FastQCFastQC Report
Thu 26 May 2016
SRR937298_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937298_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422628
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24560.581125718125633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.46116206214448635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12560.29718807083297843No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5520.1306113177546211No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.1152313618596023No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG200.002065832571.3136445
TATACCG250.00500136757.050925
GTATCAA22550.040.2783161
GGTATCA13000.037.3113821
GGAACCG651.3198055E-436.5711025
ATCAACG28600.031.5803853
TATCAAC29000.031.1447932
TCAACGC29100.031.0414524
CAACGCA29350.030.7770445
AACGCAG29800.030.2871236
CGACTTA350.00840233727.134936-37
ACGCAGA34050.026.3672857
CGCAGAG34000.026.126638
GTACATG23300.025.307561
TACCTGG2701.8189894E-1224.6487062
AGTACTT18250.023.9452812-13
TACATGG23250.023.717282
GCAGAGT36200.023.6202589
GAGTACT17600.023.48018812-13
GTGTATC1050.002179563722.6446291
Produced by FastQC (version 0.11.5)