##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937299_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 430741 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48439085204334 33.0 31.0 34.0 31.0 34.0 2 32.68971609389401 34.0 31.0 34.0 31.0 34.0 3 32.74559189861193 34.0 31.0 34.0 31.0 34.0 4 36.311428445399905 37.0 37.0 37.0 35.0 37.0 5 36.264708955033306 37.0 37.0 37.0 35.0 37.0 6 36.3214391014554 37.0 37.0 37.0 35.0 37.0 7 36.27103990565096 37.0 37.0 37.0 35.0 37.0 8 36.25320087941478 37.0 37.0 37.0 35.0 37.0 9 37.99580026048136 39.0 38.0 39.0 35.0 39.0 10-11 37.9238765290511 39.0 38.0 39.0 35.0 39.0 12-13 37.836419101037514 39.0 38.0 39.0 35.0 39.0 14-15 39.12539670010517 40.0 38.0 41.0 36.0 41.0 16-17 39.075668441128194 40.0 38.0 41.0 36.0 41.0 18-19 39.08091289196989 40.0 38.5 41.0 36.0 41.0 20-21 38.998964574999825 40.0 38.0 41.0 36.0 41.0 22-23 38.94789095999684 40.0 38.0 41.0 35.0 41.0 24-25 38.97795543029338 40.0 38.0 41.0 35.0 41.0 26-27 38.84032051743391 40.0 38.0 41.0 35.0 41.0 28-29 38.70008659496078 40.0 38.0 41.0 35.0 41.0 30-31 38.73218360917581 40.0 38.0 41.0 35.0 41.0 32-33 38.60859542044987 40.0 38.0 41.0 35.0 41.0 34-35 38.47816669413871 40.0 38.0 41.0 35.0 41.0 36-37 38.329731787779664 40.0 38.0 41.0 34.5 41.0 38-39 38.21313387859526 40.0 38.0 41.0 34.0 41.0 40-41 38.01699977480667 40.0 38.0 41.0 34.0 41.0 42-43 37.806556608263435 40.0 37.5 41.0 33.5 41.0 44-45 37.465676589876516 40.0 37.0 41.0 33.0 41.0 46-47 37.0266389779473 40.0 37.0 41.0 32.5 41.0 48-49 36.619303711511094 40.0 37.0 41.0 31.0 41.0 50-51 36.345053059727306 40.0 36.0 41.0 30.5 41.0 52-53 36.09898407627786 39.0 36.0 41.0 30.0 41.0 54-55 35.88918863075491 39.0 36.0 41.0 29.0 41.0 56-57 35.61338484147086 39.0 35.0 40.0 28.5 41.0 58-59 35.364961775173484 39.0 35.0 40.0 28.0 41.0 60-61 35.03348764106505 38.0 35.0 40.0 27.5 41.0 62-63 34.671714557007576 38.0 34.0 40.0 26.5 41.0 64-65 34.262148251501486 37.5 34.0 40.0 26.0 41.0 66-67 33.835334922842264 37.0 34.0 39.5 25.5 41.0 68-69 33.38624370561428 36.0 33.0 39.0 24.0 40.0 70-71 32.93925235814561 36.0 33.0 39.0 22.5 40.0 72-73 32.47312770319055 35.0 32.0 38.0 21.5 40.0 74-75 31.995318532482397 35.0 32.0 37.0 20.0 39.0 76-77 30.69305452696632 34.0 30.5 36.0 19.5 38.5 78-79 31.28583650035636 34.5 31.0 36.0 19.5 39.0 80-81 31.09856387016792 35.0 31.0 36.0 18.0 38.0 82-83 30.713731685630112 34.0 31.0 35.5 15.5 37.0 84-85 30.294606271518152 34.0 31.0 35.0 9.0 37.0 86-87 29.935743288890542 34.0 30.5 35.0 7.0 36.0 88-89 29.52731107556513 34.0 30.0 35.0 2.0 36.0 90-91 29.017041563259593 34.0 29.0 35.0 2.0 35.5 92-93 28.55673478958353 34.0 29.0 35.0 2.0 35.0 94-95 28.11536747140393 34.0 29.0 35.0 2.0 35.0 96-97 27.494610450363446 33.0 27.0 35.0 2.0 35.0 98-99 26.58170571178504 33.0 25.5 34.5 2.0 35.0 100-101 23.688368416287283 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19094527910427672 1101 2 0.24417745112005917 1101 3 0.013193231394240001 1101 4 -0.04729013526423387 1101 5 0.06523148829869996 1101 6 0.09504027041005969 1101 7 0.08167671649356123 1101 8 0.09343965968766099 1101 9 0.2459828316207151 1101 10-11 0.13656388378965545 1101 12-13 0.2260242892604225 1101 14-15 0.2329508437488812 1101 16-17 0.12040327749480184 1101 18-19 0.13621815594833464 1101 20-21 0.18530934278336986 1101 22-23 0.20862511061062605 1101 24-25 0.11667249759739207 1101 26-27 -0.0019826799430759934 1101 28-29 -0.06148575238724163 1101 30-31 0.04443960395762048 1101 32-33 0.2103430672115465 1101 34-35 0.17264282782026186 1101 36-37 0.1444538920093663 1101 38-39 0.2600163486150322 1101 40-41 0.45085237324541794 1101 42-43 0.32989889034173103 1101 44-45 0.25002468956656543 1101 46-47 0.4236483686521879 1101 48-49 0.40028285900109495 1101 50-51 0.3466142069979412 1101 52-53 0.4188336678696416 1101 54-55 0.23614021438173438 1101 56-57 0.3317999897526036 1101 58-59 0.4390380518690975 1101 60-61 0.4172315645175857 1101 62-63 0.47134879680466213 1101 64-65 0.3645763152511634 1101 66-67 0.2512074603979926 1101 68-69 0.3791043500434981 1101 70-71 0.3101687672852691 1101 72-73 0.5734016315637867 1101 74-75 0.5873303640558589 1101 76-77 0.603340701785136 1101 78-79 0.621660304150204 1101 80-81 0.42493032572053835 1101 82-83 0.35728676206071697 1101 84-85 0.6697750110550018 1101 86-87 0.8647796948475488 1101 88-89 1.025608508791855 1101 90-91 0.8212070399460849 1101 92-93 0.6508128054554128 1101 94-95 1.2262137892591056 1101 96-97 1.2492942643353153 1101 98-99 1.697695076104285 1101 100-101 2.0096937112441964 1102 1 0.055794582880501764 1102 2 0.10193066879143942 1102 3 -0.004238920249548528 1102 4 0.026194722877782795 1102 5 0.0449545284829469 1102 6 0.026314464026981454 1102 7 0.03514423808988454 1102 8 0.01570564818945286 1102 9 0.08611268574728115 1102 10-11 -0.07179228874296228 1102 12-13 0.014620411483065254 1102 14-15 -0.012844619005164759 1102 16-17 0.09341584224127786 1102 18-19 0.08906848801967016 1102 20-21 0.012315686769326817 1102 22-23 -0.05395981934208294 1102 24-25 -0.006023930139384959 1102 26-27 -0.12143700366906529 1102 28-29 -0.09657207393767209 1102 30-31 0.1267308324303471 1102 32-33 0.14277515511803074 1102 34-35 -0.030555934826281828 1102 36-37 0.00580401799676622 1102 38-39 0.06477306333962218 1102 40-41 0.18060197063814343 1102 42-43 0.30518900128712545 1102 44-45 0.40970681040398915 1102 46-47 0.31237791429021655 1102 48-49 0.3317769463041955 1102 50-51 0.28752972958006495 1102 52-53 0.40033710331012884 1102 54-55 0.31499387778929844 1102 56-57 0.11943474102841378 1102 58-59 0.20269483075590244 1102 60-61 0.22473960604030907 1102 62-63 0.04418778363653786 1102 64-65 0.2776864405586963 1102 66-67 0.4227351585041461 1102 68-69 0.39339319049204136 1102 70-71 0.21705069606095861 1102 72-73 0.39109276910463464 1102 74-75 0.5515195618581927 1102 76-77 0.31092883984862496 1102 78-79 0.5333267796049697 1102 80-81 0.4396483306580521 1102 82-83 0.3023313994167225 1102 84-85 0.22577566542461724 1102 86-87 0.3666939384345156 1102 88-89 0.2737569402168951 1102 90-91 0.5443885085384714 1102 92-93 0.5366465530811979 1102 94-95 0.45702673727921095 1102 96-97 0.8336643910875594 1102 98-99 1.1475444436178073 1102 100-101 1.8223931558798512 1103 1 0.032164889311232514 1103 2 -0.009925633595948113 1103 3 -0.06842695832055057 1103 4 0.012008956346647892 1103 5 -0.06710621249336413 1103 6 -0.01727638621039773 1103 7 -0.06463315100218381 1103 8 -0.14636872202611784 1103 9 -0.16476185710531865 1103 10-11 0.009500444600966773 1103 12-13 -0.09039571645005395 1103 14-15 0.024965162245543127 1103 16-17 0.02407992692427996 1103 18-19 -0.053445084431096745 1103 20-21 0.056860583754996696 1103 22-23 -0.029378918449367575 1103 24-25 -0.009911102753697776 1103 26-27 -0.019172278411822674 1103 28-29 -0.09699514823974198 1103 30-31 -0.05994715407670981 1103 32-33 -0.20655360484835228 1103 34-35 -0.03457088155867183 1103 36-37 0.1233079364030516 1103 38-39 0.2397639771801252 1103 40-41 0.32835996077965746 1103 42-43 0.28497513580771283 1103 44-45 0.255951954987502 1103 46-47 0.1547813170569512 1103 48-49 0.3346731596472239 1103 50-51 -0.030491532033181556 1103 52-53 -0.10772402382796287 1103 54-55 0.057492117345894656 1103 56-57 0.14638656166762587 1103 58-59 -0.02943666868757333 1103 60-61 -0.1091767558614265 1103 62-63 -0.3189774936049048 1103 64-65 -0.12317788036445876 1103 66-67 0.03694818351380036 1103 68-69 -0.16745852338479494 1103 70-71 -0.06323176992273005 1103 72-73 -0.15153835553476114 1103 74-75 -0.224239700345656 1103 76-77 -0.21075016885137288 1103 78-79 -0.14519353931923007 1103 80-81 0.08551242442721829 1103 82-83 -0.04700587882419782 1103 84-85 -0.06988796495041427 1103 86-87 -0.006812524096432071 1103 88-89 0.2686686908926781 1103 90-91 0.38573280583760905 1103 92-93 0.6079643869407718 1103 94-95 0.7801447664912118 1103 96-97 0.5847320148296937 1103 98-99 0.7169428262814144 1103 100-101 1.193894984469452 1104 1 0.14118744570220798 1104 2 0.16488639647923975 1104 3 0.2605204101004972 1104 4 0.17930218942935738 1104 5 0.12650281006303032 1104 6 0.18009203484223235 1104 7 0.16844955576473808 1104 8 0.1976162403799009 1104 9 0.301403556428518 1104 10-11 0.19935006866111848 1104 12-13 0.2705065391890429 1104 14-15 0.3351155381853914 1104 16-17 0.40095962617240843 1104 18-19 0.2548255922606373 1104 20-21 0.14238689954446926 1104 22-23 0.3070872469641728 1104 24-25 0.16678062656961146 1104 26-27 0.12932396218968734 1104 28-29 -0.012408682074330102 1104 30-31 0.13930096622404164 1104 32-33 0.08292007936217516 1104 34-35 0.037797539493958254 1104 36-37 0.4193605679819967 1104 38-39 0.545215104999663 1104 40-41 0.6084351487495852 1104 42-43 0.6421179929505669 1104 44-45 0.37813240611531995 1104 46-47 0.5316610163050726 1104 48-49 0.7425678964893265 1104 50-51 0.8482678664630612 1104 52-53 0.9778022919615168 1104 54-55 0.6561763278722097 1104 56-57 0.6379279150510797 1104 58-59 0.5786460380793486 1104 60-61 0.9725901614318033 1104 62-63 0.966736792109387 1104 64-65 0.8100927963272753 1104 66-67 1.0190910406566616 1104 68-69 1.1285941081941573 1104 70-71 1.0298133428592209 1104 72-73 1.0900029978487034 1104 74-75 1.1178655628122414 1104 76-77 1.0082347935546458 1104 78-79 0.7814981900040792 1104 80-81 1.1212267101415065 1104 82-83 0.9482948730555023 1104 84-85 0.7393225613653769 1104 86-87 0.9941273255276251 1104 88-89 1.0224280893889173 1104 90-91 1.0126125050857304 1104 92-93 1.0224380711512957 1104 94-95 0.8666109319047486 1104 96-97 0.8582282554312002 1104 98-99 1.376717262371642 1104 100-101 1.8094964882288487 1105 1 0.07101069903387014 1105 2 0.025105838612759612 1105 3 0.041768938519666676 1105 4 -0.06786452694404232 1105 5 -0.09910819029799711 1105 6 -0.054556433928858894 1105 7 -0.06930296750586251 1105 8 -0.16269345871125296 1105 9 0.07144844945440099 1105 10-11 -0.10653076076108192 1105 12-13 -0.09267764779198018 1105 14-15 0.0602163905250066 1105 16-17 0.2849323450259078 1105 18-19 0.1771437833542393 1105 20-21 0.2754393898516554 1105 22-23 0.10627140255147083 1105 24-25 0.03637305754266151 1105 26-27 0.0333258772068703 1105 28-29 0.2385782581363216 1105 30-31 0.04889685050677883 1105 32-33 0.32549153932732366 1105 34-35 0.18471820755241453 1105 36-37 0.1907279216479978 1105 38-39 0.10781811565784238 1105 40-41 0.16483397462429394 1105 42-43 -0.09648019877090519 1105 44-45 0.3774849105496827 1105 46-47 0.3380166758087384 1105 48-49 0.25926150943610793 1105 50-51 0.09814032945770634 1105 52-53 0.19339477002514371 1105 54-55 0.4184571604337748 1105 56-57 0.7936505985866376 1105 58-59 0.5602924819552086 1105 60-61 0.778115849346797 1105 62-63 0.7204392894801543 1105 64-65 0.7402358732261973 1105 66-67 0.6804567461521316 1105 68-69 0.5981421955314943 1105 70-71 0.7797505554104305 1105 72-73 0.7477348797455114 1105 74-75 0.6740132388917885 1105 76-77 0.6037972906114817 1105 78-79 0.7424640179350774 1105 80-81 0.5751348364070665 1105 82-83 0.48707397111380146 1105 84-85 0.5250348849718875 1105 86-87 0.6238226494323769 1105 88-89 0.9207398918596041 1105 90-91 0.969479489875468 1105 92-93 0.4570370948064699 1105 94-95 0.6842545974360092 1105 96-97 0.9410586351382939 1105 98-99 1.3338275485254378 1105 100-101 1.7261579226081203 1106 1 -0.031479799333453684 1106 2 -0.04224200143863044 1106 3 -0.1460488977845955 1106 4 0.10283698796415308 1106 5 0.1022595095039378 1106 6 -0.08183682577083573 1106 7 0.049233245758948385 1106 8 0.07319460937932121 1106 9 0.026871071369711785 1106 10-11 -0.1240184487863516 1106 12-13 -0.35049307523162554 1106 14-15 0.009349199261158958 1106 16-17 -0.0821351299486679 1106 18-19 -0.08958109754079402 1106 20-21 -0.09112958397201254 1106 22-23 0.027819365722606904 1106 24-25 -0.08723180836387456 1106 26-27 0.2956411975858728 1106 28-29 0.1750310711548977 1106 30-31 0.28490536182843584 1106 32-33 0.27393849077532195 1106 34-35 -0.14329220409578625 1106 36-37 0.4755828544674401 1106 38-39 0.6541051184949467 1106 40-41 0.4111872212384995 1106 42-43 0.5776539482233645 1106 44-45 0.4924446289801736 1106 46-47 0.7534655275832662 1106 48-49 0.6917196219568424 1106 50-51 0.515573148899918 1106 52-53 0.3858898184700976 1106 54-55 0.9261421077064114 1106 56-57 0.6418319057971758 1106 58-59 0.613866300273294 1106 60-61 0.7354588716689392 1106 62-63 0.5345385079374125 1106 64-65 0.5748773538749887 1106 66-67 0.6886736510287434 1106 68-69 0.8180581529213597 1106 70-71 1.1823220183942098 1106 72-73 1.1415139868596889 1106 74-75 1.0386651579539432 1106 76-77 0.8691972959645149 1106 78-79 0.812284289869126 1106 80-81 1.2311891161565427 1106 82-83 0.8082090816539207 1106 84-85 0.6084786246003873 1106 86-87 0.636863974843223 1106 88-89 0.820541160528304 1106 90-91 0.6497462047194276 1106 92-93 0.7172279304148397 1106 94-95 0.5590873151706042 1106 96-97 0.7225645770043627 1106 98-99 0.8930320878969944 1106 100-101 1.792157467600358 1107 1 0.16469615115283887 1107 2 0.11989327707595976 1107 3 0.06957886525695756 1107 4 0.1447246979553114 1107 5 0.23378028757644387 1107 6 0.2089456675978667 1107 7 0.02171335185974499 1107 8 0.11397710274802364 1107 9 0.1411504701318691 1107 10-11 0.10945856270790699 1107 12-13 0.15459092123168716 1107 14-15 0.1943624618606279 1107 16-17 0.08326053760217178 1107 18-19 0.08288795639378321 1107 20-21 0.31967943459562775 1107 22-23 0.03284810135478722 1107 24-25 0.14768198473957028 1107 26-27 0.24043562729409018 1107 28-29 0.19614070864384558 1107 30-31 0.18974621063480868 1107 32-33 0.26968340677886005 1107 34-35 -0.009616253609486591 1107 36-37 0.37467657683702527 1107 38-39 0.23274128118283244 1107 40-41 0.1464878401598071 1107 42-43 -0.06779255929210848 1107 44-45 0.11120015506328684 1107 46-47 -0.20939919390214357 1107 48-49 -0.3756985920130944 1107 50-51 -0.47972968045463205 1107 52-53 -0.23524192335130323 1107 54-55 -0.3834843290752019 1107 56-57 -0.19603281568822695 1107 58-59 -0.06512353173378216 1107 60-61 -0.17203449703132634 1107 62-63 -0.01724089082474478 1107 64-65 0.15594499313228027 1107 66-67 -0.016012467450480017 1107 68-69 -0.039810989430542065 1107 70-71 0.0883233446541567 1107 72-73 0.4009635889816998 1107 74-75 0.48810149747648524 1107 76-77 0.6881075523747704 1107 78-79 0.3874942810273936 1107 80-81 0.34723686151463085 1107 82-83 0.10540183143287862 1107 84-85 0.31145165731479807 1107 86-87 0.5494413400067941 1107 88-89 0.751078295806817 1107 90-91 0.9033381587424181 1107 92-93 1.087364977276028 1107 94-95 1.2379113646843116 1107 96-97 1.452504895306749 1107 98-99 2.0795926537679037 1107 100-101 2.325037662118479 1108 1 -0.07834724006882254 1108 2 0.0178087968197147 1108 3 -0.12566912032934852 1108 4 -0.04913446825258205 1108 5 0.04759090766552987 1108 6 -0.010537841736002918 1108 7 0.0436091528707081 1108 8 0.09079257744898683 1108 9 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10-11 -0.2602363975042934 2316 12-13 -0.03826016924871567 2316 14-15 -0.32959310843114764 2316 16-17 -0.1614620530255948 2316 18-19 0.07147659476690649 2316 20-21 0.05657340748097539 2316 22-23 -0.0739434547902107 2316 24-25 -0.020458304090375634 2316 26-27 0.13404931724398494 2316 28-29 0.28534090021472736 2316 30-31 0.08324937892245998 2316 32-33 -0.027877748540916514 2316 34-35 0.2496030950495154 2316 36-37 0.014311486946070318 2316 38-39 -0.003213257573428052 2316 40-41 -0.045543547992274114 2316 42-43 -0.7415234022039812 2316 44-45 -0.6413694735839286 2316 46-47 -0.3020900279722909 2316 48-49 -0.41164576265854436 2316 50-51 -1.106488816912048 2316 52-53 -0.6018238567435858 2316 54-55 -0.12220190938761277 2316 56-57 -0.05555057283530118 2316 58-59 -0.47881532365833124 2316 60-61 -0.39060950439944264 2316 62-63 -0.49564311599421274 2316 64-65 -0.059738030740398074 2316 66-67 -0.5655998532447626 2316 68-69 -0.23082005220684465 2316 70-71 0.021798514120710877 2316 72-73 -0.37825097040526856 2316 74-75 -0.2730335599947793 2316 76-77 0.10958191134912809 2316 78-79 0.23562830696314307 2316 80-81 0.1374391161565427 2316 82-83 0.14020694490178442 2316 84-85 -0.06994017881841685 2316 86-87 -0.2293219225926748 2316 88-89 -0.3298327710956279 2316 90-91 -0.0583200345968109 2316 92-93 0.21001639195329957 2316 94-95 0.5641621014953913 2316 96-97 1.0286543205941037 2316 98-99 1.1419637118286197 2316 100-101 1.6407151599080514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 2.0 5 12.0 6 12.0 7 30.0 8 59.0 9 138.0 10 275.0 11 437.0 12 743.0 13 1187.0 14 1546.0 15 2299.0 16 3457.0 17 5462.0 18 5170.0 19 2753.0 20 1982.0 21 2051.0 22 1982.0 23 2098.0 24 2396.0 25 2690.0 26 3186.0 27 3894.0 28 4875.0 29 5971.0 30 7727.0 31 9873.0 32 13075.0 33 18264.0 34 26139.0 35 39758.0 36 65635.0 37 104276.0 38 84169.0 39 7115.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.307234750213105 20.322752219850564 15.425958141619848 20.94405488831649 2 15.461263264931826 21.381990569739123 40.42707798886106 22.729668176467992 3 19.040444257686172 26.112907756633337 30.975922886374875 23.870725099305616 4 12.590164391130635 18.008501628588874 37.996150819169756 31.405183161110738 5 12.882368782734668 38.63178373760262 34.34075628691356 14.145091192749154 6 30.261108183339875 37.90003737744956 17.55231101752561 14.286543421684957 7 26.978625206330488 34.77913641840457 21.009841180663088 17.232397194601862 8 26.293062420340764 35.57450997234997 19.59971305262327 18.532714554685995 9 26.759003670419112 16.895535832437588 20.662068389124787 35.68339210801851 10-11 24.28396646708811 28.07325051481052 27.179441938427036 20.46334107967433 12-13 25.549379749392287 25.485880389029354 28.299354790777155 20.665385070801207 14-15 22.56982734404201 26.456849939987137 27.16075321364811 23.81256950232274 16-17 21.06997012125616 30.020824579039378 27.698199149837144 21.211006149867323 18-19 20.760039095419287 27.791410615659988 31.204018191906506 20.244532097014215 20-21 21.701904392662875 26.85500103310342 31.54772821718852 19.895366357045184 22-23 21.532305713585917 26.95638748979945 31.286064682236127 20.22524211437851 24-25 21.08169410388145 26.993251164853127 31.550398035014084 20.374656696251346 26-27 21.090046293071826 27.308733464272617 31.341152544261657 20.260067698393904 28-29 20.721061649401243 27.401874973881306 31.623584366859674 20.253479009857774 30-31 20.973068471620383 27.29265067946943 31.368538598065427 20.365742250844765 32-33 20.82971069588302 27.460269001870035 31.120361331242364 20.589658971004585 34-35 20.9731602053206 27.38234809316968 31.253235703125544 20.39125599838418 36-37 21.314664728920626 27.28333267555213 30.95084981462178 20.451152780905463 38-39 21.066023434035767 27.40092073891271 31.166408584276862 20.36664724277466 40-41 21.14263559772578 27.606264553409126 30.983003707564404 20.26809614130069 42-43 21.093465213201917 27.708819466211587 30.660676348692228 20.537038971894265 44-45 21.29040421041879 27.700985046698595 30.43453026296545 20.574080479917168 46-47 21.379088593841775 28.077893676246283 29.831151434388648 20.711866295523297 48-49 21.585361040625344 28.148934046213387 29.42487480875979 20.840830104401483 50-51 21.61705061742439 28.445515982922455 29.200261874304978 20.73717152534818 52-53 21.603933686368375 28.820219110788152 28.83101446112629 20.744832741717183 54-55 21.380829779380186 28.69926475538665 28.891491638827045 21.028413826406123 56-57 21.521517570883663 28.868740147791826 28.742794393846882 20.86694788747763 58-59 21.60428192347606 28.383878014862763 29.038447698268797 20.97339236339239 60-61 21.37630269698032 28.43634573908683 29.337931610875213 20.849419953057637 62-63 21.385589019851835 28.362055156114696 29.406302163016758 20.846053661016715 64-65 21.391044734538852 28.40187026542632 29.28070464617949 20.926380353855333 66-67 20.99022382359701 28.742330077703304 29.112622202205035 21.154823896494644 68-69 21.18779034268853 28.49380486185434 29.077450254329168 21.240954541127962 70-71 21.10514206913203 28.58306964045679 29.055511316545207 21.256276973865965 72-73 21.14932575257203 28.4576873028826 28.93210397467611 21.46088296986926 74-75 21.185236603898865 28.54627258607841 28.685451350115265 21.58303945990746 76-77 21.28285907308568 28.24168119589266 28.898108143873003 21.57735158714866 78-79 21.26080405626583 28.323168679090223 28.729445304719075 21.686581959924876 80-81 21.017618476067984 28.667110862444023 28.51644027385366 21.798830387634332 82-83 20.865022983702467 28.5436690346845 28.637228954821936 21.954079026791103 84-85 21.107695807921697 28.379002695355215 28.731302569293383 21.781998927429708 86-87 21.00856431126826 28.316436095008367 28.557880489667802 22.11711910405557 88-89 20.838392444647713 28.248994175153978 28.36983245151959 22.542780928678717 90-91 20.686989047832437 28.310784297791226 28.661964145988655 22.340262508387685 92-93 20.69272129564281 28.167601500687194 28.50748486311801 22.632192340551985 94-95 20.533568896390175 28.17284632760754 28.475812611290774 22.81777216471151 96-97 20.760503411562865 28.130129242398567 28.188400918417333 22.920966427621238 98-99 20.59019499046987 28.31633541425318 28.087310413034345 23.006159182242612 100-101 20.286574067624237 28.608413788619696 28.013416180219842 23.091595963536225 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 151.0 1 109.5 2 75.0 3 96.5 4 98.5 5 101.5 6 163.5 7 333.0 8 569.0 9 741.0 10 786.5 11 767.0 12 812.0 13 886.0 14 942.0 15 1006.5 16 1010.0 17 1042.5 18 1125.0 19 1159.5 20 1210.0 21 1313.5 22 1512.5 23 1759.5 24 2028.5 25 2404.0 26 2953.0 27 3693.0 28 4441.5 29 5383.5 30 6354.5 31 7121.0 32 8089.5 33 8958.0 34 10096.5 35 11137.5 36 12187.0 37 13381.5 38 14018.5 39 14483.0 40 14805.5 41 15187.5 42 15383.5 43 15653.0 44 16300.0 45 16488.0 46 16144.0 47 16276.0 48 17063.5 49 16694.0 50 15596.0 51 14577.5 52 13302.0 53 12465.0 54 11664.0 55 10323.0 56 8771.0 57 7592.0 58 6913.5 59 6128.5 60 5051.0 61 3972.5 62 2929.0 63 2074.0 64 1432.0 65 1011.5 66 732.5 67 559.5 68 378.5 69 240.0 70 169.5 71 110.5 72 84.0 73 68.0 74 55.0 75 46.0 76 36.5 77 18.0 78 5.0 79 3.0 80 3.0 81 4.0 82 1.5 83 1.0 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04503866592685628 2 0.0 3 0.0 4 0.0 5 0.0011607903589395947 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00650042601006173 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010447113230456352 24-25 0.0 26-27 0.0016251065025154326 28-29 0.0034823710768187846 30-31 1.1607903589395947E-4 32-33 1.1607903589395947E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010679271302244272 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.4823710768187844E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.3215807178791895E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.011607903589395948 92-93 0.0011607903589395947 94-95 0.0 96-97 0.0 98-99 4.643161435758379E-4 100-101 0.01555459080979057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 430741.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.58567928106555 #Duplication Level Percentage of deduplicated Percentage of total 1 84.81179057350501 59.01686058100817 2 9.250047721869471 12.873417082171198 3 2.561940907805131 5.348231950427092 4 1.0651650832675739 2.9648094346258747 5 0.6288833411859891 2.1880637242486576 6 0.37556449044522366 1.5680346108886885 7 0.24427209492992424 1.1898487758575393 8 0.18447038675782532 1.0269197735827322 9 0.13711035014634693 0.8586825166258466 >10 0.6950475831317607 8.407306285905538 >50 0.03327234730830359 1.569010712108276 >100 0.011090782388257956 1.1838324933497746 >500 3.3608431479569564E-4 0.14406179689914286 >1k 0.0010082529443870868 1.6609202623014816 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2959 0.6869557344204522 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2588 0.6008250897871343 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1555 0.361005801630214 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 616 0.14300937222135807 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.1607903589395947E-4 0.0 22-23 0.0 0.0 0.0 5.803951794697974E-4 0.0 24-25 0.0 0.0 0.0 0.0011607903589395947 0.0 26-27 0.0 0.0 0.0 0.00220550168198523 0.0 28-29 0.0 0.0 0.0 0.004178845292182541 0.0 30-31 2.3215807178791895E-4 0.0 0.0 0.010331034194562393 0.0 32-33 2.3215807178791895E-4 0.0 0.0 0.015786748881578487 0.0 34-35 2.3215807178791895E-4 0.0 0.0 0.022983649107003977 0.0 36-37 2.3215807178791895E-4 0.0 0.0 0.031573497763156974 0.0 38-39 2.3215807178791895E-4 0.0 0.0 0.04619945628579587 0.0 40-41 2.3215807178791895E-4 0.0 0.0 0.07336195068498239 0.0 42-43 2.3215807178791895E-4 0.0 0.0 0.09785462725860784 0.0 44-45 2.3215807178791895E-4 0.0 0.0 0.12907988791408292 0.0 46-47 2.3215807178791895E-4 0.0 0.0 0.194780622230064 0.0 48-49 2.3215807178791895E-4 0.0 0.0 0.23447965250579816 0.0 50-51 2.3215807178791895E-4 0.0 0.0 0.2839293217966249 0.0 52-53 2.3215807178791895E-4 0.0 0.0 0.3463798431075751 0.0 54-55 2.3215807178791895E-4 0.0 0.0 0.43494814749466615 0.0 56-57 2.3215807178791895E-4 0.0 0.0 0.6730262501131771 0.0 58-59 2.3215807178791895E-4 0.0 0.0 0.8841740164042893 0.0 60-61 2.3215807178791895E-4 0.0 0.0 1.0375144228202098 0.0 62-63 2.3215807178791895E-4 0.0 0.0 1.254350061870126 0.0 64-65 2.3215807178791895E-4 0.0 0.0 1.466774697556072 0.0 66-67 2.3215807178791895E-4 0.0 0.0 1.792840709382204 0.0 68-69 2.3215807178791895E-4 0.0 0.0 2.14920334957666 0.0 70-71 2.3215807178791895E-4 0.0 0.0 2.3819418165440487 0.0 72-73 2.3215807178791895E-4 0.0 0.0 2.6832829937247675 0.0 74-75 2.3215807178791895E-4 0.0 0.0 3.159439198961789 0.0 76-77 2.3215807178791895E-4 0.0 0.0 3.7221903649757047 0.0 78-79 2.3215807178791895E-4 0.0 0.0 4.263815146456919 0.0 80-81 2.3215807178791895E-4 0.0 0.0 4.667654112332005 0.0 82-83 2.3215807178791895E-4 0.0 0.0 5.298543672415674 0.0 84-85 2.3215807178791895E-4 0.0 0.0 6.052360931511047 0.0 86-87 2.3215807178791895E-4 0.0 0.0 6.684643440025445 0.0 88-89 2.3215807178791895E-4 0.0 0.0 7.464114166053382 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1925 0.0 45.4036 1 GTATCAA 3060 0.0 34.927242 1 GCGACGA 55 0.0025760964 34.550224 9 CTGTTCG 60 0.0039455644 31.671038 9 GTGCGGG 60 0.0039478084 31.667362 1 ATAGACG 65 0.005842754 29.22802 3 TATCAAC 3830 0.0 27.902075 2 ATCAACG 3850 0.0 27.75713 3 TCAACGC 3890 0.0 27.593803 4 CAACGCA 3905 0.0 27.48781 5 GGACCGA 70 0.008389696 27.140306 6 AACGCAG 4025 0.0 26.668299 6 GCATACT 90 8.9372805E-4 26.38947 1 TACCTGG 360 0.0 26.386404 2 GAGTACT 2595 0.0 24.714394 12-13 CTATACT 195 1.5656042E-8 24.356682 4 GTACCTG 435 0.0 24.023518 1 AATTACG 50 0.001654216 23.747766 80-81 ACGCAGA 4540 0.0 23.544 7 CGCAGAG 4580 0.0 23.23465 8 >>END_MODULE