##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937297_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 485409 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94901001011518 33.0 31.0 34.0 30.0 34.0 2 32.05300684577336 33.0 31.0 34.0 30.0 34.0 3 32.09384251219075 34.0 31.0 34.0 30.0 34.0 4 35.701060342927306 37.0 35.0 37.0 35.0 37.0 5 35.692821929548074 37.0 35.0 37.0 35.0 37.0 6 35.74899311714451 37.0 35.0 37.0 35.0 37.0 7 35.71113844201488 37.0 35.0 37.0 35.0 37.0 8 35.69770647021378 37.0 35.0 37.0 35.0 37.0 9 37.36048569350795 39.0 37.0 39.0 35.0 39.0 10-11 37.27427179965761 39.0 37.0 39.0 34.5 39.0 12-13 37.177222713217105 39.0 37.0 39.0 34.0 39.0 14-15 38.39668506352375 40.0 38.0 41.0 34.0 41.0 16-17 38.354473238032256 40.0 38.0 41.0 34.0 41.0 18-19 38.32415962621212 40.0 38.0 41.0 34.0 41.0 20-21 38.26650103314937 40.0 38.0 41.0 34.0 41.0 22-23 38.14532384030787 40.0 38.0 41.0 34.0 41.0 24-25 38.01421069654663 40.0 38.0 41.0 33.0 41.0 26-27 37.848816152976156 40.0 38.0 41.0 33.0 41.0 28-29 37.67737516197681 40.0 37.0 41.0 33.0 41.0 30-31 37.63966675525176 40.0 37.0 41.0 33.0 41.0 32-33 37.47837905766065 40.0 37.0 41.0 33.0 41.0 34-35 37.28036562980909 40.0 36.5 41.0 32.0 41.0 36-37 37.33140918277164 40.0 37.0 41.0 33.0 41.0 38-39 37.31792982824793 40.0 37.0 41.0 32.5 41.0 40-41 37.15613122129997 40.0 37.0 41.0 31.5 41.0 42-43 36.930210399889575 40.0 37.0 41.0 31.0 41.0 44-45 36.685821647311855 40.0 36.0 41.0 31.0 41.0 46-47 36.3127496605955 40.0 36.0 41.0 30.0 41.0 48-49 35.990160874643855 39.0 36.0 41.0 29.0 41.0 50-51 35.08483361453949 38.5 35.0 40.0 27.0 40.5 52-53 35.19998187095831 38.5 35.0 40.0 27.5 40.5 54-55 35.55586526001784 39.0 35.0 40.5 28.0 41.0 56-57 35.36932772157088 39.0 35.0 41.0 26.5 41.0 58-59 35.1492401253376 39.0 35.0 40.5 26.0 41.0 60-61 34.88268140887375 38.5 35.0 40.0 26.0 41.0 62-63 34.58384681783815 38.0 34.0 40.0 25.5 41.0 64-65 34.24408900535425 38.0 34.0 40.0 24.5 41.0 66-67 33.86432781427621 37.0 34.0 40.0 22.5 41.0 68-69 33.4030065367556 36.5 33.5 39.5 22.0 41.0 70-71 32.976350871121056 36.0 33.0 39.0 20.5 40.5 72-73 32.57607399121153 36.0 32.5 39.0 19.0 40.0 74-75 32.12443836022818 35.0 32.0 38.0 18.0 40.0 76-77 31.66407091751492 35.0 32.0 37.0 14.5 39.0 78-79 31.199008465026402 35.0 31.0 37.0 9.0 39.0 80-81 30.7469175478823 35.0 31.0 36.0 7.0 38.5 82-83 30.328264412073118 34.5 31.0 36.0 2.0 37.0 84-85 29.869978718977194 34.0 30.0 35.0 2.0 37.0 86-87 29.451612969681236 34.0 30.0 35.0 2.0 36.5 88-89 29.08481301335575 34.0 29.0 35.0 2.0 36.0 90-91 28.673765834584856 34.0 29.0 35.0 2.0 36.0 92-93 28.270119631074003 34.0 29.0 35.0 2.0 35.0 94-95 27.792164957798477 34.0 28.0 35.0 2.0 35.0 96-97 27.233598882591792 33.5 26.5 35.0 2.0 35.0 98-99 26.587107985224833 33.0 25.5 35.0 2.0 35.0 100-101 24.846403754359727 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.018376134001595545 1101 2 0.02356929674055408 1101 3 0.12873558669647167 1101 4 0.22283148720335078 1101 5 0.19300419618593878 1101 6 0.06828862299170169 1101 7 0.10750514995149274 1101 8 0.0761163971253822 1101 9 0.15497684716994087 1101 10-11 -0.10980635668924066 1101 12-13 -0.022926565307798796 1101 14-15 -0.005572567995962174 1101 16-17 -0.1781546596918986 1101 18-19 -0.02591621633439445 1101 20-21 -0.13347099624125036 1101 22-23 -0.13686848260673656 1101 24-25 -0.14464181135603837 1101 26-27 0.013474006902221447 1101 28-29 -0.0460128554613064 1101 30-31 -0.13443506826119034 1101 32-33 -0.29390224699921674 1101 34-35 -0.3519564937580739 1101 36-37 -0.11966721044430528 1101 38-39 0.07414718195196457 1101 40-41 0.15762778774870156 1101 42-43 0.04298705592982088 1101 44-45 0.25116898208213456 1101 46-47 0.13606684171352867 1101 48-49 0.2963286410323249 1101 50-51 0.11556529192436216 1101 52-53 0.31328325105791777 1101 54-55 0.3666862010839793 1101 56-57 0.3239402830593505 1101 58-59 0.1122879325420385 1101 60-61 0.21785247227413151 1101 62-63 0.2327644117288159 1101 64-65 -0.017107250680354014 1101 66-67 0.18899420496617125 1101 68-69 0.14579652956155087 1101 70-71 0.007817094332288832 1101 72-73 0.057583578543479064 1101 74-75 -0.08071666282410561 1101 76-77 -0.22182018401230863 1101 78-79 0.04949840667388017 1101 80-81 0.2882119918327817 1101 82-83 0.376587187259787 1101 84-85 0.0610108906358775 1101 86-87 0.09343777262179387 1101 88-89 0.30401435436587576 1101 90-91 0.2882134238538079 1101 92-93 -0.030878858890009298 1101 94-95 -0.18891719360886228 1101 96-97 -0.2754289264907861 1101 98-99 -0.06922728570021519 1101 100-101 0.3152503124648405 1102 1 -0.3014595424155999 1102 2 -0.44088290077489845 1102 3 -0.23230493664845397 1102 4 -0.2958177936017279 1102 5 -0.10180443339488221 1102 6 -0.14377499824284 1102 7 -0.11192164228628343 1102 8 0.067033425927292 1102 9 -0.00816913619311066 1102 10-11 -0.11279547304554427 1102 12-13 0.049742890611106816 1102 14-15 0.07696015053933536 1102 16-17 0.05363801403508717 1102 18-19 0.03435513553471026 1102 20-21 -0.16863655070306294 1102 22-23 0.07719293738464472 1102 24-25 0.09742354105136997 1102 26-27 -0.006882437799440311 1102 28-29 0.02703000307153758 1102 30-31 -0.0691369969393989 1102 32-33 -0.2635580721431694 1102 34-35 -0.2303946725642234 1102 36-37 0.011247366915746682 1102 38-39 0.23117909239599044 1102 40-41 0.13865944041224765 1102 42-43 -0.05146358732094569 1102 44-45 -0.11664838802619215 1102 46-47 -0.4703522714457833 1102 48-49 -0.3240744316099651 1102 50-51 -0.20871371248697557 1102 52-53 -0.03987601305461652 1102 54-55 0.11409354858596998 1102 56-57 0.05422228732789591 1102 58-59 -0.0036210406197199063 1102 60-61 -0.10480276780921827 1102 62-63 -0.14147219790181964 1102 64-65 -0.18097034397035117 1102 66-67 -0.14998713248490958 1102 68-69 -0.11882208800214755 1102 70-71 -0.14686004192871138 1102 72-73 0.054504112654534254 1102 74-75 0.17210849756211388 1102 76-77 0.19145247824091882 1102 78-79 0.12188291406674878 1102 80-81 0.33740889189608936 1102 82-83 0.30416034826770755 1102 84-85 0.2657435634277796 1102 86-87 0.024001313615841013 1102 88-89 0.34723807623589664 1102 90-91 0.2539715827956961 1102 92-93 0.03721120253503685 1102 94-95 -0.22594091573160924 1102 96-97 0.11999054174851054 1102 98-99 0.40894224540466695 1102 100-101 0.7003124388252964 1103 1 0.1167588923759304 1103 2 -0.06712164288422073 1103 3 0.046504502662621405 1103 4 0.09764789076542257 1103 5 0.01429561520699707 1103 6 -0.01815696338346129 1103 7 -0.005519164588271508 1103 8 -0.021856698199904656 1103 9 0.2391944614289585 1103 10-11 0.1285135564989801 1103 12-13 0.00892230279920625 1103 14-15 -0.005447823018961628 1103 16-17 -0.02187804240009683 1103 18-19 -0.13899820865922408 1103 20-21 -0.004369334450373685 1103 22-23 -0.17378159437689789 1103 24-25 -0.21851431125879373 1103 26-27 -0.13048370857459446 1103 28-29 -0.3072368853679066 1103 30-31 -0.2423656295733494 1103 32-33 -0.04401726723338584 1103 34-35 -0.2657550319615538 1103 36-37 -0.0946149315681879 1103 38-39 -0.009153623793274335 1103 40-41 0.19870912549773578 1103 42-43 0.35322043634432276 1103 44-45 0.2974688180357816 1103 46-47 0.43363122957114797 1103 48-49 0.3355884338483506 1103 50-51 0.5673285554675758 1103 52-53 0.330805225623358 1103 54-55 0.4253237176663376 1103 56-57 0.4482915756453494 1103 58-59 0.5113571740523355 1103 60-61 0.43101011639146947 1103 62-63 0.5289130111646472 1103 64-65 0.41193834293807186 1103 66-67 0.726754375370497 1103 68-69 0.6970231214348814 1103 70-71 0.5411508300687728 1103 72-73 0.791163182605608 1103 74-75 0.7717858153086361 1103 76-77 0.8179285286329403 1103 78-79 0.7175466574944487 1103 80-81 0.6883878420100409 1103 82-83 0.46919222454243936 1103 84-85 0.5239093200563794 1103 86-87 0.6056909485886699 1103 88-89 0.5759248706357063 1103 90-91 0.7369794545893633 1103 92-93 0.8500882768445983 1103 94-95 0.900624214346113 1103 96-97 0.9628219074897437 1103 98-99 0.8759385781721676 1103 100-101 0.958708377361738 1104 1 0.01699629703243133 1104 2 0.011744780587434889 1104 3 0.11259880126609545 1104 4 -0.005952132396004117 1104 5 0.20202075922020413 1104 6 0.029709991748283926 1104 7 0.21098405607874327 1104 8 0.185453891311127 1104 9 0.2032223042751511 1104 10-11 0.20785636847204358 1104 12-13 0.4433429950803074 1104 14-15 0.4450735723519088 1104 16-17 0.23860469794799144 1104 18-19 0.3008408544960304 1104 20-21 0.19335996809230238 1104 22-23 0.3552000221154472 1104 24-25 0.19502951034204585 1104 26-27 0.40538492400616377 1104 28-29 0.10683157771329377 1104 30-31 0.005235320045279934 1104 32-33 -0.046044769676740316 1104 34-35 0.2644934445602942 1104 36-37 0.1712273987524 1104 38-39 0.1890889149801822 1104 40-41 -0.010672832801155607 1104 42-43 0.06432283267498207 1104 44-45 -0.004473759164596913 1104 46-47 -0.10338933321650501 1104 48-49 0.11925102796359255 1104 50-51 0.07788893924544738 1104 52-53 0.09201965659791256 1104 54-55 0.14564506495969454 1104 56-57 0.3224188547212705 1104 58-59 0.3489244869579977 1104 60-61 0.3700551987110501 1104 62-63 0.37846373206414796 1104 64-65 0.27011757380312673 1104 66-67 0.5803443092726823 1104 68-69 0.4982024242407519 1104 70-71 0.40589773003420504 1104 72-73 0.3377115154213115 1104 74-75 0.3718545977368315 1104 76-77 0.6314140957282675 1104 78-79 0.296818405989395 1104 80-81 0.35144694050402236 1104 82-83 0.10552092734444685 1104 84-85 0.5085675047266669 1104 86-87 0.6730459659334223 1104 88-89 0.5232843798941396 1104 90-91 0.40204675433511383 1104 92-93 0.5112707731203479 1104 94-95 0.22576770115901112 1104 96-97 0.20458249200001077 1104 98-99 0.11285719557517382 1104 100-101 0.282774506753416 1105 1 0.28868069912135397 1105 2 0.23589471331872858 1105 3 0.25403476177265816 1105 4 0.27610195101718915 1105 5 0.29102146780468985 1105 6 0.13332564553453352 1105 7 0.2676458427729642 1105 8 0.384903255614077 1105 9 0.17954559360548927 1105 10-11 0.3649639078733955 1105 12-13 0.18812459810443727 1105 14-15 0.33415668899989726 1105 16-17 0.3228201201176333 1105 18-19 0.33811333488509376 1105 20-21 0.38431724388149036 1105 22-23 0.23784188728460975 1105 24-25 0.13711131283115208 1105 26-27 0.2865066101250804 1105 28-29 0.17481354782788827 1105 30-31 0.5186984252685178 1105 32-33 0.2258331665007134 1105 34-35 0.36664354585354175 1105 36-37 0.3955880339187914 1105 38-39 0.31369365281953066 1105 40-41 0.3219014515129075 1105 42-43 0.23493873930414821 1105 44-45 0.6967423840755984 1105 46-47 0.6865871617562931 1105 48-49 0.8972034373069206 1105 50-51 0.7102131900031949 1105 52-53 0.6572671773325922 1105 54-55 0.7639942816148633 1105 56-57 0.9441830115837817 1105 58-59 0.9662248737140189 1105 60-61 1.0110416606894077 1105 62-63 0.8254603639467035 1105 64-65 0.9680061793275954 1105 66-67 0.9159246149941183 1105 68-69 1.1062350253701112 1105 70-71 1.0758643610115186 1105 72-73 1.424611442689958 1105 74-75 1.1469570615506406 1105 76-77 1.300466122061394 1105 78-79 1.1806355225187595 1105 80-81 1.0806662177478437 1105 82-83 1.2384882107189341 1105 84-85 1.3415351002529086 1105 86-87 1.3230801465506694 1105 88-89 1.0057711056407257 1105 90-91 1.2476377668617609 1105 92-93 1.5067008714169354 1105 94-95 1.5233091939311372 1105 96-97 1.3373687273561892 1105 98-99 1.3149910649771215 1105 100-101 1.3904574979163975 1106 1 -0.21242575540275155 1106 2 0.12322797247634298 1106 3 -0.11071817125903038 1106 4 -0.088824058699835 1106 5 -0.3871053479551776 1106 6 -0.12203045974554527 1106 7 -0.1396530482999907 1106 8 -0.19586174333510087 1106 9 -0.16363456686132594 1106 10-11 -0.427384248241772 1106 12-13 -0.3147064419628194 1106 14-15 -0.37980936324454007 1106 16-17 -0.292934185164917 1106 18-19 -0.35731496809800944 1106 20-21 -0.4258361762374321 1106 22-23 -0.09523801233090978 1106 24-25 -0.17892980524423763 1106 26-27 -0.17045910832859335 1106 28-29 -0.30468304627019904 1106 30-31 -0.12526428906841858 1106 32-33 0.008346858102790122 1106 34-35 0.008350081970938561 1106 36-37 -0.06545425860553422 1106 38-39 -0.24383580112353087 1106 40-41 0.33490692815373535 1106 42-43 -0.09083485166618743 1106 44-45 0.374099626716756 1106 46-47 0.40098899636915775 1106 48-49 0.17331020089653038 1106 50-51 0.009731665939355594 1106 52-53 0.2119995884144501 1106 54-55 0.4119655756103384 1106 56-57 0.13349181747827288 1106 58-59 0.02526256734356025 1106 60-61 0.33497013056787495 1106 62-63 0.6933750371408394 1106 64-65 0.4293513560512494 1106 66-67 0.5466253283173117 1106 68-69 0.47146700994156276 1106 70-71 0.5359701850419825 1106 72-73 0.6869926070015424 1106 74-75 0.6775700002691316 1106 76-77 0.5476072660982823 1106 78-79 0.6514751916950452 1106 80-81 1.1207736489189557 1106 82-83 0.5877476897581104 1106 84-85 0.43079321940384574 1106 86-87 0.520304120144111 1106 88-89 0.634892494210245 1106 90-91 0.46241048008605645 1106 92-93 0.4435587878208942 1106 94-95 0.5157710861538121 1106 96-97 0.7840271110672035 1106 98-99 1.0798756599158637 1106 100-101 0.9953481505391295 1107 1 -0.007941292125913435 1107 2 0.02988404592267102 1107 3 -0.11449641984660275 1107 4 -0.05169482141169368 1107 5 -0.20396622366138928 1107 6 -0.09007424505627881 1107 7 -0.22265092965592004 1107 8 -0.105113155764478 1107 9 -0.16467623352799166 1107 10-11 -0.11741779343952174 1107 12-13 -0.18463935156734124 1107 14-15 -0.06652369940272962 1107 16-17 0.11027909167124506 1107 18-19 -0.10910698787201767 1107 20-21 0.01162851302810708 1107 22-23 -0.1991486761727188 1107 24-25 -0.15730198038548338 1107 26-27 0.24256172500474094 1107 28-29 -0.18435289090296436 1107 30-31 -0.011501930311347053 1107 32-33 -0.19672905997631318 1107 34-35 -0.19949772876352512 1107 36-37 -0.5814941597354704 1107 38-39 -0.34882344236645935 1107 40-41 -0.21087343907790768 1107 42-43 0.20024918788183044 1107 44-45 0.012305841406018203 1107 46-47 0.2061885020188683 1107 48-49 0.3266823760377733 1107 50-51 0.5081515105721266 1107 52-53 0.4065970460415613 1107 54-55 0.5238034993391523 1107 56-57 0.2941556592861829 1107 58-59 -0.055916811187515236 1107 60-61 0.42256723508765504 1107 62-63 0.517439302677559 1107 64-65 0.7730219068987125 1107 66-67 0.6170084497862405 1107 68-69 0.45928480655173587 1107 70-71 0.6057706793922719 1107 72-73 0.40830263525013066 1107 74-75 0.25416781100359387 1107 76-77 0.07370189886664136 1107 78-79 0.1713904459323352 1107 80-81 0.25330366304325125 1107 82-83 0.3429295400405934 1107 84-85 0.22640586064678203 1107 86-87 0.5301028489576716 1107 88-89 0.31410329929499525 1107 90-91 0.10338858743068968 1107 92-93 -0.028607525738426176 1107 94-95 0.30954756920466053 1107 96-97 0.26305253479601376 1107 98-99 0.5227693746051294 1107 100-101 0.9891069782227468 1108 1 -0.16246715419487856 1108 2 -0.4077237126980222 1108 3 -0.24617745432935934 1108 4 -0.06365602830825168 1108 5 -0.19545329262690814 1108 6 -0.2161518312631756 1108 7 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2316 5 -2.1499833805241337 2316 6 -1.0208340489778465 2316 7 -0.8683004394598441 2316 8 -1.197024920470362 2316 9 -0.46265417470446124 2316 10-11 -0.8961861757924581 2316 12-13 -0.7741614103908674 2316 14-15 -1.2303411013635142 2316 16-17 -0.8525885553875838 2316 18-19 -1.0953508604210356 2316 20-21 -0.9696214869718958 2316 22-23 -0.5633337251923294 2316 24-25 -1.3236622745031283 2316 26-27 -0.9106551867599677 2316 28-29 -0.5082805869813996 2316 30-31 -0.41410609697802414 2316 32-33 -1.1399582266429746 2316 34-35 -1.0967097385674478 2316 36-37 -0.8336081303237037 2316 38-39 -1.064540354131175 2316 40-41 -1.1827484390779048 2316 42-43 -1.7079003219220894 2316 44-45 -2.063919648790062 2316 46-47 -1.692065174451713 2316 48-49 -1.3559769376877142 2316 50-51 -1.0538276560945476 2316 52-53 -1.3940156990564745 2316 54-55 -1.4175874320333932 2316 56-57 -1.1752070858118557 2316 58-59 -1.3185393602071187 2316 60-61 -1.4134928139319598 2316 62-63 -1.4497788345773444 2316 64-65 -1.3272538283954063 2316 66-67 -1.1648236580568962 2316 68-69 -1.3599553895266965 2316 70-71 -1.3513678009998884 2316 72-73 -2.148376286318495 2316 74-75 -1.960017238016011 2316 76-77 -1.9749500619176708 2316 78-79 -1.643805632499042 2316 80-81 -1.4451951114665569 2316 82-83 -1.2747481560378375 2316 84-85 -1.380855168764981 2316 86-87 -1.4959388177089927 2316 88-89 -1.3483660634501042 2316 90-91 -2.146305040020291 2316 92-93 -1.536542574758034 2316 94-95 -1.8104585582463208 2316 96-97 -1.44013373971379 2316 98-99 -1.7135357724536924 2316 100-101 -1.8160094433458802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1836.0 3 334.0 4 251.0 5 299.0 6 373.0 7 423.0 8 470.0 9 600.0 10 775.0 11 993.0 12 1388.0 13 1855.0 14 2152.0 15 2885.0 16 3964.0 17 5486.0 18 4726.0 19 2844.0 20 2684.0 21 2698.0 22 2887.0 23 3102.0 24 3535.0 25 3882.0 26 4483.0 27 5168.0 28 6128.0 29 7596.0 30 9224.0 31 11330.0 32 15281.0 33 20475.0 34 28571.0 35 42972.0 36 66526.0 37 103174.0 38 98564.0 39 15472.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19546309113071 19.578745119104187 15.254219779765718 21.97157200999938 2 14.46121054653454 20.262382842673563 41.93891502711812 23.33749158367377 3 18.444973655348758 25.59035358219097 30.82726955401015 25.137403208450117 4 11.876554225427748 16.916995067786946 38.88723655620823 32.319214150577075 5 12.7890262942678 37.876797766267664 35.059436775627205 14.274739163837328 6 29.944902387012736 37.14516332138073 18.5715494836018 14.33838480800474 7 27.125960762292493 33.56077137625134 21.67281540770592 17.64045245375025 8 25.629295341986126 34.97856070818822 20.689071431963022 18.703072517862633 9 26.088760621299578 16.473645964938356 21.247966573356123 36.18962684040594 10-11 23.71129550752256 27.33434776594273 28.194172311770792 20.76018441476392 12-13 24.92969756532655 24.86486653893165 28.89770737669717 21.30772851904463 14-15 22.02050103785085 25.77998800613937 27.707985619753334 24.49152533625645 16-17 20.773227109399432 29.324886892774455 28.112732523991742 21.789153473834375 18-19 20.613871666596136 27.43276424937582 31.339905270795683 20.613458813232363 20-21 21.58781374215677 26.43892781904753 31.40740006750417 20.56585837129153 22-23 21.199212649628052 26.56677872750225 31.2359687413232 20.9980398815465 24-25 20.857476080876054 26.5616323834491 31.516374407827513 21.064517127847328 26-27 20.790213067272774 26.857913175241173 31.223614319541042 21.128259437945008 28-29 20.537250518393286 26.971416893479855 31.47310392946755 21.01822865865931 30-31 20.78758151719525 26.824240772738627 31.36886956629446 21.019308143771664 32-33 20.537181551674305 26.872909698996654 31.237458193979933 21.352450555349105 34-35 20.76525067115029 26.985350809560526 31.177808122750577 21.07159039653861 36-37 20.78595264218852 26.803476745830313 31.158595305505465 21.2519753064757 38-39 20.690744217393593 27.05045015104805 31.184738935098366 21.074066696459994 40-41 20.937757383221555 27.005447493306743 30.9904364393747 21.066358684096993 42-43 20.743761307774793 27.090874000039218 30.862110426839152 21.303254265346837 44-45 20.752113144174803 27.296795989335447 30.575227313310975 21.37586355317877 46-47 20.963633586399645 27.38875196611471 30.14812429560457 21.49949015188107 48-49 20.865541525989677 27.640022418863225 29.891611195806888 21.60282485934021 50-51 21.01345681216924 27.72348970843402 29.727715541756165 21.535337937640573 52-53 21.161367439143095 27.892760962397382 29.473497325010918 21.472374273448604 54-55 20.97492291477631 27.728486590216296 29.633242596257176 21.663347898750224 56-57 20.989611392172524 27.89980040167357 29.39575580063285 21.71483240552105 58-59 21.140831553202688 27.53361609031712 29.66120759935193 21.664344757128262 60-61 21.055798376568088 27.43104542616378 29.869083065427503 21.64407313184063 62-63 20.931910220897542 27.651513587820293 29.927571530595813 21.489004660686348 64-65 20.833466637081834 27.578705113187784 30.027916385008744 21.559911864721638 66-67 20.7877554306428 27.711192811300773 29.737813953392354 21.76323780466407 68-69 20.7427161370422 27.53135588060151 29.823304852537312 21.90262312981897 70-71 20.645587453619367 27.614319552888112 29.74336479561558 21.99672819787694 72-73 20.624590341609938 27.607285339003607 29.653229011297537 22.114895308088915 74-75 20.715244091237487 27.621323149963878 29.602538961709158 22.060893797089484 76-77 20.873900141917172 27.286904915494777 29.605986324345245 22.23320861824281 78-79 20.74445247187532 27.415832387243267 29.51955826194654 22.320156878934874 80-81 20.625794653324746 27.607701325935835 29.51373408629316 22.252769934446263 82-83 20.483604095337675 27.36463017994259 29.615847912230375 22.535917812489355 84-85 20.668844202954638 27.338090888919847 29.668054717049287 22.32501019107623 86-87 20.642377469521538 27.343562138079573 29.650667775756144 22.36339261664274 88-89 20.644286352701005 26.998597403079934 29.472183891912312 22.88493235230675 90-91 20.43801420899022 26.946099132681915 29.885527222889603 22.730359435438263 92-93 20.456684818733926 27.00102802079162 29.891872029990136 22.650415130484326 94-95 20.242261853769705 26.86503541758483 29.80082962712193 23.09187310152353 96-97 20.253674042272127 26.985530878467635 29.616702443857328 23.144092635402906 98-99 20.196978750940463 26.98111435521608 29.704820850065587 23.11708604377787 100-101 20.18658410732714 26.963983488132094 29.84355005159959 23.005882352941175 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 682.0 1 413.0 2 140.5 3 129.0 4 112.5 5 112.0 6 180.5 7 384.0 8 627.5 9 787.0 10 782.0 11 726.5 12 779.0 13 844.0 14 887.5 15 938.0 16 987.0 17 986.0 18 1045.5 19 1134.5 20 1217.0 21 1391.5 22 1629.0 23 1914.5 24 2263.5 25 2745.5 26 3417.0 27 4276.5 28 5083.5 29 5963.0 30 7053.5 31 8037.0 32 8908.5 33 10015.5 34 11466.5 35 12488.5 36 13634.0 37 15184.5 38 15919.0 39 16194.0 40 16539.5 41 17003.5 42 17313.0 43 17702.0 44 18319.0 45 18733.5 46 18526.0 47 18312.5 48 19119.0 49 18960.0 50 17646.0 51 16403.5 52 15084.0 53 14164.5 54 13093.5 55 11541.5 56 10049.5 57 8807.0 58 7904.0 59 7011.5 60 5939.0 61 4635.5 62 3341.5 63 2347.0 64 1623.5 65 1171.5 66 838.5 67 643.0 68 475.5 69 299.0 70 195.0 71 136.5 72 103.0 73 76.5 74 53.0 75 43.0 76 31.0 77 18.0 78 14.5 79 15.0 80 8.5 81 4.5 82 3.5 83 2.5 84 2.0 85 1.5 86 2.0 87 1.5 88 2.0 89 3.0 90 2.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2840903238300073 2 0.25360057188886076 3 0.25751479680022415 4 0.2564847376130232 5 0.24659616941589466 6 0.2051877900904186 7 0.21178016888850434 8 0.20972005051410253 9 0.2070418966273802 10-11 0.21960861871123116 12-13 0.2210507015733124 14-15 0.2048787723342583 16-17 0.21085311562002354 18-19 0.2009645474228949 20-21 0.2048787723342583 22-23 0.20590883152145925 24-25 0.19859541129233285 26-27 0.20765993213970077 28-29 0.20178859477265562 30-31 0.20642386111505967 32-33 0.21198618072594452 34-35 0.20178859477265562 36-37 0.20405472498449762 38-39 0.1978743698612922 40-41 0.1990074349672132 42-43 0.1962262751617708 44-45 0.20611484335889943 46-47 0.1959172574056105 48-49 0.20508478417169848 50-51 0.20755692622098065 52-53 0.2089990090830619 54-55 0.21476734053138694 56-57 0.19282707984400782 58-59 0.18417458267152031 60-61 0.19396014494992883 62-63 0.19179702065680695 64-65 0.19086996738832612 66-67 0.20230362436625607 68-69 0.19169401473808687 70-71 0.19911044088593333 72-73 0.20838097357074137 74-75 0.19756535210513196 76-77 0.2001405000731342 78-79 0.19756535210513196 80-81 0.18973690228240517 82-83 0.20693889070866012 84-85 0.18880984901392434 86-87 0.1910759792257663 88-89 0.19581425148689044 90-91 0.1909729733070462 92-93 0.20250963620369625 94-95 0.20075853558545473 96-97 0.19138499698192657 98-99 0.19375413311248865 100-101 0.187264760233123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 485409.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.22849239619653 #Duplication Level Percentage of deduplicated Percentage of total 1 85.51815184327626 60.91329028305583 2 8.889737668720498 12.664052238812795 3 2.410468275293177 5.150820636539791 4 1.004234387053333 2.861204056089097 5 0.5516129297766439 1.9645278687119687 6 0.3566475679961071 1.524208115107962 7 0.25050319911086866 1.249007564916399 8 0.18391653028284508 1.0480077743029181 9 0.12705819979275135 0.8145147616031169 >10 0.6735330911321383 8.303681442690076 >50 0.025168295202806702 1.181533336795367 >100 0.007521558755719889 0.787139342400866 >500 5.785814427476593E-4 0.2565414872484421 >1k 8.67872164121489E-4 1.2814710917253507 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2753 0.5671505884728136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2104 0.43344890597413727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1362 0.2805881225935242 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 711 0.1464744164199675 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 534 0.11001032119305576 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.0601183744017933E-4 0.0 0.0 0.0 0.0 6 2.0601183744017933E-4 0.0 0.0 0.0 0.0 7 2.0601183744017933E-4 0.0 0.0 0.0 0.0 8 2.0601183744017933E-4 0.0 0.0 0.0 0.0 9 2.0601183744017933E-4 0.0 0.0 0.0 0.0 10-11 2.0601183744017933E-4 0.0 0.0 0.0 0.0 12-13 2.0601183744017933E-4 0.0 0.0 0.0 0.0 14-15 2.0601183744017933E-4 0.0 0.0 0.0 0.0 16-17 2.0601183744017933E-4 0.0 0.0 2.0601183744017933E-4 0.0 18-19 2.0601183744017933E-4 0.0 0.0 2.0601183744017933E-4 0.0 20-21 2.0601183744017933E-4 0.0 0.0 2.0601183744017933E-4 0.0 22-23 2.0601183744017933E-4 0.0 0.0 3.09017756160269E-4 0.0 24-25 2.0601183744017933E-4 0.0 0.0 4.1202367488035865E-4 0.0 26-27 2.0601183744017933E-4 0.0 0.0 0.0013390769433611655 0.0 28-29 2.0601183744017933E-4 0.0 0.0 0.004223242667523676 0.0 30-31 2.0601183744017933E-4 0.0 0.0 0.009785562278408516 0.0 32-33 2.0601183744017933E-4 0.0 0.0 0.01575990556417372 0.0 34-35 2.0601183744017933E-4 0.0 0.0 0.022146272524819273 0.0 36-37 2.0601183744017933E-4 0.0 0.0 0.03265287623426842 0.0 38-39 2.0601183744017933E-4 0.0 0.0 0.049133823229482766 0.0 40-41 2.0601183744017933E-4 0.0 0.0 0.07673940944646679 0.0 42-43 2.0601183744017933E-4 0.0 0.0 0.09898868789000616 0.0 44-45 2.0601183744017933E-4 0.0 0.0 0.12185600184586606 0.0 46-47 2.0601183744017933E-4 0.0 0.0 0.18417458267152031 0.0 48-49 2.0601183744017933E-4 0.0 0.0 0.2221837666792334 0.0 50-51 2.0601183744017933E-4 0.0 0.0 0.2673003590786327 0.0 52-53 2.0601183744017933E-4 0.0 0.0 0.3259107268303637 0.0 54-55 2.0601183744017933E-4 0.0 0.0 0.40368019546403133 0.0 56-57 4.1202367488035865E-4 0.0 0.0 0.6196836070200593 0.0 58-59 4.1202367488035865E-4 0.0 0.0 0.7852141184032435 0.0 60-61 4.1202367488035865E-4 0.0 0.0 0.9204608896827211 0.0 62-63 4.1202367488035865E-4 0.0 0.0 1.1206013897558553 0.0 64-65 4.1202367488035865E-4 0.0 0.0 1.305187996102256 0.0 66-67 4.1202367488035865E-4 0.0 0.0 1.5974157875111503 0.0 68-69 4.1202367488035865E-4 0.0 0.0 1.932288029270162 0.0 70-71 4.1202367488035865E-4 0.0 0.0 2.150557571038032 0.0 72-73 4.1202367488035865E-4 0.0 0.0 2.414046711124021 0.0 74-75 4.1202367488035865E-4 0.0 0.0 2.838740114006951 0.0 76-77 4.1202367488035865E-4 0.0 0.0 3.358095956193643 0.0 78-79 4.1202367488035865E-4 0.0 0.0 3.8481981174638293 0.0 80-81 4.1202367488035865E-4 0.0 0.0 4.208821838902863 0.0 82-83 4.1202367488035865E-4 0.0 0.0 4.799354770925138 0.0 84-85 4.1202367488035865E-4 0.0 0.0 5.525031468308169 0.0 86-87 4.1202367488035865E-4 0.0 0.0 6.133281418350299 0.0 88-89 4.1202367488035865E-4 0.0 0.0 6.947234188076447 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAATA 35 2.776286E-4 54.325405 2 GTATCAA 2745 0.0 38.98304 1 GGTATCA 1570 0.0 33.624737 1 ATCAACG 3340 0.0 32.0219 3 TATCAAC 3380 0.0 31.783577 2 TCAACGC 3430 0.0 31.32026 4 CAACGCA 3480 0.0 30.863876 5 AACGCAG 3540 0.0 30.462418 6 CGCAGAG 3970 0.0 27.162962 8 TCGGTTC 35 0.008386389 27.145868 34-35 ACGCAGA 3975 0.0 27.128796 7 GTACATG 2665 0.0 26.768839 1 TACATGG 2535 0.0 26.25192 2 ACATGGG 2540 0.0 25.451666 3 GCAGAGT 4150 0.0 25.297987 9 AGTACTT 2230 0.0 23.534742 12-13 GTACTAA 105 0.00217854 22.647287 1 GAGTACT 2170 0.0 21.887306 12-13 AGAGTAC 3765 0.0 21.634716 10-11 CCCTATA 155 3.8784878E-5 21.467297 2 >>END_MODULE