FastQCFastQC Report
Thu 26 May 2016
SRR937297_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937297_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485409
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27530.5671505884728136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21040.43344890597413727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13620.2805881225935242No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7110.1464744164199675No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.11001032119305576No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA352.776286E-454.3254052
GTATCAA27450.038.983041
GGTATCA15700.033.6247371
ATCAACG33400.032.02193
TATCAAC33800.031.7835772
TCAACGC34300.031.320264
CAACGCA34800.030.8638765
AACGCAG35400.030.4624186
CGCAGAG39700.027.1629628
TCGGTTC350.00838638927.14586834-35
ACGCAGA39750.027.1287967
GTACATG26650.026.7688391
TACATGG25350.026.251922
ACATGGG25400.025.4516663
GCAGAGT41500.025.2979879
AGTACTT22300.023.53474212-13
GTACTAA1050.0021785422.6472871
GAGTACT21700.021.88730612-13
AGAGTAC37650.021.63471610-11
CCCTATA1553.8784878E-521.4672972
Produced by FastQC (version 0.11.5)