 86-87 0.865410616083846 1105 88-89 1.1971595213218222 1105 90-91 0.9739326455144024 1105 92-93 1.1358715433748472 1105 94-95 0.9976676265860185 1105 96-97 0.398853160123938 1105 98-99 0.865381462869145 1105 100-101 0.9245765749981665 1106 1 0.22175308741557487 1106 2 -0.30824717720323846 1106 3 -0.016332194897131558 1106 4 0.19686121122879285 1106 5 0.26509325316671806 1106 6 -0.1368439117496294 1106 7 0.18854755113770238 1106 8 0.15424596967145732 1106 9 0.06946112717714215 1106 10-11 -0.3890136833583071 1106 12-13 -0.48006147635622654 1106 14-15 0.043262103098697935 1106 16-17 0.03591770950423978 1106 18-19 0.014444657676072836 1106 20-21 0.10920863159926597 1106 22-23 -0.008210330028639135 1106 24-25 0.09760724098415352 1106 26-27 0.3663818616067829 1106 28-29 0.45784361459016765 1106 30-31 0.15942513176815254 1106 32-33 0.5263016485249139 1106 34-35 0.696993940303166 1106 36-37 0.45654217287471255 1106 38-39 0.5396117955759294 1106 40-41 0.7553188290158275 1106 42-43 0.9246071318455478 1106 44-45 0.8783439088916509 1106 46-47 0.9936829486361844 1106 48-49 1.5170274516483246 1106 50-51 1.3837272613000735 1106 52-53 1.3030101376901513 1106 54-55 1.500624054301305 1106 56-57 1.4527879093594933 1106 58-59 1.2729633190111542 1106 60-61 1.3573378156441578 1106 62-63 1.4224346051673251 1106 64-65 1.4636124783311502 1106 66-67 1.3018550791966987 1106 68-69 1.3870864785312165 1106 70-71 1.3463824463329672 1106 72-73 1.7013848279917028 1106 74-75 1.4012555750790519 1106 76-77 1.5623328773067087 1106 78-79 1.6909739071600924 1106 80-81 1.655139849902703 1106 82-83 1.423506218837776 1106 84-85 1.0155005582180152 1106 86-87 1.254850421233467 1106 88-89 1.9694147539110851 1106 90-91 1.6415996601281826 1106 92-93 1.9673513892075114 1106 94-95 2.5117695215595184 1106 96-97 2.097691552072977 1106 98-99 2.3033766183747417 1106 100-101 1.968755821291193 1107 1 0.2686142722077882 1107 2 0.09891462651029315 1107 3 0.17498080245034942 1107 4 0.19774538452676182 1107 5 -0.10305440377404551 1107 6 0.03778031459873432 1107 7 0.1252186386708587 1107 8 0.06008151343803547 1107 9 -0.0965424095160543 1107 10-11 0.1280619134586587 1107 12-13 0.2666781346075169 1107 14-15 0.2252360199864114 1107 16-17 0.3257055079127298 1107 18-19 0.3782819697892492 1107 20-21 0.16905611170537327 1107 22-23 0.09065129685022555 1107 24-25 -0.07419868297694165 1107 26-27 0.14401227716822973 1107 28-29 0.35312433961227185 1107 30-31 0.13190523786894204 1107 32-33 0.2744227802667396 1107 34-35 0.5587866016647922 1107 36-37 0.6819511030250212 1107 38-39 0.7791122376625808 1107 40-41 0.5106238688036271 1107 42-43 0.849341879856155 1107 44-45 1.3007024411639776 1107 46-47 1.2520273341357395 1107 48-49 0.8767202014272826 1107 50-51 0.9506481277899113 1107 52-53 0.9678531969297595 1107 54-55 0.6738114990404682 1107 56-57 1.011419651180887 1107 58-59 0.5983943534939087 1107 60-61 0.7945615114885456 1107 62-63 0.48990807996838726 1107 64-65 0.5376619920358365 1107 66-67 0.7635040623974163 1107 68-69 0.5428667614666693 1107 70-71 0.660429749604404 1107 72-73 0.809364492005848 1107 74-75 0.5188506237085839 1107 76-77 0.6435662990573725 1107 78-79 0.305087523428881 1107 80-81 -0.01810285566758907 1107 82-83 0.25263972900576803 1107 84-85 -0.2464910421356521 1107 86-87 -0.22602933119800994 1107 88-89 -0.2065357323842285 1107 90-91 -0.14802235578693868 1107 92-93 -0.07210484421423757 1107 94-95 0.3885931289865745 1107 96-97 0.5640929667502519 1107 98-99 0.8762656546258469 1107 100-101 0.8932142651461881 1108 1 0.6407177705312002 1108 2 0.3194741580652689 1108 3 0.38406693427558025 1108 4 0.2448776601886422 1108 5 0.16597617527605735 1108 6 0.3502146271957045 1108 7 0.4880758530244975 1108 8 0.36542158650745193 1108 9 0.6393692065191772 1108 10-11 0.550481962505259 1108 12-13 0.33945352122481154 1108 14-15 0.6065482666207132 1108 16-17 0.4595388996348646 1108 18-19 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-0.1595178635491905 2316 66-67 0.5534576432992679 2316 68-69 0.4770971622918978 2316 70-71 0.9446730446235634 2316 72-73 0.29486773397460553 2316 74-75 0.621608139181614 2316 76-77 0.5877068089306405 2316 78-79 0.07558929177547924 2316 80-81 0.3052466875095412 2316 82-83 0.11260878294034171 2316 84-85 -0.034980211012754836 2316 86-87 -0.15139957876653298 2316 88-89 -0.1344848187384855 2316 90-91 0.23882188235040402 2316 92-93 0.5026077994639238 2316 94-95 1.2502844788244758 2316 96-97 1.0007364238678456 2316 98-99 1.1962719175200398 2316 100-101 1.4941297529151214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1769.0 3 286.0 4 289.0 5 282.0 6 318.0 7 393.0 8 492.0 9 570.0 10 752.0 11 972.0 12 1403.0 13 1899.0 14 2148.0 15 2895.0 16 3863.0 17 5363.0 18 4675.0 19 2872.0 20 2919.0 21 3138.0 22 3366.0 23 3645.0 24 4097.0 25 4720.0 26 5414.0 27 6279.0 28 7464.0 29 8951.0 30 11076.0 31 13669.0 32 17537.0 33 23299.0 34 31604.0 35 43479.0 36 62555.0 37 81844.0 38 58394.0 39 6050.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11448767858141 19.568051856129046 15.28815169407864 22.029308771210907 2 14.37967824824042 20.29475291401333 41.99973001154434 23.325838826201913 3 18.35321062480798 25.720377249578714 31.0237037864611 24.902708339152213 4 11.78225795189528 16.95488844304381 39.044236417888214 32.2186171871727 5 12.594642528797165 37.97524921853725 35.16850035960088 14.261607893064706 6 29.919376417893083 37.27159560235007 18.54714676284102 14.261881216915828 7 26.936321501033078 33.69227332799732 21.826778102488692 17.54462706848091 8 25.652200215926435 34.85397044041547 20.801301142920963 18.692528200737126 9 26.067975285945337 16.52932757758049 21.362298267456335 36.040398869017835 10-11 23.656096655616075 27.40705228530768 28.39636901444179 20.54048204463445 12-13 24.832217888691346 24.87433794714747 29.062607627221194 21.23083653693999 14-15 21.94531152744022 25.832002168644703 27.80937799221196 24.41330831170312 16-17 20.577823123395277 29.39873495034151 28.343092077326947 21.680349848936274 18-19 20.517545022880768 27.456570746585335 31.465066990510355 20.560817240023542 20-21 21.306873702921276 26.555563827906152 31.587414962820954 20.550147506351614 22-23 20.922765877996774 26.658702027493124 31.4913457254433 20.9271863690668 24-25 20.718584886125957 26.71160051836014 31.61275612644451 20.957058469069395 26-27 20.586832560552587 26.963029827863377 31.522242359241847 20.92789525234219 28-29 20.422496704697885 26.963889538448292 31.69283712423958 20.920776632614242 30-31 20.69438158058552 26.820456552573123 31.55853630441182 20.926625562429532 32-33 20.35559257867445 27.082114149359587 31.390433996530632 21.171859275435327 34-35 20.601854006310322 27.027005016613458 31.335684967843413 21.035456009232803 36-37 20.69307690875926 26.903989688419017 31.210214793582253 21.192718609239474 38-39 20.610956077280907 27.08449414832918 31.31146987968625 20.99307989470366 40-41 20.78877852854861 27.013442920802273 31.160892813731156 21.036885736917956 42-43 20.547322134847146 27.204527476618118 30.948420268949796 21.299730119584943 44-45 20.6857682612322 27.301956961504054 30.764765619456313 21.247509157807432 46-47 20.828054598694017 27.420219851299642 30.214488221587025 21.537237328419312 48-49 20.900443497158918 27.63887790099729 29.90243527873308 21.558243323110716 50-51 21.006604269737178 27.699652333369013 29.849879689659826 21.44386370723398 52-53 20.991278748421855 27.906259636139374 29.61618347791179 21.48627813752698 54-55 20.932792105425428 27.658467971337725 29.820238340389537 21.58850158284731 56-57 20.88405502079274 27.985110652281385 29.42844679675459 21.702387530171286 58-59 20.967293562348573 27.57325188225729 29.829866971255232 21.629587584138907 60-61 20.90053098992108 27.43444048318338 30.010526744328065 21.65450178256748 62-63 20.799546811804763 27.48872554778658 30.12480123579861 21.58692640461005 64-65 20.77345482926557 27.48872607234622 30.090365895220227 21.64745320316798 66-67 20.686402233337212 27.67081540072118 29.852623008026058 21.79015935791555 68-69 20.711081953788746 27.642653750593226 29.84224802955436 21.80401626606367 70-71 20.542643774427695 27.53861902103108 29.82761027359017 22.091126930951052 72-73 20.56600722834666 27.41467375309857 29.808380182651266 22.2109388359035 74-75 20.574173810880918 27.597915479195507 29.61415426849838 22.213756441425197 76-77 20.6491000103534 27.381718222332864 29.798480958468907 22.170700808844828 78-79 20.635601994355685 27.305595689288133 29.703149974523807 22.35565234183238 80-81 20.68309672376007 27.453806459418082 29.6179581315056 22.245138685316245 82-83 20.5257978513035 27.34272127898158 29.66275980476547 22.468721064949445 84-85 20.742396679988275 27.114553431438686 29.719943798007808 22.42310609056523 86-87 20.59380121407527 27.265050613609322 29.69436428539027 22.446783886925136 88-89 20.53421457917487 26.988386538434696 29.514058523285087 22.963340359105356 90-91 20.412329402897054 26.95018384298727 29.924822699985228 22.712664054130446 92-93 20.46590837709125 26.93979205587364 29.94425664278884 22.650042924246268 94-95 20.254740025590323 26.82807956263813 29.735954402698617 23.181226009072933 96-97 20.327411557234786 26.765947150707497 29.64618240869561 23.260458883362105 98-99 20.259940883398844 26.920155364290643 29.53457018322424 23.285333569086276 100-101 20.72856533021913 26.182220428350263 30.101649728922425 22.987564512508186 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 647.0 1 371.5 2 91.5 3 103.0 4 98.5 5 92.5 6 150.5 7 303.5 8 504.0 9 634.5 10 617.0 11 590.0 12 625.0 13 688.5 14 775.5 15 833.5 16 853.5 17 882.0 18 938.0 19 1011.0 20 1107.5 21 1237.5 22 1431.5 23 1738.0 24 2131.5 25 2499.0 26 3088.0 27 3944.0 28 4621.0 29 5377.5 30 6492.0 31 7295.0 32 8094.0 33 9082.0 34 10310.5 35 11455.0 36 12511.5 37 13743.0 38 14373.0 39 14640.0 40 14945.5 41 15285.0 42 15479.0 43 15778.0 44 16204.0 45 16432.0 46 16288.5 47 16150.0 48 16761.0 49 16718.5 50 15570.0 51 14361.0 52 13108.5 53 12132.5 54 11336.0 55 10157.0 56 8787.0 57 7562.0 58 6730.0 59 6037.0 60 5107.0 61 3998.5 62 2898.0 63 2101.0 64 1487.5 65 1042.5 66 773.0 67 589.0 68 403.5 69 238.0 70 152.5 71 116.5 72 86.0 73 61.5 74 46.5 75 41.0 76 34.0 77 27.0 78 18.0 79 7.5 80 7.5 81 6.0 82 3.0 83 2.5 84 1.5 85 2.0 86 4.0 87 2.0 88 0.0 89 0.0 90 0.5 91 0.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2890367993759591 2 0.2537487724641954 3 0.25653466932565044 4 0.25421308860777125 5 0.25490956282313504 6 0.2242646973471297 7 0.22217527470103846 8 0.22403253927534178 9 0.23819418165440484 10-11 0.24388205441320887 12-13 0.23610475900831357 14-15 0.22739883131626662 16-17 0.22182703759335656 18-19 0.20987089689627875 20-21 0.21567484869097672 22-23 0.21463013736793107 24-25 0.2145140583320371 26-27 0.21079952918343042 28-29 0.22345214409587197 30-31 0.2369173122595713 32-33 0.22739883131626662 34-35 0.22496117156249346 36-37 0.2166034809781284 38-39 0.21045129207574853 40-41 0.20580813063999018 42-43 0.21381758411667334 44-45 0.21277287279362775 46-47 0.22066624723441697 48-49 0.22635411999322097 50-51 0.2365690751518894 52-53 0.24225694791069347 54-55 0.22693451517269078 56-57 0.20975481786038477 58-59 0.20232575956317136 60-61 0.2051116564246264 62-63 0.20836186942965726 64-65 0.20371870799389888 66-67 0.20685284196303577 68-69 0.20638852581945996 70-71 0.209174422680915 72-73 0.2102191340039606 74-75 0.21033521303985458 76-77 0.2160230857986586 78-79 0.2154426906191888 80-81 0.2133532679730975 82-83 0.20871010653733912 84-85 0.20035241595297407 86-87 0.2011649692042318 88-89 0.20778147425018745 90-91 0.20673676292714185 92-93 0.21219247761415794 94-95 0.20685284196303577 96-97 0.21242463568594583 98-99 0.21184424050647607 100-101 0.20487949835283847 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 430741.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.43404390382469 #Duplication Level Percentage of deduplicated Percentage of total 1 86.12667689018349 62.38503495154073 2 8.525020390200638 12.350034024495873 3 2.2696916797825804 4.93208840344551 4 0.9783038193546318 2.8345000720965112 5 0.567842127184761 2.0565550785471087 6 0.3544140147996126 1.5402984184875548 7 0.23890355009414313 1.2113325165409121 8 0.17169759476322088 0.9949400893808193 9 0.1195525511173969 0.7793707262806595 >10 0.6193564956328599 7.756776830817892 >50 0.020844467995501388 1.014386315828588 >100 0.006413682409287364 0.7449363164874825 >500 3.20684120464368E-4 0.15795344409463294 >1k 9.620523613931038E-4 1.2417928119557466 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2287 0.5309455101789706 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1846 0.42856380052049836 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1213 0.2816077410787457 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 680 0.15786748881578488 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.3215807178791895E-4 0.0 0.0 0.0 0.0 3 2.3215807178791895E-4 0.0 0.0 0.0 0.0 4 2.3215807178791895E-4 0.0 0.0 0.0 0.0 5 2.3215807178791895E-4 0.0 0.0 0.0 0.0 6 2.3215807178791895E-4 0.0 0.0 0.0 0.0 7 2.3215807178791895E-4 0.0 0.0 0.0 0.0 8 2.3215807178791895E-4 0.0 0.0 0.0 0.0 9 2.3215807178791895E-4 0.0 0.0 0.0 0.0 10-11 2.3215807178791895E-4 0.0 0.0 0.0 0.0 12-13 2.3215807178791895E-4 0.0 0.0 0.0 0.0 14-15 2.3215807178791895E-4 0.0 0.0 0.0 0.0 16-17 2.3215807178791895E-4 0.0 0.0 0.0 0.0 18-19 2.3215807178791895E-4 0.0 0.0 0.0 0.0 20-21 2.3215807178791895E-4 0.0 0.0 1.1607903589395947E-4 0.0 22-23 2.3215807178791895E-4 0.0 0.0 5.803951794697974E-4 0.0 24-25 2.3215807178791895E-4 0.0 0.0 0.0011607903589395947 0.0 26-27 2.3215807178791895E-4 0.0 0.0 0.00220550168198523 0.0 28-29 2.3215807178791895E-4 0.0 0.0 0.004178845292182541 0.0 30-31 2.3215807178791895E-4 0.0 0.0 0.010098876122774474 0.0 32-33 2.3215807178791895E-4 0.0 0.0 0.015786748881578487 0.0 34-35 2.3215807178791895E-4 0.0 0.0 0.0226354119993221 0.0 36-37 4.643161435758379E-4 0.0 0.0 0.03110918161958114 0.0 38-39 4.643161435758379E-4 0.0 0.0 0.0452708239986442 0.0 40-41 4.643161435758379E-4 0.0 0.0 0.07243331839783071 0.0 42-43 4.643161435758379E-4 0.0 0.0 0.09576520461251657 0.0 44-45 4.643161435758379E-4 0.0 0.0 0.12617791201673395 0.0 46-47 4.643161435758379E-4 0.0 0.0 0.19060177693788147 0.0 48-49 6.964742153637569E-4 0.0 0.0 0.23006864914182767 0.0 50-51 6.964742153637569E-4 0.0 0.0 0.27882184421729067 0.0 52-53 6.964742153637569E-4 0.0 0.0 0.3388347057744677 0.0 54-55 6.964742153637569E-4 0.0 0.0 0.4261261407667253 0.0 56-57 6.964742153637569E-4 0.0 0.0 0.6613022674878871 0.0 58-59 6.964742153637569E-4 0.0 0.0 0.8696641369175444 0.0 60-61 6.964742153637569E-4 0.0 0.0 1.0231206223693587 0.0 62-63 6.964742153637569E-4 0.0 0.0 1.2389115500962293 0.0 64-65 6.964742153637569E-4 0.0 0.0 1.4475055775976746 0.0 66-67 6.964742153637569E-4 0.0 0.0 1.770669613526458 0.0 68-69 6.964742153637569E-4 0.0 0.0 2.1237820407158825 0.0 70-71 6.964742153637569E-4 0.0 0.0 2.3532702946782407 0.0 72-73 6.964742153637569E-4 0.0 0.0 2.646485939346382 0.0 74-75 6.964742153637569E-4 0.0 0.0 3.113704058819569 0.0 76-77 6.964742153637569E-4 0.0 0.0 3.667052822926074 0.0 78-79 6.964742153637569E-4 0.0 0.0 4.200087755751136 0.0 80-81 6.964742153637569E-4 0.0 0.0 4.597658453687947 0.0 82-83 6.964742153637569E-4 0.0 0.0 5.217404426325796 0.0 84-85 8.125532512577164E-4 0.0 0.0 5.958801228580516 0.0 86-87 9.286322871516758E-4 0.0 0.0 6.581797414223397 0.0 88-89 9.286322871516758E-4 0.0 0.0 7.34385628486724 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1330 0.0 44.3295 1 GTATCAA 2415 0.0 40.95142 1 ATCAACG 3035 0.0 32.56299 3 TATCAAC 3070 0.0 32.505066 2 TCAACGC 3085 0.0 32.031498 4 GTACACG 60 0.00392917 31.697973 1 CAACGCA 3120 0.0 31.675854 5 GTATAGG 140 1.6843842E-8 30.565905 1 AACGCAG 3210 0.0 30.481075 6 CGCAGAG 3545 0.0 27.067848 8 ACGCAGA 3625 0.0 26.732573 7 TACATGG 2130 0.0 25.655964 2 GCAGAGT 3655 0.0 25.603388 9 CATGGGG 750 0.0 25.337738 4 GTACATG 2255 0.0 24.88045 1 GAGTACT 2050 0.0 24.21199 12-13 TACCTGG 315 0.0 24.136793 2 ACATGGG 2190 0.0 24.082327 3 TATATGG 235 4.6075E-9 22.243082 2 AGTACTT 2125 0.0 22.23987 12-13 >>END_MODULE