FastQCFastQC Report
Thu 26 May 2016
SRR937299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430741
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.6869557344204522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.6008250897871343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.361005801630214No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6160.14300937222135807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19250.045.40361
GTATCAA30600.034.9272421
GCGACGA550.002576096434.5502249
CTGTTCG600.003945564431.6710389
GTGCGGG600.003947808431.6673621
ATAGACG650.00584275429.228023
TATCAAC38300.027.9020752
ATCAACG38500.027.757133
TCAACGC38900.027.5938034
CAACGCA39050.027.487815
GGACCGA700.00838969627.1403066
AACGCAG40250.026.6682996
GCATACT908.9372805E-426.389471
TACCTGG3600.026.3864042
GAGTACT25950.024.71439412-13
CTATACT1951.5656042E-824.3566824
GTACCTG4350.024.0235181
AATTACG500.00165421623.74776680-81
ACGCAGA45400.023.5447
CGCAGAG45800.023.234658
Produced by FastQC (version 0.11.5)