FastQCFastQC Report
Thu 26 May 2016
SRR937298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422628
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30070.7115004211741769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24760.5858580122471772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15570.3684090973622193No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.15592909130488278No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.043.080081
GTATCAA25700.040.8590281
CATATAG1353.074092E-1035.183653
GGACCGA550.002578338834.543956
ATCAACG32350.031.8610533
TATCAAC32850.031.6652872
TCAACGC32600.031.616724
CAACGCA33050.031.1862355
AACGCAG33900.030.404286
TAAGGTG1405.7936995E-727.1416725
TACCTGG2850.026.6655042
ACGCAGA38550.026.493557
CGCAGAG38650.026.4250018
GTCTAAG1701.11595E-725.1548921
GCAGAGT40800.024.9160779
GAGTACT23100.024.36870412-13
GCACCCG1201.7074577E-423.7517749
GTAAGGT1401.7766284E-523.7489644
AGTACTT23850.023.10445212-13
GTACTTT24800.022.69558114-15
Produced by FastQC (version 0.11.5)