##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937298_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 422628 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4670963589729 33.0 31.0 34.0 31.0 34.0 2 32.685385256064436 34.0 31.0 34.0 31.0 34.0 3 32.75065542273583 34.0 31.0 34.0 31.0 34.0 4 36.300271160453164 37.0 37.0 37.0 35.0 37.0 5 36.20488940628638 37.0 35.0 37.0 35.0 37.0 6 36.29910228380514 37.0 37.0 37.0 35.0 37.0 7 36.271219606841 37.0 37.0 37.0 35.0 37.0 8 36.25729483138836 37.0 37.0 37.0 35.0 37.0 9 38.00236378091371 39.0 38.0 39.0 35.0 39.0 10-11 37.9342353085929 39.0 38.0 39.0 35.0 39.0 12-13 37.860929706503114 39.0 38.0 39.0 35.0 39.0 14-15 39.144381110574784 40.0 38.0 41.0 36.0 41.0 16-17 39.13068585138704 40.0 38.0 41.0 36.0 41.0 18-19 39.14647160150298 40.0 38.5 41.0 36.0 41.0 20-21 39.08976097182392 40.0 38.5 41.0 36.0 41.0 22-23 38.97024096841667 40.0 38.0 41.0 35.5 41.0 24-25 38.83440164873127 40.0 38.0 41.0 35.0 41.0 26-27 38.63975292692392 40.0 38.0 41.0 35.0 41.0 28-29 38.619469131245445 40.0 38.0 41.0 35.0 41.0 30-31 38.715449520618606 40.0 38.0 41.0 35.0 41.0 32-33 38.64132878086639 40.0 38.0 41.0 35.0 41.0 34-35 38.52705215934581 40.0 38.0 41.0 35.0 41.0 36-37 38.38993275410053 40.0 38.0 41.0 35.0 41.0 38-39 38.2470056408946 40.0 38.0 41.0 34.5 41.0 40-41 38.088644150411234 40.0 38.0 41.0 34.0 41.0 42-43 37.86751114455265 40.0 38.0 41.0 33.5 41.0 44-45 37.53403939161627 40.0 37.5 41.0 33.0 41.0 46-47 37.10291201718769 40.0 37.0 41.0 32.5 41.0 48-49 36.701940003975125 40.0 37.0 41.0 31.0 41.0 50-51 36.46010912670245 40.0 37.0 41.0 31.0 41.0 52-53 36.23529794523789 40.0 36.0 41.0 30.0 41.0 54-55 36.035009511911184 39.0 36.0 41.0 30.0 41.0 56-57 35.806030362399085 39.0 35.5 41.0 29.0 41.0 58-59 35.573621482722395 39.0 35.0 40.5 28.5 41.0 60-61 35.26153969921539 38.5 35.0 40.0 28.0 41.0 62-63 34.91546229781273 38.0 35.0 40.0 27.0 41.0 64-65 34.58681038643914 38.0 34.0 40.0 26.5 41.0 66-67 34.20419020982992 37.0 34.0 40.0 26.0 41.0 68-69 33.76829623214742 36.5 34.0 39.0 25.5 41.0 70-71 33.37569919645645 36.0 33.5 39.0 25.0 40.0 72-73 32.95457352565377 35.5 33.0 38.5 23.5 40.0 74-75 32.529277520656464 35.0 33.0 37.5 22.5 39.5 76-77 31.220936615652533 34.0 30.5 36.0 20.5 39.0 78-79 31.78105213095204 35.0 32.0 37.0 21.0 39.0 80-81 31.586255524953387 35.0 32.0 36.0 20.0 38.5 82-83 31.25312094797316 35.0 32.0 36.0 19.5 37.0 84-85 30.92026321019904 35.0 32.0 35.5 18.0 37.0 86-87 30.598638755595935 34.0 31.0 35.0 15.5 36.5 88-89 30.285493388985113 34.0 31.0 35.0 8.5 36.0 90-91 29.895163122178367 34.0 31.0 35.0 4.5 36.0 92-93 29.545351940713818 34.0 30.5 35.0 2.0 35.0 94-95 29.192121676746453 34.0 30.0 35.0 2.0 35.0 96-97 28.83237859299431 34.0 30.0 35.0 2.0 35.0 98-99 28.275181720094267 34.0 29.0 35.0 2.0 35.0 100-101 26.81674900858438 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0391779255885325 1101 2 -0.005637580679227483 1101 3 0.020774366995752303 1101 4 0.012310799025279096 1101 5 -0.027822260663327825 1101 6 0.011606703975829191 1101 7 -0.048038443601008396 1101 8 0.05857000537344703 1101 9 0.0616227906365765 1101 10-11 0.08079480601372069 1101 12-13 0.06977401725386301 1101 14-15 0.08628086637711618 1101 16-17 -0.009559690268353904 1101 18-19 0.014939249870131732 1101 20-21 0.01782289087721267 1101 22-23 0.1251945057018773 1101 24-25 0.061345863444543625 1101 26-27 0.021223103232181018 1101 28-29 0.11133740760227795 1101 30-31 0.19018205359995477 1101 32-33 0.08242972323914444 1101 34-35 0.22938217054538512 1101 36-37 0.1662840526273044 1101 38-39 0.10995660603649782 1101 40-41 0.15745118656331414 1101 42-43 0.08282815465184257 1101 44-45 -0.13459066358343819 1101 46-47 -0.06048907356004918 1101 48-49 0.021113080454490785 1101 50-51 -0.1599070486586882 1101 52-53 -0.07175068453823741 1101 54-55 -0.20529018424190326 1101 56-57 -0.24850127372872066 1101 58-59 -0.038236996707524895 1101 60-61 -0.19602201350810589 1101 62-63 -0.3088009950538151 1101 64-65 -0.14648540041496005 1101 66-67 -0.13132524600576545 1101 68-69 -0.20514057666511576 1101 70-71 -0.21689199325181363 1101 72-73 -0.3658924521526856 1101 74-75 -0.294795387351126 1101 76-77 -0.06395388263765867 1101 78-79 0.015871839022221224 1101 80-81 -0.12440240760519217 1101 82-83 -0.33410440754017756 1101 84-85 -0.22835572601674414 1101 86-87 -0.30903872177839986 1101 88-89 -0.21458867836294004 1101 90-91 0.06695066118009407 1101 92-93 0.19528986906203727 1101 94-95 -0.0020491564873594825 1101 96-97 0.14221897674275752 1101 98-99 -0.13638152986544583 1101 100-101 -0.18959972749347287 1102 1 -0.11603805519451527 1102 2 -0.05720089367293468 1102 3 -0.14039114078533999 1102 4 -0.06342828616463692 1102 5 -0.1398415596558067 1102 6 -0.09764546006560693 1102 7 -0.03920253233852833 1102 8 -0.08012258248899684 1102 9 -0.09145889897128967 1102 10-11 -0.09491826337750808 1102 12-13 -0.19205371085739387 1102 14-15 -0.19776671888375574 1102 16-17 -0.1490989741243709 1102 18-19 -0.21603137592808253 1102 20-21 -0.13682447679696708 1102 22-23 -0.18299337566607932 1102 24-25 -0.10380715675317731 1102 26-27 -0.23086525588043827 1102 28-29 -0.3290745880459838 1102 30-31 -0.2643328700082037 1102 32-33 -0.13577187554994197 1102 34-35 0.04215592769973142 1102 36-37 -0.001267151194653593 1102 38-39 0.07513800710550811 1102 40-41 0.06740577716924889 1102 42-43 0.10368336490679297 1102 44-45 0.009153719369116686 1102 46-47 -0.30188730448574574 1102 48-49 -0.095709681004287 1102 50-51 -0.2786100428406044 1102 52-53 -0.246789708236264 1102 54-55 -0.4265427266949544 1102 56-57 -0.2815720840025975 1102 58-59 -0.24504840581778353 1102 60-61 -0.1679367762913202 1102 62-63 -0.13783935653150792 1102 64-65 -0.12909738660293613 1102 66-67 -0.13100596120372643 1102 68-69 -0.44010817517039413 1102 70-71 -0.46158477032007283 1102 72-73 -0.40264804554939815 1102 74-75 -0.5704162189106583 1102 76-77 -0.40463313149059843 1102 78-79 -0.5388512109754124 1102 80-81 -0.5639748024777127 1102 82-83 -0.5374557154253274 1102 84-85 -0.34186975089730964 1102 86-87 -0.3280420801815005 1102 88-89 -0.39729053731000974 1102 90-91 -0.5100608330727816 1102 92-93 -0.47881022081072544 1102 94-95 -0.31545202295536257 1102 96-97 -0.33997675666626037 1102 98-99 -0.20716578794217 1102 100-101 -0.2412505481736673 1103 1 0.16932710426782904 1103 2 0.11404431004467597 1103 3 -0.06250786084508775 1103 4 -0.07584886529232904 1103 5 -0.13249706834529462 1103 6 -0.03903076979898401 1103 7 0.03006509433361515 1103 8 0.006949871602579094 1103 9 0.07804648206699483 1103 10-11 -0.014122438951936545 1103 12-13 -0.026709566227410164 1103 14-15 0.04101484883985762 1103 16-17 -0.01989734515385777 1103 18-19 -0.0608340569242074 1103 20-21 -0.10906756405132256 1103 22-23 -0.1233001237384741 1103 24-25 -0.050987595411051245 1103 26-27 -0.015094073592827328 1103 28-29 -0.4410277904539157 1103 30-31 -0.20555018948287085 1103 32-33 0.0615481983229742 1103 34-35 0.14630346808694128 1103 36-37 0.23447133184261304 1103 38-39 0.12795649844598245 1103 40-41 0.1746199422790653 1103 42-43 0.25557009881236326 1103 44-45 0.08576904735672031 1103 46-47 0.14892150975983043 1103 48-49 0.3637126465060945 1103 50-51 0.1763760578379916 1103 52-53 0.47636020541460056 1103 54-55 0.3942758673656783 1103 56-57 0.3744020561975745 1103 58-59 0.13010808192321122 1103 60-61 0.24632292166861447 1103 62-63 0.00931904009242146 1103 64-65 0.013639763212275113 1103 66-67 0.24976482518329135 1103 68-69 0.25838355050686346 1103 70-71 0.1369848514771448 1103 72-73 0.0012807865009989428 1103 74-75 0.08868767610933759 1103 76-77 -0.10744017381344406 1103 78-79 0.21387782822730728 1103 80-81 0.26521869448027147 1103 82-83 0.39798894515945094 1103 84-85 0.29861183544433345 1103 86-87 0.37180747758681676 1103 88-89 0.14523881818519513 1103 90-91 0.2515096297457333 1103 92-93 0.2064156244370814 1103 94-95 0.3069229955021626 1103 96-97 0.3082634116235994 1103 98-99 0.09836034256807125 1103 100-101 0.5200556242378411 1104 1 0.04493556466172066 1104 2 0.10356136376226743 1104 3 0.050004128078228405 1104 4 0.03551891761621562 1104 5 -0.009980488175152402 1104 6 0.1106078197507614 1104 7 0.06179277330209487 1104 8 0.028314793996088383 1104 9 -0.004117605729184959 1104 10-11 0.017828792872371935 1104 12-13 0.07091812932725361 1104 14-15 0.12301908432514352 1104 16-17 0.09921065491344194 1104 18-19 0.0937900016899107 1104 20-21 -0.0833265344101406 1104 22-23 -0.045770551060961395 1104 24-25 -0.13454348001931749 1104 26-27 -0.0555475253210318 1104 28-29 -0.16835460309498274 1104 30-31 -0.032746235722264316 1104 32-33 0.14736605322514862 1104 34-35 0.10807352594626707 1104 36-37 0.12250139849982133 1104 38-39 0.028712251326332705 1104 40-41 0.018343275187724828 1104 42-43 0.1315715795495649 1104 44-45 0.2624250812406004 1104 46-47 0.19750819899011418 1104 48-49 0.2922153952447033 1104 50-51 0.36601258012476734 1104 52-53 0.502669732371956 1104 54-55 0.35465172461876904 1104 56-57 0.3509806879098747 1104 58-59 0.36424604528457394 1104 60-61 0.34633758481292176 1104 62-63 0.5777069836224911 1104 64-65 0.5990081086086434 1104 66-67 0.343113767153298 1104 68-69 0.4944529624667027 1104 70-71 0.3900514918623301 1104 72-73 0.5076592542745288 1104 74-75 0.5053445272832349 1104 76-77 0.4901009851518694 1104 78-79 0.5956904813189503 1104 80-81 0.6809657095688841 1104 82-83 0.6296125398412933 1104 84-85 0.5602155507485698 1104 86-87 0.5880576067907555 1104 88-89 0.7005681500025354 1104 90-91 0.6784342876257305 1104 92-93 0.6053468975585723 1104 94-95 0.3766510542701269 1104 96-97 0.30153038421313383 1104 98-99 0.5648502502383366 1104 100-101 0.6659454968127108 1105 1 0.05590545757537768 1105 2 0.18822993642927344 1105 3 0.09152049682101193 1105 4 0.1520224460919195 1105 5 0.05906897579936299 1105 6 0.10304913305030539 1105 7 0.0256975207867427 1105 8 0.08507322775069781 1105 9 0.13382397328224727 1105 10-11 0.07792124279533397 1105 12-13 -0.0454874271684389 1105 14-15 0.035251034472253195 1105 16-17 0.078673649709053 1105 18-19 0.12104847849281697 1105 20-21 -0.07754618758933418 1105 22-23 0.16267374296186432 1105 24-25 0.12237170890331583 1105 26-27 0.34353391314514425 1105 28-29 0.1031153625554424 1105 30-31 0.04149425876904189 1105 32-33 0.20584344990182757 1105 34-35 0.17592060652970787 1105 36-37 0.18547214644166843 1105 38-39 0.43936328997776286 1105 40-41 0.40406935146931033 1105 42-43 0.5178424394169738 1105 44-45 0.5322354609265645 1105 46-47 0.6522941302137824 1105 48-49 0.6936763652860662 1105 50-51 0.5612410478133114 1105 52-53 0.6600943755906599 1105 54-55 0.47841853943094037 1105 56-57 0.43303815886712727 1105 58-59 0.3623875465121955 1105 60-61 0.5274016792697793 1105 62-63 0.36702120945086847 1105 64-65 0.609185319138561 1105 66-67 0.6719270295905417 1105 68-69 0.5104298173286352 1105 70-71 0.620291582590319 1105 72-73 0.483449784243561 1105 74-75 0.509676076338998 1105 76-77 0.5430622624689789 1105 78-79 0.5442819114426634 1105 80-81 0.4075556018293973 1105 82-83 0.3699318691191813 1105 84-85 0.3279328926796907 1105 86-87 0.5581627930711122 1105 88-89 0.5137682414430493 1105 90-91 0.3781857950582399 1105 92-93 0.4712084126804008 1105 94-95 0.8816030925326359 1105 96-97 0.9776053298089984 1105 98-99 0.48088974779052407 1105 100-101 0.6144033287818615 1106 1 -0.15723066392524743 1106 2 0.07639809831385236 1106 3 0.014122263183402595 1106 4 0.03465437836683094 1106 5 0.06910393687138594 1106 6 -0.009894310222954061 1106 7 0.07286445336679748 1106 8 0.12705702883004477 1106 9 0.1709690224980207 1106 10-11 0.0077854961889940455 1106 12-13 0.09130103654963762 1106 14-15 0.284954606430162 1106 16-17 0.22079345894889002 1106 18-19 0.1623413406799301 1106 20-21 -0.2824215570345743 1106 22-23 -0.014143404528532244 1106 24-25 0.07664986302846444 1106 26-27 0.07020110292011594 1106 28-29 -0.05938605419623144 1106 30-31 -0.2750647347705737 1106 32-33 -0.027948910973165653 1106 34-35 -0.06482909486263111 1106 36-37 -0.056951501541306015 1106 38-39 -0.139013592122339 1106 40-41 0.08406220232240713 1106 42-43 -0.16438669945956264 1106 44-45 0.07960291835862421 1106 46-47 -0.2728883038091823 1106 48-49 -0.5250199616217586 1106 50-51 -0.09009636576484326 1106 52-53 -0.32084050614327 1106 54-55 -0.3100517175024251 1106 56-57 -0.43431029229635953 1106 58-59 -0.5143071064490314 1106 60-61 -0.10322282486713874 1106 62-63 -0.3329805342434895 1106 64-65 -0.14682606718564273 1106 66-67 -0.1954018050113291 1106 68-69 0.13406210706089183 1106 70-71 0.12176718394691477 1106 72-73 0.03485389413118867 1106 74-75 0.05682993121131119 1106 76-77 -0.0790290373679845 1106 78-79 -0.01830145894417612 1106 80-81 0.04331746548993465 1106 82-83 0.08126980580564336 1106 84-85 0.21154129379913655 1106 86-87 0.2555969468126449 1106 88-89 0.08094624648233051 1106 90-91 0.3606028170026363 1106 92-93 0.3553486405812514 1106 94-95 0.2727180978944972 1106 96-97 0.3565035765243145 1106 98-99 0.3642998036759266 1106 100-101 0.09207968167072522 1107 1 -0.09753976343636594 1107 2 -0.10736325939483038 1107 3 -0.022774368020122893 1107 4 0.0600689574795652 1107 5 -0.0726960704072539 1107 6 0.004220182759226532 1107 7 0.03192211231406361 1107 8 0.11551432141918383 1107 9 0.16080925638493682 1107 10-11 -0.03736025854514935 1107 12-13 0.11518710159640477 1107 14-15 0.28995563757060694 1107 16-17 0.22249787344308203 1107 18-19 0.18512044613527223 1107 20-21 0.013188210898171349 1107 22-23 0.1672918216551622 1107 24-25 0.13836968397396276 1107 26-27 0.17262367640403653 1107 28-29 0.10569043216239038 1107 30-31 0.08394803923983574 1107 32-33 0.0432937952245922 1107 34-35 0.024792173318793687 1107 36-37 0.12359815696923704 1107 38-39 0.1359157444297665 1107 40-41 0.013598916987653809 1107 42-43 -0.024151599438944515 1107 44-45 0.17687585516791415 1107 46-47 0.45563061458515364 1107 48-49 0.5157434462367476 1107 50-51 0.7803105101222414 1107 52-53 0.8034149498027716 1107 54-55 0.7923678232154927 1107 56-57 0.8300741168601675 1107 58-59 0.8251200647081518 1107 60-61 0.5439937024944825 1107 62-63 0.8609102619182352 1107 64-65 0.9009430301418817 1107 66-67 0.7670677156903309 1107 68-69 1.0760695555341968 1107 70-71 0.9518685996420899 1107 72-73 0.8017433680069388 1107 74-75 0.8762347812107052 1107 76-77 0.6976775000624116 1107 78-79 0.7542805167209075 1107 80-81 0.7230907359034866 1107 82-83 0.8132855034024793 1107 84-85 0.7867963494200652 1107 86-87 0.7281018902212324 1107 88-89 0.5669742935023301 1107 90-91 0.4975352348450066 1107 92-93 0.8126776229062926 1107 94-95 0.6950125673424097 1107 96-97 0.8790036675072948 1107 98-99 0.9530779629082744 1107 100-101 1.164520330440027 1108 1 0.11876112814041306 1108 2 0.07175482776771247 1108 3 0.08537607776827372 1108 4 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-1.1302766112738034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 3.0 5 4.0 6 12.0 7 22.0 8 65.0 9 123.0 10 251.0 11 462.0 12 817.0 13 1155.0 14 1469.0 15 2331.0 16 3363.0 17 5470.0 18 4958.0 19 2540.0 20 1955.0 21 1798.0 22 1803.0 23 1842.0 24 1975.0 25 2267.0 26 2658.0 27 3226.0 28 3981.0 29 4966.0 30 6173.0 31 8106.0 32 10963.0 33 15176.0 34 22075.0 35 33833.0 36 57422.0 37 102575.0 38 104296.0 39 12491.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.35211400975333 20.377586288528004 15.416646939065384 20.853652762653283 2 15.396518924444191 21.251549826324805 40.637865924642945 22.714065324588052 3 19.100248918670793 26.14403210388332 30.886027428376728 23.86969154906916 4 12.578437775064597 17.826788570563238 38.29490710506639 31.299866549305772 5 12.895126672440641 38.60184672469036 34.16961281253993 14.33341379032907 6 30.272722110224592 37.94826656066328 17.577396670357857 14.201614658754272 7 27.112022866445194 34.699546646223155 20.90419943780346 17.28423104952819 8 26.321729748147305 35.46310230273432 19.66078915736771 18.554378791750665 9 26.732729492603426 16.878673443311847 20.787785002413468 35.600812061671256 10-11 24.284003899410354 28.077884096652372 27.14313770029435 20.49497430364292 12-13 25.576924214548963 25.46996917943955 28.26831042989062 20.68479617612087 14-15 22.547133649450583 26.38324957172738 27.281675610702553 23.78794116811948 16-17 21.039543049679622 30.012564240892697 27.59791116537475 21.349981544052927 18-19 20.742946515611838 27.706280700758114 31.302587618425658 20.248185165204386 20-21 21.673197232554397 26.93006615747182 31.48525417151727 19.911482438456517 22-23 21.325009139340334 26.92964305108448 31.424778792471315 20.320569017103875 24-25 21.204208922309558 26.82809387023042 31.76591123120679 20.201785976253237 26-27 21.079954900329966 27.28601022905947 31.40295230106514 20.231082569545418 28-29 20.7207665524189 27.39639320949459 31.715289994900854 20.167550243185655 30-31 21.207883612719122 27.160818080395433 31.496355503184276 20.13494280370117 32-33 20.920644159875827 27.2968189518915 31.22971028895388 20.5528265992788 34-35 20.997070709938765 27.335742071041196 31.32956169491846 20.337625524101576 36-37 21.334271906111173 27.22633998024265 31.05074799912452 20.388640114521653 38-39 21.055682670534537 27.3433784400902 31.21038173140855 20.390557157966718 40-41 21.205646573345827 27.335150534276003 31.164286322723527 20.294916569654635 42-43 21.113100231555705 27.65806901235634 30.747687105175036 20.481143650912912 44-45 21.279233746935837 27.594007022724476 30.58114937959624 20.545609850743443 46-47 21.394110186736327 28.075991179003758 29.94725858201539 20.58264005224453 48-49 21.587897631013565 28.253570515914706 29.37370453448423 20.784827318587503 50-51 21.662667878133963 28.4867543087538 29.074505238649596 20.77607257446265 52-53 21.657724591987034 28.700333035592806 28.89128132930299 20.750661043117166 54-55 21.444982348542926 28.611686873562565 28.986839490047984 20.95649128784652 56-57 21.471009966211422 28.70136384716583 28.94081792971597 20.886808256906782 58-59 21.53300301920365 28.39139858220468 29.104082076909243 20.971516321682426 60-61 21.457996157377174 28.420147268993063 29.267109609396442 20.85474696423332 62-63 21.37151348230595 28.410564373396934 29.528213937552643 20.689708206744463 64-65 21.421320877935205 28.316155105672124 29.422447163936134 20.840076852456534 66-67 21.143771827706637 28.65782674124762 29.137799672525244 21.060601758520495 68-69 21.291656019004893 28.423814796937258 29.03226951361481 21.25225967044304 70-71 21.119992049745875 28.603287051496824 28.975955213568433 21.300765685188868 72-73 21.128391871811615 28.446411501367635 28.99571254152588 21.42948408529487 74-75 21.244333077789452 28.56968776323386 28.629787898577476 21.556191260399217 76-77 21.21309993658726 28.20541942322799 28.975718598862354 21.605762041322393 78-79 21.250485060147458 28.294623167419104 28.77577917222711 21.679112600206327 80-81 21.14270706152929 28.623399301513388 28.53147448820239 21.702419148754934 82-83 20.847012669579403 28.48697372266055 28.661241072199683 22.00477253556036 84-85 21.10726479851027 28.443166196196646 28.769340340300076 21.68022866499301 86-87 21.029486924671343 28.402874395449423 28.528635111729468 22.039003568149766 88-89 20.817125225967047 28.316628335084282 28.435290610182 22.43095582876667 90-91 20.784137873099674 28.349718345021135 28.657580665962286 22.208563115916906 92-93 20.700274478254695 28.190099853296108 28.54254412947802 22.56708153897118 94-95 20.495447533055074 28.122012739335776 28.494562594054347 22.887977133554806 96-97 20.807542330370918 28.161172473191552 28.24043839972742 22.79084679671011 98-99 20.5865754899467 28.431993090760667 28.143910937065584 22.83752048222705 100-101 20.099984026220664 28.981641986191555 27.818514201873075 23.099859785714706 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 168.0 1 115.0 2 82.5 3 107.0 4 94.0 5 80.5 6 152.5 7 339.5 8 563.0 9 734.5 10 765.0 11 756.5 12 844.5 13 909.0 14 965.5 15 1042.5 16 1062.5 17 1071.0 18 1090.0 19 1139.0 20 1217.0 21 1309.0 22 1497.0 23 1743.5 24 2039.0 25 2460.0 26 2938.0 27 3657.0 28 4376.0 29 5239.0 30 6237.5 31 7044.5 32 7860.0 33 8705.5 34 9912.0 35 10847.0 36 11873.5 37 13051.5 38 13757.5 39 14269.0 40 14453.5 41 14778.0 42 15063.0 43 15261.5 44 15836.5 45 16098.5 46 15929.0 47 15873.5 48 16589.5 49 16419.0 50 15153.0 51 14163.0 52 13038.0 53 12232.0 54 11491.0 55 10214.5 56 8699.0 57 7644.0 58 6892.5 59 6008.0 60 5068.0 61 4005.5 62 2874.5 63 1986.0 64 1398.5 65 1003.5 66 721.5 67 546.5 68 371.5 69 223.5 70 153.0 71 105.0 72 72.5 73 70.5 74 53.5 75 32.5 76 30.0 77 17.5 78 7.0 79 6.5 80 3.5 81 2.5 82 4.5 83 2.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04921585886406012 2 0.0 3 0.0 4 0.0 5 0.009937817655242908 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004850601474582848 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010647661773474546 24-25 4.7322941215442423E-4 26-27 0.002011225001656303 28-29 0.002011225001656303 30-31 0.0010647661773474546 32-33 0.0 34-35 0.0 36-37 1.1830735303860606E-4 38-39 2.3661470607721211E-4 40-41 0.0 42-43 0.012185657362976424 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.1830735303860606E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.5492205911581817E-4 84-85 2.3661470607721211E-4 86-87 0.0 88-89 0.0 90-91 0.008636436771818242 92-93 0.001892917648617697 94-95 0.0 96-97 0.0 98-99 0.0013013808834246665 100-101 0.014315189717671331 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 422628.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.45044526099069 #Duplication Level Percentage of deduplicated Percentage of total 1 84.73231633276077 58.846970973053494 2 9.283180845504441 12.894420863171671 3 2.5793713753264944 5.37415471529637 4 1.1136693633810097 3.0937933264134068 5 0.6223306625586696 2.1610570807133476 6 0.3756349049229694 1.5652806841482076 7 0.23800602133325416 1.1570736909473947 8 0.16653749464468834 0.9252882524578766 9 0.13438446228657247 0.8399754667765142 >10 0.7082646208533986 8.535728762774891 >50 0.03429919737538484 1.613392624214792 >100 0.010632751160692108 1.1588889859564178 >500 3.429919729255519E-4 0.15698002913572032 >1k 0.0010289759187766556 1.6769945449400168 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3007 0.7115004211741769 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2476 0.5858580122471772 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1557 0.3684090973622193 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 659 0.15592909130488278 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.3661470607721211E-4 0.0 0.0 0.0 0.0 6 2.3661470607721211E-4 0.0 0.0 0.0 0.0 7 2.3661470607721211E-4 0.0 0.0 0.0 0.0 8 2.3661470607721211E-4 0.0 0.0 0.0 0.0 9 2.3661470607721211E-4 0.0 0.0 0.0 0.0 10-11 2.3661470607721211E-4 0.0 0.0 0.0 0.0 12-13 2.3661470607721211E-4 0.0 0.0 0.0 0.0 14-15 2.3661470607721211E-4 0.0 0.0 0.0 0.0 16-17 2.3661470607721211E-4 0.0 0.0 0.0 0.0 18-19 3.5492205911581817E-4 0.0 0.0 0.0 0.0 20-21 4.7322941215442423E-4 0.0 0.0 0.0 0.0 22-23 4.7322941215442423E-4 0.0 0.0 0.0 0.0 24-25 4.7322941215442423E-4 0.0 0.0 5.915367651930303E-4 0.0 26-27 4.7322941215442423E-4 0.0 0.0 0.0011830735303860606 0.0 28-29 4.7322941215442423E-4 0.0 0.0 0.0028393764729265454 0.0 30-31 4.7322941215442423E-4 0.0 0.0 0.00662521177016194 0.0 32-33 4.7322941215442423E-4 0.0 0.0 0.012303964716015029 0.0 34-35 4.7322941215442423E-4 0.0 0.0 0.017864410308829515 0.0 36-37 4.7322941215442423E-4 0.0 0.0 0.02697407649280218 0.0 38-39 4.7322941215442423E-4 0.0 0.0 0.03892311914970139 0.0 40-41 4.7322941215442423E-4 0.0 0.0 0.06968303093973896 0.0 42-43 4.7322941215442423E-4 0.0 0.0 0.09665710743254115 0.0 44-45 7.098441182316363E-4 0.0 0.0 0.11795243097949024 0.0 46-47 7.098441182316363E-4 0.0 0.0 0.1760413413214458 0.0 48-49 7.098441182316363E-4 0.0 0.0 0.21129693252695042 0.0 50-51 7.098441182316363E-4 0.0 0.0 0.26205078698051243 0.0 52-53 7.098441182316363E-4 0.0 0.0 0.32132277085285404 0.0 54-55 7.098441182316363E-4 0.0 0.0 0.4018900782721448 0.0 56-57 7.098441182316363E-4 0.0 0.0 0.6211136034526819 0.0 58-59 7.098441182316363E-4 0.0 0.0 0.7939606462420852 0.0 60-61 7.098441182316363E-4 0.0 0.0 0.9379406948900688 2.3661470607721211E-4 62-63 7.098441182316363E-4 0.0 0.0 1.1352773597584638 2.3661470607721211E-4 64-65 7.098441182316363E-4 0.0 0.0 1.3323774099207815 2.3661470607721211E-4 66-67 7.098441182316363E-4 0.0 0.0 1.6223487322184047 2.3661470607721211E-4 68-69 7.098441182316363E-4 0.0 0.0 1.9539642427856174 2.3661470607721211E-4 70-71 7.098441182316363E-4 0.0 0.0 2.1724779238479233 2.3661470607721211E-4 72-73 7.098441182316363E-4 0.0 0.0 2.437959624066555 2.3661470607721211E-4 74-75 7.098441182316363E-4 0.0 0.0 2.900777989153582 2.3661470607721211E-4 76-77 7.098441182316363E-4 0.0 0.0 3.448777648428405 2.3661470607721211E-4 78-79 7.098441182316363E-4 0.0 0.0 3.953121894431983 2.3661470607721211E-4 80-81 7.098441182316363E-4 0.0 0.0 4.330995580037291 2.3661470607721211E-4 82-83 7.098441182316363E-4 0.0 0.0 4.930577245236946 2.3661470607721211E-4 84-85 7.098441182316363E-4 0.0 0.0 5.67709664291055 2.3661470607721211E-4 86-87 7.098441182316363E-4 0.0 0.0 6.3122888213748265 2.3661470607721211E-4 88-89 7.098441182316363E-4 0.0 0.0 7.108970536736799 2.3661470607721211E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1500 0.0 43.08008 1 GTATCAA 2570 0.0 40.859028 1 CATATAG 135 3.074092E-10 35.18365 3 GGACCGA 55 0.0025783388 34.54395 6 ATCAACG 3235 0.0 31.861053 3 TATCAAC 3285 0.0 31.665287 2 TCAACGC 3260 0.0 31.61672 4 CAACGCA 3305 0.0 31.186235 5 AACGCAG 3390 0.0 30.40428 6 TAAGGTG 140 5.7936995E-7 27.141672 5 TACCTGG 285 0.0 26.665504 2 ACGCAGA 3855 0.0 26.49355 7 CGCAGAG 3865 0.0 26.425001 8 GTCTAAG 170 1.11595E-7 25.154892 1 GCAGAGT 4080 0.0 24.916077 9 GAGTACT 2310 0.0 24.368704 12-13 GCACCCG 120 1.7074577E-4 23.751774 9 GTAAGGT 140 1.7766284E-5 23.748964 4 AGTACTT 2385 0.0 23.104452 12-13 GTACTTT 2480 0.0 22.695581 14-15 >>END_MODULE